##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011855
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21352960651477	33.0	31.0	34.0	30.0	34.0
2	32.41130695603619	34.0	31.0	34.0	30.0	34.0
3	32.47229099030988	34.0	31.0	34.0	30.0	34.0
4	35.9525070291692	37.0	35.0	37.0	35.0	37.0
5	35.922897055408136	37.0	35.0	37.0	35.0	37.0
6	35.560842215534834	37.0	35.0	37.0	33.0	37.0
7	35.886611223940186	37.0	35.0	37.0	35.0	37.0
8	35.97423741543996	37.0	35.0	37.0	35.0	37.0
9	37.73852577691468	39.0	38.0	39.0	35.0	39.0
10	37.29294414713571	39.0	37.0	39.0	34.0	39.0
11	37.196943237914525	39.0	37.0	39.0	34.0	39.0
12	36.99683353840224	39.0	37.0	39.0	33.0	39.0
13	36.89944705516107	39.0	37.0	39.0	33.0	39.0
14	38.038796072559805	40.0	37.0	41.0	33.0	41.0
15	38.10805599616546	40.0	37.0	41.0	33.0	41.0
16	38.16933750389137	40.0	37.0	41.0	33.0	41.0
17	38.11393332048564	40.0	37.0	41.0	33.0	41.0
18	38.07438911701775	40.0	37.0	41.0	33.0	41.0
19	38.08430061619501	40.0	37.0	41.0	34.0	41.0
20	38.03568297829234	40.0	37.0	41.0	34.0	41.0
21	37.91396889870584	40.0	36.0	41.0	33.0	41.0
22	37.979667047155964	40.0	36.0	41.0	33.0	41.0
23	37.93244190126055	40.0	36.0	41.0	33.0	41.0
24	37.88589175326504	40.0	36.0	41.0	33.0	41.0
25	37.78256766038612	40.0	36.0	41.0	33.0	41.0
26	37.69249744281542	40.0	36.0	41.0	33.0	41.0
27	37.61697575245465	40.0	36.0	41.0	33.0	41.0
28	37.49767308557056	39.0	36.0	41.0	33.0	41.0
29	37.40916929797253	39.0	35.0	41.0	33.0	41.0
30	37.39403471841321	39.0	36.0	41.0	32.0	41.0
31	37.335954262221364	39.0	35.0	41.0	32.0	41.0
32	37.21799170829813	39.0	35.0	41.0	32.0	41.0
33	37.178828982413485	39.0	35.0	41.0	32.0	41.0
34	37.045212999886346	39.0	35.0	41.0	31.0	41.0
35	36.9485845303922	39.0	35.0	41.0	31.0	41.0
36	36.81801048569212	39.0	35.0	41.0	31.0	41.0
37	36.80082422876796	39.0	35.0	41.0	31.0	41.0
38	36.736825928616256	39.0	35.0	41.0	31.0	41.0
39	36.70117655197632	39.0	35.0	41.0	31.0	41.0
40	36.58080851505404	39.0	35.0	41.0	30.0	41.0
41	36.500852394858946	39.0	35.0	40.0	30.0	41.0
42	36.46234490119632	39.0	35.0	40.0	30.0	41.0
43	36.38675798409851	39.0	35.0	40.0	30.0	41.0
44	36.22463692920428	38.0	35.0	40.0	30.0	41.0
45	36.224128951282545	38.0	35.0	40.0	30.0	41.0
46	36.12431623108054	38.0	35.0	40.0	30.0	41.0
47	35.94937911064332	38.0	35.0	40.0	30.0	41.0
48	35.86821234267756	38.0	35.0	40.0	29.0	41.0
49	35.8732802624882	38.0	35.0	40.0	29.0	41.0
50	35.77975302785478	38.0	35.0	40.0	29.0	41.0
51	34.87750715270469	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	27.0
11	32.0
12	23.0
13	22.0
14	29.0
15	47.0
16	79.0
17	121.0
18	231.0
19	396.0
20	707.0
21	1005.0
22	1603.0
23	2456.0
24	3610.0
25	5170.0
26	7242.0
27	9081.0
28	10578.0
29	12928.0
30	16315.0
31	20815.0
32	27645.0
33	39066.0
34	66918.0
35	82503.0
36	84133.0
37	126558.0
38	215391.0
39	276993.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.375033972258873	4.916218232849568	53.41644800885502	17.292299786036537
2	21.133067484965732	4.5174456814464525	52.95215223525109	21.397334598336716
