##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1948368
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95977710576236	33.0	31.0	34.0	30.0	34.0
2	32.22084175063438	33.0	31.0	34.0	30.0	34.0
3	32.14352062854656	33.0	31.0	34.0	30.0	34.0
4	35.81990876466869	37.0	35.0	37.0	35.0	37.0
5	35.843674295615614	37.0	35.0	37.0	35.0	37.0
6	35.58519540456423	37.0	35.0	37.0	33.0	37.0
7	35.92662833715191	37.0	35.0	37.0	35.0	37.0
8	36.01328753089766	37.0	35.0	37.0	35.0	37.0
9	37.802063573205885	39.0	38.0	39.0	35.0	39.0
10	37.227012042899496	39.0	37.0	39.0	34.0	39.0
11	37.10299645652156	39.0	37.0	39.0	33.0	39.0
12	37.09066972974305	39.0	37.0	39.0	33.0	39.0
13	37.09324624506253	39.0	37.0	39.0	33.0	39.0
14	38.31338894911023	40.0	38.0	41.0	33.0	41.0
15	38.288661587544034	40.0	38.0	41.0	33.0	41.0
16	38.27450461103857	40.0	38.0	41.0	33.0	41.0
17	38.19543535923398	40.0	37.0	41.0	33.0	41.0
18	37.95243044435138	39.0	37.0	41.0	33.0	41.0
19	37.73261878659473	39.0	37.0	41.0	33.0	41.0
20	37.45525177995122	39.0	35.0	41.0	33.0	41.0
21	37.274320354265726	39.0	35.0	40.0	33.0	41.0
22	37.35963637259491	39.0	35.0	40.0	33.0	41.0
23	37.28867544529575	39.0	35.0	40.0	33.0	41.0
24	37.20923408719503	39.0	35.0	41.0	33.0	41.0
25	37.13615189738284	39.0	35.0	40.0	33.0	41.0
26	37.06233678647976	39.0	35.0	40.0	33.0	41.0
27	36.98655952058338	39.0	35.0	40.0	32.0	41.0
28	36.84199186190699	39.0	35.0	40.0	32.0	41.0
29	36.719702848743154	38.0	35.0	40.0	31.0	41.0
30	36.57417951844826	38.0	35.0	40.0	31.0	41.0
31	36.31640224023388	38.0	35.0	40.0	30.0	41.0
32	35.82136588159937	38.0	35.0	40.0	28.0	41.0
33	35.27924396212625	38.0	35.0	40.0	24.0	41.0
34	34.74085183086563	38.0	34.0	40.0	21.0	41.0
35	34.42677666642031	38.0	34.0	40.0	18.0	41.0
36	34.120507008942866	38.0	34.0	40.0	15.0	41.0
37	34.02043043203337	38.0	33.0	40.0	15.0	41.0
38	33.905140609987434	38.0	33.0	40.0	15.0	41.0
39	33.811621315891045	38.0	33.0	40.0	15.0	41.0
40	33.728295681308666	38.0	33.0	40.0	15.0	41.0
41	33.62411977614085	38.0	33.0	40.0	13.0	41.0
42	33.47234044082021	38.0	33.0	40.0	12.0	41.0
43	33.3635765933335	38.0	33.0	40.0	12.0	41.0
44	33.21177724126038	38.0	32.0	40.0	10.0	41.0
45	33.26134539265683	38.0	33.0	40.0	10.0	41.0
46	33.17509782546213	38.0	32.0	40.0	10.0	41.0
47	32.96436504808126	37.0	32.0	40.0	10.0	41.0
48	32.80950467262858	37.0	31.0	40.0	10.0	41.0
49	32.77832729751258	37.0	31.0	40.0	10.0	41.0
50	32.65081185895067	37.0	31.0	40.0	10.0	41.0
51	31.842698093994564	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	7.0
12	9.0
13	10.0
14	19.0
15	51.0
16	131.0
17	348.0
18	802.0
19	1699.0
20	3045.0
21	5005.0
22	7904.0
23	12878.0
24	22692.0
25	40468.0
26	57616.0
27	57816.0
28	49103.0
29	44053.0
30	43741.0
31	48561.0
32	57074.0
33	71937.0
34	101499.0
35	132771.0
36	161740.0
37	234120.0
38	388722.0
39	404437.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.54399784845573	3.806621747021097	52.41160807403941	24.23777233048377
2	31.30933170735713	3.463360104456653	46.195636553258936	19.03167163492728
3	15.742559927077432	3.2925504832762598	60.88690637497639	20.07798321466992
4	14.520100925492516	3.5316736879275377	60.61483251623924	21.333392870340717
5	14.680645545400047	4.371145492022041	60.985501712202215	19.9627072503757
