##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934367.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1253294
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14664396382653	33.0	31.0	34.0	30.0	34.0
2	32.32751054421389	34.0	31.0	34.0	30.0	34.0
3	32.396624415340696	34.0	31.0	34.0	30.0	34.0
4	35.9015378674118	37.0	35.0	37.0	35.0	37.0
5	35.864136427685764	37.0	35.0	37.0	35.0	37.0
6	35.45002768703912	37.0	35.0	37.0	33.0	37.0
7	35.805797362789576	37.0	35.0	37.0	35.0	37.0
8	35.89040879474409	37.0	35.0	37.0	35.0	37.0
9	37.64568169958525	39.0	38.0	39.0	35.0	39.0
10	37.16472112688643	39.0	37.0	39.0	34.0	39.0
11	37.09382555090825	39.0	37.0	39.0	33.0	39.0
12	36.92746474490423	39.0	37.0	39.0	33.0	39.0
13	36.86245047052009	39.0	37.0	39.0	33.0	39.0
14	38.02927006751808	40.0	37.0	41.0	33.0	41.0
15	38.06000826621687	40.0	37.0	41.0	33.0	41.0
16	38.11870877862656	40.0	37.0	41.0	33.0	41.0
17	38.05618952935225	40.0	37.0	41.0	33.0	41.0
18	38.00524856897105	40.0	37.0	41.0	33.0	41.0
19	38.03271778210061	40.0	37.0	41.0	33.0	41.0
20	37.968246077935426	40.0	37.0	41.0	33.0	41.0
21	37.81832754325801	40.0	36.0	41.0	33.0	41.0
22	37.86026423169663	40.0	36.0	41.0	33.0	41.0
23	37.804769670963076	40.0	36.0	41.0	33.0	41.0
24	37.76420137653256	40.0	36.0	41.0	33.0	41.0
25	37.677046247727986	40.0	36.0	41.0	33.0	41.0
26	37.5903076213562	40.0	36.0	41.0	33.0	41.0
27	37.507431616204975	39.0	36.0	41.0	33.0	41.0
28	37.3827114787113	39.0	36.0	41.0	32.0	41.0
29	37.29213017855348	39.0	35.0	41.0	32.0	41.0
30	37.267892449816244	39.0	36.0	41.0	32.0	41.0
31	37.22141572528074	39.0	35.0	41.0	32.0	41.0
32	37.08304436149858	39.0	35.0	41.0	31.0	41.0
33	37.03256777739301	39.0	35.0	41.0	31.0	41.0
34	36.927104095288094	39.0	35.0	41.0	31.0	41.0
35	36.86507395710823	39.0	35.0	41.0	31.0	41.0
36	36.70276327820926	39.0	35.0	41.0	30.0	41.0
37	36.67208492181403	39.0	35.0	41.0	30.0	41.0
38	36.59018953254384	39.0	35.0	41.0	30.0	41.0
39	36.509932226596476	39.0	35.0	41.0	30.0	41.0
40	36.368426721902445	39.0	35.0	41.0	30.0	41.0
41	36.28682416097101	39.0	35.0	40.0	30.0	41.0
42	36.21144679540475	39.0	35.0	40.0	30.0	41.0
43	36.145459086216	38.0	35.0	40.0	30.0	41.0
44	36.01068863331349	38.0	35.0	40.0	29.0	41.0
45	36.05425622399852	38.0	35.0	40.0	29.0	41.0
46	35.96429090061869	38.0	35.0	40.0	29.0	41.0
47	35.754302661626085	38.0	35.0	40.0	28.0	41.0
48	35.654746611728775	38.0	34.0	40.0	28.0	41.0
49	35.627006113489735	38.0	34.0	40.0	28.0	41.0
50	35.502039425705384	38.0	34.0	40.0	28.0	41.0
51	34.52688435434942	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	25.0
10	24.0
11	39.0
12	34.0
13	29.0
14	29.0
15	57.0
16	81.0
17	168.0
18	317.0
19	533.0
20	917.0
21	1561.0
22	2412.0
23	3557.0
24	5149.0
25	7457.0
26	10179.0
27	12960.0
28	15268.0
29	17833.0
30	22060.0
31	27877.0
32	35950.0
33	48967.0
34	80039.0
35	97690.0
36	107952.0
37	159669.0
38	264164.0
39	330187.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.57429142723096	4.850657547231536	52.32842413671492	16.246626888822576
