SRR2934372.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934372.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018426
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3498	0.3434711996747923	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	2806	0.2755232093446161	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2636	0.2588307839744861	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2539	0.2493062824397649	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2536	0.24901171022735083	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1555	0.15268659676795368	No Hit
TCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	1212	0.11900717381527964	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.032909E-4	45.0	27
AAATCCG	20	7.032909E-4	45.0	37
CGACGGC	20	7.032909E-4	45.0	20
GACCGAC	20	7.032909E-4	45.0	9
TAAGTCG	20	7.032909E-4	45.0	44
TACGGGT	25	3.890491E-5	45.0	4
CGCGACA	25	3.890491E-5	45.0	25
TAACGCC	20	7.032909E-4	45.0	12
GTTACGT	20	7.032909E-4	45.0	22
TATTACG	20	7.032909E-4	45.0	1
ACGTAAC	20	7.032909E-4	45.0	22
ACATACG	105	0.0	45.0	17
AATCGGG	20	7.032909E-4	45.0	21
ATCGACT	20	7.032909E-4	45.0	14
TATCCGG	35	1.211738E-7	45.0	2
TCATACG	30	2.1652577E-6	44.999996	31
CGACTCC	30	2.1652577E-6	44.999996	28
TTTCGCG	335	0.0	44.328358	1
CGAATAT	120	0.0	43.124996	14
TACGAAA	110	0.0	42.954544	20