3	21.917863725533795	4.666083579168952	51.221172994154294	22.19487970114295
4	20.38572720399662	5.386542538209526	48.353864931240146	25.87386532655371
5	17.58838964080822	6.112931200616689	49.60107920601272	26.697599952562374
6	21.990601420163955	6.679613185683719	52.58935321760529	18.740432176547035
7	83.26687124143281	2.6257714791150906	9.39442904368709	4.7129282357650055
8	84.43087201229426	3.253727065636875	7.712073370196322	4.603327551872551
9	78.37387768010238	5.874260640111478	9.941147694086604	5.810713985699532
10	38.44938256963695	29.449278799828036	17.399528588582356	14.701810041952651
11	28.864610047882355	26.13487110307307	25.913297853941526	19.087220995103053
12	29.193510927949163	20.673416645665636	29.998962301910847	20.134110124474354
13	25.2108256617796	22.51617079522264	30.76043504257033	21.51256850042743
14	20.837175286972936	26.433629324359714	29.611752672072576	23.11744271659477
15	17.68939225481912	26.086741677414256	34.76318247179685	21.46068359596978
16	21.08533337286469	23.70764585834927	33.88924302395106	21.31777774483498
17	20.99035929060982	23.76822766107792	29.77887147862095	25.46254156969131
18	22.333140617973918	24.55440749909819	30.17250495377302	22.93994692915487
19	23.462057310583038	26.574262122537316	29.286706099194053	20.67697446768559
20	26.6452209061575	25.32517010836533	28.481551210400703	19.54805777507647
21	26.00540591290254	25.1611149818897	28.712513156529347	20.120965948678418
22	22.841612681658933	24.78576475878461	27.83037095235977	24.542251607196683
23	22.90170034244037	26.145544569132927	27.998972184749793	22.95378290367691
24	23.75745536662862	24.071630816668396	28.532546659353365	23.63836715734962
25	23.145905292754396	25.318845091440966	27.03183756565911	24.503412050145528
26	21.478176220901215	28.812231001477485	26.734067628266896	22.975525149354404
27	20.809602166318296	28.418696354714857	28.33983129993922	22.431870179027626
28	19.727035988358015	27.675506866102356	30.29722638125028	22.30023076428935
29	20.57864022018965	26.42710665065647	29.56194316379323	23.43230996536065
30	21.83395842289656	26.093956149843606	29.146172129405894	22.925913297853942
31	22.61322027365581	27.317945753097035	27.451858220792502	22.61697575245465
32	22.865726808683064	28.140296781653497	27.22494823863103	21.76902817103241
33	23.44772719411378	26.244570615354966	26.766779825172577	23.540922365358675
34	21.475310197607364	25.88434113583468	28.909181651521216	23.73116701503674
35	20.449866828745225	25.737778634290486	28.585518676094896	25.226835860869397
36	20.9445029179082	28.248019726146534	27.087082635357834	23.720394720587436
37	21.48519303655168	28.72298896581032	28.710338931961594	21.081479065676405
38	21.184655904255056	26.435012921811918	28.842274831868203	23.538056342064824
39	21.35315830825563	23.375681298209724	29.762762451141718	25.50839794239293
40	21.47451957049182	23.845412633233025	31.329093595426222	23.35097420084894
