##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087046
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.226868502179634	33.0	31.0	34.0	30.0	34.0
2	32.4128231960388	34.0	31.0	34.0	30.0	34.0
3	32.48763323855823	34.0	31.0	34.0	30.0	34.0
4	35.978180164692105	37.0	35.0	37.0	35.0	37.0
5	35.93409057586656	37.0	35.0	37.0	35.0	37.0
6	35.51094369745564	37.0	35.0	37.0	33.0	37.0
7	35.91163922596819	37.0	35.0	37.0	35.0	37.0
8	35.95353672127974	37.0	35.0	37.0	35.0	37.0
9	37.766456034030874	39.0	38.0	39.0	35.0	39.0
10	37.291346716842845	39.0	37.0	39.0	34.0	39.0
11	37.17300049927026	39.0	37.0	39.0	34.0	39.0
12	37.078764914620955	39.0	37.0	39.0	33.0	39.0
13	37.0365257881235	39.0	37.0	39.0	33.0	39.0
14	38.22737160560908	40.0	38.0	41.0	33.0	41.0
15	38.252062484487645	40.0	38.0	41.0	33.0	41.0
16	38.308901193361336	40.0	38.0	41.0	34.0	41.0
17	38.19834397516873	40.0	37.0	41.0	33.0	41.0
18	38.177437871517924	40.0	37.0	41.0	33.0	41.0
19	38.20669070063621	40.0	37.0	41.0	34.0	41.0
20	38.11603146265104	40.0	37.0	41.0	34.0	41.0
21	38.026983113932324	40.0	37.0	41.0	33.0	41.0
22	38.099738577875144	40.0	37.0	41.0	34.0	41.0
23	38.013725619847385	40.0	37.0	41.0	33.0	41.0
24	37.97242897377441	40.0	37.0	41.0	33.0	41.0
25	37.927946485127784	40.0	37.0	41.0	33.0	41.0
26	37.86910398716655	40.0	37.0	41.0	33.0	41.0
27	37.77934698133151	40.0	36.0	41.0	33.0	41.0
28	37.647156315672966	40.0	36.0	41.0	33.0	41.0
29	37.55750711771566	40.0	36.0	41.0	33.0	41.0
30	37.56511452071492	40.0	36.0	41.0	33.0	41.0
31	37.543329663074026	40.0	36.0	41.0	33.0	41.0
32	37.394916547119706	40.0	36.0	41.0	32.0	41.0
33	37.38177740212722	40.0	36.0	41.0	32.0	41.0
34	37.226233633566295	40.0	36.0	41.0	32.0	41.0
35	37.1918961057878	40.0	36.0	41.0	31.0	41.0
36	37.06497652663142	39.0	35.0	41.0	31.0	41.0
37	37.06366941600712	39.0	35.0	41.0	31.0	41.0
38	36.993513319783084	39.0	35.0	41.0	31.0	41.0
39	36.93822656520268	39.0	35.0	41.0	31.0	41.0
40	36.82345094454075	39.0	35.0	41.0	31.0	41.0
41	36.72638168971839	39.0	35.0	41.0	31.0	41.0
42	36.72398404251751	39.0	35.0	41.0	31.0	41.0
43	36.68450863085912	39.0	35.0	41.0	31.0	41.0
44	36.58386829997997	39.0	35.0	41.0	30.0	41.0
45	36.600271867510344	39.0	35.0	41.0	30.0	41.0
46	36.506456494011154	39.0	35.0	40.0	30.0	41.0
47	36.304303786308495	39.0	35.0	40.0	30.0	41.0
48	36.242482436898854	39.0	35.0	40.0	30.0	41.0
49	36.26514269450697	39.0	35.0	40.0	30.0	41.0
50	36.168957943428175	38.0	35.0	40.0	30.0	41.0
51	35.32019083431798	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	34.0
10	23.0
11	41.0
12	39.0
13	35.0
14	47.0
15	62.0
16	101.0
17	181.0
18	368.0
19	683.0
20	1350.0
21	2433.0
22	4093.0
23	6008.0
24	7946.0
25	10572.0
26	14161.0
27	17519.0
28	20854.0
29	25039.0
30	31261.0
31	40576.0
32	53896.0
33	74330.0
34	120306.0
35	148104.0
36	164748.0
37	248947.0
38	449277.0
39	643796.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.20699304184	4.204507231752438	53.75094751145878	15.837552214948783
2	19.116444965755427	7.826420692212821	52.24935147572215	20.80778286630961
3	20.31114790953338	7.906581838637002	50.96490446305448	20.817365788775138