2	21.917762312753432	4.991247065732382	51.15663204323966	21.934358578274534
3	22.33665843768501	4.820018287807969	49.76310426763393	23.080219006873087
4	19.716762387755786	5.167662176632139	49.62387117468048	25.49170426093159
5	18.023065617484804	6.602840195516774	48.46596249563151	26.90813169136691
6	22.417804601314618	7.568934344216122	52.1196143921538	17.893646662315465
7	83.44107607632367	3.0055198540805272	8.922647040518825	4.630757029076976
8	83.87433435411005	4.126725253611682	7.49113934958597	4.507801042692297
9	77.99438918561806	6.283202504759458	10.219788812521244	5.5026194971012385
10	37.75506784521429	29.55260298062545	17.6742248825894	15.018104291570852
11	28.51788965717541	26.586499257157538	25.428032049941997	19.46757903572506
12	27.63150545682019	22.254714376674585	30.19730406432968	19.916476102175547
13	25.713200573847793	21.846510076646023	30.636706151948385	21.803583197557796
14	20.74932138827761	25.174300682840578	30.453349333835476	23.623028595046332
15	18.660904783713956	26.81828844628635	33.60991116210562	20.91089560789408
16	22.165190290546352	23.818034714919246	32.721372638822174	21.295402355712227
17	21.814115442984647	23.94952820327872	29.77848772913618	24.457868624600454
18	21.76504475406409	25.270367527491555	30.128445520364732	22.83614219807962
19	24.383504588707837	26.002677743610036	29.114158369863734	20.49965929781839
20	27.093164093979542	25.381434842901985	28.095562573506292	19.429838489612177
21	26.019114429654973	25.416542327658153	28.27253621257263	20.291807030114242
22	23.198307819234753	24.944107288473415	27.729168096232808	24.128416796059025
23	23.247857246583802	25.477661267029124	28.731406996283393	22.543074490103677
24	24.14014588755711	24.35358343692701	28.37905551291237	23.127215162603505
25	23.445735797027673	25.42292550670473	26.89161521558389	24.239723480683704
26	22.234367993463625	26.516044918430953	27.40801440045193	23.841572687653496
27	21.698739481717777	26.38359395321449	28.676192497530508	23.241474067537226
28	19.99722331711474	26.782143694935108	30.190841095545018	23.029791892405136
29	21.209229438583446	26.664214462049607	28.68895885562366	23.437597243743287
30	22.202452098230744	24.789554565808185	28.619461993754058	24.388531342207013
31	22.504536046609974	27.054226701795432	26.341784130459413	24.099453121135184
32	22.334823273709123	27.5471677036673	26.06108383188621	24.05692519073737
33	22.845716966649483	26.615782091033708	26.420137653256138	24.118363289060667
34	21.87643122842685	25.7248498756078	27.50527809117414	24.893440804791215
35	21.79352969055944	24.556408951131978	26.5525886184726	27.097472739835982
36	20.565246462521962	27.42867994261522	25.882115449367827	26.123958145494992
37	20.957492814934085	28.357432493892095	27.160506632920928	23.524568058252893
38	21.400645020242656	27.644590973865668	26.740333872180035	24.21443013371164
39	22.36554232287077	25.43226090606035	27.097472739835982	25.104724031232895
