FastQCFastQC Report
Sat 14 Jan 2017
SRR2934361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485186
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG48310.9957006179073592No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC45390.9355175128713524No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC40480.8343192095402587RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT35580.7333270127332611RNA PCR Primer, Index 16 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC32030.6601591966792117RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC30410.6267699397756736Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC28570.5888463393420256Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.27247282485479796No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT11770.24258737886089046No Hit
TCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG10680.22012176773443587RNA PCR Primer, Index 16 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT10090.20796148281277696No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC8550.17622107810200624No Hit
GTTCTTGGGCAATTTGTTTTGTGGGAATTGAATCCAGGGCCTCATGAATGT7910.16303026055986777No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT7470.15396157349964756No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA6800.14015243638522135No Hit
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG6180.12737383189127469Illumina PCR Primer Index 7 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG5800.11954178397562996Illumina PCR Primer Index 7 (95% over 21bp)
TTCGTGGGGTCAATTGTGTTTGCTTTGTAGTTTTTTAACTTTTAATTTCAT5710.11768682525876674No Hit
TCTTGCGGGCGCCAAAGAAAAGCAGATTTGAATTCTTTGATAATTGGGACA5210.10738149905397106No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG5020.10346547509614869RNA PCR Primer, Index 16 (95% over 24bp)
TTCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT4930.10161051637928548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC351.2099918E-745.06
GCCCCAC207.0292526E-445.09
CGGGTAG253.887462E-545.06
TCGTTCA700.045.016
GTCGCAC207.0292526E-445.019
TCGCACG207.0292526E-445.020
GTTCTAC351.2099918E-745.08
CGAAATT207.0292526E-445.036
CGCGGTA253.887462E-545.031
TACCACG253.887462E-545.023
TCCGATC207.0292526E-445.013
AACCCGA207.0292526E-445.024
GCGAAAT207.0292526E-445.035
AACCCAT253.887462E-545.019
ACACCGC253.887462E-545.033
GGCCGTA207.0292526E-445.045
CGGCACT207.0292526E-445.06
GACCGAA207.0292526E-445.011
CAACCCG207.0292526E-445.023
CGCGCAT253.887462E-545.036
Produced by FastQC (version 0.11.5)