##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902698
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18386104765935	33.0	31.0	34.0	30.0	34.0
2	32.372599695579254	34.0	31.0	34.0	30.0	34.0
3	32.4370708697704	34.0	31.0	34.0	30.0	34.0
4	35.92221540315809	37.0	35.0	37.0	35.0	37.0
5	35.88700650715965	37.0	35.0	37.0	35.0	37.0
6	35.50114434727894	37.0	35.0	37.0	33.0	37.0
7	35.851088625431764	37.0	35.0	37.0	35.0	37.0
8	35.929589962534536	37.0	35.0	37.0	35.0	37.0
9	37.69647545469249	39.0	38.0	39.0	35.0	39.0
10	37.229128678694316	39.0	37.0	39.0	34.0	39.0
11	37.16219045572274	39.0	37.0	39.0	34.0	39.0
12	36.950070787793926	39.0	37.0	39.0	33.0	39.0
13	36.81308034359221	39.0	35.0	39.0	33.0	39.0
14	37.97742323567793	40.0	37.0	41.0	33.0	41.0
15	38.01150883241128	40.0	37.0	41.0	33.0	41.0
16	38.05453318828667	40.0	37.0	41.0	33.0	41.0
17	38.003781995750515	40.0	37.0	41.0	33.0	41.0
18	37.97448869943214	40.0	37.0	41.0	33.0	41.0
19	38.019306567644996	40.0	37.0	41.0	34.0	41.0
20	37.97864845164163	40.0	36.0	41.0	33.0	41.0
21	37.83054908729165	40.0	36.0	41.0	33.0	41.0
22	37.87636396668653	40.0	36.0	41.0	33.0	41.0
23	37.81983454045539	40.0	36.0	41.0	33.0	41.0
24	37.789402435809095	40.0	36.0	41.0	33.0	41.0
25	37.71302251694365	40.0	36.0	41.0	33.0	41.0
26	37.61309984069977	40.0	36.0	41.0	33.0	41.0
27	37.52249257226669	39.0	35.0	41.0	33.0	41.0
28	37.41463479480402	39.0	35.0	41.0	32.0	41.0
29	37.329370398516446	39.0	35.0	41.0	32.0	41.0
30	37.296737114738264	39.0	35.0	41.0	32.0	41.0
31	37.24467429860263	39.0	35.0	41.0	32.0	41.0
32	37.13954390061792	39.0	35.0	41.0	32.0	41.0
33	37.08314740921105	39.0	35.0	41.0	32.0	41.0
34	36.96018269676016	39.0	35.0	41.0	31.0	41.0
35	36.9085973382017	39.0	35.0	41.0	31.0	41.0
36	36.74269135414059	39.0	35.0	41.0	31.0	41.0
37	36.71844293440331	39.0	35.0	41.0	31.0	41.0
38	36.69277986657775	39.0	35.0	41.0	31.0	41.0
39	36.65231339827938	39.0	35.0	41.0	31.0	41.0
40	36.525128005157875	39.0	35.0	41.0	30.0	41.0
41	36.43914465302903	39.0	35.0	41.0	30.0	41.0
42	36.38145537045612	39.0	35.0	41.0	30.0	41.0
43	36.32911339118952	39.0	35.0	40.0	30.0	41.0
44	36.170684990993664	38.0	35.0	40.0	30.0	41.0
45	36.18069276768089	38.0	35.0	40.0	30.0	41.0
46	36.07555793853537	38.0	35.0	40.0	30.0	41.0
47	35.89020802084418	38.0	35.0	40.0	29.0	41.0
48	35.808662476265596	38.0	35.0	40.0	29.0	41.0
49	35.80813073696851	38.0	35.0	40.0	29.0	41.0
50	35.71825571785913	38.0	35.0	40.0	29.0	41.0
51	34.80679695756499	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	18.0
10	34.0
11	26.0
12	19.0
13	28.0
14	30.0
15	43.0
16	66.0
17	118.0
18	187.0
19	387.0
20	651.0
21	1048.0
22	1639.0
23	2441.0
24	3514.0
25	5034.0
26	7015.0
27	8541.0
28	10223.0
29	12420.0
30	15224.0
31	19357.0
32	25334.0
33	34857.0
34	60055.0
35	75436.0
36	73581.0
37	109188.0
38	186948.0
39	249130.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.305138595632204	4.67443153745771	54.120204099266864	16.90022576764322
2	21.840526953643412	5.157760402703895	51.10302670439062	21.898685939262077
