##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1645321
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19871563056692	33.0	31.0	34.0	30.0	34.0
2	32.39708786309784	34.0	31.0	34.0	30.0	34.0
3	32.4543490297638	34.0	31.0	34.0	30.0	34.0
4	35.94154818421451	37.0	35.0	37.0	35.0	37.0
5	35.893454225649585	37.0	35.0	37.0	35.0	37.0
6	35.52269921796415	37.0	35.0	37.0	33.0	37.0
7	35.844834533808296	37.0	35.0	37.0	35.0	37.0
8	35.93857186530774	37.0	35.0	37.0	35.0	37.0
9	37.721280528237344	39.0	38.0	39.0	35.0	39.0
10	37.25210825121663	39.0	37.0	39.0	34.0	39.0
11	37.10409579650415	39.0	37.0	39.0	33.0	39.0
12	36.76251138835522	39.0	35.0	39.0	33.0	39.0
13	36.586655734656034	39.0	35.0	39.0	33.0	39.0
14	37.350397885883666	40.0	36.0	41.0	32.0	41.0
15	37.48493819747028	40.0	36.0	41.0	32.0	41.0
16	37.68152840691877	40.0	36.0	41.0	33.0	41.0
17	37.70952719864391	40.0	36.0	41.0	33.0	41.0
18	37.69379166740107	39.0	36.0	41.0	33.0	41.0
19	37.723809518021106	40.0	36.0	41.0	33.0	41.0
20	37.625597071939154	39.0	35.0	41.0	33.0	41.0
21	37.457954405249794	39.0	35.0	41.0	32.0	41.0
22	37.610494243980355	39.0	35.0	41.0	33.0	41.0
23	37.593309755360806	39.0	35.0	41.0	33.0	41.0
24	37.523909923960126	39.0	35.0	41.0	33.0	41.0
25	37.440932802778306	39.0	35.0	41.0	33.0	41.0
26	37.373302230993225	39.0	35.0	41.0	33.0	41.0
27	37.28381027167343	39.0	35.0	41.0	32.0	41.0
28	37.17212325132907	39.0	35.0	41.0	32.0	41.0
29	37.065719090682	39.0	35.0	41.0	32.0	41.0
30	37.01296707451008	39.0	35.0	41.0	32.0	41.0
31	36.91562619087704	39.0	35.0	41.0	31.0	41.0
32	36.81235576522757	39.0	35.0	41.0	31.0	41.0
33	36.738050508077144	39.0	35.0	41.0	31.0	41.0
34	36.52510239643206	39.0	35.0	41.0	30.0	41.0
35	36.4386530044897	39.0	35.0	41.0	30.0	41.0
36	36.2699339520981	38.0	35.0	40.0	30.0	41.0
37	36.20277380523314	38.0	35.0	40.0	30.0	41.0
38	36.1222873834346	38.0	35.0	40.0	30.0	41.0
39	36.05004920012569	38.0	35.0	40.0	30.0	41.0
40	35.92102452955989	38.0	35.0	40.0	29.0	41.0
41	35.82505480693433	38.0	35.0	40.0	29.0	41.0
42	35.73505291672567	38.0	34.0	40.0	29.0	41.0
43	35.66274058375235	38.0	34.0	40.0	29.0	41.0
44	35.52136938627781	38.0	34.0	40.0	28.0	41.0
45	35.57590342553216	38.0	34.0	40.0	29.0	41.0
46	35.51354842003475	37.0	34.0	40.0	29.0	41.0
47	35.30366840270075	37.0	34.0	40.0	28.0	41.0
48	35.18809824952091	37.0	34.0	40.0	28.0	41.0
49	35.18248050076551	37.0	34.0	40.0	28.0	41.0
50	35.047633258190956	37.0	34.0	40.0	27.0	41.0
51	34.037476577518916	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	31.0
10	51.0
11	42.0
12	41.0
13	35.0
14	44.0
15	74.0
16	147.0
17	240.0
18	473.0
19	903.0
20	1494.0
21	2291.0
22	3499.0
23	5210.0
24	7630.0
25	10886.0
26	14885.0
27	18210.0
28	21956.0
29	26091.0
30	32577.0
31	41335.0
32	54166.0
33	78599.0
34	134134.0
35	129534.0
36	140425.0
37	206245.0
38	325644.0
39	388306.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.445650423230482	4.914542511765181	55.66409229566753	16.975714769336804
