##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056422
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22103004291846	33.0	31.0	34.0	30.0	34.0
2	32.421920406807125	34.0	31.0	34.0	31.0	34.0
3	32.48099717726439	34.0	31.0	34.0	30.0	34.0
4	35.957465861180474	37.0	35.0	37.0	35.0	37.0
5	35.92794924755448	37.0	35.0	37.0	35.0	37.0
6	35.55708324892893	37.0	35.0	37.0	33.0	37.0
7	35.929239451658525	37.0	35.0	37.0	35.0	37.0
8	36.025533356935014	37.0	35.0	37.0	35.0	37.0
9	37.80825370921848	39.0	38.0	39.0	35.0	39.0
10	37.254870686146255	39.0	37.0	39.0	34.0	39.0
11	37.15279026752567	39.0	37.0	39.0	34.0	39.0
12	36.990743282513996	39.0	37.0	39.0	33.0	39.0
13	36.93426206572752	39.0	37.0	39.0	33.0	39.0
14	38.10874063584439	40.0	37.0	41.0	33.0	41.0
15	38.16683768418302	40.0	37.0	41.0	33.0	41.0
16	38.20066412853954	40.0	37.0	41.0	33.0	41.0
17	38.13003704958814	40.0	37.0	41.0	33.0	41.0
18	38.097054964777335	40.0	37.0	41.0	33.0	41.0
19	38.139081730596295	40.0	37.0	41.0	34.0	41.0
20	38.062333044938484	40.0	37.0	41.0	34.0	41.0
21	37.92984148380098	40.0	36.0	41.0	33.0	41.0
22	37.989008180443044	40.0	37.0	41.0	33.0	41.0
23	37.95146447158427	40.0	37.0	41.0	33.0	41.0
24	37.91842843106259	40.0	36.0	41.0	33.0	41.0
25	37.83133823415264	40.0	36.0	41.0	33.0	41.0
26	37.770648471917475	40.0	36.0	41.0	33.0	41.0
27	37.679603416059116	40.0	36.0	41.0	33.0	41.0
28	37.558583596327985	40.0	36.0	41.0	33.0	41.0
29	37.48165221852631	39.0	36.0	41.0	33.0	41.0
30	37.46966837116228	39.0	36.0	41.0	33.0	41.0
31	37.41147003754182	39.0	36.0	41.0	32.0	41.0
32	37.315751659848054	39.0	36.0	41.0	32.0	41.0
33	37.25711032144351	39.0	35.0	41.0	32.0	41.0
34	37.14598806158902	39.0	35.0	41.0	31.0	41.0
35	37.07872706172344	39.0	35.0	41.0	31.0	41.0
36	36.91170857857939	39.0	35.0	41.0	31.0	41.0
37	36.860898390983905	39.0	35.0	41.0	31.0	41.0
38	36.79975142509338	39.0	35.0	41.0	31.0	41.0
39	36.76877800727361	39.0	35.0	41.0	31.0	41.0
40	36.661992082709375	39.0	35.0	41.0	30.0	41.0
41	36.58013085679776	39.0	35.0	41.0	30.0	41.0
42	36.520519262188785	39.0	35.0	41.0	30.0	41.0
43	36.45624949120711	39.0	35.0	40.0	30.0	41.0
44	36.29094433853138	39.0	35.0	40.0	30.0	41.0
45	36.32826275863244	39.0	35.0	40.0	30.0	41.0
46	36.21431397680094	39.0	35.0	40.0	30.0	41.0
47	36.003296031320815	38.0	35.0	40.0	29.0	41.0
48	35.90574599923137	38.0	35.0	40.0	29.0	41.0
49	35.917244245197466	38.0	35.0	40.0	29.0	41.0
50	35.83864875968126	38.0	35.0	40.0	29.0	41.0
51	34.9248794515828	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	21.0
11	16.0
12	14.0
13	15.0
14	23.0
15	26.0
16	42.0
17	80.0
18	178.0
19	333.0
20	581.0
21	975.0
22	1498.0
23	2440.0
24	3694.0
25	5498.0
26	7829.0
27	9919.0
28	11589.0
29	14047.0
30	17501.0
31	21985.0
32	28990.0
33	39684.0
34	66553.0
35	80986.0
36	84284.0
37	127883.0
38	225834.0
39	303794.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.91273941663464	4.449358305677087	56.14546081016867	17.49244146751961
2	19.94231471892861	4.6934842326267345	54.80414076950309	20.56006027894156
3	20.87650579029971	4.433076933270985	52.65802870443819	22.032388571991117
4	18.2587072211673	4.4412176194740365	52.38881810488611	24.91125705447255
5	18.07999076126775	5.695356590453436	51.743526734581444	24.481125913697365
6	21.585692081384146	6.6149701539725605	53.02984981380546	18.769487950837828
7	85.522736179292	2.1355102411725615	8.162457805687499	4.179295773847951
8	87.93133804483435	2.453848935368631	6.006311871581622	3.6085011482153915
9	82.78150208912726	5.094649675981757	7.89107004587182	4.232778189019161
10	36.872670201870086	36.77924162881879	14.175301158059941	12.172787011251186
11	26.25503823282741	25.873183254419164	29.724958397307137	18.14682011544629
12	25.843270965580047	21.88055530839002	33.044938481023685	19.231235245006257
13	24.23491748562601	22.89946631175799	32.76380082959272	20.10181537302328
