FastQCFastQC Report
Sat 14 Jan 2017
SRR2934357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244361
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246681.0991101698879993No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT30450.13567336092544827Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG27400.12208374677692224No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC23260.10363751642449677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG207.034723E-445.00000426
CGTTTTT116150.043.5665131
ATACGTC403.4601908E-739.37500443
TCTTGCG5700.038.2894741
TTGGGAC43100.038.265665
TTTCGCG5350.037.8504681
ACACGCG6550.037.7862636
TTTGGGA120050.037.559354
GCGATCT4700.037.3404279
TTCTTCG6600.037.1590921
TGGGCGA22700.036.9713676
GGGCGAT32300.036.9195067
CGAGATA7450.036.8456419
TTGGGAT71350.036.580245
TTTGGGC47400.036.5031624
CGGGTAT2750.036.06
TATCTCG504.8825314E-836.01
TTGGGAG56700.035.952385
TTCGTTG9850.035.8629461
CACGCGG7050.035.7446837
Produced by FastQC (version 0.11.5)