SRR2934376.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1279383
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	5308	0.4148874887348042	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	3293	0.2573896948763584	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3183	0.24879180042254742	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	2845	0.22237281564629202	Illumina Paired End PCR Primer 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1898	0.1483527606666651	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	1469	0.11482097229680245	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	30	2.1656942E-6	45.000004	1
ACGTTAT	20	7.0335873E-4	45.0	15
CCGATAA	45	3.8562575E-10	45.0	15
CACGATC	20	7.0335873E-4	45.0	10
CGATGTA	25	3.891054E-5	45.0	21
CCCTCGT	165	0.0	43.636364	14
TACGCGC	65	0.0	41.538464	34
TAGCGAA	60	3.6379788E-12	41.250004	32
CTTTGCG	525	0.0	39.85714	1
TCGTTTG	490	0.0	39.489796	1
CGTTTTT	1700	0.0	39.308826	1
TGGGCGA	1710	0.0	39.07895	6
TTGGGAC	2200	0.0	38.965908	5
CGTTTGG	875	0.0	38.82857	2
TTACACG	70	0.0	38.57143	34
AATCGGT	35	6.2488416E-6	38.57143	26
TTTGGGC	3230	0.0	38.382355	4
GCGATTC	370	0.0	38.310814	9
TTTGGGA	5820	0.0	38.234535	4
TTCGGGA	1070	0.0	37.850468	4