##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934366.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030301
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27204574197249	33.0	31.0	34.0	30.0	34.0
2	32.4614428210785	34.0	31.0	34.0	31.0	34.0
3	32.52520574084661	34.0	31.0	34.0	31.0	34.0
4	35.99325925142264	37.0	35.0	37.0	35.0	37.0
5	35.94441430222818	37.0	35.0	37.0	35.0	37.0
6	35.545770604900895	37.0	35.0	37.0	33.0	37.0
7	35.87959052742839	37.0	35.0	37.0	35.0	37.0
8	35.96723675896656	37.0	35.0	37.0	35.0	37.0
9	37.721722098687664	39.0	38.0	39.0	35.0	39.0
10	37.31582421059477	39.0	37.0	39.0	34.0	39.0
11	37.272737772747966	39.0	37.0	39.0	34.0	39.0
12	37.045923472849196	39.0	37.0	39.0	33.0	39.0
13	36.93133171762427	39.0	37.0	39.0	33.0	39.0
14	38.11956797091335	40.0	37.0	41.0	33.0	41.0
15	38.19343667530168	40.0	37.0	41.0	33.0	41.0
16	38.23570684683408	40.0	37.0	41.0	34.0	41.0
17	38.167265682552966	40.0	37.0	41.0	33.0	41.0
18	38.143970548412554	40.0	37.0	41.0	33.0	41.0
19	38.17656199498981	40.0	37.0	41.0	34.0	41.0
20	38.11741520196525	40.0	37.0	41.0	34.0	41.0
21	37.96823937858937	40.0	36.0	41.0	33.0	41.0
22	38.02181692534512	40.0	37.0	41.0	34.0	41.0
23	37.97456956753415	40.0	36.0	41.0	34.0	41.0
24	37.928785859666256	40.0	36.0	41.0	33.0	41.0
25	37.85346321123633	40.0	36.0	41.0	33.0	41.0
26	37.78897331944742	40.0	36.0	41.0	33.0	41.0
27	37.703975828422955	40.0	36.0	41.0	33.0	41.0
28	37.587281774937615	40.0	36.0	41.0	33.0	41.0
29	37.49422256214446	39.0	36.0	41.0	33.0	41.0
30	37.46368682550051	39.0	36.0	41.0	33.0	41.0
31	37.42403336500693	39.0	36.0	41.0	33.0	41.0
32	37.297519850995	39.0	35.0	41.0	32.0	41.0
33	37.22042587554511	39.0	35.0	41.0	32.0	41.0
34	37.11924185262365	39.0	35.0	41.0	32.0	41.0
35	37.05273216273691	39.0	35.0	41.0	31.0	41.0
36	36.88559848044407	39.0	35.0	41.0	31.0	41.0
37	36.870877539670445	39.0	35.0	41.0	31.0	41.0
38	36.80188216841486	39.0	35.0	41.0	31.0	41.0
39	36.73473965375167	39.0	35.0	41.0	31.0	41.0
40	36.61024690842773	39.0	35.0	41.0	31.0	41.0
41	36.53426814105781	39.0	35.0	41.0	30.0	41.0
42	36.46785259841541	39.0	35.0	41.0	30.0	41.0
43	36.397496459772434	39.0	35.0	40.0	30.0	41.0
44	36.26656287822685	38.0	35.0	40.0	30.0	41.0
45	36.2637772845023	38.0	35.0	40.0	30.0	41.0
46	36.18566418939708	38.0	35.0	40.0	30.0	41.0
47	36.00896145883581	38.0	35.0	40.0	30.0	41.0
48	35.90526943097211	38.0	35.0	40.0	29.0	41.0
49	35.89314578943435	38.0	35.0	40.0	29.0	41.0
50	35.79794739595516	38.0	35.0	40.0	29.0	41.0
51	34.90273133773528	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	33.0
10	36.0
11	47.0
12	30.0
13	32.0
14	43.0
15	47.0
16	89.0
17	124.0
18	235.0
19	402.0
20	639.0
21	1044.0
22	1645.0
23	2432.0
24	3567.0
25	5107.0
26	7201.0
27	9066.0
28	10729.0
29	12823.0
30	16256.0
31	20763.0
32	27102.0
33	38314.0
34	65714.0
35	83260.0
36	85703.0
37	128896.0
38	217965.0
39	290806.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.59465631888157	4.611273792804239	52.4399180433679	17.354151844946283
2	21.290089012822467	4.809177123966686	50.90473560639075	22.995998256820094
