##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1566020
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17376278719301	33.0	31.0	34.0	30.0	34.0
2	32.35770232819504	34.0	31.0	34.0	30.0	34.0
3	32.432189244070955	34.0	31.0	34.0	30.0	34.0
4	35.94575228924279	37.0	35.0	37.0	35.0	37.0
5	35.8903091914535	37.0	35.0	37.0	35.0	37.0
6	35.39547770781982	37.0	35.0	37.0	33.0	37.0
7	35.78288591461156	37.0	35.0	37.0	35.0	37.0
8	35.82930103063818	37.0	35.0	37.0	35.0	37.0
9	37.65164748853782	39.0	38.0	39.0	35.0	39.0
10	37.25791752340328	39.0	37.0	39.0	34.0	39.0
11	37.15589200648778	39.0	37.0	39.0	34.0	39.0
12	36.77261848507682	39.0	35.0	39.0	33.0	39.0
13	36.59003461003052	39.0	35.0	39.0	33.0	39.0
14	37.45099998722877	40.0	36.0	41.0	32.0	41.0
15	37.58183611958979	40.0	36.0	41.0	32.0	41.0
16	37.76858980089654	40.0	36.0	41.0	33.0	41.0
17	37.679622227046906	40.0	36.0	41.0	33.0	41.0
18	37.694954725993284	40.0	36.0	41.0	33.0	41.0
19	37.71543019884803	40.0	36.0	41.0	33.0	41.0
20	37.559496047304634	40.0	35.0	41.0	32.0	41.0
21	37.461831905084225	39.0	35.0	41.0	32.0	41.0
22	37.602055529303584	39.0	35.0	41.0	33.0	41.0
23	37.51608919426317	39.0	35.0	41.0	33.0	41.0
24	37.48338590822595	39.0	35.0	41.0	33.0	41.0
25	37.424488192998815	39.0	35.0	41.0	33.0	41.0
26	37.33837690450952	39.0	35.0	41.0	32.0	41.0
27	37.22093651422076	39.0	35.0	41.0	32.0	41.0
28	37.09445920230904	39.0	35.0	41.0	32.0	41.0
29	36.982936360966015	39.0	35.0	41.0	31.0	41.0
30	36.960540095273366	39.0	35.0	41.0	31.0	41.0
31	36.87470913526008	39.0	35.0	41.0	31.0	41.0
32	36.68643567770526	39.0	35.0	41.0	31.0	41.0
33	36.63735584475294	39.0	35.0	41.0	31.0	41.0
34	36.387316892504565	39.0	35.0	41.0	30.0	41.0
35	36.29814114762264	39.0	35.0	41.0	30.0	41.0
36	36.160283393570964	38.0	35.0	41.0	30.0	41.0
37	36.109309587361594	38.0	35.0	40.0	30.0	41.0
38	36.02154250903565	38.0	35.0	40.0	29.0	41.0
39	35.9497145630324	38.0	35.0	40.0	29.0	41.0
40	35.74582380812505	38.0	35.0	40.0	28.0	41.0
41	35.6160451335232	38.0	34.0	40.0	28.0	41.0
42	35.61818623006092	38.0	34.0	40.0	28.0	41.0
43	35.565517681766515	38.0	34.0	40.0	28.0	41.0
44	35.43778623516941	38.0	34.0	40.0	28.0	41.0
45	35.42816566838227	38.0	34.0	40.0	28.0	41.0
46	35.31553875429432	37.0	34.0	40.0	28.0	41.0
47	35.014153075950496	37.0	34.0	40.0	26.0	41.0
48	34.937550605994815	37.0	34.0	40.0	26.0	41.0
49	34.971582738406916	37.0	34.0	40.0	27.0	41.0
50	34.84498218413558	37.0	34.0	40.0	26.0	41.0
51	33.88151364605816	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	28.0
10	45.0
11	41.0
12	38.0
13	42.0
14	62.0
15	61.0
16	168.0
17	278.0
18	532.0
19	996.0
20	1880.0
21	3343.0
22	5323.0
23	7120.0
24	8798.0
25	11461.0
26	14823.0
27	17613.0
28	20301.0
29	24543.0
30	30533.0
31	39355.0
32	51858.0
33	73755.0
34	124536.0
35	127613.0
36	131235.0
37	191919.0
38	303827.0
39	373765.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.703579775481792	4.667054060612253	50.15006194046053	15.479304223445423
2	20.696862109040755	8.200597693516047	49.029258885582564	22.07328131186064
3	22.186306688292614	8.431693081825264	47.52282857179346	21.85917165808866