4	18.49058429953149	4.628743209301568	53.25143767794289	23.629234813224052
5	20.83725035289112	5.345689553560391	49.24045756538188	24.576602528166607
6	20.944770742954397	8.369245335272916	52.377091832187695	18.30889208958499
7	83.62767279686216	1.8610514574187633	10.596412345487353	3.914863400231715
8	85.04546617563771	4.757968918749275	6.334311749717064	3.862253155895941
9	80.07298353749749	5.172669888445199	7.6409432278924365	7.1134033461648665
10	36.948490833455516	36.79765563384803	14.120244594513009	12.133608938183443
11	26.260609493034654	24.75139503393792	30.46803951613908	18.51995595688835
12	26.085433670364715	21.31433614783766	31.865469184675373	20.734760997122248
13	24.312449270404198	21.71418358771201	33.3034825298532	20.669884612030593
14	18.98065495441883	27.134044961155624	30.79112774706451	23.094172337361034
15	17.49391244850377	26.594334767896825	35.38661821541068	20.525134568188722
16	20.57726566640122	26.1908937320979	32.01127334998845	21.220567251512428
17	20.37434728319357	25.524066072333817	31.512578064882135	22.589008579590484
18	20.482011417093826	25.927938339643685	30.93467992559819	22.655370317664296
19	21.800717377575772	27.47778438999428	29.546162374954843	21.175335857475112
20	25.872021987057302	26.19209159740609	30.13354760747966	17.802338808056938
21	24.915454666547838	27.438206920211627	28.74019068099122	18.906147732249313
22	22.087438417744508	23.596413303779602	30.939615130667942	23.376533147807955
23	21.73445146872661	27.238594645254583	30.242170033626476	20.78478385239233
24	22.131471946473628	24.58752705977731	30.32055833939453	22.96044265435453
25	21.26110301354163	28.132250079777833	27.232844891775265	23.37380201490528
26	21.042756125164466	28.09593080363346	28.31178613216958	22.54952693903249
27	21.71542936763253	28.13914978395301	29.021832772253227	21.123588076161234
28	19.20106217112608	28.535499457127443	31.225234134753137	21.038204236993337
29	21.451755255993397	26.242306111125487	30.549973503219384	21.755965129661732
30	21.721370779561163	25.33609704817239	31.990143005951953	20.952389166314493
31	22.990245543222336	26.2799190818027	27.542229543574987	23.187605831399978
32	22.423271935549096	27.88501068016709	29.50131429781615	20.19040308646767
33	23.08732054779818	25.170791635641955	28.649057088344005	23.09283072821586
34	21.571973018323508	26.483652013419924	29.64946627913328	22.29490868912329
35	20.502279298108427	25.07582487400853	31.088964977293266	23.33293085058978
36	20.741612786685103	27.653391444175163	30.04399519703926	21.561000572100472
37	21.485822545358367	26.47799808916526	31.341283325810736	20.694896039665633
38	22.617565688537773	25.57475973217648	29.36748878558498	22.440185793700763
39	22.380100869841872	24.10205620767343	30.11730455390059	23.400538368584115
40	22.66231793645181	23.67949724155577	31.39307902173694	22.26510580025548
41	18.953631113066027	25.616253786452237	31.6176548097167	23.812460290765035
42	20.622880377337154	25.694067116872365	31.488860331779943	22.19419217401054
43	21.35496773909152	25.540261211300564	29.971692047036818	23.133079002571098
44	21.826016292884777	25.12551232699231	29.67289652456151	23.3755748555614
