FastQCFastQC Report
Sat 14 Jan 2017
SRR2934355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980242
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59380.2998623400574273No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG39130.19760211125710897No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC31680.1599804468342758No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT30870.1558900376822631No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC28970.14629525078248012No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC28180.1423058393873072No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC20450.10327020636871655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33550.040.6408351
GCCGATG1700.039.7058839
CCGTCGG1150.039.1304362
ACACGCG1500.039.036
TTTGGGC51600.038.1976744
TTGGGCA35500.038.0281685
TTTTGCG16400.037.7286571
CATACGG301.1402201E-437.4999963
TTGTGCG4050.037.222221
TTTGGGA87450.037.178394
TCGTTCA2450.036.73469516
TTTCTCG8950.036.703911
GACCGAT8600.036.6279079
TTGGGAC28900.036.202425
TGGGCGA17300.036.0260126
CGCAATA250.002107475236.00000435
ATACGCT250.002107475236.00000415
CGCCCTA250.002107475236.00000433
CTTGCGG11250.036.02
TCTGGGA42950.035.9895254
Produced by FastQC (version 0.11.5)