##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934372.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018426
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18402220681719	33.0	31.0	34.0	30.0	34.0
2	32.379197899503744	34.0	31.0	34.0	30.0	34.0
3	32.44697700176547	34.0	31.0	34.0	30.0	34.0
4	35.94276756485007	37.0	35.0	37.0	35.0	37.0
5	35.89242811947064	37.0	35.0	37.0	35.0	37.0
6	35.53272795470658	37.0	35.0	37.0	33.0	37.0
7	35.85435858864562	37.0	35.0	37.0	35.0	37.0
8	35.945441298631415	37.0	35.0	37.0	35.0	37.0
9	37.72396030737629	39.0	38.0	39.0	35.0	39.0
10	37.266113590972736	39.0	37.0	39.0	34.0	39.0
11	37.135644612372424	39.0	37.0	39.0	34.0	39.0
12	36.69248919410934	39.0	35.0	39.0	33.0	39.0
13	36.46899529273605	39.0	35.0	39.0	33.0	39.0
14	37.177235263043166	40.0	36.0	41.0	31.0	41.0
15	37.354852488055094	40.0	35.0	41.0	32.0	41.0
16	37.545780449438645	40.0	35.0	41.0	33.0	41.0
17	37.55201556126807	39.0	35.0	41.0	33.0	41.0
18	37.53687847717949	39.0	35.0	41.0	33.0	41.0
19	37.55013815436762	39.0	35.0	41.0	33.0	41.0
20	37.4437877666124	39.0	35.0	41.0	32.0	41.0
21	37.29508182234154	39.0	35.0	41.0	32.0	41.0
22	37.47528440947109	39.0	35.0	41.0	33.0	41.0
23	37.4689697631443	39.0	35.0	41.0	33.0	41.0
24	37.42296936645372	39.0	35.0	41.0	33.0	41.0
25	37.35536602561207	39.0	35.0	41.0	33.0	41.0
26	37.30848191228425	39.0	35.0	41.0	33.0	41.0
27	37.251785598560915	39.0	35.0	41.0	33.0	41.0
28	37.1333312385976	39.0	35.0	41.0	32.0	41.0
29	37.04841687073975	39.0	35.0	41.0	32.0	41.0
30	37.0013550321771	39.0	35.0	41.0	32.0	41.0
31	36.86647139802008	39.0	35.0	41.0	31.0	41.0
32	36.76659767130847	39.0	35.0	41.0	31.0	41.0
33	36.739694391148696	39.0	35.0	41.0	31.0	41.0
34	36.522883351367696	39.0	35.0	41.0	31.0	41.0
35	36.428357092218775	39.0	35.0	41.0	30.0	41.0
36	36.30796935663465	38.0	35.0	40.0	30.0	41.0
37	36.25348233450442	38.0	35.0	40.0	30.0	41.0
38	36.176173821171105	38.0	35.0	40.0	30.0	41.0
39	36.12047316152573	38.0	35.0	40.0	30.0	41.0
40	35.92349665071394	38.0	35.0	40.0	29.0	41.0
41	35.78253206418532	38.0	35.0	40.0	29.0	41.0
42	35.77018556085567	38.0	35.0	40.0	29.0	41.0
43	35.745661442264826	38.0	35.0	40.0	29.0	41.0
44	35.590304057437656	38.0	34.0	40.0	29.0	41.0
45	35.65231052624344	37.0	34.0	40.0	29.0	41.0
46	35.57987914684032	37.0	34.0	40.0	29.0	41.0
47	35.33924703414878	37.0	34.0	40.0	29.0	41.0
48	35.24200383729402	37.0	34.0	40.0	28.0	41.0
49	35.251640276269455	37.0	34.0	40.0	28.0	41.0
50	35.124962442042914	36.0	34.0	40.0	28.0	41.0
51	34.13462146488797	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	25.0
11	24.0
12	16.0
13	18.0
14	29.0
15	55.0
16	97.0
17	151.0
18	290.0
19	504.0
20	883.0
21	1363.0
22	2237.0
23	3056.0
24	4498.0
25	6382.0
26	8554.0
27	10739.0
28	12854.0
29	15582.0
30	19731.0
31	25729.0
32	34725.0
33	50710.0
34	90193.0
35	86155.0
36	84501.0
37	126146.0
38	200460.0
39	232644.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.93416311052546	4.856612066070584	53.05599032232091	17.153234501083045