3	22.075684678555103	4.614573799307193	50.0778898593712	23.231851662766513
4	19.59058566380116	5.108798302631949	48.53717505855085	26.76344097501604
5	17.946988307300487	6.529548161168436	48.51824855066626	27.005214980864817
6	22.69288295362229	7.42782934307547	51.66626063645479	18.213027066847456
7	84.15783348749541	2.68203175576846	8.5944787008845	4.5656560558516395
8	84.97536156909486	3.482768627808767	6.874398840727128	4.66747096236925
9	78.3549661700804	5.961073511527213	10.054828637456433	5.629131680935959
10	37.057228906892256	31.090137736447893	16.81411548663934	15.03851787002051
11	29.52311994261871	26.143136811475483	24.926890297107352	19.40685294879846
12	29.673658474562288	21.297659615976304	29.18186044660735	19.84682146285406
13	26.512931657835914	22.44839129535932	29.560196486269547	21.478480560535225
14	19.912336297839175	26.28416355996937	29.332204860521344	24.471295281670113
15	19.120334737130218	26.692684953232117	32.82972645857861	21.35725385105906
16	22.098008251957438	23.564667024490902	32.65065257628596	21.6866721472657
17	21.094320979985458	24.86904312429086	29.108969126497986	24.92766676922569
18	22.0475375642652	25.125084805314174	29.95842962396426	22.868948006456367
19	24.60106318444804	25.721318333186126	28.23388504912642	21.443733433239412
20	27.867390209268944	24.987940417412	26.997741436725775	20.146927936593286
21	26.571458243755952	25.45722075393502	27.42994522959795	20.54137577271108
22	23.36132838850006	24.5694219456256	27.610474997112494	24.458774668761848
23	23.2802841111481	26.005215951454964	27.348221539142443	23.366278398254494
24	24.146924054232695	23.20535455172809	29.005018921654933	23.642702472384283
25	22.935821667648582	25.119261264426605	27.034138567273057	24.91077850065175
26	21.73966636934255	27.670845704313596	27.394712807228178	23.194775119115675
27	22.06015523618826	27.8357489704465	28.428973668859875	21.675122124505364
28	20.247286957889006	27.359771561902786	29.786441049751485	22.60650043045673
29	20.981926640855438	26.76790568969651	29.154683922465374	23.09548374698268
30	21.997066876572962	26.243787009815577	28.374232384516755	23.3849137290947
31	22.768977221219817	27.66473098638165	25.529626778970417	24.036665013428117
32	23.2373840266097	27.60727204962433	26.195451620448786	22.959892303317186
33	23.44149913471888	26.630567183764743	26.947561926077913	22.98037175543846
34	21.291836075088735	25.940283470558605	27.94921095873924	24.818669495613417
35	21.98706979804931	24.500510045122738	28.651238812735308	24.861181344092646
36	21.38006271953536	28.027537583677002	26.98017375504828	23.612225941739357
37	21.147218143047517	28.896895179175797	27.61542500686692	22.340461670909765
38	21.079665068751755	28.982695348252598	26.313378323421986	23.624261259573657
39	21.844101869259568	26.306098897312534	27.594945554745653	24.25485367868225
40	22.723456543282012	24.96154036538837	29.242716448882415	23.072286642447207
41	20.11713081905191	24.933587369128052	29.59542890863932	25.353852903180723
42	21.439948131662494	26.1268308969903	27.145756434284742	25.28746453706247
