##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2058014
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.255072122930166	33.0	31.0	34.0	30.0	34.0
2	32.44688714459669	34.0	31.0	34.0	31.0	34.0
3	32.5101306405107	34.0	31.0	34.0	31.0	34.0
4	35.982499633141465	37.0	35.0	37.0	35.0	37.0
5	35.93539839865035	37.0	35.0	37.0	35.0	37.0
6	35.56254427812444	37.0	35.0	37.0	33.0	37.0
7	35.89397934124841	37.0	35.0	37.0	35.0	37.0
8	35.98854964057582	37.0	35.0	37.0	35.0	37.0
9	37.775796471744115	39.0	38.0	39.0	35.0	39.0
10	37.28149274008826	39.0	37.0	39.0	34.0	39.0
11	37.16759215437796	39.0	37.0	39.0	34.0	39.0
12	36.85733381794293	39.0	35.0	39.0	33.0	39.0
13	36.72711896031805	39.0	35.0	39.0	33.0	39.0
14	37.599586300190374	40.0	37.0	41.0	32.0	41.0
15	37.72861506287129	40.0	36.0	41.0	33.0	41.0
16	37.85998103025538	40.0	36.0	41.0	33.0	41.0
17	37.855924692446216	40.0	36.0	41.0	33.0	41.0
18	37.81652845899008	40.0	36.0	41.0	33.0	41.0
19	37.83820080912958	40.0	36.0	41.0	33.0	41.0
20	37.75324268931115	40.0	36.0	41.0	33.0	41.0
21	37.60623640072419	40.0	35.0	41.0	33.0	41.0
22	37.72813450248638	40.0	35.0	41.0	33.0	41.0
23	37.7160514943047	39.0	35.0	41.0	33.0	41.0
24	37.657624292157394	39.0	35.0	41.0	33.0	41.0
25	37.56320073624378	39.0	35.0	41.0	33.0	41.0
26	37.51080264760104	39.0	35.0	41.0	33.0	41.0
27	37.428238583410995	39.0	35.0	41.0	33.0	41.0
28	37.29543870935766	39.0	35.0	41.0	32.0	41.0
29	37.192787804164595	39.0	35.0	41.0	32.0	41.0
30	37.127350931529136	39.0	35.0	41.0	32.0	41.0
31	37.00433767700317	39.0	35.0	41.0	31.0	41.0
32	36.879575649145245	39.0	35.0	41.0	31.0	41.0
33	36.82855024309844	39.0	35.0	41.0	31.0	41.0
34	36.6205171587754	39.0	35.0	41.0	30.0	41.0
35	36.55196028792807	39.0	35.0	41.0	30.0	41.0
36	36.37859071901357	39.0	35.0	41.0	30.0	41.0
37	36.28440428490768	39.0	35.0	41.0	30.0	41.0
38	36.19366826464737	39.0	35.0	41.0	30.0	41.0
39	36.155087380357955	39.0	35.0	40.0	30.0	41.0
40	36.01422779436874	38.0	35.0	40.0	29.0	41.0
41	35.873149065069526	38.0	35.0	40.0	29.0	41.0
42	35.81328989987434	38.0	35.0	40.0	29.0	41.0
43	35.752580400327695	38.0	35.0	40.0	29.0	41.0
44	35.607763115314086	38.0	34.0	40.0	28.0	41.0
45	35.638072918843115	38.0	34.0	40.0	28.0	41.0
46	35.558134201225066	38.0	34.0	40.0	28.0	41.0
47	35.32373929429051	37.0	34.0	40.0	27.0	41.0
48	35.17993415010782	37.0	34.0	40.0	27.0	41.0
49	35.154988741573185	37.0	34.0	40.0	27.0	41.0
50	35.033224749685864	37.0	34.0	40.0	27.0	41.0
51	34.03478013269103	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	17.0
9	44.0
10	40.0
11	65.0
12	61.0
13	51.0
14	83.0
15	88.0
16	147.0
17	330.0
18	616.0
19	1136.0
20	1861.0
21	2981.0
22	4550.0
23	6731.0
24	10070.0
25	14189.0
26	18958.0
27	23191.0
28	26777.0
29	31837.0
30	38989.0
31	49209.0
32	64575.0
33	93514.0
34	155352.0
35	157922.0
36	167624.0
37	250628.0
38	411962.0
39	524252.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.081080109270395	4.598365220061671	54.183304875477035	17.137249795190897
2	21.7524759306788	4.5089586368217125	52.136525796228796	21.602039636270696
