FastQCFastQC Report
Sat 14 Jan 2017
SRR2934378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013549
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141280.7016466944683243No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT48360.2401729483613262Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC44380.22040685376914096TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC41510.2061534136988968TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC29430.146159840162817TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC21260.10558471633916036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA207.0345507E-445.00000440
CGTTTTT83100.043.1588441
TATCCGG950.040.2631572
CGCATCG403.4599543E-739.37500421
TTTCGCG5500.038.0454561
TGTTTCG3400.037.720591
GCCGATT2950.037.3728839
TGGGCGA27700.037.3646246
TTTGGGA89700.037.0484964
TTGGGAC31850.037.017275
TTTGTCG5200.036.7788431
GGCGATT20950.036.515518
TCAAGCG4650.036.29032517
GACACGA4600.036.19565625
AGGCACG3050.036.1475410
CGACCAA4550.036.098929
ACGTCGA250.002107483836.00000429
GTACGCA250.002107483836.00000413
CGTTAGG1250.036.02
GCGAGAC4700.035.90425521
Produced by FastQC (version 0.11.5)