##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1146575
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08362209188235	33.0	31.0	34.0	30.0	34.0
2	32.26497786887033	34.0	31.0	34.0	30.0	34.0
3	32.3211216012908	34.0	31.0	34.0	30.0	34.0
4	35.870556221790984	37.0	35.0	37.0	35.0	37.0
5	35.84328456489981	37.0	35.0	37.0	35.0	37.0
6	35.486270850140635	37.0	35.0	37.0	33.0	37.0
7	35.85385256088786	37.0	35.0	37.0	35.0	37.0
8	35.9380162658352	37.0	35.0	37.0	35.0	37.0
9	37.739822514881276	39.0	38.0	39.0	35.0	39.0
10	37.12436866319255	39.0	37.0	39.0	33.0	39.0
11	37.02794409436801	39.0	37.0	39.0	33.0	39.0
12	36.960408172164925	39.0	37.0	39.0	33.0	39.0
13	36.94605237337287	39.0	37.0	39.0	33.0	39.0
14	38.120355842400194	40.0	37.0	41.0	33.0	41.0
15	38.12251270086998	40.0	37.0	41.0	33.0	41.0
16	38.159380764450646	40.0	37.0	41.0	33.0	41.0
17	38.08272725290539	40.0	37.0	41.0	33.0	41.0
18	38.072067679829054	40.0	37.0	41.0	33.0	41.0
19	38.08445151865338	40.0	37.0	41.0	34.0	41.0
20	38.032882279833416	40.0	37.0	41.0	34.0	41.0
21	37.896538822144215	40.0	37.0	41.0	33.0	41.0
22	37.98590846651985	40.0	37.0	41.0	33.0	41.0
23	37.925000109020345	40.0	37.0	41.0	33.0	41.0
24	37.89689728103264	40.0	37.0	41.0	33.0	41.0
25	37.827344046399055	40.0	37.0	41.0	33.0	41.0
26	37.78782286374638	40.0	37.0	41.0	33.0	41.0
27	37.71157752436605	40.0	36.0	41.0	33.0	41.0
28	37.61780084163705	39.0	36.0	41.0	33.0	41.0
29	37.56137627281251	39.0	36.0	41.0	33.0	41.0
30	37.513935852430066	39.0	36.0	41.0	33.0	41.0
31	37.45596145040665	39.0	36.0	41.0	32.0	41.0
32	37.32059655931797	39.0	36.0	41.0	32.0	41.0
33	37.25908204871029	39.0	36.0	41.0	32.0	41.0
34	37.09858491594532	39.0	36.0	41.0	31.0	41.0
35	37.04674443451148	39.0	36.0	41.0	31.0	41.0
36	36.8816422824499	39.0	35.0	41.0	31.0	41.0
37	36.82481215794867	39.0	35.0	41.0	31.0	41.0
38	36.75599502867235	39.0	35.0	41.0	30.0	41.0
39	36.70290909883784	39.0	35.0	41.0	30.0	41.0
40	36.54351001896954	39.0	35.0	40.0	30.0	41.0
41	36.498466301811916	39.0	35.0	40.0	30.0	41.0
42	36.47959357216057	39.0	35.0	40.0	30.0	41.0
43	36.41391710093103	39.0	35.0	40.0	30.0	41.0
44	36.28250310707978	39.0	35.0	40.0	30.0	41.0
45	36.36773957220417	39.0	35.0	40.0	30.0	41.0
46	36.268352266532936	39.0	35.0	40.0	30.0	41.0
47	36.044639033643676	38.0	35.0	40.0	29.0	41.0
48	35.965730109238386	38.0	35.0	40.0	29.0	41.0
49	35.95841179164032	38.0	35.0	40.0	29.0	41.0
50	35.82526219392539	38.0	35.0	40.0	29.0	41.0
51	34.93298911976975	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	11.0
10	23.0
11	10.0
12	21.0
13	19.0
14	22.0
15	27.0
16	50.0
17	81.0
18	153.0
19	298.0
20	608.0
21	1043.0
22	1616.0
23	2691.0
24	3973.0
25	6045.0
26	8360.0
27	10127.0
28	11960.0
29	14480.0
30	18451.0
31	24158.0
32	31695.0
33	43606.0
34	67649.0
35	85890.0
36	102845.0
37	156240.0
38	263127.0
39	291208.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.426487582582908	4.587401609140265	58.39068530187733	12.595425506399494
2	20.53271700499313	5.517824826112553	53.06613174018272	20.883326428711598
3	19.369949632601443	5.159017072585745	53.49479972962955	21.97623356518326
