FastQCFastQC Report
Sat 14 Jan 2017
SRR2934362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773818
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC38820.21884996093173031No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC38680.21806070295825164No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.21535467562061048No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT34990.19725811780013508No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC27480.15492006507995748No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.034324E-445.04
CGCGAAA207.034324E-445.019
TAGTCGT302.1661726E-644.99999622
CATACGA2900.041.12069318
ACATACG2900.041.12069317
GCGCGAC4150.040.662659
TCGTTTG5550.040.135141
TTTTGCG15600.040.0961571
ATTCCGA1150.039.13043212
TACGAAA3050.039.09836220
TTGGGAT101300.038.9807475
CGTTTTT23500.038.585111
ACTCGTT356.2502077E-638.5714332
ACGAAAA3100.038.4677421
TTTGGGA139450.038.3524554
TTCGTTG6250.038.161
TTGGGAC50750.038.0394065
TTGGGTA8150.037.8220865
TTTGTCG4650.037.7419361
TCTGGGA67900.037.7098664
Produced by FastQC (version 0.11.5)