3	21.68720878965058	4.858103152992474	50.41597522094876	23.03871283640819
4	19.654746105563543	5.490097463381995	49.77401079873889	25.08114563231557
5	18.306343871372263	6.413440596965985	48.57061830202349	26.70959722963826
6	21.286299515452566	7.052857101710649	52.89598514674897	18.764858236087818
7	82.67338578350677	2.79008040341288	9.603654821435297	4.932878991645047
8	83.51364465192124	3.8651907947065354	7.671557929673047	4.949606623699177
9	77.8599265756654	5.955590906371787	10.365925259610634	5.818557258352184
10	38.10831529481621	29.554070131982122	17.4231027431101	14.91451183009157
11	29.718798535058237	25.901353498069124	25.36263512270992	19.01721284416272
12	29.957638102665562	21.21772730193265	29.95952134600941	18.865113249392376
13	25.563588265399943	22.953191432793695	30.348688044063465	21.1345322577429
14	21.024639469678675	25.822478835668182	29.867131643140894	23.285750051512245
15	17.988740420384225	26.277559050756732	35.606814239092145	20.126886289766897
16	20.798650268417564	23.275558381651447	34.484955101263104	21.440836248667882
17	21.07969664273101	23.595045075983332	30.340268838526285	24.98498944275937
18	21.094208694380622	25.208098389494605	30.71580971709254	22.981883199032236
19	23.175414147367114	27.31987885206348	28.700407002120308	20.804299998449093
20	27.37969952298554	25.552178026316664	28.380255633667073	18.687866817030724
21	25.865128758455207	25.93558421531897	27.573673587401327	20.6256134388245
22	23.04901528528921	25.41082399650825	27.628619981433438	23.91154073676911
23	23.080698085073855	26.12003128399531	27.907893891423267	22.891376739507567
24	24.081697311836294	23.803863529109403	29.13244518100184	22.98199397805246
25	23.091332871015556	25.402294011950843	26.22150486652236	25.284868250511245
26	21.283862377007594	28.300827076165007	26.40728128344142	24.008029263385982
27	20.996944714622163	28.220623065521362	28.778727769420115	22.00370445043636
28	20.309450115099402	26.66982756137712	31.203680522167993	21.81704180135549
29	20.997277051682843	26.250861306882257	30.31611901211701	22.43574262931789
30	22.253511141046065	26.095216783464682	29.44949473688875	22.201777338600508
31	22.52447662451894	28.321431973927048	27.402741559192556	21.751349842361453
32	24.34191723034725	27.206108798291346	26.754795069890484	21.697178901470924
33	23.40516983531591	27.22383344152751	26.89005625358647	22.48094046957011
34	21.549510467509624	25.608786105652165	29.61699261547051	23.2247108113677
35	22.49467706807814	24.93635745288014	27.99751411878613	24.57145136025559
36	21.93291665651192	28.53390613472058	27.39864273544419	22.134534473323303
37	21.060199535171233	28.92185426355215	29.48350389609814	20.534442305178477
38	21.424551732694656	27.579544875473307	28.217964369035936	22.777939022796108
39	21.141400556996913	25.51218680001507	28.982893503696694	24.363519139291324
40	22.872765864109592	24.683338170683882	30.672052004103257	21.771843961103272
41	20.660730388236153	24.880524826686223	29.79025100310403	24.668493781973595