4	18.011905021477006	4.240542485227743	53.641497503434145	24.106054989861107
5	17.268299064605454	5.369906024464164	53.50352135708524	23.858273553845148
6	19.5864204260515	6.796502627390271	56.28947081525413	17.3276061313041
7	82.21930532237316	1.9938512526437433	10.945119159235112	4.841724265747989
8	84.37380895275058	2.786123890718008	7.95621743017247	4.883849726358939
9	79.52109543640843	4.406689488258509	10.281490526132178	5.790724549200881
10	37.027407714279484	24.47000850358677	21.287224996184285	17.21535878594946
11	24.67679829056102	27.268255456468175	26.138891917231753	21.916054335739048
12	21.113315744717966	23.091642500490593	33.36449861544164	22.430543139349805
13	22.45321937073458	24.146348908706365	34.79911911562697	18.60131260493208
14	20.708370581950593	26.167934936659183	32.921963238340275	20.201731243049952
15	17.956697119682534	26.892178880579117	34.39940692933302	20.751717070405338
16	19.45838693500207	25.654928809715894	31.41268560713429	23.473998648147745
17	19.49205241698101	26.846564768985893	30.06388592111288	23.59749689292022
18	20.806576107101584	24.5418747138216	32.75014717746331	21.9014020016135
19	21.227569064387414	28.081634432985197	29.98050716263655	20.710289339990844
20	22.96256241414648	27.293286527265987	30.418332860911846	19.325818197675687
21	22.507991191156272	28.355057453720867	29.910908575540198	19.22604277958267
22	21.38403506094237	25.377406624076055	29.581754355362712	23.656803959618866
23	20.715958397837035	27.002071386520726	28.101694176133268	24.18027603950897
24	21.174105488084077	26.41632688659704	29.468460414713388	22.941107210605498
25	19.445304493818547	28.45073370690971	28.116869807906152	23.98709199136559
26	19.00686828162135	27.863157665220328	30.4636853236814	22.66628872947692
27	20.432156640429103	27.32738809061771	30.51741054880841	21.72304472014478
28	18.109412816431547	25.702810544447598	33.0027255085799	23.18505113054096
29	19.324422737282777	24.458234306521597	31.807688114602183	24.409654841593444
30	20.94612214639252	27.498244772474546	29.98050716263655	21.57512591849639
31	19.347098968667552	29.86555611277064	28.28824978741033	22.499095131151474
32	18.718705710485576	30.23038178924187	27.71279680788435	23.3381156923882
33	20.819832980834224	28.735756492161435	26.54837232627608	23.896038200728256
34	18.2588142947474	28.807361053572595	29.327431698754985	23.606392952925013
35	18.994919652007063	27.559383380938883	28.65726184506029	24.788435121993764
36	22.371061640102045	27.470509997165472	28.97769443778209	21.180733924950395
37	19.905893639753177	27.855831498157556	30.197326821184834	22.040948040904432
38	18.345856136755117	29.671151036783467	28.335172143121905	23.64782068333951
39	19.52920654994222	28.481084970455488	28.061225824738894	23.928482654863398
40	19.489959226391644	30.549506137845324	27.642587706866102	22.31794692889693
41	19.472864836578506	29.714933606610995	27.20711684800384	23.605084708806665
42	22.226195408063145	27.684974816300723	27.35076205219894	22.738067723437194
43	22.621808429452937	25.153522447288662	29.22408041340514	23.000588709853258
