##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2013549
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.080183298246034	33.0	31.0	34.0	30.0	34.0
2	32.28343338056337	34.0	31.0	34.0	30.0	34.0
3	32.32813703565198	34.0	31.0	34.0	30.0	34.0
4	35.88068976717229	37.0	35.0	37.0	35.0	37.0
5	35.844707032210295	37.0	35.0	37.0	35.0	37.0
6	35.50587941987009	37.0	35.0	37.0	33.0	37.0
7	35.838589475597566	37.0	35.0	37.0	35.0	37.0
8	35.930704939388114	37.0	35.0	37.0	35.0	37.0
9	37.705700730401894	39.0	38.0	39.0	35.0	39.0
10	37.15546828013622	39.0	37.0	39.0	34.0	39.0
11	37.02580518278919	39.0	37.0	39.0	33.0	39.0
12	36.73441123111481	39.0	35.0	39.0	33.0	39.0
13	36.62004003875744	39.0	35.0	39.0	33.0	39.0
14	37.56173204625266	40.0	36.0	41.0	32.0	41.0
15	37.639387469587284	40.0	36.0	41.0	32.0	41.0
16	37.735055864049	40.0	36.0	41.0	33.0	41.0
17	37.67749828784897	40.0	36.0	41.0	33.0	41.0
18	37.62157166277056	39.0	36.0	41.0	33.0	41.0
19	37.587664864376286	39.0	36.0	41.0	33.0	41.0
20	37.48256536096216	39.0	35.0	41.0	33.0	41.0
21	37.313927796145016	39.0	35.0	41.0	32.0	41.0
22	37.45344414265558	39.0	35.0	41.0	33.0	41.0
23	37.405122001004194	39.0	35.0	41.0	33.0	41.0
24	37.33161994071165	39.0	35.0	41.0	32.0	41.0
25	37.25318777938853	39.0	35.0	41.0	32.0	41.0
26	37.206020812009044	39.0	35.0	41.0	32.0	41.0
27	37.142250821807664	39.0	35.0	41.0	32.0	41.0
28	36.99228625675362	39.0	35.0	41.0	32.0	41.0
29	36.8887730072623	39.0	35.0	41.0	31.0	41.0
30	36.80608467933981	39.0	35.0	40.0	31.0	41.0
31	36.689746313598526	39.0	35.0	40.0	31.0	41.0
32	36.52431105475953	39.0	35.0	40.0	30.0	41.0
33	36.38333906947385	39.0	35.0	40.0	30.0	41.0
34	36.09831049554791	39.0	35.0	40.0	29.0	41.0
35	36.00686201329096	38.0	35.0	40.0	29.0	41.0
36	35.8327564911507	38.0	35.0	40.0	29.0	41.0
37	35.78575490340687	38.0	35.0	40.0	29.0	41.0
38	35.69519589540657	38.0	35.0	40.0	28.0	41.0
39	35.62327760585911	38.0	35.0	40.0	28.0	41.0
40	35.488001036975014	38.0	34.0	40.0	27.0	41.0
41	35.38335148536241	38.0	34.0	40.0	27.0	41.0
42	35.319810444146135	38.0	34.0	40.0	27.0	41.0
43	35.251277222456466	38.0	34.0	40.0	26.0	41.0
44	35.102564675605116	38.0	34.0	40.0	26.0	41.0
45	35.13030375719687	38.0	34.0	40.0	26.0	41.0
46	35.00667478169143	37.0	34.0	40.0	26.0	41.0
47	34.7822054491845	37.0	34.0	40.0	25.0	41.0
48	34.67202635744151	37.0	34.0	40.0	24.0	41.0
49	34.67791049534926	37.0	34.0	40.0	25.0	41.0
50	34.58333767889433	37.0	34.0	40.0	24.0	41.0
51	33.56550846291796	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	19.0
10	38.0
11	28.0
12	29.0
13	26.0
14	59.0
15	68.0
16	178.0
17	332.0
18	651.0
19	1196.0
20	2175.0
21	3397.0
22	5054.0
23	7795.0
24	11746.0
25	18696.0
26	25990.0
27	29543.0
28	31502.0
29	35572.0
30	42212.0
31	52023.0
32	66821.0
33	93301.0
34	153187.0
35	162528.0
36	178083.0
37	253576.0
38	403001.0
39	434566.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.718320239537256	4.641456453257407	54.729733420939844	16.910489886265495
2	22.234025593616046	4.2217497562761075	51.29217118629842	22.252053463809425