40	23.121071352771178	24.521540835590052	28.069391539415335	24.287996272223435
41	20.53157519305127	25.718147537608893	27.731960737065688	26.01831653227415
42	20.117546242142705	27.003959166803636	27.092286406860637	25.786208184193015
43	20.90594864413298	25.858338107419325	27.833373494168168	25.402339754279524
44	21.210665653868922	25.185391456434004	27.158272520254624	26.445670369442446
45	21.751480498590116	23.992774241319275	26.686555588712622	27.569189671377988
46	20.928529140010248	25.40417491825541	27.296867295303418	26.370428646430927
47	19.966105319262677	25.834640555208914	28.81893633895957	25.380317786568835
48	20.13757346640134	24.238367055136305	29.788780605348787	25.83527887311357
49	20.812754230052967	24.460501685957166	29.32376601180569	25.40297807218418
50	20.002489439828164	24.699152792561044	28.105616080504653	27.192741687106135
51	19.398161963593537	24.25695806410946	27.395567201311106	28.949312770985898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	1231.0
2	2185.0
3	4169.0
4	6153.0
5	4430.5
6	2708.0
7	2711.0
8	2714.0
9	2724.0
10	2734.0
11	2744.0
12	2754.0
13	2662.5
14	2571.0
15	2617.0
16	2663.0
17	2654.0
18	2645.0
19	2907.0
20	3169.0
21	3354.0
22	3539.0
23	4818.5
24	6098.0
25	6791.5
26	9937.5
27	12390.0
28	14362.0
29	16334.0
30	18746.5
31	21159.0
32	25003.0
33	28847.0
34	31664.0
35	34481.0
36	38158.0
37	41835.0
38	44999.0
39	48163.0
40	52879.5
41	57596.0
42	60951.0
43	64306.0
44	68846.0
45	73386.0
46	78171.0
47	82956.0
48	92521.5
49	102087.0
50	110727.5
51	119368.0
52	114987.0
53	110606.0
54	97509.0
55	84412.0
56	77332.5
57	70253.0
58	65707.0
59	61161.0
60	58785.5
61	56410.0
62	49289.0
63	42168.0
64	34450.0
65	26732.0
66	22613.0
67	18494.0
68	15078.5
69	11663.0
70	9632.5
71	7602.0
72	6595.5
73	5589.0
74	4637.5
75	2721.0
76	1756.0
77	1362.0
78	968.0
79	820.5
80	673.0
81	481.0
82	289.0
83	176.5
84	64.0
85	106.0
86	148.0
87	79.0
88	10.0
89	7.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1253294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.109150440824052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27746153186835	18.87103116616424
2	11.12532945185972	5.809458007246721
3	4.487263053634819	3.5147587840470904
4	2.272446446369218	2.3732658454787963
5	1.362236207375937	1.7783415037157968
6	0.8735128677218007	1.368400732520648
7	0.6513088129608672	1.190358384672087
8	0.4645431140244789	0.97030608402512
9	0.37292406477213935	0.8763057459125457
>10	4.507953693607841	29.607577115509905
>50	1.4592507084592024	25.73985733104431
>100	0.13896125532535203	5.801339466930706
>500	0.004951848742013872	0.8272043920417912
>1k	0.001856943278255202	1.271795440690217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3214	0.25644421819620933	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	3157	0.25189620312552363	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3052	0.24351828062689201	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2926	0.2334647736285341	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1913	0.15263776895125963	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1477	0.11784944314741792	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.191589523288231E-4	0.0	0.0	0.2671360430992249	0.0
