##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2244361
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21968524671387	33.0	31.0	34.0	30.0	34.0
2	32.42937388414787	34.0	31.0	34.0	31.0	34.0
3	32.45682668697237	34.0	31.0	34.0	30.0	34.0
4	35.9653246514264	37.0	35.0	37.0	35.0	37.0
5	35.929676642928655	37.0	35.0	37.0	35.0	37.0
6	35.571793040424424	37.0	35.0	37.0	33.0	37.0
7	35.88571713730545	37.0	35.0	37.0	35.0	37.0
8	35.977871652555	37.0	35.0	37.0	35.0	37.0
9	37.75659708932743	39.0	38.0	39.0	35.0	39.0
10	37.31919018375386	39.0	37.0	39.0	34.0	39.0
11	37.20718859399179	39.0	37.0	39.0	34.0	39.0
12	36.95164191500387	39.0	37.0	39.0	33.0	39.0
13	36.84158920957903	39.0	37.0	39.0	33.0	39.0
14	37.804751998453014	40.0	37.0	41.0	33.0	41.0
15	37.90117097917848	40.0	37.0	41.0	33.0	41.0
16	38.01191474989986	40.0	37.0	41.0	33.0	41.0
17	37.9782053778336	40.0	37.0	41.0	33.0	41.0
18	37.89484757576878	40.0	37.0	41.0	33.0	41.0
19	37.86417381161052	40.0	37.0	41.0	33.0	41.0
20	37.712084642354775	40.0	35.0	41.0	33.0	41.0
21	37.56409240759397	39.0	35.0	41.0	33.0	41.0
22	37.67922851983259	39.0	35.0	41.0	33.0	41.0
23	37.65508356276018	39.0	35.0	41.0	33.0	41.0
24	37.595784278910564	39.0	35.0	41.0	33.0	41.0
25	37.51095968963995	39.0	35.0	41.0	33.0	41.0
26	37.449739591803635	39.0	35.0	41.0	33.0	41.0
27	37.35687886217948	39.0	35.0	41.0	33.0	41.0
28	37.21538558190951	39.0	35.0	41.0	32.0	41.0
29	37.104407891600324	39.0	35.0	41.0	32.0	41.0
30	37.015225714579785	39.0	35.0	41.0	32.0	41.0
31	36.87060860530013	39.0	35.0	41.0	31.0	41.0
32	36.673445582061	39.0	35.0	41.0	31.0	41.0
33	36.493551171135124	39.0	35.0	41.0	30.0	41.0
34	36.216523545009025	39.0	35.0	41.0	30.0	41.0
35	36.095789402863446	39.0	35.0	41.0	29.0	41.0
36	35.9174442079505	39.0	35.0	41.0	29.0	41.0
37	35.83436354490209	39.0	35.0	41.0	29.0	41.0
38	35.753643019104324	39.0	35.0	41.0	28.0	41.0
39	35.67467310294556	38.0	35.0	40.0	27.0	41.0
40	35.52402888840075	38.0	35.0	40.0	26.0	41.0
41	35.3795253972066	38.0	34.0	40.0	26.0	41.0
42	35.311272117097026	38.0	34.0	40.0	26.0	41.0
43	35.2353863750083	38.0	34.0	40.0	26.0	41.0
44	35.09078842485678	38.0	34.0	40.0	25.0	41.0
45	35.15438648238853	38.0	34.0	40.0	26.0	41.0
46	35.102373459528124	38.0	34.0	40.0	26.0	41.0
47	34.91974508557224	38.0	34.0	40.0	25.0	41.0
48	34.78730159720295	37.0	34.0	40.0	24.0	41.0
49	34.77136654932072	37.0	34.0	40.0	24.0	41.0
50	34.64478976421351	37.0	34.0	40.0	24.0	41.0
51	33.70104185556602	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	19.0
9	37.0
10	64.0
11	64.0
12	51.0
13	48.0
14	68.0
15	108.0
16	227.0
17	404.0
18	726.0
19	1361.0
20	2410.0
21	3844.0
22	6086.0
23	8939.0
24	13537.0
25	21344.0
26	29740.0
27	34699.0
28	36345.0
29	38553.0
30	44510.0
31	54134.0
32	68880.0
33	96164.0
34	156199.0
35	165821.0
36	179501.0
37	263149.0
38	437717.0
39	579423.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.51769612820754	4.4904986319045825	52.19222754271706	19.799577697170818