3	21.76953120824251	4.459201929627301	51.74857896982237	22.022687892307825
4	19.797873095129574	4.898217407656119	50.53065722584977	24.77325227136453
5	18.713283777467012	5.942622353395069	50.41340826641607	24.930685602721848
6	22.023270978720262	6.669099432754101	52.80173021174783	18.50589937677781
7	84.89611829657136	2.417719218625335	8.577687032255369	4.108475452547942
8	87.28332265961262	2.6367653475632333	6.69164544070157	3.3882665521225803
9	82.16455281645314	4.7330581813340435	8.646539819457011	4.455849182755803
10	42.38406541452099	25.320041554624993	17.144927099621285	15.150965931232733
11	32.85463558556938	24.564555926247344	23.585942564044753	18.994865924138516
12	30.13026150453787	21.209087984824205	28.4981540455993	20.162496465038622
13	28.67249688291722	20.266820342330032	30.243914764428233	20.816768010324516
14	22.875063046218344	22.611265035126095	32.65269332472957	21.86097859392599
15	21.1588453722861	24.282633645835254	33.747583835678476	20.810937146200175
16	26.77829208158934	23.13234992570507	30.094401690173143	19.99495630253244
17	26.923237645613685	23.375837093430853	28.315016321560492	21.38590893939497
18	26.947581503332824	22.82997103032341	29.857231291915408	20.365216174428358
19	26.352736181580884	25.232821545431666	27.96987775593363	20.44456451705382
20	26.184369979990418	28.069536941925566	27.336111416151688	18.409981661932328
21	26.36430072876084	25.021063996649197	29.929679778660397	18.68495549592957
22	24.240457062002495	22.295232199586593	31.378503741957054	22.085806996453865
23	23.888515821563896	23.84784554429659	30.43958884633438	21.824049787805137
24	24.21232314260253	25.365084979985557	28.327844222634052	22.09474765477786
25	25.231558191538056	25.220042234892475	26.40341610892832	23.144983464641154
26	22.81840648314346	25.959055672118847	26.63786543726136	24.584672407476337
27	21.8566540363671	24.76134759044399	30.629869378925505	22.7521289942634
28	20.526779701207087	25.647250213069494	29.5755033736408	24.25046671208262
29	23.194594400232457	26.178684887469185	28.71292420751268	21.913796504785683
30	25.632575871689887	23.358636044264035	29.825987578315793	21.18280050573028
31	25.136029200967535	24.850219677805885	28.7960140212846	21.21773709994198
32	26.43529149947474	24.46640304682087	27.86205536016762	21.23625009353678
33	26.02081424130254	23.81665042123134	27.672066370782705	22.490468966683412
34	22.64697907788771	23.86917678888482	31.404888402119713	22.078955731107758
35	23.705523869128196	25.18102403579373	28.565937841044814	22.547514254033256
36	25.931650610734426	24.752066798379406	27.594467287394547	21.721815303491617
37	23.344884923037455	26.257255781544732	28.286153544144987	22.11170575127283
38	23.374476558468505	25.318583838593906	29.331238757365107	21.97570084557248
39	22.82452889047402	23.76368673876854	28.700630802317185	24.711153568440253
40	25.142103017763727	23.008687015734587	29.2865840562795	22.562625910222184
41	22.53662997433448	23.07700530705816	28.835080810917713	25.551283907689648
