##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801476
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988921689482904	33.0	31.0	34.0	30.0	34.0
2	32.17785186331219	33.0	31.0	34.0	30.0	34.0
3	32.25230325050282	34.0	31.0	34.0	30.0	34.0
4	35.80278885456333	37.0	35.0	37.0	35.0	37.0
5	35.77441620210711	37.0	35.0	37.0	35.0	37.0
6	35.449731495391006	37.0	35.0	37.0	33.0	37.0
7	35.82436404833083	37.0	35.0	37.0	35.0	37.0
8	35.932791499683084	37.0	35.0	37.0	35.0	37.0
9	37.7214414405422	39.0	38.0	39.0	35.0	39.0
10	37.079606875315044	39.0	37.0	39.0	33.0	39.0
11	36.9250869645504	39.0	37.0	39.0	33.0	39.0
12	36.662105914587585	39.0	35.0	39.0	33.0	39.0
13	36.47545902809317	39.0	35.0	39.0	32.0	39.0
14	37.21274748089775	39.0	36.0	41.0	31.0	41.0
15	37.36420429307927	39.0	36.0	41.0	32.0	41.0
16	37.55031841252888	39.0	36.0	41.0	33.0	41.0
17	37.57095408970449	39.0	36.0	41.0	33.0	41.0
18	37.59659553124485	39.0	36.0	41.0	33.0	41.0
19	37.60077656723345	39.0	36.0	41.0	33.0	41.0
20	37.52394082917018	39.0	36.0	41.0	33.0	41.0
21	37.35231123577	39.0	35.0	41.0	32.0	41.0
22	37.512357200964225	39.0	35.0	41.0	33.0	41.0
23	37.4868779601635	39.0	35.0	41.0	33.0	41.0
24	37.44421043175341	39.0	35.0	41.0	33.0	41.0
25	37.366782037141476	39.0	35.0	41.0	32.0	41.0
26	37.31783359701351	39.0	35.0	41.0	32.0	41.0
27	37.263369084039944	39.0	35.0	40.0	32.0	41.0
28	37.200584920821086	39.0	35.0	40.0	32.0	41.0
29	37.108281470686585	39.0	35.0	40.0	32.0	41.0
30	36.99872984343885	39.0	35.0	40.0	31.0	41.0
31	36.853308146469764	39.0	35.0	40.0	31.0	41.0
32	36.7249125363704	39.0	35.0	40.0	31.0	41.0
33	36.701841352704264	39.0	35.0	40.0	31.0	41.0
34	36.577412174537976	39.0	35.0	40.0	30.0	41.0
35	36.48722107711273	38.0	35.0	40.0	30.0	41.0
36	36.28081564513472	38.0	35.0	40.0	30.0	41.0
37	36.228005579705446	38.0	35.0	40.0	30.0	41.0
38	36.11387240541202	38.0	35.0	40.0	30.0	41.0
39	36.01644840269702	38.0	35.0	40.0	30.0	41.0
40	35.8526244079673	38.0	35.0	40.0	29.0	41.0
41	35.744500147228365	38.0	35.0	40.0	29.0	41.0
42	35.77850865153791	38.0	35.0	40.0	29.0	41.0
43	35.797935309354244	38.0	35.0	40.0	29.0	41.0
44	35.66073968528066	38.0	34.0	40.0	29.0	41.0
45	35.71420479215847	38.0	34.0	40.0	29.0	41.0
46	35.573746437822216	38.0	34.0	40.0	28.0	41.0
47	35.31395699933623	38.0	34.0	40.0	27.0	41.0
48	35.19417923930349	37.0	34.0	40.0	27.0	41.0
49	35.21743258687721	37.0	34.0	40.0	27.0	41.0
50	35.06451097724698	37.0	34.0	40.0	27.0	41.0
51	33.87066736870474	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	11.0
11	11.0
12	5.0
13	9.0
14	15.0
15	20.0
16	33.0
17	80.0
18	162.0
19	319.0
20	495.0
21	856.0
22	1407.0
23	2069.0
24	3118.0
25	4808.0
26	6551.0
27	8353.0
28	10091.0
29	12765.0
30	16189.0
31	21456.0
32	28605.0
33	40117.0
34	63925.0
35	68408.0
36	74340.0
37	109749.0
38	173905.0
39	153569.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.193957149059983	4.773817307068459	59.06090762543108	12.971317918440478
2	20.175650924045136	4.000993167605768	54.37592641576292	21.447429492586178
3	20.417704335501	3.6439020008085086	53.808223827039114	22.13016983665138