43	21.693466278301194	25.877486287987683	28.246405662034686	24.182641771676433
44	21.41684808614182	26.33511954273557	27.6506574292367	24.597374941885917
45	21.878072524437034	26.22534579700495	26.58621121400445	25.31037046455356
46	21.241559505426082	26.69579084170548	27.30211850711588	24.76053114575255
47	20.104319029099262	26.72849972969064	29.44314331442947	23.72403792678062
48	20.57534642788855	24.51992184808129	30.19573891513257	24.708992808897595
49	20.574958191829378	25.319494011944084	29.541367037399752	24.56418075882679
50	20.352595988939154	25.301926330266593	28.755577253637526	25.589900427156724
51	19.620188663312955	25.11295242846508	27.068885694568866	28.1979732136531
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	976.0
2	1762.0
3	3272.0
4	4782.0
5	3450.5
6	2119.0
7	2108.0
8	2097.0
9	2172.0
10	2247.0
11	2267.5
12	2288.0
13	2172.5
14	2057.0
15	2007.0
16	1957.0
17	1896.5
18	1836.0
19	2167.5
20	2499.0
21	2553.0
22	2607.0
23	3468.5
24	4330.0
25	5314.5
26	7378.0
27	8457.0
28	10534.5
29	12612.0
30	15206.5
31	17801.0
32	19938.5
33	22076.0
34	26225.5
35	30375.0
36	32161.0
37	33947.0
38	36239.5
39	38532.0
40	44526.5
41	50521.0
42	56977.0
43	63433.0
44	71124.5
45	78816.0
46	87322.5
47	95829.0
48	93748.0
49	91667.0
50	83173.5
51	74680.0
52	69837.0
53	64994.0
54	58440.5
55	51887.0
56	51399.5
57	50912.0
58	50138.0
59	49364.0
60	48644.5
61	47925.0
62	43455.5
63	38986.0
64	32101.5
65	25217.0
66	21459.0
67	17701.0
68	14441.5
69	11182.0
70	8895.5
71	6609.0
72	5680.5
73	4752.0
74	3584.5
75	1940.0
76	1463.0
77	1058.0
78	653.0
79	427.5
80	202.0
81	157.5
82	113.0
83	82.5
84	52.0
85	38.0
86	24.0
87	22.5
88	21.0
89	14.5
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1030301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.116337161277155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96359234914964	17.354982564287972
2	11.348012446597586	5.47344988545034
3	4.500635928555559	3.256165604796106
4	2.3557903147820016	2.2725213405021596
5	1.2953053189345174	1.5619009899110228
6	0.8811179509173723	1.2749602549906203
7	0.6055392994315486	1.0222372936646353
8	0.4531456980297653	0.8742571549494484
9	0.35278853090595147	0.7657170442163614
>10	4.176887680036806	25.79729860009184
>50	1.8600183280795552	30.86120395489222
>100	0.2006540554181173	7.3896963423345845
>500	0.00407006197602672	0.7458797453098917
>1k	0.0024420371856160323	1.349729224602753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2823	0.27399759875997404	No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2679	0.2600211006298159	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2649	0.257109330186033	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2373	0.23032104210323	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2175	0.21110335717426268	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1052	0.10210608356198819	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.705901479276445E-5	0.0	0.0	0.27632701511500035	0.0
2	9.705901479276445E-5	0.0	0.0	1.1016198178978764	0.0
3	9.705901479276445E-5	0.0	0.0	1.58099429195934	0.0