2	3.191589523288231E-4	0.0	0.0	0.9919460238379821	0.0
3	3.191589523288231E-4	0.0	0.0	1.3939267242961348	0.0
4	3.191589523288231E-4	0.0	0.0	1.9114429654973215	0.0
5	3.191589523288231E-4	0.0	0.0	2.754980076502401	0.0
6	3.191589523288231E-4	0.0	0.0	3.3453443485726413	0.0
7	3.191589523288231E-4	0.0	0.0	3.8355724993497136	0.0
8	3.191589523288231E-4	0.0	0.0	4.626847331910948	0.0
9	3.191589523288231E-4	0.0	0.0	5.040078385438692	0.0
10	3.191589523288231E-4	0.0	0.0	5.863029744018562	0.0
11	3.191589523288231E-4	0.0	0.0	7.277462431001824	0.0
12	3.191589523288231E-4	0.0	0.0	8.524735616702865	0.0
13	3.191589523288231E-4	0.0	0.0	8.932780337255265	0.0
14	3.191589523288231E-4	0.0	0.0	9.074646491565426	0.0
15	3.191589523288231E-4	0.0	0.0	9.321436151453689	0.0
16	3.9894869041102885E-4	0.0	0.0	9.88179948200502	0.0
17	3.9894869041102885E-4	0.0	0.0	10.649137393141594	0.0
18	3.9894869041102885E-4	0.0	0.0	11.472487700411875	0.0
19	4.7873842849323464E-4	0.0	0.0	12.037239466557727	0.0
20	4.7873842849323464E-4	0.0	0.0	12.66606239238359	0.0
21	5.585281665754404E-4	0.0	0.0	13.489811648344283	0.0
22	6.383179046576462E-4	0.0	0.0	14.393191062911017	0.0
23	6.383179046576462E-4	0.0	0.0	15.201540899421843	0.0
24	6.383179046576462E-4	0.0	0.0	15.825895599915103	0.0
25	6.383179046576462E-4	0.0	0.0	16.365593388303143	0.0
26	6.383179046576462E-4	0.0	0.0	16.878481824695562	0.0
27	6.383179046576462E-4	0.0	0.0	17.367513129401402	0.0
28	7.18107642739852E-4	0.0	0.0	17.892768975196564	0.0
29	7.18107642739852E-4	0.0	0.0	18.44228090136871	0.0
30	7.18107642739852E-4	0.0	0.0	19.029692953129913	0.0
31	7.18107642739852E-4	0.0	0.0	19.621493440485633	0.0
32	7.978973808220577E-4	0.0	0.0	20.2229484861493	0.0
33	7.978973808220577E-4	0.0	0.0	20.79344511343707	0.0
34	7.978973808220577E-4	0.0	0.0	21.3399250295621	0.0
35	7.978973808220577E-4	0.0	0.0	21.893346652900277	0.0
36	7.978973808220577E-4	0.0	0.0	22.45817820878421	0.0
37	7.978973808220577E-4	0.0	0.0	23.012796678193624	0.0
38	7.978973808220577E-4	0.0	0.0	23.575713280363587	0.0
39	7.978973808220577E-4	0.0	0.0	24.149241917698482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAGG	45	3.8562575E-10	45.000004	10
CATATCG	20	7.033532E-4	45.0	33
CTATACG	25	3.891009E-5	45.0	23
TCGATCC	25	3.891009E-5	45.0	20
TAGTTCG	20	7.033532E-4	45.0	1
CGCTAAT	20	7.033532E-4	45.0	22
TACTCGA	20	7.033532E-4	45.0	45
TAAGCGT	25	3.891009E-5	45.0	13
CCGTTGA	25	3.891009E-5	45.0	2
ATCGACT	25	3.891009E-5	45.0	30
AATCGAC	25	3.891009E-5	45.0	29
TATTGCG	80	0.0	42.1875	1
GCTACGA	185	0.0	41.351353	10
GTGCGAT	60	3.6379788E-12	41.249996	14
GGCGATA	110	0.0	40.909092	8
AGCGATC	45	1.9284926E-8	40.000004	14
TTTCGCG	395	0.0	39.873417	1
TGGGCGA	1470	0.0	39.642857	6
CGAAACA	40	3.4585537E-7	39.375	21
CGCACGA	40	3.4585537E-7	39.375	17
>>END_MODULE