4	18.47875506059948	5.599162207379216	50.61889375614616	25.303188975875145
5	21.158478180355296	6.1225271707896445	48.08629519418653	24.632699454668522
6	21.587910754651922	9.784102374171466	50.16781394873628	18.460172922440325
7	81.80521321566775	2.8248042809159526	10.886514859324912	4.483467644091391
8	83.1745443864063	5.778342549903577	7.2140841113140315	3.833028952376087
9	78.19778802314147	5.182117725188695	8.687373085912057	7.932721165757781
10	43.70372025900052	26.797869759006907	15.547949579188005	13.950460402804563
11	35.60950690284926	23.22888596569648	22.81477886617029	18.346828265283968
12	30.516596212053486	21.212947471935227	27.705010153127034	20.565446162884253
13	28.917638344337877	21.024891125272983	29.98231184786912	20.07515868252002
14	21.61064354222807	24.511372779402564	31.548447657118047	22.329536021251325
15	19.937740258745098	22.24486277314466	35.00970613402128	22.807690834088966
16	26.619711114800577	22.467912287199397	28.797524935824576	22.11485166217545
17	26.372843258706784	24.093753591908147	27.57467976143344	21.958723387951622
18	27.217085350123245	22.48413174799811	28.119691957957116	22.179090943921533
19	26.306305155745136	24.70511232295884	26.337466954445027	22.651115566850997
20	28.0770360531794	27.0797307824932	26.67577681000243	18.167456354324976
21	26.669135770935238	26.953487184071722	28.325819593619496	18.051557451373547
22	24.731868047662225	21.22527170789645	31.17009999872288	22.872760245718446
23	23.969170253253473	24.826758278949182	30.284606837715994	20.919464630081354
24	24.291196791867282	25.125158043958567	28.182207123791525	22.401438040382626
25	25.303444400454655	26.93445805289843	24.697130304849235	23.064967241797678
26	23.181760130777384	24.22133816937204	27.000038313686925	25.596863386163648
27	23.372817716248832	24.663031123485013	28.485332243521793	23.47881891674436
28	19.73365601971878	27.16191364094967	28.062859989016744	25.041570350314814
29	22.753860103957805	27.73297914458308	26.877626083958063	22.63553466750105
30	25.457976271056566	25.445332754370952	28.357364529188644	20.739326445383842
31	27.40016091748509	23.97925952414401	26.826605024201477	21.793974534169422
32	27.926718688139356	25.893922172130623	25.456507579724395	20.72285156000562
33	27.01242640579303	23.411706108478818	25.54482062808904	24.03104685763911
34	23.805762378513684	25.414937229409585	29.123446699275874	21.655853692800857
35	23.407555459061825	27.61714409777653	27.113382970843286	21.861917472318364
36	24.835953563811444	28.53622559098862	24.44611179933845	22.181709045861485
37	23.305321771114034	29.733272882849516	24.93473901993589	22.02666632610056
38	25.692456035044252	27.123663810168452	24.90517362485792	22.278706529929377
39	24.250711996015376	25.25037994406202	24.71475460083524	25.784153459087367
40	26.793719109589915	23.09089283661767	27.622508013946184	22.492880039846234
41	23.122501628331694	24.193241465626237	25.633772237902452	27.050484668139614
42	23.47856349216485	23.5057023537375	28.154301988480352	24.8614321656173
43	22.57193394720374	23.397530044316163	27.23700846732481	26.79352754115529