44	19.864204260515013	24.594378911104812	29.324117480321828	26.217299348058347
45	20.51431437106164	24.988945337199922	28.209144626387285	26.287595665351155
46	21.73839478446678	26.762837145411332	28.811896299849554	22.686871770272333
47	18.801386738765455	27.65889715020823	31.183568453873495	22.356147657152825
48	19.240171816060876	26.793886139153567	30.29099710005887	23.674944944726686
49	20.809279811612846	24.98711379543423	30.856332991736256	23.34727340121667
50	20.313455290757254	24.69406711292327	28.701742145084275	26.290735451235204
51	19.0700128644005	25.360835531910254	26.94712513354992	28.62202647013933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	573.0
2	972.0
3	3121.5
4	5271.0
5	3635.0
6	1999.0
7	2083.5
8	2168.0
9	2270.0
10	2372.0
11	2555.5
12	2739.0
13	2888.5
14	3038.0
15	3039.5
16	3041.0
17	3151.5
18	3262.0
19	3203.5
20	3145.0
21	3634.5
22	4124.0
23	4950.0
24	5776.0
25	6106.5
26	8220.5
27	10004.0
28	11818.5
29	13633.0
30	15981.0
31	18329.0
32	21421.5
33	24514.0
34	28591.5
35	32669.0
36	37512.0
37	42355.0
38	47682.5
39	53010.0
40	61316.0
41	69622.0
42	79689.5
43	89757.0
44	109353.5
45	128950.0
46	140295.5
47	151641.0
48	142361.0
49	133081.0
50	114551.0
51	96021.0
52	81076.5
53	66132.0
54	57017.0
55	47902.0
56	42027.0
57	36152.0
58	31922.5
59	27693.0
60	24200.5
61	20708.0
62	17584.5
63	14461.0
64	11776.5
65	9092.0
66	7664.5
67	6237.0
68	4910.0
69	3583.0
70	2961.5
71	2340.0
72	2094.0
73	1848.0
74	1610.5
75	891.5
76	410.0
77	354.0
78	298.0
79	236.0
80	174.0
81	106.5
82	39.0
83	26.5
84	14.0
85	11.5
86	9.0
87	7.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1146575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.64419996563728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75426256314896	23.804705580957872
2	12.475085777922777	8.394285610018231
3	5.234799131877982	5.283618863185417
4	2.664163801875102	3.585346387659935
5	1.5511545670224582	2.609367721525755
6	1.031928425667495	2.083104378202948
7	0.7323827552896872	1.7248302309245442
8	0.5462978744022433	1.470380394175334
9	0.4451641825323817	1.3479473497193015
>10	3.9851800721425477	29.49657901788912
>50	0.4915620142568443	10.764812561148565
>100	0.07939977892126632	4.987413159793315
>500	0.005484853143774639	1.2466555747910522
>1k	0.0026118348303688756	2.1970030789402895
>5k	5.223669660737751E-4	1.003950091068427
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	5948	0.5187624010640385	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	5477	0.47768353574777056	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3983	0.3473824215598631	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3800	0.33142184331596275	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3224	0.2811852691712273	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	2634	0.2297276671826963	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	2597	0.2265006650240935	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	2491	0.21725573992106925	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	2046	0.17844449774327892	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1730	0.1508841549833199	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1387	0.1209689728103264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.616488236704969E-4	0.0	0.0	0.5643765126572619	0.0