4	19.0988875524657	4.291207721753365	52.076419006932205	24.533485718848723
5	16.832194600961227	5.054549356437374	53.91852032999117	24.194735712610235
6	20.351576341649658	5.845964196058272	55.993067789927586	17.809391672364487
7	81.40755306459582	1.4971128268344904	11.276569728850271	5.8187643797194175
8	83.66775798651489	1.7454047282763303	8.758715170510408	5.828122114698382
9	79.24454381665826	3.9198927977880813	11.2435057319246	5.5920576536290545
10	41.33186770408596	21.33126880904731	21.039806556902516	16.297056929964214
11	29.71380303340337	25.034186925123148	25.314794204692344	19.93721583678114
12	25.681617415867724	21.96634708961965	32.23490160653594	20.11713388797668
13	24.95171408750855	24.026670792388042	33.491458259511205	17.530156860592207
14	20.535362256636507	25.610623399827322	35.03822946663406	18.815784876902118
15	18.10596948629778	25.06001427366509	37.33037545728132	19.503640782755816
16	22.0989773867215	23.54468505607155	31.87169671955243	22.484640837654528
17	22.14339543542165	24.274089305231847	28.49941857273331	25.0830966866132
18	23.786738467527414	22.619142681752166	32.10127315103634	21.492845699684082
19	23.31348661719128	26.33516162679856	29.805259296597775	20.546092459412385
20	23.049598490784504	28.75943883534878	29.261262969820677	18.929699704046037
21	22.366982916519024	27.177233005105577	31.655470656638503	18.800313421736895
22	20.73549301538661	23.91911922502982	31.099995508287208	24.24539225129636
23	19.553049623444743	25.18141528879218	30.442583433565073	24.822951654198004
24	21.218975989299743	26.301598550674004	28.915650624597617	23.56377483542863
25	21.400516047891642	25.756104986300276	27.913125283851294	24.930253681956792
26	19.095518767873273	26.47141024809227	29.271369323597963	25.1617016604365
27	20.631684542019972	25.175052029006483	31.401189804810127	22.792073624163418
28	18.541041777919737	23.8774461119235	30.666545224061608	26.914966886095154
29	20.660132056356023	24.021305691000105	31.116589891649905	24.201972360993967
30	24.974546960857218	23.451606785480788	30.056545673232886	21.517300580429108
31	23.741197490629787	25.358588404393895	28.76792318172971	22.132290923246607
32	24.403725127140426	24.818335171608382	29.06674685205795	21.71119284919324
33	24.629184155233595	23.07555060912616	30.29535506989604	21.9999101657442
34	20.702803327860096	22.777725097195674	31.975754732518503	24.543716842425724
35	20.729254525400638	23.937335615788868	30.80541401114943	24.527995847661067
36	25.26925322779472	22.575598021650055	30.90809955631859	21.247049194236634
37	22.402417539639366	25.698586108629577	29.4378122364238	22.46118411530726
38	22.536545074337848	25.294082417938903	28.91752217159341	23.251850336129838
39	22.22349764684158	22.123307497666804	31.838757492426474	23.814437363065146
40	24.58376794813569	21.450673507378887	31.63026715709516	22.335291387390267
41	20.526628370656137	21.865657861246003	30.781208670003846	26.826505098094017
42	20.481087393758514	21.786054729024947	33.500316915291286	24.23254096192525