44	24.31246088811126	23.216370161300624	27.446584334810538	25.024584615777577
45	23.43494974521398	22.47365934023831	26.242576723158066	27.848814191389636
46	23.692864714371463	24.653069564884227	26.203816043217838	25.45024967752647
47	20.51308412408526	26.145323814510668	30.683452318616624	22.658139742787448
48	21.73018224543748	27.142629085196866	27.721421182360377	23.405767487005274
49	24.429700770105107	23.50250954649366	29.142411974304288	22.925377709096946
50	22.437197481513646	24.46303367773081	27.527043077355334	25.57272576340021
51	21.83484246689059	23.91419011251453	26.184595343609917	28.06637207698497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	1271.5
2	2232.0
3	5094.0
4	7956.0
5	5389.5
6	2823.0
7	2758.5
8	2694.0
9	2820.0
10	2946.0
11	3000.5
12	3055.0
13	2941.0
14	2827.0
15	2818.0
16	2809.0
17	2796.0
18	2783.0
19	3107.0
20	3431.0
21	3540.5
22	3650.0
23	4472.5
24	5295.0
25	6336.5
26	8875.0
27	10372.0
28	12607.5
29	14843.0
30	19317.5
31	23792.0
32	25753.5
33	27715.0
34	32777.0
35	37839.0
36	39968.0
37	42097.0
38	46460.0
39	50823.0
40	56460.5
41	62098.0
42	67318.5
43	72539.0
44	79122.0
45	85705.0
46	111173.0
47	136641.0
48	134110.0
49	131579.0
50	131931.5
51	132284.0
52	125176.5
53	118069.0
54	113215.0
55	108361.0
56	104267.0
57	100173.0
58	98956.5
59	97740.0
60	86621.5
61	75503.0
62	68257.5
63	61012.0
64	51778.5
65	42545.0
66	36492.5
67	30440.0
68	25672.0
69	20904.0
70	16667.0
71	12430.0
72	10492.0
73	8554.0
74	7265.0
75	4742.5
76	3509.0
77	2559.5
78	1610.0
79	982.0
80	354.0
81	277.0
82	200.0
83	122.0
84	44.0
85	45.5
86	47.0
87	33.5
88	20.0
89	14.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1566020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.572067933089862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62676617980972	18.337339681970974
2	9.946069079774428	4.887909701908436
3	3.573033807030207	2.6339048830071885
4	1.7994467847869475	1.7686451455106005
5	1.0855914166359972	1.3337613018579493
6	0.7136512131039711	1.052153165335366
7	0.5445579662245861	0.936664073770305
8	0.440041225541366	0.8650178309890051
9	0.3691920550833555	0.816463103207874
>10	5.240534825916006	32.76676040627765
>50	1.525030874143226	24.856130807322497
>100	0.12559635833895053	5.255753633695076
>500	0.005506312145899002	0.8804904631032907
>1k	0.004195285444494477	1.9309453519102608
>5k	7.866160208427146E-4	1.678060450133558
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	9214	0.5883705188950332	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	8859	0.5657015874637616	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	7972	0.509061186958021	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4431	0.28294657794919603	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3270	0.20880959374720628	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT	2254	0.14393175055235566	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	2125	0.13569430786324568	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1866	0.11915556634014891	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	1804	0.11519648535778598	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1780	0.11366393788074225	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	1768	0.11289766414222041	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTC	1649	0.10529878290187866	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTA	1577	0.10070114047074749	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.27712289753643E-4	0.0	0.0	0.15427644602240073	0.0
