##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1279383
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20864354145709	33.0	31.0	34.0	30.0	34.0
2	32.40809906025014	34.0	31.0	34.0	30.0	34.0
3	32.47276929582463	34.0	31.0	34.0	30.0	34.0
4	35.955014252964126	37.0	35.0	37.0	35.0	37.0
5	35.909845605264415	37.0	35.0	37.0	35.0	37.0
6	35.53222920736011	37.0	35.0	37.0	33.0	37.0
7	35.87104408922113	37.0	35.0	37.0	35.0	37.0
8	35.95944763999522	37.0	35.0	37.0	35.0	37.0
9	37.734344602046455	39.0	38.0	39.0	35.0	39.0
10	37.26607044176763	39.0	37.0	39.0	34.0	39.0
11	37.16500062920955	39.0	37.0	39.0	34.0	39.0
12	36.950291664028676	39.0	37.0	39.0	33.0	39.0
13	36.87288403863425	39.0	37.0	39.0	33.0	39.0
14	38.03456431733109	40.0	37.0	41.0	33.0	41.0
15	38.0832870219473	40.0	37.0	41.0	33.0	41.0
16	38.13365114277742	40.0	37.0	41.0	33.0	41.0
17	38.04915416259244	40.0	37.0	41.0	33.0	41.0
18	38.02479789085833	40.0	37.0	41.0	33.0	41.0
19	38.04594402145409	40.0	37.0	41.0	34.0	41.0
20	37.983728093932775	40.0	37.0	41.0	33.0	41.0
21	37.82516337953529	40.0	36.0	41.0	33.0	41.0
22	37.89014235768335	40.0	36.0	41.0	33.0	41.0
23	37.8482455996367	40.0	36.0	41.0	33.0	41.0
24	37.80614171049639	40.0	36.0	41.0	33.0	41.0
25	37.71852994763882	40.0	36.0	41.0	33.0	41.0
26	37.62734693207585	40.0	36.0	41.0	33.0	41.0
27	37.522969275033354	39.0	36.0	41.0	33.0	41.0
28	37.40400099110274	39.0	35.0	41.0	32.0	41.0
29	37.329412693462395	39.0	35.0	41.0	32.0	41.0
30	37.29741445681238	39.0	35.0	41.0	32.0	41.0
31	37.24919199332803	39.0	35.0	41.0	32.0	41.0
32	37.10461527158013	39.0	35.0	41.0	31.0	41.0
33	37.02037466497523	39.0	35.0	41.0	31.0	41.0
34	36.90886622692344	39.0	35.0	41.0	31.0	41.0
35	36.80395550042481	39.0	35.0	41.0	31.0	41.0
36	36.59743798377812	39.0	35.0	41.0	30.0	41.0
37	36.568352870094415	39.0	35.0	41.0	30.0	41.0
38	36.4893702667614	39.0	35.0	40.0	30.0	41.0
39	36.44157535311943	39.0	35.0	40.0	30.0	41.0
40	36.291796905226974	39.0	35.0	40.0	30.0	41.0
41	36.12171023063461	38.0	35.0	40.0	30.0	41.0
42	36.02426872953603	38.0	35.0	40.0	30.0	41.0
43	36.007804543283754	38.0	35.0	40.0	30.0	41.0
44	35.90942430843618	38.0	35.0	40.0	29.0	41.0
45	35.935054631802984	38.0	35.0	40.0	29.0	41.0
46	35.78779067722488	38.0	35.0	40.0	29.0	41.0
47	35.5383563795986	38.0	34.0	40.0	28.0	41.0
48	35.41518763341392	38.0	34.0	40.0	28.0	41.0
49	35.39426426644719	37.0	34.0	40.0	28.0	41.0
50	35.27402193088387	37.0	34.0	40.0	28.0	41.0
51	34.20589612336572	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	19.0
10	43.0
11	35.0
12	18.0
13	32.0
14	34.0
15	49.0
16	94.0
17	147.0
18	261.0
19	508.0
20	821.0
21	1437.0
22	2209.0
23	3387.0
24	4895.0
25	6972.0
26	9873.0
27	12355.0
28	15002.0
29	18328.0
30	22485.0
31	28796.0
32	37468.0
33	51770.0
34	87126.0
35	105702.0
36	113678.0
37	167448.0
38	268467.0
39	319802.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.648716607927415	4.6088622406269275	55.97377798516941	17.76864316627624
2	19.843315097980824	4.60065515955738	53.32812769905494	22.22790204340686
3	20.241553936545976	4.577362681855238	51.84866455158463	23.332418830014156