42	22.05951951735994	23.929526232571792	29.795181179525503	24.215773070542763
43	22.24455227223916	23.12661624264947	30.094304509104408	24.534526976006966
44	23.56840138113735	23.10922083134517	28.958695130353828	24.363682657163654
45	23.379627155111677	22.649457195140556	27.648645733216586	26.322269916531184
46	22.879630556449083	23.37287307083431	29.2527650443583	24.49473132835831
47	21.369776881984283	24.546042932652547	31.128311080488274	22.955869104874893
48	21.313120318909395	25.65196349490334	30.44653729274922	22.588378893438044
49	23.562230383272418	23.467624612854916	30.084149087421174	22.885995916451492
50	22.217001439251625	23.136577302195224	29.42151997022372	25.224901288329427
51	21.715935848832903	23.85333627468035	28.525073201640026	25.905654674846723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	410.0
1	1615.5
2	2821.0
3	7941.5
4	13062.0
5	8743.5
6	4425.0
7	4460.0
8	4495.0
9	4574.5
10	4654.0
11	4705.5
12	4757.0
13	4815.0
14	4873.0
15	4681.5
16	4490.0
17	4470.5
18	4451.0
19	4679.0
20	4907.0
21	5873.5
22	6840.0
23	7451.5
24	8063.0
25	9729.5
26	14664.5
27	17933.0
28	20922.5
29	23912.0
30	28405.0
31	32898.0
32	37460.0
33	42022.0
34	48949.0
35	55876.0
36	58414.0
37	60952.0
38	67406.0
39	73860.0
40	79850.5
41	85841.0
42	92103.5
43	98366.0
44	108928.0
45	119490.0
46	132113.5
47	144737.0
48	163550.0
49	182363.0
50	184928.0
51	187493.0
52	171914.0
53	156335.0
54	146787.5
55	137240.0
56	131493.5
57	125747.0
58	120779.5
59	115812.0
60	102760.0
61	89708.0
62	80219.0
63	70730.0
64	61900.0
65	53070.0
66	45225.0
67	37380.0
68	31105.0
69	24830.0
70	20982.0
71	17134.0
72	14117.0
73	11100.0
74	8815.5
75	5112.0
76	3693.0
77	2556.0
78	1419.0
79	1217.5
80	1016.0
81	796.5
82	577.0
83	387.0
84	197.0
85	122.0
86	47.0
87	39.5
88	32.0
89	21.5
90	11.0
91	10.0
92	9.0
93	8.0
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2058014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.105144866707573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5489088931233	18.464560125501375
2	10.57026887841712	5.307362629454248
3	3.9699359450336016	2.989974510348546
4	1.9168983681197997	1.9249604490561167
5	1.1529723875980176	1.4472769408980974
6	0.7669584802428038	1.1552762251947282
7	0.5339116580777556	0.938275066544627
8	0.40970658599873394	0.822859455547392
9	0.3416274598955632	0.7718946184011102
>10	5.081146808174884	33.41942333543351
>50	1.5987449756786603	26.18900587682208
>100	0.10168434557807889	4.278685623793356
>500	0.004888658149792737	0.8087447120597928
>1k	0.0021510095859088043	1.1078143239250506
>5k	1.9554632599170948E-4	0.37388610701999403
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7616	0.370065509758437	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3347	0.16263251853485933	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	3325	0.1615635267787294	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3122	0.15169964830171223	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2671	0.1297853173010485	RNA PCR Primer, Index 35 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2192	0.10651045133803755	RNA PCR Primer, Index 35 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2112	0.10262320858847414	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.373148093258841E-4	0.0	0.0	0.1722534443400288	0.0