42	21.911425526588072	25.22560147469032	26.789912019302136	26.07306097941947
43	21.5902771469528	25.09898105457196	28.32896494730242	24.981776851172818
44	21.238553757735147	25.185610248388716	29.159475261936997	24.416360731939143
45	22.22038821399848	25.012794976836105	27.636596070889713	25.130220738275703
46	21.540537366871312	26.680683905359263	28.383357446233404	23.395421281536017
47	19.769291612477264	26.65298915030276	30.504775683561945	23.072943553658035
48	20.368384553859652	23.6000301318935	31.764000806471266	24.26758450777558
49	20.630376936694223	23.85781291195948	31.00937412069153	24.50243603065477
50	19.896798264757425	23.557934104207607	30.542662108479245	26.002605522555715
51	19.203653935203135	23.971361407691166	28.9297195739882	27.895265083117497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	910.5
2	1468.0
3	2862.5
4	4257.0
5	3050.0
6	1843.0
7	1780.0
8	1717.0
9	1755.5
10	1794.0
11	1877.0
12	1960.0
13	1991.0
14	2022.0
15	2058.0
16	2094.0
17	2156.0
18	2218.0
19	1934.5
20	1651.0
21	2144.5
22	2638.0
23	3270.5
24	3903.0
25	5070.0
26	7808.5
27	9380.0
28	10668.5
29	11957.0
30	13975.0
31	15993.0
32	18469.5
33	20946.0
34	24636.5
35	28327.0
36	29393.5
37	30460.0
38	33185.0
39	35910.0
40	42324.5
41	48739.0
42	52938.5
43	57138.0
44	65395.5
45	73653.0
46	81612.5
47	89572.0
48	86422.5
49	83273.0
50	73161.0
51	63049.0
52	57898.5
53	52748.0
54	47614.0
55	42480.0
56	42149.0
57	41818.0
58	42977.5
59	44137.0
60	41064.0
61	37991.0
62	33024.0
63	28057.0
64	23807.5
65	19558.0
66	15604.0
67	11650.0
68	9730.5
69	7811.0
70	6742.0
71	5673.0
72	4900.0
73	4127.0
74	3119.0
75	1521.5
76	932.0
77	745.5
78	559.0
79	420.5
80	282.0
81	212.0
82	142.0
83	101.0
84	60.0
85	32.5
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	902698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.554495104328723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28462752952262	18.216426652170824
2	11.820543846667615	6.041360597203412
3	4.716359618265723	3.615725663256754
4	2.452710505701685	2.507111144411574
5	1.3932642061468332	1.7802081667507845
6	0.9466441750051027	1.451460836142552
7	0.642339155415822	1.149025696167459
8	0.48587649649184317	0.9933062840727359
9	0.36347139651675275	0.8359495220565795
>10	4.157925757001673	26.380321660922007
>50	1.5248426517797156	26.930338635130706
>100	0.20482960988572546	7.798685344395189
>500	0.003501360852747444	0.6058610387590169
>1k	0.003063690746154013	1.694218758560382
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3268	0.3620258380986775	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2860	0.3168279978464558	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	2633	0.29168116025514623	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2136	0.23662398720280756	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1996	0.2211149243711629	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1254	0.13891689136344604	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1001	0.11088979924625954	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	938	0.10391072097201944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2155804045206703E-4	0.0	0.0	0.31416930136103105	0.0