2	20.805544936216094	4.744849181405939	52.28803376362424	22.161572118753725
3	20.946125406531614	4.702486627229581	51.298074965310725	23.053313000928085
4	19.972637558263706	4.998052051848849	49.54771743629359	25.481592953593857
5	18.597282840248194	6.233737975750628	49.48699980125459	25.68197938274659
6	22.785523311256586	6.503654909892963	52.12429671778334	18.58652506106711
7	83.12572440271533	2.8593204608705536	9.227257173524194	4.787697962889916
8	85.20957308634607	3.0367326497382576	7.475076292103486	4.278617971812188
9	79.64160185155359	5.125808276925901	9.554245037898381	5.678344833622132
10	45.19671237405953	22.19056342197055	17.12413565498769	15.488588548982236
11	32.8404001407628	23.098653697363613	24.128969362209563	19.931976799664017
12	29.33336412772948	20.253069157933314	29.415111093823025	20.99845562051417
13	27.890423813954847	20.004850117393506	31.609151041043056	20.495575027608595
14	22.204664013891513	22.352355558580967	34.7769219501848	20.66605847734272
15	19.51193718429413	22.201199644324724	37.14849564309943	21.138367528281716
16	25.362224149573244	21.728769036558823	31.313646394837242	21.595360419030694
17	26.564299610835818	21.86412256331743	28.522640870687237	23.048936955159512
18	26.963188338324258	21.604355624221657	29.854478244670794	21.577977792783294
19	27.195118764058808	22.806978091205302	28.543609423328338	21.454293721407556
20	25.66508298380681	27.273097468518305	27.668704161680303	19.393115385994587
21	25.597375831220777	23.94936915045757	31.50060079461698	18.952654223704677
22	23.783322524905472	21.661061883972792	31.85007667196857	22.705538919153163
23	23.231576087584124	23.24142219056342	30.911718746676183	22.615282975176275
24	23.35690117612308	25.23817540771679	28.16137398112587	23.243549435034257
25	25.469133378836105	23.918493716423725	26.421591896049463	24.190781008690706
26	22.475310288995278	24.25544924060411	27.263616036019716	26.00562443438089
27	21.38300064242783	23.812374606535748	31.808808129234357	22.995816621802067
28	21.089744797519757	22.74291764342642	30.078325141416173	26.08901241763765
29	23.421812521690295	24.256178581565543	28.954228384613096	23.367780512131066
30	25.51593275719449	22.537243492303325	29.48786285472561	22.45896089577657
31	24.470969494706505	23.68328125636274	29.62321638148422	22.222532867446535
32	27.41939110969835	24.004677506699302	26.588003192082276	21.987928191520073
33	27.1924445138669	23.051489648524512	27.125892151136465	22.630173686472123
34	23.0536776714088	23.40400444654873	30.70233711233249	22.83998076970998
35	24.129881038411348	25.929043633430805	27.401583034556783	22.539492293601064
36	26.05181602860475	24.67798077092555	27.612848799717504	21.657354400752194
37	23.337938311125914	26.090957326868132	29.31640695037625	21.254697411629706
38	25.537630650797016	24.993724628811034	26.484193661905486	22.98445105848646
39	24.057372391162577	22.979163336516095	27.086446960805826	25.877017311515505