4	19.997608222088303	4.9345661150726565	49.74038266883334	25.327442994005704
5	18.218234883533704	5.887681796616025	50.30815635349227	25.585926966358002
6	22.09150817229868	6.635933102128135	52.744799641702286	18.527759083870897
7	83.64445986854601	2.5433353421141285	9.085004255957754	4.727200533382106
8	85.03442675101984	3.105559476716511	7.1457882432391235	4.714225529024538
9	79.66050822935743	5.282702677775146	9.273845283234184	5.782943809633237
10	40.51038664731359	27.08672852460913	16.747838606578327	15.655046221498958
11	28.930038932829337	25.33916739553363	25.728261200907	20.00253247073003
12	27.290107809780185	20.80526316200856	31.00361658705798	20.901012441153274
13	24.434121760254747	21.584857700938656	32.49456964802565	21.486450890780947
14	21.01622422683434	24.548786407197845	31.850978166819477	22.58401119914834
15	18.951713443120628	24.35564643269451	34.80005596447663	21.892584159708235
16	21.975202109141673	22.679682315616198	32.40241585201617	22.942699723225964
17	21.266813768824502	23.713383716994834	30.020564600279982	24.999237913900686
18	22.12167896556387	23.67375523983045	31.328304346704623	22.876261447901058
19	24.6056888359467	24.990952670154286	29.20517155535129	21.19818693854772
20	27.483482272314074	24.884573266957588	27.850299714784395	19.781644745943943
21	25.922808103593688	24.816180924711364	28.70110045232741	20.559910519367538
22	23.588714247414575	24.102477522368204	28.53023684072713	23.778571389490093
23	23.089645555709275	25.7324038227802	27.269941839152157	23.908008782358372
24	23.89776947169065	24.162037482130057	28.640837028473882	23.299356017705406
25	24.142574975593703	24.33884145717115	26.661132749145487	24.857450818089657
26	22.357417598951994	26.568588139751743	26.953539323251913	24.120454938044354
27	21.535615214521375	25.925621959960388	29.0492370150299	23.489525810488338
28	20.34840231580379	24.801798992170447	30.49532469948405	24.354473992541717
29	22.667254449996598	23.53736136872227	29.847043457666704	23.948340723614432
30	24.34493814596567	23.50844117828672	29.540411276373064	22.606209399374542
31	25.048871213702228	25.60335724329618	26.198487864853604	23.149283678147984
32	24.86276588011565	25.327442994005704	26.792915022319352	23.016876103559294
33	24.27599866498148	25.35104812241526	26.68770805927545	23.685245153327816
34	23.18899031798922	24.515332781504835	28.295514322138093	24.000162578367853
35	23.56768848734116	23.664297555931256	28.252446687192183	24.515567269535392
36	23.850012076133574	25.97634953723787	28.1036249504644	22.070013436164153
37	22.700239099628494	26.35848686437134	28.750186613390987	22.19108742260918
38	21.87531020812376	26.242962427982864	28.232202553887305	23.649524810006074
39	23.905976552760198	24.616944261413508	28.05289737318692	23.424181812639375
40	25.263349598986384	23.151003257038745	29.380334114178474	22.205313029796393
41	21.57039760572088	24.025956261729288	29.64256989501971	24.761076237530123
42	21.780342555747577	25.348077940694854	27.851159504229773	25.020419999327803