2	23.938751386252033	4.248113382829233	50.83464736733529	20.978487863583442
3	20.44626510619281	4.515984727947064	53.49642949596789	21.541320669892233
4	18.621781433557256	4.918326418967359	52.30709319935608	24.152798948119308
5	17.581752668131376	5.9712764568623315	52.18870760987203	24.258263265134264
6	21.641527365695627	6.301392690391608	54.314167818813466	17.742912125099306
7	81.57074552623219	2.4287091069573923	11.85843097433969	4.14211439247073
8	83.7251226518372	2.679916466201293	10.156432053488722	3.438528828472781
9	78.34390278569268	4.9498275901247615	12.100771667303077	4.6054979568794865
10	39.210225093022025	28.224113678681817	19.229838693507862	13.335822534788297
11	29.044703592692976	23.419138008546753	28.85845904469023	18.677699354070047
12	27.193441696768033	20.579309656512475	32.4814056205753	19.74584302614419
13	26.231653463948092	19.790488250330498	33.849189145596455	20.128669140124963
14	20.425546514130303	22.116941080334225	36.277363579210295	21.180148826325176
15	19.142152265165898	23.742481713057746	37.10918163343598	20.00618438834038
16	25.303193202876006	22.96364087595534	32.724860216337746	19.008305704830907
17	25.105542290210884	23.424484741982237	31.10190383810804	20.36806912969883
18	24.58142874519741	22.847705872629227	32.7076170010083	19.863248381165064
19	24.518470958994566	25.027435425940837	30.76421306554516	19.68988054951944
20	24.116352048534083	27.704856749872235	29.95895045404906	18.219840747544623
21	24.928075296264726	24.94384815989941	32.11185722795932	18.016219315876544
22	23.272592956302486	22.150447276529935	33.811405562652354	20.765554204515226
23	22.100499875020105	22.879474380458404	33.24291413012434	21.77711161439715
24	22.370554469624093	24.491559067369288	32.02172021345942	21.1161662495472
25	23.895398289312634	25.022578809736935	29.302059695387683	21.77996320556274
26	21.634576612229495	25.39787494079607	29.621527018157952	23.34602142881649
27	20.853463413417003	24.275907485471365	32.84872620759317	22.021902893518465
28	20.152907665032497	25.39270643180843	31.30539160144023	23.148994301718844
29	22.258094843031046	25.351046467123606	30.471746746624095	21.919111943221257
30	23.905646195063984	23.513062292563454	31.86145187873074	20.719839633641826
31	23.390532984666905	24.566547003801972	30.887856276240765	21.155063735290355
32	25.149296392157943	24.400174481734442	28.939105607342135	21.511423518765476
33	24.650089713731436	23.985891752708234	29.49623523131974	21.867783302240593
34	21.701990009628574	24.27354601153736	31.911131943568794	22.11333203526527
35	22.440953126524654	26.019209922111457	29.447357176497007	22.092479774866877
36	24.250020384421223	26.23120790282847	28.382020539476493	21.13675117327382
37	22.595117273914493	27.60353615126978	28.121679177280306	21.679667397535425
38	23.437762463347028	25.765774757269437	28.567552189687845	22.228910589695687
39	22.564596337220262	24.118624410244163	28.75780678776721	24.55897246476837