4	9.705901479276445E-5	0.0	0.0	2.1055012078994393	0.0
5	9.705901479276445E-5	0.0	0.0	2.903326309495963	0.0
6	9.705901479276445E-5	0.0	0.0	3.450836211941947	0.0
7	9.705901479276445E-5	0.0	0.0	3.8910959030419265	0.0
8	9.705901479276445E-5	0.0	0.0	4.58778551122439	0.0
9	9.705901479276445E-5	0.0	0.0	4.962530367339253	0.0
10	9.705901479276445E-5	0.0	0.0	5.646117008524693	0.0
11	9.705901479276445E-5	0.0	0.0	6.897887122306976	0.0
12	9.705901479276445E-5	0.0	0.0	7.985918678133865	0.0
13	9.705901479276445E-5	0.0	0.0	8.342901734541654	0.0
14	9.705901479276445E-5	0.0	0.0	8.478104942147974	0.0
15	9.705901479276445E-5	0.0	0.0	8.715025997257111	0.0
16	9.705901479276445E-5	0.0	0.0	9.241182916448688	0.0
17	1.941180295855289E-4	0.0	0.0	9.998437349861836	9.705901479276445E-5
18	1.941180295855289E-4	0.0	0.0	10.824603683777848	9.705901479276445E-5
19	1.941180295855289E-4	0.0	0.0	11.32921350168543	9.705901479276445E-5
20	1.941180295855289E-4	0.0	0.0	11.91069405930888	9.705901479276445E-5
21	1.941180295855289E-4	0.0	0.0	12.705413272432036	9.705901479276445E-5
22	1.941180295855289E-4	0.0	0.0	13.516341341025583	9.705901479276445E-5
23	1.941180295855289E-4	0.0	0.0	14.261172220545259	9.705901479276445E-5
24	1.941180295855289E-4	0.0	0.0	14.806255647621423	9.705901479276445E-5
25	1.941180295855289E-4	0.0	0.0	15.319891953904733	9.705901479276445E-5
26	1.941180295855289E-4	0.0	0.0	15.781213451214741	9.705901479276445E-5
27	1.941180295855289E-4	0.0	0.0	16.274273246361986	9.705901479276445E-5
28	1.941180295855289E-4	0.0	0.0	16.756074195793268	9.705901479276445E-5
29	1.941180295855289E-4	0.0	0.0	17.287763478828033	9.705901479276445E-5
30	1.941180295855289E-4	0.0	0.0	17.858955780883452	9.705901479276445E-5
31	3.882360591710578E-4	0.0	0.0	18.421121594563143	9.705901479276445E-5
32	3.882360591710578E-4	0.0	0.0	18.951257933361223	9.705901479276445E-5
33	3.882360591710578E-4	0.0	0.0	19.44276478427178	9.705901479276445E-5
34	3.882360591710578E-4	0.0	0.0	19.936503992522574	9.705901479276445E-5
35	3.882360591710578E-4	0.0	0.0	20.47780211802182	9.705901479276445E-5
36	3.882360591710578E-4	0.0	0.0	21.01677082716604	9.705901479276445E-5
37	3.882360591710578E-4	0.0	0.0	21.555351300251093	9.705901479276445E-5
38	3.882360591710578E-4	0.0	0.0	22.060446413232636	9.705901479276445E-5
39	3.882360591710578E-4	0.0	0.0	22.595047466711183	9.705901479276445E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	25	3.890523E-5	45.0	25
CGTTGAC	40	6.8139343E-9	45.0	26
ATAGCGG	20	7.0329476E-4	45.0	2
CCCCGTA	20	7.0329476E-4	45.0	38
CTACGTT	20	7.0329476E-4	45.0	30
AATTGCG	25	3.890523E-5	45.0	33
TACGAGT	20	7.0329476E-4	45.0	30
TGCGACG	20	7.0329476E-4	45.0	1
TGTTACG	20	7.0329476E-4	45.0	21
GCGATAG	25	3.890523E-5	45.0	9
TGTACGC	20	7.0329476E-4	45.0	26
TTCGATC	25	3.890523E-5	45.0	13
AAAATCG	20	7.0329476E-4	45.0	18
GTGCGAT	20	7.0329476E-4	45.0	16
CTTACCG	20	7.0329476E-4	45.0	28
ATCTCGA	30	2.1652831E-6	44.999996	20
TAGCGGG	155	0.0	43.548386	3
CGTTGAT	65	0.0	41.538464	25
CGGGATC	255	0.0	40.588234	6
TCGTTAG	50	1.0804797E-9	40.5	1
>>END_MODULE