43	22.243534578777428	24.307967199814286	28.720875609571177	24.727622611837113
44	22.76784981510619	23.47827038502153	28.0620424063787	25.691837393493582
45	23.895268266031362	22.328028432455334	27.569070403467922	26.207632898045386
46	23.91879523176406	23.809758297554367	28.42088725581003	23.850559214871545
47	21.53272319547782	24.448894506179933	30.23926377011419	23.77911852822806
48	20.9708898742597	22.696096477755294	31.27296517149282	25.060048476492184
49	22.395717310609882	22.692422831943208	31.123596296027067	23.78826356141984
50	22.101122181551577	22.16138560540511	29.67109927207099	26.066392940972328
51	21.43486352405808	22.998351549145173	27.72078415923926	27.846000767557488
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	1019.5
2	1779.0
3	3748.0
4	5717.0
5	3940.5
6	2164.0
7	2154.5
8	2145.0
9	2376.5
10	2608.0
11	2668.5
12	2729.0
13	2726.0
14	2723.0
15	2672.0
16	2621.0
17	2591.0
18	2561.0
19	2697.0
20	2833.0
21	3319.5
22	3806.0
23	4868.5
24	5931.0
25	6762.0
26	9142.0
27	10691.0
28	13453.0
29	16215.0
30	18275.5
31	20336.0
32	23116.0
33	25896.0
34	28996.5
35	32097.0
36	34120.5
37	36144.0
38	40510.5
39	44877.0
40	50196.5
41	55516.0
42	61004.5
43	66493.0
44	74613.5
45	82734.0
46	92219.0
47	101704.0
48	115305.5
49	128907.0
50	125514.0
51	122121.0
52	107970.5
53	93820.0
54	84333.0
55	74846.0
56	70304.5
57	65763.0
58	64002.0
59	62241.0
60	59020.5
61	55800.0
62	49594.0
63	43388.0
64	37212.5
65	31037.0
66	25881.0
67	20725.0
68	18157.0
69	15589.0
70	13099.0
71	10609.0
72	8571.0
73	6533.0
74	5432.5
75	3439.5
76	2547.0
77	1901.5
78	1256.0
79	1117.0
80	978.0
81	706.5
82	435.0
83	310.5
84	186.0
85	129.5
86	73.0
87	46.0
88	19.0
89	11.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1279383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.025121720793198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5501533448978	19.336516032872815
2	11.716993403871841	6.333063458827351
3	4.756611549632026	3.8564401832200876
4	2.39939152778576	2.593753923770005
5	1.3683604521630635	1.8490053888813207
6	0.9036549119094781	1.465283039276778
7	0.6253503123173983	1.1830117815959615
8	0.4624721165901803	0.9998692194657988
9	0.3569273326195347	0.8681404148568871
>10	4.425722640476054	30.921831637512927
>50	1.3226134827009322	23.481509660059935
>100	0.10503815408147525	4.697681620604153
>500	0.004668362403047118	0.909241418742167
>1k	0.0017506359011426691	1.0861062615345252
>5k	2.9177265019044487E-4	0.41854595877924056
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	5308	0.4148874887348042	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	3293	0.2573896948763584	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3183	0.24879180042254742	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	2845	0.22237281564629202	Illumina Paired End PCR Primer 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1898	0.1483527606666651	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	1469	0.11482097229680245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3448803055848015E-4	0.0	0.0	0.24597794405584567	0.0
2	2.3448803055848015E-4	0.0	0.0	1.1965924199399243	0.0