41	19.18595055615676	25.054973291627753	31.177095532462655	24.58198061975283
42	20.862870668228155	25.93296470344071	27.433772625524412	25.770392002806723
43	21.61712893645829	25.612068922918795	27.62549970104412	25.145302439578792
44	22.161772190679493	24.950906997544113	27.94145406209388	24.945866749682512
45	21.276368649658302	24.53651956060898	27.953609953995382	26.233501835737332
46	21.253638120086375	25.695183598440487	28.896926931230265	24.15425135024287
47	19.96392763785325	26.125482406075967	30.36561562674494	23.544974329325843
48	19.746999323025534	24.058387812483016	31.237578506801867	24.95703435768959
49	20.514500595441046	23.369553938064247	31.106235577231917	25.009709889262787
50	19.665268244956046	22.648996150634233	31.554422323356608	26.131313281053114
51	18.39344570121213	23.196900741707065	29.45026708372247	28.95938647335834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	1108.0
2	1960.0
3	3478.0
4	4996.0
5	3474.5
6	1953.0
7	1947.0
8	1941.0
9	2005.0
10	2069.0
11	2060.0
12	2051.0
13	1877.5
14	1704.0
15	1815.5
16	1927.0
17	1912.0
18	1897.0
19	2322.5
20	2748.0
21	3115.0
22	3482.0
23	4052.0
24	4622.0
25	6068.0
26	8416.0
27	9318.0
28	11603.5
29	13889.0
30	15866.5
31	17844.0
32	19745.0
33	21646.0
34	24902.0
35	28158.0
36	30983.5
37	33809.0
38	37759.5
39	41710.0
40	45896.5
41	50083.0
42	57970.0
43	65857.0
44	72558.5
45	79260.0
46	88666.0
47	98072.0
48	97627.0
49	97182.0
50	86212.0
51	75242.0
52	68376.5
53	61511.0
54	55681.5
55	49852.0
56	49078.0
57	48304.0
58	47573.0
59	46842.0
60	43743.5
61	40645.0
62	36743.0
63	32841.0
64	27479.0
65	22117.0
66	17934.5
67	13752.0
68	11462.0
69	9172.0
70	7473.0
71	5774.0
72	4870.5
73	3967.0
74	3396.0
75	2118.5
76	1412.0
77	1132.5
78	853.0
79	598.5
80	344.0
81	309.0
82	274.0
83	175.5
84	77.0
85	76.0
86	75.0
87	47.5
88	20.0
89	11.5
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1011855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.34896193516551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66320041643057	17.9123877757307
2	11.903761141292406	6.03495976111847
3	4.921995141446322	3.743024024567771
4	2.5209844472216973	2.5561735516706823
5	1.5643267598331394	1.9827029744585525
6	1.0214604323610366	1.5535776970918567
7	0.6800193000020057	1.2066448345650116
8	0.5024110690022844	1.0188479251155773
9	0.38837883755928865	0.8860500332742813
>10	4.037528655448122	25.396108223794418
>50	1.5974358546980254	27.89468028221187
>100	0.1906210421374587	7.335923685252103
>500	0.005513831797364503	0.8664736209077702
>1k	0.0023630707702990725	1.612445610240972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	3472	0.3431321681466218	TruSeq Adapter, Index 23 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	3317	0.32781376778293336	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2840	0.2806726260185501	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2827	0.27938785695578916	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2608	0.25774443966773897	Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1087	0.10742645932470563	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.964851683294543E-4	0.0	0.0	0.3716935726956926	0.0