3	20.284631762127468	4.417175842256633	52.966230273015455	22.331962122600444
4	18.177506482335417	4.71595178463499	51.88902778129562	25.217513951733977
5	17.582090130411526	5.899930917996036	52.37404205211793	24.14393689947451
6	21.2778035200534	6.028807841279254	54.882101205384124	17.811287433283223
7	81.55202580120971	2.2679358684591238	11.667458800356982	4.51257952997419
8	83.61793033097283	2.4908755634951025	9.584916979919536	4.306277125612538
9	78.44368326770295	4.667877464119323	11.673418426867189	5.215020841310541
10	41.81800393236023	23.838953012814688	19.51742917604687	14.825613878778219
11	31.48247199348017	23.381452351047827	26.230352477143594	18.905723178328415
12	26.99035384785769	21.080688873228315	31.50382732180841	20.42512995710559
13	25.323595303615654	21.369432777647827	33.15881560369278	20.148156315043735
14	21.47953687742389	22.43570928743229	35.01896402819102	21.065789806952797
15	20.08826206861616	22.075002892902035	36.32893959868868	21.50779543979312
16	24.73319497067119	22.61628597069155	31.715791371354758	20.934727687282503
17	24.819112919526667	22.823879627463747	30.625527364866713	21.731480088142877
18	25.88101903653698	21.785017399626234	31.747973354509874	20.585990209326916
19	24.948834123232164	25.42431299163815	29.297871569055435	20.328981316074255
20	25.250589878865625	27.159905222073068	28.60516431435242	18.98434058470889
21	24.097551139803404	26.604915003310076	30.770544943281735	18.526988913604782
22	23.51628890084125	23.119477102369995	31.566105418840067	21.798128577948685
23	22.923306063075692	24.054343847604404	30.588329362732168	22.43402072658773
24	23.374797434778095	24.40273368068023	30.571642408503592	21.65082647603808
25	22.985037861010586	25.02397508081502	27.575787825376985	24.415199232797413
26	20.976296082191194	24.503848677136737	29.64268562622514	24.877169614446927
27	20.755243602216783	23.7456351943757	31.576683755895687	23.922437447511832
28	18.964971798550717	25.679285679166487	30.36449572371966	24.991246798563136
29	21.02342679517608	24.820851143925477	29.976573701459465	24.179148359438983
30	23.007336796869605	24.079821250935538	31.123007187806206	21.78983476438865
31	22.966761673045948	24.805803086987204	29.62341616717547	22.604019072791377
32	23.985410834303014	24.37442545475675	28.33424962590928	23.305914085030956
33	23.607868494881426	24.377206613794847	28.09566591128401	23.91925898003972
34	20.367520234173593	25.006791491043924	30.486866721395906	24.138821553386585
35	19.957199948945867	26.29531240610484	28.93731416518793	24.810173479761357
36	21.60007032359282	27.72284160951633	27.27681322878162	23.40027483810923
37	20.548245908095605	28.20105197340616	28.16494656946516	23.085755549033077
38	22.008404066650478	27.87391814155007	27.890605095778646	22.22707269602081
39	21.308346605918207	25.27244184273638	27.323099661344223	26.09611189000119
40	23.194816714169857	23.759888634445947	27.417510077976747	25.627784573407453