40	24.52099012897787	22.80169036923494	28.907915233562324	23.769404268224864
41	22.65041289815179	24.387338397795933	27.637160165098486	25.3250885389538
42	23.44126161399508	24.053361015874714	29.54779037038973	22.957586999740474
43	23.821552146967065	22.076543118333745	30.09236495492369	24.009539779775498
44	23.955446991802816	22.205089462785683	29.087576223727773	24.751887321683732
45	23.497967873746216	21.257614775475425	27.812080439014636	27.43233691176372
46	22.66408804117859	21.901805179657952	29.901034509375375	25.533072269788082
47	20.870152389716047	23.813590174804794	32.13622144250271	23.180035992976446
48	21.83014743019751	24.65093437693921	30.28083881503974	23.238079377823535
49	24.604864339542253	22.454402514767636	30.010921880897406	22.929811264792708
50	22.336613949496787	22.800292465725533	28.596000415724347	26.267093169053336
51	21.753627407660876	23.993372721797147	26.926052727704807	27.32694714283717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	1143.0
2	1996.0
3	4879.5
4	7763.0
5	5319.0
6	2875.0
7	3016.5
8	3158.0
9	3346.5
10	3535.0
11	3594.5
12	3654.0
13	3651.0
14	3648.0
15	3548.0
16	3448.0
17	3652.5
18	3857.0
19	3867.0
20	3877.0
21	4354.5
22	4832.0
23	5567.5
24	6303.0
25	7298.5
26	10481.5
27	12669.0
28	15574.5
29	18480.0
30	20447.0
31	22414.0
32	24959.5
33	27505.0
34	32659.0
35	37813.0
36	40788.5
37	43764.0
38	47850.0
39	51936.0
40	56859.0
41	61782.0
42	70125.5
43	78469.0
44	89131.0
45	99793.0
46	111168.5
47	122544.0
48	140631.5
49	158719.0
50	159652.0
51	160585.0
52	145347.5
53	130110.0
54	120337.5
55	110565.0
56	105447.0
57	100329.0
58	93419.5
59	86510.0
60	78466.5
61	70423.0
62	63683.0
63	56943.0
64	50655.0
65	44367.0
66	37958.5
67	31550.0
68	27365.0
69	23180.0
70	18990.0
71	14800.0
72	12120.0
73	9440.0
74	7644.0
75	4725.5
76	3603.0
77	2678.0
78	1753.0
79	1332.0
80	911.0
81	777.0
82	643.0
83	455.0
84	267.0
85	158.0
86	49.0
87	31.0
88	13.0
89	11.0
90	9.0
91	5.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1645321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.476848274799597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69402583982651	18.982318443706482
2	11.493219847892261	6.086084762031174
3	4.497207280127491	3.5721562444877932
4	2.2157367583044323	2.34662903866091
5	1.3605770741055672	1.8011896378631935
6	0.8665737026324283	1.3766484266118064
7	0.6392980184793128	1.1848617646359758
8	0.45692699543397086	0.9678389384612235
9	0.3872489532599019	0.9227818602033646
>10	4.996577145464212	34.85086889405602
>50	1.3049635253834897	22.054410355504057
>100	0.0820653458324659	3.802824721658914
>500	0.003719675505313696	0.7213431518926094
>1k	0.001859837752656848	1.3300437602265982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4582	0.2784866904391301	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	3800	0.23095797111931352	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	3778	0.22962084602335958	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	2938	0.1785669787233008	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	2429	0.1476307662760033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2155682690490184E-4	0.0	0.0	0.2514402964527895	0.0