2	2.964851683294543E-4	0.0	0.0	1.3361598252714075	0.0
3	2.964851683294543E-4	0.0	0.0	1.8799136239876266	0.0
4	2.964851683294543E-4	0.0	0.0	2.498776998680641	0.0
5	2.964851683294543E-4	0.0	0.0	3.538253998843708	0.0
6	2.964851683294543E-4	0.0	0.0	4.215129638139852	0.0
7	2.964851683294543E-4	0.0	0.0	4.784381161332404	0.0
8	2.964851683294543E-4	0.0	0.0	5.684510132380628	0.0
9	2.964851683294543E-4	0.0	0.0	6.121331613719357	0.0
10	2.964851683294543E-4	0.0	0.0	6.966413171847745	0.0
11	2.964851683294543E-4	0.0	0.0	8.548556858443156	0.0
12	2.964851683294543E-4	0.0	0.0	9.911499177253658	0.0
13	2.964851683294543E-4	0.0	0.0	10.378660974151435	0.0
14	3.953135577726058E-4	0.0	0.0	10.541134846395975	0.0
15	3.953135577726058E-4	0.0	0.0	10.812418775417427	0.0
16	4.941419472157572E-4	0.0	0.0	11.443833355569721	0.0
17	5.929703366589086E-4	0.0	0.0	12.29929189458964	0.0
18	6.917987261020601E-4	0.0	0.0	13.333827475280549	0.0
19	7.906271155452115E-4	0.0	0.0	13.980758112575419	0.0
20	7.906271155452115E-4	0.0	0.0	14.657040781534903	0.0
21	7.906271155452115E-4	0.0	0.0	15.582766305448903	0.0
22	7.906271155452115E-4	0.0	0.0	16.54663958768796	0.0
23	7.906271155452115E-4	0.0	0.0	17.427892336352542	0.0
24	8.894555049883629E-4	0.0	0.0	18.085002297760056	0.0
25	8.894555049883629E-4	0.0	0.0	18.68597773396386	0.0
26	8.894555049883629E-4	0.0	0.0	19.208384600560358	0.0
27	8.894555049883629E-4	0.0	0.0	19.74442978490001	0.0
28	8.894555049883629E-4	0.0	0.0	20.30636800727377	0.0
29	8.894555049883629E-4	0.0	0.0	20.905465704078154	0.0
30	8.894555049883629E-4	0.0	0.0	21.526107989781146	0.0
31	8.894555049883629E-4	0.0	0.0	22.157917883491212	0.0
32	8.894555049883629E-4	0.0	0.0	22.778955482751975	0.0
33	8.894555049883629E-4	0.0	0.0	23.34672458010288	0.0
34	8.894555049883629E-4	0.0	0.0	23.919138611757614	0.0
35	8.894555049883629E-4	0.0	0.0	24.53899027034506	0.0
36	8.894555049883629E-4	0.0	0.0	25.14895908998819	0.0
37	8.894555049883629E-4	0.0	0.0	25.748946242297563	0.0
38	8.894555049883629E-4	0.0	0.0	26.341521265398697	0.0
39	8.894555049883629E-4	0.0	0.0	26.986771820073034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	20	7.0328865E-4	45.0	29
ACGTTAT	25	3.8904735E-5	45.0	35
GACGTAT	40	6.8139343E-9	45.0	38
CGTTGCA	20	7.0328865E-4	45.0	22
TAGCGAT	20	7.0328865E-4	45.0	41
CGATTCA	20	7.0328865E-4	45.0	10
ATCCGTC	25	3.8904735E-5	45.0	26
CGGATTG	20	7.0328865E-4	45.0	6
TCATGCG	40	6.8139343E-9	45.0	1
TCCGTTG	35	1.211738E-7	45.0	1
CCGACGT	40	6.8139343E-9	45.0	36
ACGTCCG	20	7.0328865E-4	45.0	25
CGGTCAA	20	7.0328865E-4	45.0	25
ACTCCCG	20	7.0328865E-4	45.0	26
GACGATA	25	3.8904735E-5	45.0	42
TAGTCCG	20	7.0328865E-4	45.0	1
TACGTTC	25	3.8904735E-5	45.0	31
AATCGTA	25	3.8904735E-5	45.0	12
AGTCGTA	20	7.0328865E-4	45.0	44
CGCATCG	30	2.1652431E-6	44.999996	21
>>END_MODULE