45	21.91044183980612	23.603025520280816	28.751498529500548	25.735034110412514
46	22.259212302939176	25.51453106448061	29.1180932284195	23.108163404160713
47	19.7839434300921	25.461393759409233	32.29296335586279	22.461699454635884
48	20.843239679432077	24.242781424079777	31.22216759956417	23.69181129692398
49	20.665332723859464	23.284968323649792	32.77479269742977	23.27490625506098
50	20.490875620374442	23.258615286869574	31.04823755681475	25.20227153594123
51	19.232973302936305	23.343519979914195	29.658234653189247	27.765272063960257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	416.0
1	1506.5
2	2597.0
3	5993.0
4	9389.0
5	6544.5
6	3700.0
7	3610.0
8	3520.0
9	3628.5
10	3737.0
11	3752.0
12	3767.0
13	3669.0
14	3571.0
15	3667.0
16	3763.0
17	4122.5
18	4482.0
19	5089.0
20	5696.0
21	6965.5
22	8235.0
23	9855.0
24	11475.0
25	13781.5
26	21136.5
27	26185.0
28	31085.0
29	35985.0
30	41317.5
31	46650.0
32	51968.0
33	57286.0
34	68263.5
35	79241.0
36	82060.0
37	84879.0
38	91327.0
39	97775.0
40	106994.5
41	116214.0
42	124668.0
43	133122.0
44	174007.5
45	214893.0
46	206883.5
47	198874.0
48	189461.5
49	180049.0
50	162643.5
51	145238.0
52	128719.5
53	112201.0
54	103474.0
55	94747.0
56	90932.0
57	87117.0
58	86404.5
59	85692.0
60	79332.0
61	72972.0
62	63019.0
63	53066.0
64	43640.0
65	34214.0
66	27066.5
67	19919.0
68	15840.0
69	11761.0
70	9869.0
71	7977.0
72	6139.0
73	4301.0
74	3848.5
75	2577.5
76	1759.0
77	1198.0
78	637.0
79	467.0
80	297.0
81	207.0
82	117.0
83	73.5
84	30.0
85	18.5
86	7.0
87	4.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2087046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.58107609417023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.20215330742771	15.85240165809629
2	12.061313110937872	5.447148583074022
3	4.953468029977418	3.355639155448787
4	2.4817379615103228	2.24161255018622
5	1.4376460579438357	1.6231797515456807
6	0.8946681206855397	1.2121541347337068
7	0.647119520348636	1.022885859171084
8	0.4585298031475402	0.82832771010556
9	0.3632522633438573	0.7382364299952483
>10	4.340979064342528	25.365847715723504
>50	1.953375701268063	30.084042923300792
>100	0.19371694807012924	6.987479709946371
>500	0.007525069372862743	1.1168872558967575
>1k	0.003870035677472267	2.2856742064926516
>5k	0.0	0.0
>10k+	6.450059462453779E-4	1.8384823562833477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	13297	0.6371206001209365	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	12518	0.599795117117687	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	12053	0.5775148223853235	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4938	0.23660235567400048	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4813	0.2306130291330426	No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4671	0.22380915418251443	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	4335	0.2077098444404196	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3992	0.19127513241203117	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT	3136	0.15026022425955154	No Hit