2	1.2155682690490184E-4	0.0	0.0	0.8781265175610109	0.0
3	1.2155682690490184E-4	0.0	0.0	1.2990170307192335	0.0
4	1.2155682690490184E-4	0.0	0.0	1.82663443789996	0.0
5	1.2155682690490184E-4	0.0	0.0	2.686709766665593	0.0
6	1.2155682690490184E-4	0.0	0.0	3.306710362294045	0.0
7	1.2155682690490184E-4	0.0	0.0	3.8773588861991066	0.0
8	1.2155682690490184E-4	0.0	0.0	4.744727624579033	0.0
9	1.2155682690490184E-4	0.0	0.0	5.2039085382123	0.0
10	1.2155682690490184E-4	0.0	0.0	6.014874908908353	0.0
11	1.2155682690490184E-4	0.0	0.0	7.66610284558454	0.0
12	1.8233524035735275E-4	0.0	0.0	9.053430911050183	0.0
13	2.4311365380980368E-4	0.0	0.0	9.517838768240361	0.0
14	2.4311365380980368E-4	0.0	0.0	9.699505446049738	0.0
15	3.038920672622546E-4	0.0	0.0	9.955868793992176	0.0
16	3.646704807147055E-4	0.0	0.0	10.503664634439115	0.0
17	4.254488941671564E-4	0.0	0.0	11.229845118369	0.0
18	4.254488941671564E-4	0.0	0.0	12.038258795700049	0.0
19	4.254488941671564E-4	0.0	0.0	12.622825576285722	0.0
20	4.254488941671564E-4	0.0	0.0	13.286525851186486	0.0
21	4.254488941671564E-4	0.0	0.0	14.147999083461524	0.0
22	4.254488941671564E-4	0.0	0.0	15.071162405390803	0.0
23	4.254488941671564E-4	0.0	0.0	15.849612324889794	0.0
24	4.254488941671564E-4	0.0	0.0	16.482801836237428	0.0
25	4.254488941671564E-4	0.0	0.0	17.05150545091201	0.0
26	4.254488941671564E-4	0.0	0.0	17.568182743671297	0.0
27	4.254488941671564E-4	0.0	0.0	18.16314263295734	0.0
28	4.254488941671564E-4	0.0	0.0	18.71519296234595	0.0
29	4.8622730761960735E-4	0.0	0.0	19.351968400087276	0.0
30	4.8622730761960735E-4	0.0	0.0	20.002418980855406	0.0
31	4.8622730761960735E-4	0.0	0.0	20.55179506005211	0.0
32	4.8622730761960735E-4	0.0	0.0	21.11417771972764	0.0
33	4.8622730761960735E-4	0.0	0.0	21.665012480847203	0.0
34	4.8622730761960735E-4	0.0	0.0	22.27601787128469	0.0
35	4.8622730761960735E-4	0.0	0.0	22.905986126719345	0.0
36	4.8622730761960735E-4	0.0	0.0	23.457732564040693	0.0
37	4.8622730761960735E-4	0.0	0.0	24.07384334120819	0.0
38	4.8622730761960735E-4	0.0	0.0	24.802029512782003	0.0
39	4.8622730761960735E-4	0.0	0.0	26.190937816997412	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGA	30	2.1660744E-6	45.000004	32
CTCGTAG	25	3.8915423E-5	45.0	1
CGCTATA	25	3.8915423E-5	45.0	20
TAATCGC	20	7.0341746E-4	45.0	43
CGTTTTT	2535	0.0	40.029587	1
TCATACG	155	0.0	39.193546	31
CGCGCAT	145	0.0	38.793102	36
TTTGGGC	3875	0.0	38.67097	4
GTTACGT	70	0.0	38.57143	22
GCGCTAA	35	6.249933E-6	38.57143	9
TGGGCGA	2065	0.0	37.91768	6
TTCGGGC	700	0.0	37.607143	4
CGGGATA	355	0.0	37.394367	6
TTTGGGA	6470	0.0	37.31453	4
CCCGATC	140	0.0	36.964287	41
TTGGGAT	3220	0.0	36.754658	5
TCTTGCG	565	0.0	36.63717	1
TTGGGAC	2310	0.0	36.62338	5
TTGGGCA	1980	0.0	36.590908	5
CGTTTGG	990	0.0	36.363636	2
>>END_MODULE