14	19.5104797135993	26.231278788211533	31.927392651800133	22.33084884638904
15	17.000687225370164	27.020262735914248	35.32925289325667	20.64979714545892
16	21.023511437664116	24.98130481947555	33.97941352982047	20.015770213039865
17	19.867250019405127	25.46491837542194	30.988941919043718	23.678889686129217
18	20.578992107320747	25.819606180106057	32.30281080855946	21.298590904013736
19	23.04429479885879	27.09494879887015	29.91172088426784	19.94903551800322
20	25.120075121495013	26.8928515309223	30.17051897821136	17.81655436937133
21	24.43692009443196	26.332374751756397	30.376213293551253	18.85449186026039
22	21.74415148491796	24.89516500035024	30.533820764808002	22.826862749923798
23	21.144296502723346	26.565330899962326	30.347815550982464	21.942557046331864
24	22.413675595547993	24.86203430068666	30.589669658526613	22.13462044523874
25	20.882942611948636	26.262043009327712	29.47714076382355	23.377873614900103
26	20.922131496693556	28.418851557426862	28.845290991668104	21.81372595421148
27	20.69996649066377	27.736643121782773	31.108780392683983	20.454609994869475
28	19.14263428819165	27.155814627109244	32.636484283742675	21.065066800956437
29	20.842996454068544	25.31658750007099	31.644267158389354	22.196148887471107
30	21.534481485618436	24.68189795365867	32.15429061492472	21.629329945798176
31	21.56855877670098	27.139533254703142	29.391095603840135	21.90081236475575
32	22.653636520254217	27.25672127237032	28.126922763819763	21.9627194435557
33	22.018095041564827	26.342503279939265	29.56612035720574	22.073281321290168
34	21.197305622185073	25.131812855090107	30.609358760040966	23.06152276268385
35	21.259875314978295	25.16096787079406	29.247876322151566	24.331280492076083
36	21.538930465287546	25.567623544379046	30.126218499804057	22.767227490529354
37	21.118738534411438	26.820058650804317	31.348835976532108	20.712366838252137
38	20.814220074932177	26.157539316674587	29.69760190529921	23.330638703094028
39	21.68290702011128	24.52145070814504	29.811571512141928	23.98407075960175
40	21.61143936797984	24.153510623595494	30.147232829304954	24.087817179119707
41	18.75727692153325	24.6812353396654	30.02171480715093	26.53977293165042
42	20.419964748935556	24.88503647216737	29.471271897025996	25.223726881871073
43	21.7688575209528	24.390821092328636	29.442306199605838	24.398015187112726
44	20.90831126197675	25.407649594574895	30.286287108750106	23.397752034698254
45	22.03484970968041	23.87189967645505	28.54825060439862	25.545000009465912
46	21.78939855474422	24.523249231841064	30.228166395625987	23.459185817788725
47	20.049658185838616	24.5527828841126	31.148915868847865	24.248643061200923
48	20.137217892092366	23.62890965920816	31.38329190418223	24.850580544517246
49	20.31839548968121	23.230583990110013	32.008136899837375	24.442883620371404
50	20.368564834886058	23.004727277546284	30.593361365060552	26.033346522507106
51	18.811327291555838	22.801494099895685	29.470798601316517	28.916380007231957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	909.0
2	1588.0
3	3200.5
4	4813.0
5	3402.0
6	1991.0
7	2000.0
8	2009.0
9	2050.5
10	2092.0
11	2196.5
12	2301.0
13	2146.5
14	1992.0
15	2077.5
16	2163.0
17	2329.0
18	2495.0
19	2808.0
20	3121.0
21	3706.0
22	4291.0
23	4922.5
24	5554.0
25	8258.0
26	12985.5
27	15009.0
28	16939.5
29	18870.0
30	21647.5
31	24425.0
32	27670.5
33	30916.0
34	34685.0
35	38454.0
36	42662.0
37	46870.0
38	48239.0
39	49608.0
40	54549.5
41	59491.0
42	61872.0
43	64253.0
44	67588.0
45	70923.0
46	73225.0
47	75527.0
48	79698.5
49	83870.0
50	90585.5
51	97301.0
52	91754.0
53	86207.0
54	73406.0
55	60605.0
56	53046.0
57	45487.0
58	42851.5
59	40216.0
60	37524.5
61	34833.0
62	30247.0
63	25661.0
64	21154.0
65	16647.0
66	13297.5
67	9948.0
68	8087.5
69	6227.0
70	5010.5
71	3794.0
72	3278.5
73	2763.0
74	2269.5
75	1239.5
76	703.0
77	466.5
78	230.0
79	181.0
80	132.0
81	80.5
82	29.0
83	24.0
84	19.0
85	18.0
86	17.0