41	21.254660303772095	23.755865886551554	26.77953206005913	28.20994174961722
42	21.414576948462642	23.555970080688375	28.222854273722664	26.806598697126315
43	20.492175755345414	23.178974040363556	29.518278422824572	26.810571781466457
44	20.897480021593715	23.896662062855185	28.326750429217267	26.87910748633383
45	21.334221317683355	23.060923771907216	27.58159846122443	28.023256449185
46	20.961744660795443	23.932022513482412	28.626320988463654	26.47991183725849
47	19.046618681740547	25.205048399616796	30.69505634081912	25.053276577823535
48	19.40230905729138	25.05600807330738	30.658702619106858	24.88298025029438
49	21.872574245772018	23.20877217291459	30.112502849446425	24.806150731866968
50	20.476084763767854	23.148430954498746	28.87434077839675	27.501143503336646
51	19.680871933089286	23.41765708209733	27.669254634478722	29.232216350334657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	1058.0
2	1849.0
3	12260.0
4	22671.0
5	15050.0
6	7429.0
7	7355.0
8	7281.0
9	7628.5
10	7976.0
11	8142.0
12	8308.0
13	8184.0
14	8060.0
15	7892.5
16	7725.0
17	7368.5
18	7012.0
19	7239.5
20	7467.0
21	7368.5
22	7270.0
23	7848.5
24	8427.0
25	9500.5
26	12934.5
27	15295.0
28	16840.5
29	18386.0
30	21794.0
31	25202.0
32	30615.5
33	36029.0
34	42037.0
35	48045.0
36	52127.5
37	56210.0
38	61794.5
39	67379.0
40	74756.5
41	82134.0
42	91065.5
43	99997.0
44	113376.0
45	126755.0
46	146151.0
47	165547.0
48	186146.5
49	206746.0
50	200673.5
51	194601.0
52	174234.5
53	153868.0
54	139966.0
55	126064.0
56	117669.5
57	109275.0
58	101297.5
59	93320.0
60	86441.5
61	79563.0
62	70665.5
63	61768.0
64	53336.0
65	44904.0
66	37895.5
67	30887.0
68	26419.0
69	21951.0
70	18407.0
71	14863.0
72	12211.0
73	9559.0
74	7598.5
75	4713.5
76	3789.0
77	2696.0
78	1603.0
79	1236.0
80	869.0
81	772.5
82	676.0
83	441.5
84	207.0
85	128.5
86	50.0
87	37.0
88	24.0
89	19.5
90	15.0
91	11.5
92	8.0
93	7.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2013549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.629894620950854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78157808202529	23.33703539701917
2	10.910447406033828	6.901926034405531
3	4.131448844217467	3.920318747233431
4	2.089156357646392	2.643191817561796
5	1.253915611088687	1.9830609321150185
6	0.8363985238698308	1.5873118302672957
7	0.6408843283307022	1.4189772638523284
8	0.4864358247108907	1.2308731100368637
9	0.37513627439196856	1.0678968744762662
>10	4.807658102268373	36.32293687970906
>50	0.6011566153059518	12.047849202094964
>100	0.07957713515923688	4.503461633415643
>500	0.004137929967576285	0.9591154803656051
>1k	0.0019098138311890549	1.3648505757178737
>5k	0.0	0.0
>10k+	1.5915115259908792E-4	0.711194221729138
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14128	0.7016466944683243	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	4836	0.2401729483613262	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	4438	0.22040685376914096	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	4151	0.2061534136988968	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2943	0.146159840162817	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	2126	0.10558471633916036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4831777125860853E-4	0.0	0.0	0.2650543890414388	0.0
