SRR2934363.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011855
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	3472	0.3431321681466218	TruSeq Adapter, Index 23 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	3317	0.32781376778293336	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2840	0.2806726260185501	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2827	0.27938785695578916	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2608	0.25774443966773897	Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1087	0.10742645932470563	TruSeq Adapter, Index 23 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	20	7.0328865E-4	45.0	29
ACGTTAT	25	3.8904735E-5	45.0	35
GACGTAT	40	6.8139343E-9	45.0	38
CGTTGCA	20	7.0328865E-4	45.0	22
TAGCGAT	20	7.0328865E-4	45.0	41
CGATTCA	20	7.0328865E-4	45.0	10
ATCCGTC	25	3.8904735E-5	45.0	26
CGGATTG	20	7.0328865E-4	45.0	6
TCATGCG	40	6.8139343E-9	45.0	1
TCCGTTG	35	1.211738E-7	45.0	1
CCGACGT	40	6.8139343E-9	45.0	36
ACGTCCG	20	7.0328865E-4	45.0	25
CGGTCAA	20	7.0328865E-4	45.0	25
ACTCCCG	20	7.0328865E-4	45.0	26
GACGATA	25	3.8904735E-5	45.0	42
TAGTCCG	20	7.0328865E-4	45.0	1
TACGTTC	25	3.8904735E-5	45.0	31
AATCGTA	25	3.8904735E-5	45.0	12
AGTCGTA	20	7.0328865E-4	45.0	44
CGCATCG	30	2.1652431E-6	44.999996	21