6	17.344362050700894	4.8456965008663655	61.88759002406117	15.922351424371575
7	71.47540916295073	1.0932226355596069	22.922466392385832	4.508901809103824
8	74.06049575850147	1.1251981145245662	20.67253208839398	4.141774038579981
9	69.51889992034359	3.5523576654923508	22.387659826069818	4.541082588094241
10	30.488490880572872	27.04596872870012	29.582399218217503	12.883141172509504
11	18.948884399661665	22.825000205300025	40.44585006528541	17.7802653297529
12	16.757614577944207	19.91179284406231	43.878620465948934	19.45197211204454
13	17.860537639706667	20.54344969738776	44.62401353337768	16.971999129527894
14	17.17699120494691	22.5998887273862	42.67232884136878	17.550791226298113
15	15.261387992412113	24.622247953158745	42.67135366624785	17.445010388181288
16	17.03630936250236	24.56158179563614	40.79142133313625	17.61068750872525
17	17.69065186864083	24.92716981596906	38.93597102806041	18.446207287329702
18	17.946814975405058	23.990591099833296	40.03032281376003	18.03227111100162
19	17.601038407528762	26.568184244454844	38.199970436796335	17.630806911220056
20	18.451391112972498	26.379000270996034	37.711818301265474	17.457790314766
21	18.742095949019898	26.032453828024277	38.83445016547182	16.391000057484007
22	18.380562604189763	23.792373925254367	38.15696008146305	19.67010338909282
23	17.007105433881073	25.01863097731024	37.67075829617403	20.303505292634657
24	17.5597217774055	24.650733331690933	38.345682129864585	19.443862761038982
25	17.17267990441231	25.794408448506644	36.765949758977776	20.266961888103275
26	16.76392755372702	26.755212567646357	36.99049666182159	19.49036321680504
27	17.201473232982682	26.167797869806936	37.298600675026485	19.3321282221839
28	16.244569814326656	25.223417752703803	38.13001445312179	20.40199797984775
29	16.56884120453631	23.921251016235125	37.76006380724791	21.749843971980653
30	18.083134192308638	25.4685459830997	36.198551813620426	20.249768010971234
31	17.080756818013846	27.61069777372652	34.864717548224974	20.443827860034656
32	16.805295508856645	28.081861332150805	34.65428502213134	20.45855813686121
33	18.331136623060942	27.406321598383876	33.745113859394124	20.51742791916106
34	17.42653338589014	26.772098494740217	33.563474661870856	22.23789345749879
35	17.266963941103526	26.519630788434217	33.03616154648403	23.177243723978222
36	18.93199847256781	28.395097845992133	31.758579488063855	20.9143241933762
37	18.19271308089642	29.239958775754886	32.52255220779647	20.044775935552217
38	18.61404005814097	28.181380519491185	33.59262726548578	19.61195215688207
39	20.862999187011898	25.714341438578337	32.6999314297915	20.722727944618267
40	20.807311555106633	25.35193556864001	32.10635773118835	21.734395145064997
41	19.207459781725014	25.986363972309135	31.982048565774022	22.824127680191832
42	19.978669327354996	27.254707529583733	31.352957962766787	21.413665180294483
43	19.463622888489237	27.117002537508316	32.224610545851704	21.19476402815074
44	18.87353929031887	27.250755504093682	30.62599057262283	23.24971463296461
45	19.60887265650021	25.890745485452438	30.27780172944741	24.222580128599937
46	19.700385142847757	26.28558875941301	30.395746594072577	23.61827950366666
47	17.775132829116473	27.183879020801	31.664500751398094	23.376487398684436