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3015	0.1444625561679043	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	3011	0.14427089771859367	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC	2952	0.14144393559126153	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA	2394	0.1147075819124255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9165844931065247E-4	0.0	0.0	0.22227588658802921	0.0
2	1.9165844931065247E-4	0.0	0.0	0.9383597678249546	0.0
3	1.9165844931065247E-4	0.0	0.0	1.3252702623708341	0.0
4	1.9165844931065247E-4	0.0	0.0	1.9640199593109111	0.0
5	1.9165844931065247E-4	0.0	0.0	3.289961026254333	0.0
6	1.9165844931065247E-4	0.0	0.0	3.8522869165317872	0.0
7	1.9165844931065247E-4	0.0	0.0	4.486772212974702	0.0
8	1.9165844931065247E-4	0.0	0.0	5.349426893321949	0.0
9	1.9165844931065247E-4	0.0	0.0	5.70064100168372	0.0
10	1.9165844931065247E-4	0.0	0.0	7.023036387314894	0.0
11	1.9165844931065247E-4	0.0	0.0	8.288700871950114	0.0
12	2.3957306163831558E-4	0.0	0.0	10.185448715552987	0.0
13	2.3957306163831558E-4	0.0	0.0	10.57681526904534	0.0
14	2.3957306163831558E-4	0.0	0.0	10.747726691218114	0.0
15	2.3957306163831558E-4	0.0	0.0	11.06928165454906	0.0
16	2.3957306163831558E-4	0.0	0.0	11.542773853571028	0.0
17	2.3957306163831558E-4	0.0	0.0	12.20294138222157	0.0
18	2.874876739659787E-4	0.0	0.0	12.926212455307645	0.0
19	2.874876739659787E-4	0.0	0.0	13.654370818851142	0.0
20	3.3540228629364184E-4	0.0	0.0	14.235527151773368	0.0
21	3.3540228629364184E-4	0.0	0.0	15.010977237684267	0.0
22	3.3540228629364184E-4	0.0	0.0	15.912826070915543	0.0
23	3.3540228629364184E-4	0.0	0.0	16.72358922611193	0.0
24	3.3540228629364184E-4	0.0	0.0	17.31634089521745	0.0
25	4.3123151094896805E-4	0.0	0.0	17.837220645831476	0.0
26	4.7914612327663115E-4	0.0	0.0	18.3040527137399	0.0
27	4.7914612327663115E-4	0.0	0.0	18.776778278964624	0.0
28	4.7914612327663115E-4	0.0	0.0	19.274131954925767	0.0
29	4.7914612327663115E-4	0.0	0.0	19.80325301886015	0.0
30	5.270607356042943E-4	0.0	0.0	20.42508885764856	0.0
31	5.270607356042943E-4	0.0	0.0	20.981090019098765	0.0
32	5.270607356042943E-4	0.0	0.0	21.51284638671117	0.0
33	5.270607356042943E-4	0.0	0.0	22.04024252460176	0.0
34	5.270607356042943E-4	0.0	0.0	22.54641248923119	0.0
35	5.270607356042943E-4	0.0	0.0	23.110798707838736	0.0
36	5.270607356042943E-4	0.0	0.0	23.66818939304644	0.0
37	5.270607356042943E-4	0.0	0.0	24.23894825509356	0.0
38	5.270607356042943E-4	0.0	0.0	24.81842757658432	0.0
39	5.270607356042943E-4	0.0	0.0	25.375195371831765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	110	0.0	45.0	8
CGATCGC	20	7.034611E-4	45.0	34
ACGCGCC	25	3.891904E-5	45.0	44
GCGATAT	60	3.6379788E-12	41.249996	9
TACGGGA	500	0.0	40.95	4
TTTCGCG	550	0.0	40.5	1
TATCGCG	45	1.929584E-8	40.0	1
TTGGGAT	10355	0.0	39.611298	5
GGGCGAT	3460	0.0	39.6026	7
GGCCGAT	545	0.0	39.220184	8
TTTGGGA	15065	0.0	39.130432	4
TGGGCGA	2430	0.0	39.074074	6
CGTTTTT	2890	0.0	38.927338	1
CTTTGCG	825	0.0	38.727272	1
CGCATCG	70	0.0	38.57143	21
CGCGCCA	35	6.250739E-6	38.57143	20
ACTACGC	35	6.250739E-6	38.57143	21
CGATGAA	770	0.0	38.571426	19
GCGATTG	675	0.0	38.333332	9
TTGGGAC	5445	0.0	38.22314	5
>>END_MODULE