##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436085
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.218668811386515	33.0	31.0	34.0	30.0	34.0
2	32.40623779233123	34.0	31.0	34.0	30.0	34.0
3	32.48213162869886	34.0	31.0	34.0	30.0	34.0
4	35.96174042622825	37.0	35.0	37.0	35.0	37.0
5	35.91865801815352	37.0	35.0	37.0	35.0	37.0
6	35.57120713606785	37.0	35.0	37.0	33.0	37.0
7	35.94458754182378	37.0	35.0	37.0	35.0	37.0
8	36.04432606705035	37.0	35.0	37.0	35.0	37.0
9	37.834692932521406	39.0	38.0	39.0	35.0	39.0
10	37.29032195169506	39.0	37.0	39.0	34.0	39.0
11	37.191071559134734	39.0	37.0	39.0	34.0	39.0
12	36.93753085646045	39.0	37.0	39.0	33.0	39.0
13	36.807787839856275	39.0	35.0	39.0	33.0	39.0
14	37.64534968334047	40.0	37.0	41.0	32.0	41.0
15	37.77694077996776	40.0	37.0	41.0	33.0	41.0
16	37.92482199869785	40.0	37.0	41.0	33.0	41.0
17	37.91445004996223	40.0	37.0	41.0	33.0	41.0
18	37.917667826068794	40.0	36.0	41.0	33.0	41.0
19	37.968973981345115	40.0	37.0	41.0	33.0	41.0
20	37.894940759077635	40.0	36.0	41.0	33.0	41.0
21	37.74754140597528	40.0	36.0	41.0	33.0	41.0
22	37.91600009748726	40.0	36.0	41.0	33.0	41.0
23	37.864821371994	40.0	36.0	41.0	33.0	41.0
24	37.837101564322445	40.0	36.0	41.0	33.0	41.0
25	37.76237618246831	40.0	36.0	41.0	33.0	41.0
26	37.70454743277731	40.0	36.0	41.0	33.0	41.0
27	37.64548546917487	40.0	36.0	41.0	33.0	41.0
28	37.54753304992393	39.0	36.0	41.0	33.0	41.0
29	37.45995118673338	39.0	35.0	41.0	33.0	41.0
30	37.42374093455471	39.0	35.0	41.0	32.0	41.0
31	37.33584920112668	39.0	35.0	41.0	32.0	41.0
32	37.23333089615169	39.0	35.0	41.0	32.0	41.0
33	37.20509510230941	39.0	35.0	41.0	32.0	41.0
34	37.028088170268475	39.0	35.0	41.0	31.0	41.0
35	36.9633642855402	39.0	35.0	41.0	31.0	41.0
36	36.82715647054318	39.0	35.0	41.0	31.0	41.0
37	36.794716190197654	39.0	35.0	41.0	31.0	41.0
38	36.745933562428405	39.0	35.0	41.0	31.0	41.0
39	36.69160321290175	39.0	35.0	41.0	31.0	41.0
40	36.5719160077572	39.0	35.0	41.0	30.0	41.0
41	36.46642643019042	39.0	35.0	41.0	30.0	41.0
42	36.42023626735186	39.0	35.0	41.0	30.0	41.0
43	36.377424734608326	39.0	35.0	41.0	30.0	41.0
44	36.24272588321722	39.0	35.0	40.0	30.0	41.0
45	36.27507285432269	38.0	35.0	40.0	30.0	41.0
46	36.23136165338403	38.0	35.0	40.0	30.0	41.0
47	36.04907091154075	38.0	35.0	40.0	30.0	41.0
48	35.95892861494967	38.0	35.0	40.0	29.0	41.0
49	35.97325297597287	38.0	35.0	40.0	29.0	41.0
50	35.88658749308014	38.0	35.0	40.0	29.0	41.0
51	34.97744075037341	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	21.0
11	19.0
12	14.0
13	19.0
14	31.0
15	38.0
16	96.0
17	148.0
18	319.0
19	547.0
20	969.0
21	1606.0
22	2360.0
23	3597.0
24	5270.0
25	7551.0
26	10219.0
27	13126.0
28	15993.0
29	19387.0
30	24300.0
31	31354.0
32	42248.0
33	60735.0
34	99466.0
35	101850.0
36	113095.0
37	173727.0
38	302373.0
39	405415.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.02651305458939	4.43114439604898	55.24679945824934	15.295543091112297