2	21.16884290071149	4.643243593545334	51.76704051153447	22.42087299420871
3	22.55804545445619	4.984652787733228	50.43194105413649	22.025360703674103
4	20.3797821343917	5.333819050181359	49.13356493255278	25.15283388287416
5	18.90220791692278	6.501797872403101	48.63092654743693	25.96506766323719
6	22.816679856955734	6.464976345851343	52.08105448996785	18.63728930722507
7	83.75915383150077	2.63465386783134	8.904525218327104	4.701667082340789
8	84.87597527949994	3.0880005027365756	7.3775610599100965	4.658463157853393
9	79.64712212767544	5.03492644531856	9.649891106472145	5.668060320533844
10	44.49925669611734	24.240641931765293	16.667288541337317	14.592812830780048
11	34.87469879991281	21.823676928907943	23.46896092597793	19.832663345201322
12	31.537392014736465	18.9347090510258	28.75211355562407	20.775785378613666
13	28.880154277286714	20.717067317605796	29.960350580209067	20.44242782489842
14	22.09271954957945	22.628840976173038	33.46850924858556	21.80993022566195
15	19.90355705765564	22.28949378747204	35.761753922229005	22.045195232643312
16	26.808526098116115	21.813170519998508	29.739225039423584	21.639078342461797
17	27.73741047459511	21.804431544363556	26.616465015622147	23.84169296541919
18	29.46821860400265	21.463218731650606	28.767824073619487	20.30073859072726
19	27.694108359370244	23.692246662987788	26.54694597349243	22.06669900414954
20	26.944323888038994	27.688511487334377	26.445220369472107	18.92194425515452
21	26.360285381559383	25.708200694012135	29.851751624565754	18.07976229986273
22	24.84068552845273	21.06436795604197	31.233786254475042	22.861160261030257
23	23.320791103133658	23.16633707309122	31.040154120181533	22.472717703593585
24	24.869357223794363	24.514397707835425	28.53579936097468	22.08044570739553
25	26.321401849520733	23.68674798168939	25.43827435670338	24.553575812086496
26	22.069939298486098	23.920736509083625	26.892675560129064	27.116648632301217
27	21.01861107238032	22.430692067955846	31.659639482888302	24.89105737677553
28	18.238929485303792	24.727766180360675	29.502781743592564	27.53052259074297
29	20.702436897722563	25.34283295988123	28.92355458324905	25.031175559147158
30	24.209319086511933	22.660949347326167	30.222323467782637	22.907408098379264
31	24.89714520249876	22.870586571827506	29.833095384446196	22.39917284122754
32	26.652402825536665	22.720060171283922	27.390502599108824	23.237034404070595
33	26.39033174722562	21.444267919318634	27.212286410598313	24.95311392285743
34	21.081158572149572	22.092326786629563	32.616606410284106	24.209908230936758
35	21.300418488923103	24.930922816188904	30.04705300139627	23.72160569349172
36	24.093061253345848	24.886246030639438	27.439990730794385	23.58070198522033
37	21.612763224819474	26.942163691814624	27.63705954089939	23.80801354246651
38	24.129686398422663	25.650268158904034	25.916266866713926	24.303778575959374
39	22.712597675236097	22.87215762362705	26.03704147380369	28.37820322733316
40	25.998550704714923	21.592437742162907	27.92161629809137	24.4873952550308