48	17.765740352951802	26.73601701526611	32.41343524426597	23.084807387516115
49	19.645929311095234	25.117944864625162	32.39429101689208	22.841834807387514
50	18.97387967776108	24.411199526988742	31.941655785765317	24.67326500948486
51	18.182602054642654	24.045919456694012	31.412597620162103	26.358880868501227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	648.0
2	1081.0
3	30518.0
4	59955.0
5	42067.5
6	24180.0
7	24308.0
8	24436.0
9	25600.0
10	26764.0
11	27349.5
12	27935.0
13	28069.0
14	28203.0
15	27470.0
16	26737.0
17	25874.0
18	25011.0
19	23872.0
20	22733.0
21	22020.0
22	21307.0
23	20526.0
24	19745.0
25	20274.0
26	21936.0
27	23069.0
28	24426.0
29	25783.0
30	29661.0
31	33539.0
32	36144.5
33	38750.0
34	43723.0
35	48696.0
36	56270.0
37	63844.0
38	68323.5
39	72803.0
40	82414.5
41	92026.0
42	103122.5
43	114219.0
44	131962.5
45	149706.0
46	177130.0
47	204554.0
48	214921.0
49	225288.0
50	205206.5
51	185125.0
52	152844.5
53	120564.0
54	98857.0
55	77150.0
56	63797.5
57	50445.0
58	43042.0
59	35639.0
60	29739.5
61	23840.0
62	19725.0
63	15610.0
64	12092.5
65	8575.0
66	6632.5
67	4690.0
68	3516.5
69	2343.0
70	1793.0
71	1243.0
72	1008.5
73	774.0
74	621.5
75	393.5
76	318.0
77	222.0
78	126.0
79	79.5
80	33.0
81	25.0
82	17.0
83	19.0
84	21.0
85	12.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1948368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.5768918224582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.247307222399	32.88950243184277
2	10.235393202676283	8.715824583013443
3	3.9121842746798245	4.9970593995769494
4	2.016976823400489	3.4350641607331194
5	1.2181918016604816	2.593341027915189
6	0.8282933666735222	2.115969424207096
7	0.6172058468541819	1.8395094601589441
8	0.4629173851487006	1.5767666744169158
9	0.3760789048313619	1.4411043762942106
>10	2.8424284424750046	23.74932523547343
>50	0.1723804933888118	4.780134201998821
>100	0.06370024529641159	4.893681687801834
>500	0.004019047140824879	1.1465978950097866
>1k	0.0023139968386567487	2.5727412518501516
>5k	4.8715722919089445E-4	1.2323127249867754
>10k+	1.2178930729772361E-4	2.0210654647205786
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38976	2.0004434480549875	No Hit
CTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGCT	6255	0.3210379148087014	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCT	6157	0.316008064185	No Hit
TCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	5874	0.30148308738390284	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTT	5479	0.28120970987000404	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCC	4942	0.2536481814523745	No Hit
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	4434	0.22757507821930972	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC	4153	0.21315275143094117	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGT	3968	0.20365762525354553	No Hit
CCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	3801	0.1950863491907073	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTG	3496	0.17943222224959557	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT	3347	0.17178479630131474	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTC	3282	0.16844867088763518	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG	2770	0.14217026762911317	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC	2320	0.11907401476517782	No Hit
TCCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTG	2308	0.11845811468880621	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT	2298	0.11794486462516321	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0530002545720316E-4	0.0	0.0	0.3497285933663456	0.0
2	2.0530002545720316E-4	0.0	0.0	1.1630246442150558	0.0
3	2.0530002545720316E-4	0.0	0.0	1.837537877854697	0.0
4	2.0530002545720316E-4	0.0	0.0	2.7633383426539546	0.0
5	2.0530002545720316E-4	0.0	0.0	4.268957404350719	0.0
6	2.0530002545720316E-4	0.0	0.0	5.993580268203953	0.0
7	2.0530002545720316E-4	0.0	0.0	7.503715930460776	0.0
8	2.5662503182150395E-4	0.0	0.0	9.281768125939248	0.0
9	2.5662503182150395E-4	0.0	0.0	10.440994719683346	0.0
10	2.5662503182150395E-4	0.0	0.0	11.924082103586182	0.0
11	2.5662503182150395E-4	0.0	0.0	14.081682721128658	0.0
12	2.5662503182150395E-4	0.0	0.0	15.781977531965214	0.0
13	2.5662503182150395E-4	0.0	0.0	16.500168346020875	0.0
14	2.5662503182150395E-4	0.0	0.0	16.78676718155913	0.0
15	2.5662503182150395E-4	0.0	0.0	17.084195593440253	0.0
16	2.5662503182150395E-4	0.0	0.0	17.694244619086334	0.0
17	2.5662503182150395E-4	0.0	0.0	18.668290589868032	0.0
18	2.5662503182150395E-4	0.0	0.0	19.719324070196183	0.0
19	3.0795003818580476E-4	0.0	0.0	20.493202516157112	0.0
20	3.592750445501055E-4	0.0	0.0	21.234951508133985	0.0
21	3.592750445501055E-4	0.0	0.0	22.271973261724685	0.0
22	3.592750445501055E-4	0.0	0.0	23.39096105047917	0.0
23	3.592750445501055E-4	0.0	0.0	24.5372024176131	0.0
24	3.592750445501055E-4	0.0	0.0	25.3637403201038	0.0
25	3.592750445501055E-4	0.0	0.0	26.08968121012047	0.0
26	3.592750445501055E-4	0.0	0.0	26.73350208995426	0.0
27	3.592750445501055E-4	0.0	0.0	27.390051571366396	0.0
28	3.592750445501055E-4	0.0	0.0	28.09125380831547	0.0
29	3.592750445501055E-4	0.0	0.0	28.830949800037775	0.0
30	3.592750445501055E-4	0.0	0.0	29.619609847831622	0.0
31	3.592750445501055E-4	0.0	0.0	30.355251164051143	0.0
32	3.592750445501055E-4	1.5397501909290238E-4	0.0	31.04593177469554	0.0
33	3.592750445501055E-4	1.5397501909290238E-4	0.0	31.734456735072634	0.0
34	3.592750445501055E-4	1.5397501909290238E-4	0.0	32.393110541745706	0.0
35	3.592750445501055E-4	1.5397501909290238E-4	0.0	33.09272170349749	0.0
36	3.592750445501055E-4	1.5397501909290238E-4	0.0	33.77539561314906	0.0
37	3.592750445501055E-4	1.5397501909290238E-4	0.0	34.449446921731415	0.0
38	3.592750445501055E-4	1.5397501909290238E-4	0.0	35.11477297923185	0.0
39	3.592750445501055E-4	1.5397501909290238E-4	0.0	35.77732748638861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	25	3.891807E-5	45.000004	1
CCGCGCA	25	3.891807E-5	45.000004	42
GCCCGCG	25	3.891807E-5	45.000004	1
CGGCGCC	35	1.212502E-7	45.0	42
CCGCTCG	20	7.0344965E-4	45.0	1
GTCCGCG	40	6.8193913E-9	45.0	1
CAGTACG	20	7.0344965E-4	45.0	22
AGTACGT	20	7.0344965E-4	45.0	23
CCGTGCG	30	2.1662818E-6	44.999996	1
CGTTTTT	30110	0.0	44.58901	1
TATCGCG	105	0.0	42.857143	1
CTTCGCG	90	0.0	42.5	1
ACGGGGC	125	0.0	41.399998	5
TTTCGCG	560	0.0	40.98214	1
TTCGTAG	100	0.0	40.500004	1
TTCCGCG	190	0.0	40.26316	1
GGCGCGA	45	1.929402E-8	40.0	8
TTCTCCG	295	0.0	39.661015	1
GTTTTTT	33945	0.0	39.452053	2
GCGCGAC	35	6.250526E-6	38.571426	9
>>END_MODULE