40	24.369029759472742	23.26595409562009	29.96661410530659	22.398402039600583
41	22.574086789068247	24.290432777971102	28.54941785211916	24.586062580841496
42	22.579478078615693	24.602904791163276	29.710104568739165	23.107512561481865
43	22.09176687707548	23.86719427044045	30.549898167006113	23.49114068547796
44	22.945016421155064	24.018818719448433	29.579867053473123	23.45629780592338
45	22.03638362990624	23.723545365473736	28.427200437006345	25.81287056761368
46	21.378156187885995	24.34452389789343	29.87843755973304	24.398882354487537
47	20.770143484047352	24.9754829993927	30.981290443025877	23.27308307353407
48	21.098744809769908	25.46216941035778	30.3116120802313	23.12747369964101
49	22.686546415661297	23.869422076038568	30.046503214055136	23.397528294245
50	20.951575971958164	24.210900118118253	30.01411092065849	24.823412989265094
51	20.862240967473593	24.236965443616246	29.632977938932285	25.267815649977877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	523.0
1	1728.5
2	2934.0
3	20853.5
4	38773.0
5	25073.5
6	11374.0
7	11110.5
8	10847.0
9	10915.5
10	10984.0
11	10653.0
12	10322.0
13	10009.0
14	9696.0
15	9256.5
16	8817.0
17	8550.0
18	8283.0
19	8137.5
20	7992.0
21	8688.5
22	9385.0
23	10691.5
24	11998.0
25	14615.5
26	20208.5
27	23184.0
28	27398.5
29	31613.0
30	35698.0
31	39783.0
32	45377.0
33	50971.0
34	57157.5
35	63344.0
36	68240.0
37	73136.0
38	78349.5
39	83563.0
40	91286.5
41	99010.0
42	102789.5
43	106569.0
44	113085.5
45	119602.0
46	129648.5
47	139695.0
48	156068.5
49	172442.0
50	182445.0
51	192448.0
52	189847.0
53	187246.0
54	171969.0
55	156692.0
56	143036.0
57	129380.0
58	119024.0
59	108668.0
60	98728.5
61	88789.0
62	80880.5
63	72972.0
64	62555.0
65	52138.0
66	43507.5
67	34877.0
68	29328.0
69	23779.0
70	19209.0
71	14639.0
72	11843.0
73	9047.0
74	7275.0
75	4313.0
76	3123.0
77	2269.0
78	1415.0
79	1187.0
80	959.0
81	644.5
82	330.0
83	236.0
84	142.0
85	110.5
86	79.0
87	49.0
88	19.0
89	23.5
90	28.0
91	18.5
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2244361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.15067421163553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4161405270956	20.47599061750096
2	9.099018874204729	4.940889941981106
3	3.288790176135338	2.67878611868034
4	1.695790594061792	1.8416743180211033
5	0.99986500477948	1.3573504500191533
6	0.7298829120356097	1.1890087894391204
7	0.530521717039611	1.0082815611077978
8	0.4579126234708337	0.994610916580155
9	0.38355818274514575	0.9372476934828375
>10	6.555882232929687	45.317358463647835
>50	0.7595243024768717	12.61518128539911
>100	0.07631138140330163	3.601879586634137
>500	0.004313128345668778	0.8253153741603844
>1k	0.0023224537245908807	1.1053749133728528
>5k	0.0	0.0
