FastQCFastQC Report
Sat 14 Jan 2017
SRR2934369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087046
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC132970.6371206001209365No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG125180.599795117117687No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC120530.5775148223853235No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC49380.23660235567400048TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.2306130291330426No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC46710.22380915418251443TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT43350.2077098444404196TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC39920.19127513241203117TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT31360.15026022425955154No Hit
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT30150.1444625561679043No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT30110.14427089771859367No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC29520.14144393559126153No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA23940.1147075819124255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1100.045.08
CGATCGC207.034611E-445.034
ACGCGCC253.891904E-545.044
GCGATAT603.6379788E-1241.2499969
TACGGGA5000.040.954
TTTCGCG5500.040.51
TATCGCG451.929584E-840.01
TTGGGAT103550.039.6112985
GGGCGAT34600.039.60267
GGCCGAT5450.039.2201848
TTTGGGA150650.039.1304324
TGGGCGA24300.039.0740746
CGTTTTT28900.038.9273381
CTTTGCG8250.038.7272721
CGCATCG700.038.5714321
CGCGCCA356.250739E-638.5714320
ACTACGC356.250739E-638.5714321
CGATGAA7700.038.57142619
GCGATTG6750.038.3333329
TTGGGAC54450.038.223145
Produced by FastQC (version 0.11.5)