2	19.490420135298397	4.098155749833749	54.165735315110176	22.245688799757673
3	21.845503573952797	4.378710173840685	52.193289394429996	21.582496857776523
4	19.472384991139105	4.750415191301351	51.03430507247134	24.742894745088208
5	18.329834236831385	5.9857877493323866	51.23213458813372	24.45224342570252
6	20.929610712457826	6.381934216985763	54.52114603244237	18.167309038114038
7	85.82590863354189	1.8439019974444408	8.149865780925223	4.1803235880884495
8	87.95948707771475	2.2564820327487576	6.139539094134401	3.644491795402083
9	82.88548379796461	4.996361635975586	8.050080601078626	4.068073964981181
10	37.94858939408183	35.6537391588938	14.200900364532739	12.196771082491635
11	28.30542760351929	24.218552523005254	29.05830782996828	18.417712043507173
12	25.497306914284323	23.190061869596857	31.983343604313113	19.329287611805707
13	24.60766598077412	22.946343705282068	33.227559650020716	19.218430663923098
14	20.060720639794997	25.85355323675131	34.70706817493394	19.37865794851976
15	18.358662613981764	25.286246983987716	36.54177851589564	19.813311886134873
16	24.334423101696625	25.948046250744213	30.55424992253244	19.16328072502672
17	24.743451815178073	24.7055014153062	29.631602586197893	20.91944418331784
18	25.43428836036864	23.414561115811388	31.671314720228956	19.479835803591016
19	24.53099920965681	25.551481980523437	29.588638555517257	20.328880254302494
20	24.576748590786757	29.107469265398635	28.77315757771998	17.542624566094624
21	23.33879958359011	26.95606457835017	32.308045832941644	17.39709000511808
22	22.157741359320653	22.669062068053076	34.05404276209277	21.1191538105335
23	20.601217894483963	25.77723463444016	32.63114648506182	20.99040098601406
24	21.564531347378463	27.199434573858788	30.392978131517285	20.84305594724546
25	23.091251562407518	27.37895041031694	27.68429445332275	21.845503573952797
26	20.297266526702806	27.83400703997326	27.988593989910065	23.880132443413864
27	19.566529836325845	27.05222880261266	31.70153577260399	21.679705588457505
28	17.42696288868695	27.54112744022812	31.16194375681105	23.869965914273877
29	20.14219214043737	28.338016203776238	29.790506829331132	21.72928482645526
30	23.259974165874585	26.03620259246493	31.100526779403726	19.60329646225676
31	22.674980937757862	27.262174592729536	29.50946496899557	20.55337950051703
32	23.52221491067729	27.665284436506195	28.303477858204772	20.509022794611738
33	24.130187280000836	26.34690843508567	27.643837238046494	21.879067046867004
34	19.905506986007097	26.19078954240174	31.621387313425043	22.282316158166125
35	19.780932187161625	29.642186917905278	28.67915200005571	21.897728894877392
36	22.159203668306542	30.237068140117056	27.46766382212752	20.136064369448885
37	20.53645849653746	29.953589098138337	28.36398959671607	21.145962808608125
38	22.568302015549218	29.072652384782238	27.924461295814663	20.43458430385388
39	21.645515411692205	25.924788574492457	27.86986842700815	24.559827586807188
40	22.728250765100952	26.3386916512602	28.2005591591027	22.732498424536153