2	2.4831777125860853E-4	0.0	0.0	0.9143060337741967	0.0
3	2.4831777125860853E-4	0.0	0.0	1.3517922831776132	0.0
4	2.4831777125860853E-4	0.0	0.0	1.9460663733537154	0.0
5	2.4831777125860853E-4	0.0	0.0	2.9365066357958014	0.0
6	2.4831777125860853E-4	0.0	0.0	3.8333807620276437	0.0
7	2.4831777125860853E-4	0.0	0.0	4.598497478829668	0.0
8	2.4831777125860853E-4	0.0	0.0	5.773487508871153	0.0
9	2.4831777125860853E-4	0.0	0.0	6.473048334060905	0.0
10	2.4831777125860853E-4	0.0	0.0	7.577317462847937	0.0
11	2.4831777125860853E-4	0.0	0.0	9.373747547241214	0.0
12	2.4831777125860853E-4	0.0	0.0	10.805945124752364	0.0
13	2.4831777125860853E-4	0.0	0.0	11.314152275410233	0.0
14	2.4831777125860853E-4	0.0	0.0	11.527606231584134	0.0
15	2.4831777125860853E-4	0.0	0.0	11.781138676039173	0.0
16	2.4831777125860853E-4	0.0	0.0	12.3429824652889	0.0
17	2.4831777125860853E-4	0.0	0.0	13.147283726395534	0.0
18	2.4831777125860853E-4	0.0	0.0	14.006413551396067	0.0
19	2.979813255103303E-4	0.0	0.0	14.660830205771004	0.0
20	3.47644879762052E-4	0.0	0.0	15.338141758655985	0.0
21	3.47644879762052E-4	0.0	0.0	16.317507048499937	0.0
22	3.47644879762052E-4	0.0	0.0	17.347678154343402	0.0
23	3.47644879762052E-4	0.0	0.0	18.348200118298585	0.0
24	3.47644879762052E-4	0.0	0.0	19.10636393750537	0.0
25	3.973084340137737E-4	0.0	0.0	19.788343864490013	0.0
26	3.973084340137737E-4	0.0	0.0	20.391656721539928	0.0
27	4.469719882654954E-4	0.0	0.0	21.030727337651083	0.0
28	4.469719882654954E-4	0.0	0.0	21.682760141421937	0.0
29	4.469719882654954E-4	0.0	0.0	22.39965354704554	0.0
30	4.469719882654954E-4	0.0	0.0	23.16114482438719	0.0
31	4.966355425172171E-4	0.0	0.0	23.835625554679822	0.0
32	4.966355425172171E-4	0.0	0.0	24.52038664070256	0.0
33	5.462990967689389E-4	0.0	0.0	25.206836287569857	0.0
34	5.959626510206606E-4	0.0	0.0	25.87580436334055	0.0
35	5.959626510206606E-4	0.0	0.0	26.62403547169699	0.0
36	5.959626510206606E-4	0.0	0.0	27.279594387819714	0.0
37	6.456262052723823E-4	0.0	0.0	27.971358034991948	0.0
38	6.456262052723823E-4	0.0	0.0	28.73473652739516	0.0
39	6.456262052723823E-4	0.0	0.0	29.895522780920654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	20	7.0345507E-4	45.000004	40
CGTTTTT	8310	0.0	43.158844	1
TATCCGG	95	0.0	40.263157	2
CGCATCG	40	3.4599543E-7	39.375004	21
TTTCGCG	550	0.0	38.045456	1
TGTTTCG	340	0.0	37.72059	1
GCCGATT	295	0.0	37.372883	9
TGGGCGA	2770	0.0	37.364624	6
TTTGGGA	8970	0.0	37.048496	4
TTGGGAC	3185	0.0	37.01727	5
TTTGTCG	520	0.0	36.778843	1
GGCGATT	2095	0.0	36.51551	8
TCAAGCG	465	0.0	36.290325	17
GACACGA	460	0.0	36.195656	25
AGGCACG	305	0.0	36.14754	10
CGACCAA	455	0.0	36.0989	29
ACGTCGA	25	0.0021074838	36.000004	29
GTACGCA	25	0.0021074838	36.000004	13
CGTTAGG	125	0.0	36.0	2
GCGAGAC	470	0.0	35.904255	21
>>END_MODULE