2	2.616488236704969E-4	0.0	0.0	1.867038789438109	0.0
3	2.616488236704969E-4	0.0	0.0	2.6375073588731657	0.0
4	2.616488236704969E-4	0.0	0.0	3.6055207901794475	0.0
5	2.616488236704969E-4	0.0	0.0	5.171401783572815	0.0
6	2.616488236704969E-4	0.0	0.0	6.211368641388483	0.0
7	2.616488236704969E-4	0.0	0.0	7.104376076575889	0.0
8	2.616488236704969E-4	0.0	0.0	8.491899788500534	0.0
9	2.616488236704969E-4	0.0	0.0	9.108606065891895	0.0
10	2.616488236704969E-4	0.0	0.0	10.636460763578484	0.0
11	2.616488236704969E-4	0.0	0.0	13.228702875956653	0.0
12	2.616488236704969E-4	0.0	0.0	15.562959248195714	0.0
13	2.616488236704969E-4	0.0	0.0	16.287464840939318	0.0
14	2.616488236704969E-4	0.0	0.0	16.529053921461745	0.0
15	2.616488236704969E-4	0.0	0.0	16.89675773499335	0.0
16	2.616488236704969E-4	0.0	0.0	17.76839718291433	0.0
17	2.616488236704969E-4	0.0	0.0	19.11754573403397	0.0
18	2.616488236704969E-4	0.0	0.0	20.556090966574363	0.0
19	2.616488236704969E-4	0.0	0.0	21.657894163050827	0.0
20	2.616488236704969E-4	0.0	0.0	22.647188365348974	0.0
21	2.616488236704969E-4	0.0	0.0	24.13099884438436	0.0
22	2.616488236704969E-4	0.0	0.0	25.68859429169483	0.0
23	2.616488236704969E-4	0.0	0.0	27.254213636264527	0.0
24	2.616488236704969E-4	0.0	0.0	28.376948738634628	0.0
25	2.616488236704969E-4	0.0	0.0	29.34775309072673	0.0
26	2.616488236704969E-4	0.0	0.0	30.19270435863332	0.0
27	2.616488236704969E-4	0.0	0.0	31.032422650066504	0.0
28	2.616488236704969E-4	0.0	0.0	31.913655888188735	0.0
29	2.616488236704969E-4	0.0	0.0	32.87015677125352	0.0
30	2.616488236704969E-4	0.0	0.0	33.834071037655626	0.0
31	2.616488236704969E-4	0.0	0.0	34.78673440463991	0.0
32	3.488650982273292E-4	0.0	0.0	35.692911497285394	0.0
33	3.488650982273292E-4	0.0	0.0	36.5986525085581	0.0
34	3.488650982273292E-4	0.0	0.0	37.4718618494211	0.0
35	3.488650982273292E-4	0.0	0.0	38.32466258203781	0.0
36	3.488650982273292E-4	0.0	0.0	39.186010509561086	0.0
37	4.360813727841615E-4	0.0	0.0	40.04875389747727	0.0
38	4.360813727841615E-4	0.0	0.0	40.90024638597562	0.0
39	4.360813727841615E-4	0.0	0.0	41.708915683666575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	20	7.033278E-4	45.000004	2
TAACGTG	20	7.033278E-4	45.000004	18
CGCCTCG	20	7.033278E-4	45.000004	15
TTCGCCC	20	7.033278E-4	45.000004	13
CAATACG	20	7.033278E-4	45.000004	18
ACGACAG	20	7.033278E-4	45.000004	10
GCGACGG	50	2.1827873E-11	44.999996	2
CGCGACA	25	3.890801E-5	44.999996	30
GAGCGTA	30	2.1654978E-6	44.999996	9
TGCGACG	50	2.1827873E-11	44.999996	1
CTACCGG	25	3.890801E-5	44.999996	2
TCGACCA	25	3.890801E-5	44.999996	20
GCGTCAT	25	3.890801E-5	44.999996	37
TCGCGAC	25	3.890801E-5	44.999996	29
CGTTTTT	2100	0.0	41.89286	1
TACGGGT	60	3.6379788E-12	41.249996	4
ACTGCGC	110	0.0	40.909092	19
TTCCGCG	110	0.0	40.909092	1
CGCTAAA	110	0.0	40.909092	23
GCGCGAC	215	0.0	40.81395	9
>>END_MODULE