>10k+	1.6588955175649147E-4	1.1110499699731697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24668	1.0991101698879993	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	3045	0.13567336092544827	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2740	0.12208374677692224	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2326	0.10363751642449677	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.118927837366627E-4	0.0	0.0	0.1489956384022	0.0
2	3.118927837366627E-4	0.0	0.0	0.4722502306892697	0.0
3	3.118927837366627E-4	0.0	0.0	0.7256853955312893	0.0
4	3.118927837366627E-4	0.0	0.0	1.0471577433398638	0.0
5	3.118927837366627E-4	0.0	0.0	1.709350679324761	0.0
6	3.118927837366627E-4	0.0	0.0	2.517420325874492	0.0
7	3.118927837366627E-4	0.0	0.0	3.153280599689622	0.0
8	3.118927837366627E-4	0.0	0.0	3.98189061385401	0.0
9	3.118927837366627E-4	0.0	0.0	4.608884221388627	0.0
10	3.118927837366627E-4	0.0	0.0	5.398997754817518	0.0
11	3.118927837366627E-4	0.0	0.0	6.4872807894986595	0.0
12	3.118927837366627E-4	0.0	0.0	7.324311908823937	0.0
13	3.118927837366627E-4	0.0	0.0	7.641061308764499	0.0
14	3.118927837366627E-4	0.0	0.0	7.844281735424916	0.0
15	3.118927837366627E-4	0.0	0.0	8.01377318532981	0.0
16	3.564488956990431E-4	0.0	0.0	8.312477359925609	0.0
17	3.564488956990431E-4	0.0	0.0	8.718249871567007	0.0
18	3.564488956990431E-4	0.0	0.0	9.184395914917431	0.0
19	4.0100500766142343E-4	0.0	0.0	9.52431449307843	0.0
20	4.0100500766142343E-4	0.0	0.0	9.923849149045095	0.0
21	4.4556111962380384E-4	0.0	0.0	10.454467886405084	0.0
22	4.901172315861842E-4	0.0	0.0	11.028439720704467	0.0
23	4.901172315861842E-4	0.0	0.0	11.532681239782727	0.0
24	4.901172315861842E-4	0.0	0.0	11.942241020940926	0.0
25	4.901172315861842E-4	0.0	0.0	12.319185728142665	0.0
26	4.901172315861842E-4	0.0	0.0	12.648054390536995	0.0
27	4.901172315861842E-4	0.0	0.0	13.030657723957955	0.0
28	4.901172315861842E-4	0.0	0.0	13.39276524587622	0.0
29	4.901172315861842E-4	0.0	0.0	13.822776282425153	0.0
30	4.901172315861842E-4	0.0	0.0	14.352949458665517	0.0
31	5.346733435485646E-4	0.0	0.0	14.76233101537587	0.0
32	5.792294555109449E-4	0.0	0.0	15.162756793581782	0.0
33	5.792294555109449E-4	0.0	0.0	15.5516870949014	0.0
34	6.237855674733253E-4	0.0	0.0	15.991054914962433	0.0
35	6.237855674733253E-4	0.0	0.0	16.46027533003826	0.0
36	6.237855674733253E-4	0.0	0.0	16.88632087262254	0.0
37	6.237855674733253E-4	0.0	0.0	17.300960050544454	0.0
38	6.683416794357058E-4	0.0	0.0	17.80627091630981	0.0
39	6.683416794357058E-4	0.0	0.0	18.749880255449103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	7.034723E-4	45.000004	26
CGTTTTT	11615	0.0	43.566513	1
ATACGTC	40	3.4601908E-7	39.375004	43
TCTTGCG	570	0.0	38.289474	1
TTGGGAC	4310	0.0	38.26566	5
TTTCGCG	535	0.0	37.850468	1
ACACGCG	655	0.0	37.78626	36
TTTGGGA	12005	0.0	37.55935	4
GCGATCT	470	0.0	37.340427	9
TTCTTCG	660	0.0	37.159092	1
TGGGCGA	2270	0.0	36.971367	6
GGGCGAT	3230	0.0	36.919506	7
CGAGATA	745	0.0	36.84564	19
TTGGGAT	7135	0.0	36.58024	5
TTTGGGC	4740	0.0	36.503162	4
CGGGTAT	275	0.0	36.0	6
TATCTCG	50	4.8825314E-8	36.0	1
TTGGGAG	5670	0.0	35.95238	5
TTCGTTG	985	0.0	35.862946	1
CACGCGG	705	0.0	35.74468	37
>>END_MODULE