3	2.3448803055848015E-4	0.0	0.0	1.7846102379037396	0.0
4	2.3448803055848015E-4	0.0	0.0	2.42585683880433	0.0
5	2.3448803055848015E-4	0.0	0.0	3.4143802129620293	0.0
6	2.3448803055848015E-4	0.0	0.0	4.121596113126405	0.0
7	3.1265070741130687E-4	0.0	0.0	4.761670273874204	0.0
8	3.1265070741130687E-4	0.0	0.0	5.789587637165727	0.0
9	3.908133842641336E-4	0.0	0.0	6.311479830512051	0.0
10	3.908133842641336E-4	0.0	0.0	7.231767187777233	0.0
11	3.908133842641336E-4	0.0	0.0	8.974325905534153	0.0
12	3.908133842641336E-4	0.0	0.0	10.445816459965467	0.0
13	3.908133842641336E-4	0.0	0.0	10.9375378600466	0.0
14	3.908133842641336E-4	0.0	0.0	11.108010658262616	0.0
15	3.908133842641336E-4	0.0	0.0	11.362742822125979	0.0
16	4.689760611169603E-4	0.0	0.0	11.954356123225024	0.0
17	4.689760611169603E-4	0.0	0.0	12.786241492969658	0.0
18	5.47138737969787E-4	0.0	0.0	13.731228256120334	0.0
19	5.47138737969787E-4	0.0	0.0	14.353793977253098	7.816267685282672E-5
20	6.253014148226137E-4	0.0	0.0	15.031698873597664	7.816267685282672E-5
21	6.253014148226137E-4	0.0	0.0	15.991302057319817	7.816267685282672E-5
22	6.253014148226137E-4	0.0	0.0	16.931599059859323	7.816267685282672E-5
23	6.253014148226137E-4	0.0	0.0	17.821012159767637	7.816267685282672E-5
24	6.253014148226137E-4	0.0	0.0	18.530572940237597	7.816267685282672E-5
25	7.034640916754405E-4	0.0	0.0	19.14477525494711	7.816267685282672E-5
26	7.034640916754405E-4	0.0	0.0	19.700355562017002	7.816267685282672E-5
27	7.034640916754405E-4	0.0	0.0	20.231470951231962	7.816267685282672E-5
28	7.816267685282672E-4	0.0	0.0	20.82644524743568	7.816267685282672E-5
29	7.816267685282672E-4	0.0	0.0	21.444164882603566	7.816267685282672E-5
30	7.816267685282672E-4	0.0	0.0	22.096745071647817	7.816267685282672E-5
31	7.816267685282672E-4	0.0	0.0	22.685935329764426	7.816267685282672E-5
32	7.816267685282672E-4	0.0	0.0	23.274031310405093	7.816267685282672E-5
33	7.816267685282672E-4	0.0	0.0	23.87119416156069	7.816267685282672E-5
34	7.816267685282672E-4	0.0	0.0	24.48477117485538	7.816267685282672E-5
35	8.597894453810939E-4	0.0	0.0	25.122891268681858	7.816267685282672E-5
36	8.597894453810939E-4	0.0	0.0	25.742017832033095	7.816267685282672E-5
37	8.597894453810939E-4	0.0	0.0	26.355516682650933	7.816267685282672E-5
38	8.597894453810939E-4	0.0	0.0	26.98449174328563	7.816267685282672E-5
39	8.597894453810939E-4	0.0	0.0	27.625972832216778	7.816267685282672E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	30	2.1656942E-6	45.000004	1
ACGTTAT	20	7.0335873E-4	45.0	15
CCGATAA	45	3.8562575E-10	45.0	15
CACGATC	20	7.0335873E-4	45.0	10
CGATGTA	25	3.891054E-5	45.0	21
CCCTCGT	165	0.0	43.636364	14
TACGCGC	65	0.0	41.538464	34
TAGCGAA	60	3.6379788E-12	41.250004	32
CTTTGCG	525	0.0	39.85714	1
TCGTTTG	490	0.0	39.489796	1
CGTTTTT	1700	0.0	39.308826	1
TGGGCGA	1710	0.0	39.07895	6
TTGGGAC	2200	0.0	38.965908	5
CGTTTGG	875	0.0	38.82857	2
TTACACG	70	0.0	38.57143	34
AATCGGT	35	6.2488416E-6	38.57143	26
TTTGGGC	3230	0.0	38.382355	4
GCGATTC	370	0.0	38.310814	9
TTTGGGA	5820	0.0	38.234535	4
TTCGGGA	1070	0.0	37.850468	4
>>END_MODULE