2	2.2155804045206703E-4	0.0	0.0	1.3086325659301339	0.0
3	2.2155804045206703E-4	0.0	0.0	1.8960937101887896	0.0
4	2.2155804045206703E-4	0.0	0.0	2.538833585540236	0.0
5	2.2155804045206703E-4	0.0	0.0	3.5191171355204065	0.0
6	2.2155804045206703E-4	0.0	0.0	4.239513104050302	0.0
7	2.2155804045206703E-4	0.0	0.0	4.843591101342863	0.0
8	2.2155804045206703E-4	0.0	0.0	5.7797846012730725	0.0
9	2.2155804045206703E-4	0.0	0.0	6.266990732227168	0.0
10	2.2155804045206703E-4	0.0	0.0	7.0826566581514525	0.0
11	2.2155804045206703E-4	0.0	0.0	8.673775725657972	0.0
12	2.2155804045206703E-4	0.0	0.0	9.985177767093758	0.0
13	2.2155804045206703E-4	0.0	0.0	10.434719031171001	0.0
14	2.2155804045206703E-4	0.0	0.0	10.596567179721236	0.0
15	2.2155804045206703E-4	0.0	0.0	10.839173234016249	0.0
16	2.2155804045206703E-4	0.0	0.0	11.459757305322489	0.0
17	2.2155804045206703E-4	0.0	0.0	12.30400421846509	0.0
18	2.2155804045206703E-4	0.0	0.0	13.278748817433959	0.0
19	2.2155804045206703E-4	0.0	0.0	13.88448850002991	0.0
20	2.2155804045206703E-4	0.0	0.0	14.510833080387904	0.0
21	2.2155804045206703E-4	0.0	0.0	15.385544224092664	0.0
22	2.2155804045206703E-4	0.0	0.0	16.290054924238227	0.0
23	3.3233706067810055E-4	0.0	0.0	17.127765875187492	0.0
24	3.3233706067810055E-4	0.0	0.0	17.770062634458036	0.0
25	3.3233706067810055E-4	0.0	0.0	18.36151182344483	0.0
26	3.3233706067810055E-4	0.0	0.0	18.84772094321689	0.0
27	3.3233706067810055E-4	0.0	0.0	19.374142847331	0.0
28	3.3233706067810055E-4	0.0	0.0	19.882618550168495	0.0
29	3.3233706067810055E-4	0.0	0.0	20.4728491699328	0.0
30	3.3233706067810055E-4	0.0	0.0	21.076484051144458	0.0
31	5.538951011301676E-4	0.0	0.0	21.690642939277588	0.0
32	5.538951011301676E-4	0.0	0.0	22.296936516974668	0.0
33	5.538951011301676E-4	0.0	0.0	22.832220742706863	0.0
34	5.538951011301676E-4	0.0	0.0	23.378250533400983	0.0
35	5.538951011301676E-4	0.0	0.0	24.003265765516264	0.0
36	5.538951011301676E-4	0.0	0.0	24.582750820318644	0.0
37	5.538951011301676E-4	0.0	0.0	25.16323288630306	0.0
38	5.538951011301676E-4	0.0	0.0	25.73994846559979	0.0
39	5.538951011301676E-4	0.0	0.0	26.316553265876294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	25	3.8901373E-5	45.000004	1
TGCGATC	25	3.8901373E-5	45.000004	24
CGATAAC	65	0.0	45.000004	16
TCGAACG	25	3.8901373E-5	45.000004	38
AACCGAT	20	7.032481E-4	45.0	31
CTCACGA	20	7.032481E-4	45.0	24
CGAACCG	20	7.032481E-4	45.0	37
ACCGCAC	35	1.2115379E-7	45.0	25
CGGTTGC	20	7.032481E-4	45.0	19
TTCCGCA	20	7.032481E-4	45.0	44
TATGCGA	20	7.032481E-4	45.0	11
GCGTTAT	20	7.032481E-4	45.0	37
CGTTCGA	20	7.032481E-4	45.0	44
CGTTAGC	20	7.032481E-4	45.0	42
CCCGTCG	20	7.032481E-4	45.0	31
TACGAGT	20	7.032481E-4	45.0	30
GAACGTA	35	1.2115379E-7	45.0	9
ACTCGAC	20	7.032481E-4	45.0	32
ATGCGAT	20	7.032481E-4	45.0	12
CAGTCGT	20	7.032481E-4	45.0	19
>>END_MODULE