2	4.373148093258841E-4	0.0	0.0	0.6372648582565522	0.0
3	4.373148093258841E-4	0.0	0.0	0.8956207294994106	0.0
4	4.373148093258841E-4	0.0	0.0	1.2065515589301141	0.0
5	4.373148093258841E-4	0.0	0.0	1.7650997515080071	0.0
6	4.373148093258841E-4	0.0	0.0	2.305718036903539	0.0
7	4.373148093258841E-4	0.0	0.0	2.744247609588662	0.0
8	4.373148093258841E-4	0.0	0.0	3.390550307237949	0.0
9	4.373148093258841E-4	0.0	0.0	3.77397821394801	0.0
10	4.373148093258841E-4	0.0	0.0	4.440300211757549	0.0
11	4.373148093258841E-4	0.0	0.0	5.619446709303241	0.0
12	4.373148093258841E-4	0.0	0.0	6.563755154240933	0.0
13	4.373148093258841E-4	0.0	0.0	6.898398164443973	0.0
14	4.8590534369542674E-4	0.0	0.0	7.060204643894551	0.0
15	4.8590534369542674E-4	0.0	0.0	7.24839578350779	0.0
16	4.8590534369542674E-4	0.0	0.0	7.640861529610586	0.0
17	4.8590534369542674E-4	0.0	0.0	8.186727592718029	0.0
18	4.8590534369542674E-4	0.0	0.0	8.769716824083801	0.0
19	4.8590534369542674E-4	0.0	0.0	9.172823897213528	0.0
20	5.344958780649694E-4	0.0	0.0	9.653238510525195	0.0
21	5.830864124345121E-4	0.0	0.0	10.283943646641859	0.0
22	5.830864124345121E-4	0.0	0.0	10.966543473465196	0.0
23	5.830864124345121E-4	0.0	0.0	11.592146603473058	0.0
24	5.830864124345121E-4	0.0	0.0	12.073824570678333	0.0
25	5.830864124345121E-4	0.0	0.0	12.52688271313995	0.0
26	6.316769468040548E-4	0.0	0.0	12.929989786269676	0.0
27	6.316769468040548E-4	0.0	0.0	13.41710989332434	0.0
28	6.316769468040548E-4	0.0	0.0	13.851169136847465	0.0
29	6.316769468040548E-4	0.0	0.0	14.370456177654768	0.0
30	6.316769468040548E-4	0.0	0.0	14.99066575834761	0.0
31	6.316769468040548E-4	0.0	0.0	15.461022131044784	0.0
32	6.316769468040548E-4	0.0	0.0	15.92156321579931	0.0
33	6.316769468040548E-4	0.0	0.0	16.384193693531724	0.0
34	6.802674811735974E-4	0.0	0.0	16.89701819326788	0.0
35	7.774485499126828E-4	0.0	0.0	17.468297105850592	0.0
36	8.260390842822255E-4	0.0	0.0	17.949440577177803	0.0
37	8.260390842822255E-4	0.0	0.0	18.456337031720874	0.0
38	8.260390842822255E-4	0.0	0.0	19.042727600492512	0.0
39	8.260390842822255E-4	0.0	0.0	20.122797998458708	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	20	7.034589E-4	45.0	43
CTTACGA	20	7.034589E-4	45.0	45
CGTTTTT	3780	0.0	40.714287	1
TTCGGGT	695	0.0	40.467625	4
TCCGATA	195	0.0	39.23077	14
TCGTTTG	745	0.0	38.65772	1
GGGCGAT	3375	0.0	38.6	7
TGGGCGA	2430	0.0	38.055553	6
CGTTTGG	1310	0.0	37.614502	2
TACGGGT	60	1.5643309E-10	37.500004	4
TATCGTG	30	1.1402355E-4	37.500004	1
CGTTCAT	390	0.0	37.5	17
TTTCGCG	675	0.0	37.333336	1
TTTGGGC	5275	0.0	37.27962	4
CGGGTAT	290	0.0	37.24138	6
TCGTTCA	400	0.0	37.125	16
TTTTGCG	1700	0.0	36.92647	1
TACCGGT	55	2.752131E-9	36.81818	40
TTTGGGA	8935	0.0	36.790714	4
TTGGGAC	3585	0.0	36.71548	5
>>END_MODULE