87	9.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1056422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.93105917265115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69147551079999	17.624133589617742
2	11.995938760833603	5.981429181556841
3	4.992767077794389	3.734249143552694
4	2.507822894114121	2.5009072387075366
5	1.4347396496917357	1.7884789551906723
6	0.9162862683128244	1.3706392304636839
7	0.6463532406183748	1.127998962180407
8	0.4507011821750073	0.8989166273591146
9	0.3496534931924544	0.7845508735834731
>10	4.083406562087029	26.36055729112349
>50	1.771068364734977	29.94039846922681
>100	0.15403926198915807	6.035195685141931
>500	0.003448640193787107	0.5157739850270076
>1k	0.002299093462524738	1.336770767268553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	3482	0.32960313208168707	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2763	0.26154320905850126	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2482	0.23494399018574016	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2175	0.20588363362368448	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2064	0.19537646887323434	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2874798139379907	0.0
2	0.0	0.0	0.0	1.1189657163519882	0.0
3	0.0	0.0	0.0	1.5467303785797721	0.0
4	0.0	0.0	0.0	2.0515475823108567	0.0
5	0.0	0.0	0.0	2.902817245381107	0.0
6	0.0	0.0	0.0	3.400724331753788	0.0
7	0.0	0.0	0.0	3.8865150479637873	0.0
8	0.0	0.0	0.0	4.627980106434739	0.0
9	9.465914189594689E-5	0.0	0.0	5.0243179335530685	0.0
10	9.465914189594689E-5	0.0	0.0	5.837913258148732	0.0
11	9.465914189594689E-5	0.0	0.0	7.363818625511396	0.0
12	9.465914189594689E-5	0.0	0.0	8.81428065678299	0.0
13	9.465914189594689E-5	0.0	0.0	9.24450645670007	0.0
14	9.465914189594689E-5	0.0	0.0	9.39955813112563	0.0
15	9.465914189594689E-5	0.0	0.0	9.64595587748078	0.0
16	9.465914189594689E-5	0.0	0.0	10.192801740213664	0.0
17	9.465914189594689E-5	0.0	0.0	11.069345394170133	0.0
18	9.465914189594689E-5	0.0	0.0	12.039412280319796	0.0
19	9.465914189594689E-5	0.0	0.0	12.6955894519425	0.0
20	9.465914189594689E-5	0.0	0.0	13.356594239801897	0.0
21	9.465914189594689E-5	0.0	0.0	14.215625952507615	0.0
22	9.465914189594689E-5	0.0	0.0	15.190993750603452	0.0
23	9.465914189594689E-5	0.0	0.0	16.067632063701815	0.0
24	9.465914189594689E-5	0.0	0.0	16.735546968919618	0.0
25	9.465914189594689E-5	0.0	0.0	17.290438858713657	0.0
26	9.465914189594689E-5	0.0	0.0	17.808981638019656	0.0
27	9.465914189594689E-5	0.0	0.0	18.318815776271226	0.0
28	9.465914189594689E-5	0.0	0.0	18.867270844416343	0.0
29	9.465914189594689E-5	0.0	0.0	19.481230038753452	0.0
30	9.465914189594689E-5	0.0	0.0	20.109009467807372	0.0
31	9.465914189594689E-5	0.0	0.0	20.721548775016046	0.0
32	9.465914189594689E-5	0.0	0.0	21.29575112975686	0.0
33	9.465914189594689E-5	0.0	0.0	21.85405074865915	0.0
34	9.465914189594689E-5	0.0	0.0	22.41793525693331	0.0
35	9.465914189594689E-5	0.0	0.0	22.99592397734996	0.0
36	9.465914189594689E-5	0.0	0.0	23.592276571294427	0.0
37	9.465914189594689E-5	0.0	0.0	24.172347792832788	0.0
38	9.465914189594689E-5	0.0	0.0	24.73869343879624	0.0
39	9.465914189594689E-5	0.0	0.0	25.338453761848957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCTA	45	3.8562575E-10	45.000004	24
TCGCCGA	20	7.0330274E-4	45.0	34
GTCGCGA	20	7.0330274E-4	45.0	16
GATCGAC	35	1.2118107E-7	45.0	9
CGGTTCA	25	3.8905906E-5	45.0	41
CGCATCG	35	1.2118107E-7	45.0	21
CGTTACA	25	3.8905906E-5	45.0	10
ATCCGAT	25	3.8905906E-5	45.0	43
GAACGAT	20	7.0330274E-4	45.0	14
CTCGACG	25	3.8905906E-5	45.0	1
CGTCGCG	20	7.0330274E-4	45.0	33
CCGAATA	20	7.0330274E-4	45.0	25
TTACCCG	25	3.8905906E-5	45.0	1
GCTCGAC	20	7.0330274E-4	45.0	21
CGATCCA	20	7.0330274E-4	45.0	35
TTCGATG	25	3.8905906E-5	45.0	1
GCGAGAC	30	2.165334E-6	44.999996	21
GGACGTA	30	2.165334E-6	44.999996	8
TTATGCG	100	0.0	42.75	1
TATGCGG	140	0.0	41.785713	2
>>END_MODULE