43	21.7524916529004	21.288971846942392	32.36466219824424	24.593874301912972
44	22.19430151370721	21.73165509634724	29.05975974327366	27.01428364667189
45	22.797937804750234	20.51876787327381	28.269842141249395	28.413452180726562
46	23.224775289590703	21.40588114927958	31.519970654143105	23.84937290698661
47	19.58748608816733	22.067410627392462	34.7003528489936	23.6447504354466
48	20.467612255388808	22.412773433016085	32.8975540128463	24.222060298748808
49	23.834525300819987	19.186475951868804	33.51628744965539	23.462711297655826
50	22.694004561583878	19.684931301748275	30.511331593210528	27.10973254345732
51	20.97679780804416	20.393249454755974	29.07510642863916	29.554846308560705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	345.5
2	600.0
3	1774.0
4	2948.0
5	2018.0
6	1088.0
7	1202.5
8	1317.0
9	1543.5
10	1770.0
11	1932.0
12	2094.0
13	2191.5
14	2289.0
15	2241.5
16	2194.0
17	2132.5
18	2071.0
19	2158.0
20	2245.0
21	2274.0
22	2303.0
23	2619.5
24	2936.0
25	3455.0
26	4407.0
27	4840.0
28	5711.5
29	6583.0
30	8003.5
31	9424.0
32	10526.0
33	11628.0
34	14506.0
35	17384.0
36	19290.5
37	21197.0
38	23798.0
39	26399.0
40	30314.0
41	34229.0
42	40601.0
43	46973.0
44	54508.5
45	62044.0
46	74587.5
47	87131.0
48	102557.5
49	117984.0
50	109675.0
51	101366.0
52	84031.5
53	66697.0
54	57263.5
55	47830.0
56	41564.5
57	35299.0
58	30236.0
59	25173.0
60	20484.0
61	15795.0
62	13678.5
63	11562.0
64	9692.0
65	7822.0
66	6729.0
67	5636.0
68	4734.0
69	3832.0
70	3047.5
71	2263.0
72	2056.5
73	1850.0
74	1540.5
75	918.0
76	605.0
77	532.5
78	460.0
79	327.0
80	194.0
81	133.0
82	72.0
83	50.0
84	28.0
85	20.0
86	12.0
87	9.0
88	6.0
89	3.5
90	1.0
91	2.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.36383749966581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.48917664239059	23.184255974822925
2	12.959129649776585	8.647325915444942
3	5.532130284110185	5.537192874780967
4	2.8514374106646403	3.805395776395316
5	1.6762047549034522	2.796231152938296
6	1.121828666129995	2.2457105591536806
7	0.7971316424660634	1.8616759409555612
8	0.5693459159646885	1.5196451697075402
9	0.45229198072432497	1.3581176532559525
>10	4.001062929474438	29.321576788705876
>50	0.45038414554249573	9.610323245267876
>100	0.09158438723832243	5.486259939893288
>500	0.0045226857895467685	1.0229366172594958
>1k	0.003015123859697846	2.1304997057440245
>5k	7.537809649244615E-4	1.4728526856743052
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	6135	0.7654627212792398	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	5578	0.6959659428354686	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4904	0.6118710978245138	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3355	0.4186026780589812	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	2162	0.2697523069935968	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1969	0.24567173564772993	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1283	0.16007965304014093	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1143	0.1426118810794085	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1064	0.1327550669015666	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	1063	0.1326302971018471	No Hit
TTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	966	0.1205276265290539	No Hit
TTTCTGGGGCAGAATATGAGAAATACACAATTTAGGACTTCAAATATGGTG	939	0.11715884193662693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.49539599439035E-4	0.0	0.0	0.8403246011109503	0.0
2	2.49539599439035E-4	0.0	0.0	2.8256616542479125	0.0
3	2.49539599439035E-4	0.0	0.0	3.9727951928691563	0.0
4	2.49539599439035E-4	0.0	0.0	5.1712091191751215	0.0
5	2.49539599439035E-4	0.0	0.0	7.014183830832115	0.0
6	2.49539599439035E-4	0.0	0.0	8.210476670542848	0.0
7	2.49539599439035E-4	0.0	0.0	9.229596394651868	0.0
8	2.49539599439035E-4	0.0	0.0	10.851978100404754	0.0
9	2.49539599439035E-4	0.0	0.0	11.590740084544016	0.0
10	2.49539599439035E-4	0.0	0.0	13.070634679017214	0.0
11	2.49539599439035E-4	0.0	0.0	16.006842375816618	0.0
12	2.49539599439035E-4	0.0	0.0	18.33542114798197	0.0
13	2.49539599439035E-4	0.0	0.0	19.103379265255604	0.0
14	2.49539599439035E-4	0.0	0.0	19.36502153526743	0.0
15	2.49539599439035E-4	0.0	0.0	19.719118226871423	0.0
16	2.49539599439035E-4	0.0	0.0	20.708792278246634	0.0
17	2.49539599439035E-4	0.0	0.0	22.26429737134986	0.0
18	2.49539599439035E-4	0.0	0.0	24.03053865617935	0.0
19	2.49539599439035E-4	0.0	0.0	25.184409763985446	0.0
20	2.49539599439035E-4	0.0	0.0	26.31869201323558	0.0
21	2.49539599439035E-4	0.0	0.0	27.933961840404454	0.0
22	2.49539599439035E-4	0.0	0.0	29.591653399477963	0.0
23	2.49539599439035E-4	0.0	0.0	31.292889618653586	0.0
24	2.49539599439035E-4	0.0	0.0	32.520873987493076	0.0
25	2.49539599439035E-4	0.0	0.0	33.55109822377713	0.0
26	2.49539599439035E-4	0.0	0.0	34.49860008284715	0.0
27	2.49539599439035E-4	0.0	0.0	35.53493803931746	0.0
28	2.49539599439035E-4	0.0	0.0	36.491298554167564	0.0
29	2.49539599439035E-4	0.0	0.0	37.56706376734924	0.0
30	2.49539599439035E-4	0.0	0.0	38.65243625510932	0.0
31	2.49539599439035E-4	0.0	0.0	39.70237411974907	0.0
32	2.49539599439035E-4	0.0	0.0	40.61069826170715	0.0
33	2.49539599439035E-4	0.0	0.0	41.54534883140606	0.0
34	2.49539599439035E-4	0.0	0.0	42.46677380233469	0.0
35	2.49539599439035E-4	0.0	0.0	43.537423453727875	0.0
36	2.49539599439035E-4	0.0	0.0	44.42640827672943	0.0
37	2.49539599439035E-4	0.0	0.0	45.32475083470996	0.0
38	2.49539599439035E-4	0.0	0.0	46.306938698102	0.0
39	2.49539599439035E-4	0.0	0.0	47.867933662392886	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	35	1.2113196E-7	45.000004	19
CTAACGT	35	1.2113196E-7	45.000004	30
AAACTCG	20	7.032007E-4	45.0	42
TTAGGCG	20	7.032007E-4	45.0	1
GACAGCG	25	3.8897448E-5	45.0	9
ATAGGCG	25	3.8897448E-5	45.0	12
TCCGATG	25	3.8897448E-5	45.0	19
CGTGCCC	20	7.032007E-4	45.0	16
CGGTTAC	20	7.032007E-4	45.0	39
GGCGTAA	25	3.8897448E-5	45.0	8
CCGTGCG	25	3.8897448E-5	45.0	38
GCGTTAG	25	3.8897448E-5	45.0	1
GCCGCTC	20	7.032007E-4	45.0	17
ATCGTTA	20	7.032007E-4	45.0	42
ATCGTAC	20	7.032007E-4	45.0	17
GTTACGG	20	7.032007E-4	45.0	2
ACAGCGG	25	3.8897448E-5	45.0	10
CATACGG	25	3.8897448E-5	45.0	3
CGTCGTG	40	6.8102963E-9	45.0	18
CCGGTTT	20	7.032007E-4	45.0	26
>>END_MODULE