41	19.67341765981819	26.942207459864843	27.235713763461078	26.148661116855898
42	21.151185340700586	26.986912334576296	29.291859465143077	22.570042859580038
43	21.611882305016763	24.749301051121623	30.36972045526553	23.269096188596077
44	22.141447059192178	24.726113008631103	29.146533805450236	23.985906126726483
45	21.360782962011303	25.304839198236873	27.509583346389665	25.824794493362162
46	20.70928949191726	26.331589007614454	29.287124369379246	23.671997131089036
47	18.671178934394554	27.963177667060098	31.18185901252363	22.183784386021717
48	19.224558434911582	29.175153281316913	29.64274398799514	21.957544295776366
49	22.69238937806606	25.148581038030475	30.165136464763577	21.993893119139884
50	20.497185055202163	25.335478053179305	28.20661729632995	25.960719595288577
51	18.95974124094326	25.978754739447872	27.30896848027798	27.752535539330893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	934.5
2	1611.0
3	3911.5
4	6212.0
5	4296.5
6	2381.0
7	2345.0
8	2309.0
9	2371.5
10	2434.0
11	2698.0
12	2962.0
13	2689.5
14	2417.0
15	2559.0
16	2701.0
17	2975.0
18	3249.0
19	3483.0
20	3717.0
21	4327.5
22	4938.0
23	6658.5
24	8379.0
25	10478.0
26	14635.0
27	16693.0
28	19162.0
29	21631.0
30	26563.5
31	31496.0
32	35761.5
33	40027.0
34	44788.0
35	49549.0
36	55555.0
37	61561.0
38	65185.5
39	68810.0
40	76927.0
41	85044.0
42	90659.5
43	96275.0
44	106049.0
45	115823.0
46	130012.0
47	144201.0
48	143562.0
49	142923.0
50	127601.5
51	112280.0
52	100525.0
53	88770.0
54	82413.5
55	76057.0
56	68997.0
57	61937.0
58	57122.5
59	52308.0
60	44210.0
61	36112.0
62	30784.0
63	25456.0
64	22674.5
65	19893.0
66	16258.0
67	12623.0
68	10260.5
69	7898.0
70	6498.5
71	5099.0
72	4032.0
73	2965.0
74	2690.5
75	1746.5
76	1077.0
77	747.5
78	418.0
79	388.5
80	359.0
81	231.0
82	103.0
83	95.0
84	87.0
85	58.0
86	29.0
87	23.5
88	18.0
89	9.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1436085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.88155893090265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.18526729246506	18.423856904419917
2	11.518499857606951	5.962334657204962
3	4.815373199621492	3.7388809572087873
4	2.4871143503452515	2.5748158650541715
5	1.4534790788690202	1.8809152217291323
6	0.9263726453273868	1.4385580927210164
7	0.685428469680595	1.241797013166972
8	0.5052074877544989	1.0460445893321073
9	0.3857293732109705	0.8984949753725888
>10	4.423575539371567	28.25644248899426
>50	1.4544053627723124	25.66421551754694
>100	0.1527638675220308	6.496397397878873
>500	0.004884102326071776	0.8599267726415205
>1k	0.0018993731268056903	1.5173195467286804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	4513	0.31425716444360885	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4214	0.2934366698350028	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3998	0.27839577740871885	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3586	0.24970666778080683	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2766	0.19260698356991404	No Hit
TCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1485	0.10340613543070223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.96337612327961E-5	0.0	0.0	0.34454785057987514	0.0
2	6.96337612327961E-5	0.0	0.0	1.2159447386470856	0.0
3	6.96337612327961E-5	0.0	0.0	1.6803322923086028	0.0
4	6.96337612327961E-5	0.0	0.0	2.2521647395523248	0.0
5	1.392675224655922E-4	0.0	0.0	3.1839340986083693	0.0
6	1.392675224655922E-4	0.0	0.0	3.816904988214486	0.0
7	1.392675224655922E-4	0.0	0.0	4.370005953686586	0.0
8	1.392675224655922E-4	0.0	0.0	5.245720134950229	0.0
9	1.392675224655922E-4	0.0	0.0	5.703144312488467	0.0
10	1.392675224655922E-4	0.0	0.0	6.56590661416281	0.0
11	1.392675224655922E-4	0.0	0.0	8.098824233941585	0.0
12	1.392675224655922E-4	0.0	0.0	9.45675221174234	0.0
13	1.392675224655922E-4	0.0	0.0	9.90623814050004	0.0
14	2.0890128369838832E-4	0.0	0.0	10.071200520860534	0.0
15	2.785350449311844E-4	0.0	0.0	10.301131200451227	0.0
16	3.4816880616398056E-4	0.0	0.0	10.85283949069867	0.0
17	3.4816880616398056E-4	0.0	0.0	11.653558111114593	0.0
18	3.4816880616398056E-4	0.0	0.0	12.586511244111595	0.0
19	3.4816880616398056E-4	0.0	0.0	13.217532388403193	0.0
20	3.4816880616398056E-4	0.0	0.0	13.91547157723951	0.0
21	3.4816880616398056E-4	0.0	0.0	14.806992622302998	0.0
22	3.4816880616398056E-4	0.0	0.0	15.779706632963926	0.0
23	3.4816880616398056E-4	0.0	0.0	16.679444461852885	0.0
24	3.4816880616398056E-4	0.0	0.0	17.331634269559252	0.0
25	3.4816880616398056E-4	0.0	0.0	17.912728007046937	0.0
26	3.4816880616398056E-4	0.0	0.0	18.44375507020824	0.0
27	3.4816880616398056E-4	0.0	0.0	19.04845465275384	0.0
28	3.4816880616398056E-4	0.0	0.0	19.66178882169231	0.0
29	3.4816880616398056E-4	0.0	0.0	20.363000797306565	0.0
30	4.1780256739677664E-4	0.0	0.0	21.14053137523197	0.0
31	4.1780256739677664E-4	0.0	0.0	21.739938791923876	0.0
32	5.570700898623689E-4	0.0	0.0	22.3417137564977	0.0
33	6.26703851095165E-4	0.0	0.0	22.92273786022415	0.0
34	6.26703851095165E-4	0.0	0.0	23.575624005542846	0.0
35	6.26703851095165E-4	0.0	0.0	24.299258052274066	0.0
36	6.26703851095165E-4	0.0	0.0	24.89156282532023	0.0
37	6.26703851095165E-4	0.0	0.0	25.501833108764455	0.0
38	6.26703851095165E-4	0.0	0.0	26.26230341518782	0.0
39	6.26703851095165E-4	0.0	0.0	27.674963529317555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	80	0.0	45.0	22
CGTATTA	20	7.0338754E-4	45.0	36
CGATTAC	20	7.0338754E-4	45.0	2
TGCGAAG	25	3.891294E-5	45.0	1
GCTTACG	25	3.891294E-5	45.0	1
CTACGAA	125	0.0	43.2	11
TTTTGCG	975	0.0	42.461536	1
TACGGGA	320	0.0	42.1875	4
CGTTTTT	1960	0.0	40.982143	1
TCGACGG	55	6.184564E-11	40.909092	2
TGTTGCG	250	0.0	40.500004	1
CGCGCAT	50	1.0822987E-9	40.5	36
TCCGATA	90	0.0	40.0	14
TTATGCG	45	1.9288564E-8	40.0	1
ACGAACG	90	0.0	40.0	21
GTTTGCG	305	0.0	39.836067	1
TACGAAT	130	0.0	39.807693	12
TGGGCGA	1730	0.0	39.797688	6
GCGATAT	85	0.0	39.705883	9
CGTTTGG	1045	0.0	39.401913	2
>>END_MODULE