2	1.27712289753643E-4	0.0	0.0	0.6512688215987025	0.0
3	1.27712289753643E-4	0.0	0.0	0.8955824318974215	0.0
4	1.27712289753643E-4	0.0	0.0	1.315755865186907	0.0
5	1.27712289753643E-4	0.0	0.0	2.3997777806158287	0.0
6	1.27712289753643E-4	0.0	0.0	2.8545612444285515	0.0
7	1.27712289753643E-4	0.0	0.0	3.3338654678739736	0.0
8	1.27712289753643E-4	0.0	0.0	3.9578038594653964	0.0
9	1.27712289753643E-4	0.0	0.0	4.243751676223803	0.0
10	1.27712289753643E-4	0.0	0.0	5.39597195438117	0.0
11	1.27712289753643E-4	0.0	0.0	6.333635585752417	0.0
12	1.27712289753643E-4	0.0	0.0	7.77703988454809	0.0
13	1.27712289753643E-4	0.0	0.0	8.0894241452855	0.0
14	1.27712289753643E-4	0.0	0.0	8.233355895837857	0.0
15	1.27712289753643E-4	0.0	0.0	8.525944751663452	0.0
16	1.27712289753643E-4	0.0	0.0	8.903206855595714	0.0
17	1.27712289753643E-4	0.0	0.0	9.377083306726606	0.0
18	1.27712289753643E-4	0.0	0.0	9.859388768981239	0.0
19	1.27712289753643E-4	0.0	0.0	10.405805800692201	0.0
20	1.27712289753643E-4	0.0	0.0	10.840091441999464	0.0
21	1.27712289753643E-4	0.0	0.0	11.396917025325347	0.0
22	1.27712289753643E-4	0.0	0.0	12.010766146026231	0.0
23	1.27712289753643E-4	0.0	0.0	12.545305934790106	0.0
24	1.27712289753643E-4	0.0	0.0	12.972567400160917	0.0
25	1.27712289753643E-4	0.0	0.0	13.353086167481896	0.0
26	1.27712289753643E-4	0.0	0.0	13.703911827435155	0.0
27	1.27712289753643E-4	0.0	0.0	14.146626479866157	0.0
28	1.27712289753643E-4	0.0	0.0	14.53270073179142	0.0
29	1.27712289753643E-4	0.0	0.0	14.99738189806005	0.0
30	1.27712289753643E-4	0.0	0.0	15.54424592278515	0.0
31	1.27712289753643E-4	0.0	0.0	15.934343111837652	0.0
32	1.27712289753643E-4	0.0	0.0	16.34762008148044	0.0
33	1.27712289753643E-4	0.0	0.0	16.774051416967854	0.0
34	1.915684346304645E-4	0.0	0.0	17.234326509239985	0.0
35	1.915684346304645E-4	0.0	0.0	17.72059105247698	0.0
36	2.55424579507286E-4	0.0	0.0	18.11592444540938	0.0
37	2.55424579507286E-4	0.0	0.0	18.557298118797974	0.0
38	2.55424579507286E-4	0.0	0.0	19.107929656070805	0.0
39	2.55424579507286E-4	0.0	0.0	20.229371272397543	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.166007E-6	45.000004	5
CCGTACA	20	7.0340704E-4	45.0	43
ACATACG	200	0.0	41.625	17
TTTACGG	160	0.0	40.78125	2
CCGATGA	910	0.0	40.054943	18
TTCGTAC	45	1.9290383E-8	40.0	33
TTTCGCG	480	0.0	39.375004	1
CTATCGG	40	3.4592995E-7	39.375	2
ACTACGG	40	3.4592995E-7	39.375	2
CGGTCTA	40	3.4592995E-7	39.375	31
TTTGGGA	6810	0.0	39.31718	4
TCGTTTG	475	0.0	39.315792	1
CGTTCAT	315	0.0	39.285713	17
TACGGAA	185	0.0	38.91892	5
TTGGGAC	2685	0.0	38.79888	5
TACGGCT	1400	0.0	38.732143	7
TCGTTGA	105	0.0	38.57143	24
ATCGACG	35	6.2497384E-6	38.57143	27
CGTTTTT	2385	0.0	38.490566	1
TTACGGG	325	0.0	38.076923	3
>>END_MODULE