41	22.2676954437534	21.714783401052212	27.5290497296809	28.488471425513488
42	21.364929803441782	22.574934261301262	29.156855775480988	26.903280159775967
43	21.843609648614624	20.58519715718177	29.494239149432556	28.076954044771046
44	22.464076918696104	21.447900976605077	28.422880012882622	27.665142091816197
45	22.15025932173766	20.27255784907298	27.38539668076031	30.191786148429046
46	22.635910709270973	21.146062649618138	29.576130224483666	26.641896416627226
47	19.02111690000059	22.98026562558301	32.34363616011374	25.65498131430266
48	19.70855025303753	24.762525701425535	29.98941503850059	25.539509007036347
49	23.650810171774875	21.036383595862635	30.57109696728088	24.741709265081607
50	21.952404985732883	21.842725931977387	28.83380824920024	27.371060833089494
51	20.69399249430003	22.02968109612284	27.446373128730023	29.82995328084711
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	191.0
1	775.0
2	1359.0
3	2905.0
4	4451.0
5	3064.0
6	1677.0
7	1693.5
8	1710.0
9	1729.0
10	1748.0
11	1798.0
12	1848.0
13	1778.0
14	1708.0
15	1702.0
16	1696.0
17	1689.5
18	1683.0
19	1766.0
20	1849.0
21	2364.5
22	2880.0
23	2834.5
24	2789.0
25	3322.0
26	4869.0
27	5883.0
28	7500.0
29	9117.0
30	10262.5
31	11408.0
32	13814.5
33	16221.0
34	17879.0
35	19537.0
36	21924.0
37	24311.0
38	26241.0
39	28171.0
40	33170.5
41	38170.0
42	41701.5
43	45233.0
44	51230.0
45	57227.0
46	65865.5
47	74504.0
48	87901.5
49	101299.0
50	101698.0
51	102097.0
52	91908.0
53	81719.0
54	77334.5
55	72950.0
56	70316.0
57	67682.0
58	64812.0
59	61942.0
60	55542.5
61	49143.0
62	43337.0
63	37531.0
64	32868.5
65	28206.0
66	24275.5
67	20345.0
68	17148.0
69	13951.0
70	11321.0
71	8691.0
72	7392.5
73	6094.0
74	4925.5
75	2871.0
76	1985.0
77	1524.0
78	1063.0
79	728.0
80	393.0
81	311.0
82	229.0
83	138.0
84	47.0
85	37.5
86	28.0
87	28.0
88	28.0
89	22.0
90	16.0
91	9.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1018426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.367600149193027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57501975992078	18.1568648194026
2	11.50990579347481	5.839573758474984
3	4.473889042266896	3.4047548500824822
4	2.2984685566835075	2.332265252057601
5	1.379072019073424	1.7491873778397455
6	0.9068574383844877	1.380287813355548
7	0.6318483508519711	1.121993342353788
8	0.4895515415535419	0.9934998206841043
9	0.3949588487041587	0.901724233438145
>10	4.652634616404181	29.469958803055885
>50	1.5417612866502277	26.212170429540453
>100	0.13820257198201058	5.903165936392598
>500	0.005089613132481972	0.8678263728961016
>1k	0.0027405609174902925	1.666727190425999
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3498	0.3434711996747923	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	2806	0.2755232093446161	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2636	0.2588307839744861	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2539	0.2493062824397649	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2536	0.24901171022735083	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1555	0.15268659676795368	No Hit
TCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	1212	0.11900717381527964	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9457221241405856E-4	0.0	0.0	0.29575050126371477	0.0
2	2.9457221241405856E-4	0.0	0.0	1.2386761532011161	0.0
3	2.9457221241405856E-4	0.0	0.0	1.8006217437496685	0.0
4	2.9457221241405856E-4	0.0	0.0	2.4080296457474573	0.0
5	2.9457221241405856E-4	0.0	0.0	3.4209652935019332	0.0
6	2.9457221241405856E-4	0.0	0.0	4.099070526479096	0.0
7	2.9457221241405856E-4	0.0	0.0	4.691062482693883	0.0
8	2.9457221241405856E-4	0.0	0.0	5.5659419535636365	0.0
9	2.9457221241405856E-4	0.0	0.0	6.004167214898284	0.0
10	2.9457221241405856E-4	0.0	0.0	6.810705932487976	0.0
11	2.9457221241405856E-4	0.0	0.0	8.322450526596924	0.0
12	2.9457221241405856E-4	0.0	0.0	9.66697629479216	0.0
13	2.9457221241405856E-4	0.0	0.0	10.144968804802705	0.0
14	2.9457221241405856E-4	0.0	0.0	10.328487293136664	0.0
15	2.9457221241405856E-4	0.0	0.0	10.59890458413277	0.0
16	2.9457221241405856E-4	0.0	0.0	11.21652432282758	0.0
17	2.9457221241405856E-4	0.0	0.0	12.012949394457722	0.0
18	2.9457221241405856E-4	0.0	0.0	12.926516015891188	0.0
19	2.9457221241405856E-4	0.0	0.0	13.526265040366212	0.0
20	2.9457221241405856E-4	0.0	0.0	14.223812039362702	0.0
21	2.9457221241405856E-4	0.0	0.0	15.112732785690861	0.0
22	2.9457221241405856E-4	0.0	0.0	15.988594163935328	0.0
23	2.9457221241405856E-4	0.0	0.0	16.813003595744807	0.0
24	2.9457221241405856E-4	0.0	0.0	17.40902137219592	0.0
25	2.9457221241405856E-4	0.0	0.0	17.95653292433618	0.0
26	2.9457221241405856E-4	0.0	0.0	18.466928377712275	0.0
27	2.9457221241405856E-4	0.0	0.0	19.07404170749765	0.0
28	2.9457221241405856E-4	0.0	0.0	19.6346126277216	0.0
29	2.9457221241405856E-4	0.0	0.0	20.298283822290475	0.0
30	2.9457221241405856E-4	0.0	0.0	21.023029655566532	0.0
31	2.9457221241405856E-4	0.0	0.0	21.53381787189251	0.0
32	2.9457221241405856E-4	0.0	0.0	22.042151319781702	0.0
33	2.9457221241405856E-4	0.0	0.0	22.587404484960125	0.0
34	2.9457221241405856E-4	0.0	0.0	23.197070773919755	0.0
35	2.9457221241405856E-4	0.0	0.0	23.866829794211853	0.0
36	2.9457221241405856E-4	0.0	0.0	24.434666829008687	0.0
37	2.9457221241405856E-4	0.0	0.0	25.01124283944047	0.0
38	2.9457221241405856E-4	0.0	0.0	25.75886711454735	0.0
39	2.9457221241405856E-4	0.0	0.0	27.169377058323334	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.032909E-4	45.0	27
AAATCCG	20	7.032909E-4	45.0	37
CGACGGC	20	7.032909E-4	45.0	20
GACCGAC	20	7.032909E-4	45.0	9
TAAGTCG	20	7.032909E-4	45.0	44
TACGGGT	25	3.890491E-5	45.0	4
CGCGACA	25	3.890491E-5	45.0	25
TAACGCC	20	7.032909E-4	45.0	12
GTTACGT	20	7.032909E-4	45.0	22
TATTACG	20	7.032909E-4	45.0	1
ACGTAAC	20	7.032909E-4	45.0	22
ACATACG	105	0.0	45.0	17
AATCGGG	20	7.032909E-4	45.0	21
ATCGACT	20	7.032909E-4	45.0	14
TATCCGG	35	1.211738E-7	45.0	2
TCATACG	30	2.1652577E-6	44.999996	31
CGACTCC	30	2.1652577E-6	44.999996	28
TTTCGCG	335	0.0	44.328358	1
CGAATAT	120	0.0	43.124996	14
TACGAAA	110	0.0	42.954544	20
>>END_MODULE