##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1980242
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.243983816119446	33.0	31.0	34.0	30.0	34.0
2	32.441208195765974	34.0	31.0	34.0	31.0	34.0
3	32.50666534696264	34.0	31.0	34.0	31.0	34.0
4	35.989115976734155	37.0	35.0	37.0	35.0	37.0
5	35.925242470364736	37.0	35.0	37.0	35.0	37.0
6	35.561406131169825	37.0	35.0	37.0	33.0	37.0
7	35.88619825253681	37.0	35.0	37.0	35.0	37.0
8	35.98130228527624	37.0	35.0	37.0	35.0	37.0
9	37.780226356172626	39.0	38.0	39.0	35.0	39.0
10	37.3057853535073	39.0	37.0	39.0	34.0	39.0
11	37.182715041898916	39.0	37.0	39.0	34.0	39.0
12	36.91975829216833	39.0	35.0	39.0	33.0	39.0
13	36.76985388654518	39.0	35.0	39.0	33.0	39.0
14	37.58653235311644	40.0	37.0	41.0	32.0	41.0
15	37.726271839502445	40.0	37.0	41.0	32.0	41.0
16	37.903114871818694	40.0	37.0	41.0	33.0	41.0
17	37.924918267565275	40.0	36.0	41.0	33.0	41.0
18	37.91103966080913	40.0	36.0	41.0	33.0	41.0
19	37.937025878655234	40.0	36.0	41.0	33.0	41.0
20	37.84213949608179	40.0	36.0	41.0	33.0	41.0
21	37.68198684807211	40.0	36.0	41.0	33.0	41.0
22	37.83725473957223	40.0	36.0	41.0	33.0	41.0
23	37.82392051072546	40.0	36.0	41.0	33.0	41.0
24	37.762682035831986	40.0	36.0	41.0	33.0	41.0
25	37.674283244169146	40.0	35.0	41.0	33.0	41.0
26	37.61201711709983	40.0	35.0	41.0	33.0	41.0
27	37.544869263453656	39.0	35.0	41.0	33.0	41.0
28	37.41671169483326	39.0	35.0	41.0	33.0	41.0
29	37.332387657670125	39.0	35.0	41.0	32.0	41.0
30	37.27946079317579	39.0	35.0	41.0	32.0	41.0
31	37.171824958767665	39.0	35.0	41.0	32.0	41.0
32	37.06113596217028	39.0	35.0	41.0	31.0	41.0
33	36.99893144373264	39.0	35.0	41.0	31.0	41.0
34	36.78920303680055	39.0	35.0	41.0	31.0	41.0
35	36.73384313634394	39.0	35.0	41.0	31.0	41.0
36	36.57279615319744	39.0	35.0	41.0	30.0	41.0
37	36.51808516332852	39.0	35.0	41.0	30.0	41.0
38	36.42798708440686	39.0	35.0	41.0	30.0	41.0
39	36.360034783627455	39.0	35.0	41.0	30.0	41.0
40	36.2095809502071	39.0	35.0	41.0	30.0	41.0
41	36.06104960908818	39.0	35.0	40.0	29.0	41.0
42	36.01760693894989	38.0	35.0	40.0	29.0	41.0
43	36.010528511161766	38.0	35.0	40.0	29.0	41.0
44	35.90251191521036	38.0	35.0	40.0	29.0	41.0
45	35.97458542945761	38.0	35.0	40.0	29.0	41.0
46	35.922302425663126	38.0	35.0	40.0	29.0	41.0
47	35.70488556449161	38.0	34.0	40.0	29.0	41.0
48	35.56972178147923	38.0	34.0	40.0	28.0	41.0
49	35.559497273565555	38.0	34.0	40.0	28.0	41.0
50	35.44079713489563	38.0	34.0	40.0	28.0	41.0
51	34.475653985724975	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	13.0
9	31.0
10	36.0
11	49.0
12	45.0
13	43.0
14	62.0
15	86.0
16	183.0
17	280.0
18	577.0
19	1003.0
20	1644.0
21	2561.0
22	4012.0
23	5998.0
24	8752.0
25	12543.0
26	16860.0
27	20678.0
28	23987.0
29	28475.0
30	35499.0
31	45550.0
32	60338.0
33	87271.0
34	142410.0
35	142557.0
36	159403.0
37	238952.0
38	397839.0
39	542265.0
40	237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.929276320772917	4.8727377764939845	54.40335070158092	15.794635201152182
2	21.58877551329585	4.59953884424227	52.682904412693	21.128781229768887
3	21.630285591357016	4.656249084707829	51.25454363658584	22.458921687349324
4	19.524431862368335	5.080136670164555	50.5828580547226	24.812573412744506
5	18.35083792788962	6.301300548114826	50.873277104515516	24.474584419480045
6	21.87055925487895	6.622877405892814	53.28217460290207	18.224388736326166
7	84.3126244166117	2.4604063543748693	8.712975484814482	4.513993744198942
8	86.7284402613418	2.7861746190617107	6.796593547657307	3.6887915719391873
9	81.90867580831029	4.878494648633854	8.522948205320361	4.689881337735489
10	42.2510481042216	24.246076994629952	17.75964755822773	15.743227342920713
11	29.938765060028018	26.416468290239276	24.794040324364396	18.850726325368313
12	28.808549662112004	22.12997199332203	28.915657783240633	20.145820561325333
13	27.88285472179663	21.03177288432424	30.958892902988627	20.12647949089051
14	21.788852069595535	23.074099024260672	34.10350856107486	21.033540345068936
15	19.803539163395182	25.03875788918728	35.23599640851977	19.92170653889777
16	25.997024606083496	24.297333356226158	29.526037726702093	20.179604310988253
17	26.133725069966196	24.09821627861645	27.86346315248338	21.90459549893397
18	25.858001193793484	23.304727402004403	30.646708836596737	20.190562567605376
19	26.510598199613987	25.425932790032736	27.97723712556344	20.086231884789836
20	24.531648152094544	28.519797075306958	28.09146558854928	18.857089184049222
21	24.81974425348013	25.15732925571723	31.29016554542324	18.732760945379404
22	23.606306703928105	22.250159323961416	32.05507205684962	22.08846191526086
23	22.553152594480878	23.571815969967307	31.824898169011668	22.05013326654015
24	22.038922515530928	26.87489710853522	29.384034880585304	21.70214549534855
25	24.84241824989067	25.7040806123696	27.033362589016896	22.420138548722832
26	21.524338944432046	26.471663564352237	26.92039659799156	25.083600893224162
27	20.887548087556976	26.311329625369023	30.4779415849174	22.323180702156606
28	19.990839503454627	26.589426948827466	28.767746568348716	24.65198697936919
29	21.997058945320823	26.881815454878748	27.93007117311925	23.191054426681184
30	24.34091388830254	25.36604112022672	28.088738649114603	22.204306342356137
31	23.81269562003028	26.0728739214702	28.109594685902028	22.00483577259749
32	25.661914048888974	26.562107055602297	25.486531444136624	22.289447451372105
33	25.42790224629111	25.738823840722496	26.06540008746406	22.767873825522337
34	21.768703017105988	25.898703289799936	29.43650321526359	22.896090477830487
35	21.917472712931048	28.454249531117913	26.40263159755222	23.22564615839882
36	24.258550217599666	27.495376827680655	25.273931165988806	22.97214178873087
37	22.454730280440472	29.485133635181963	25.94753570523199	22.11260037914558
38	23.82284589459268	27.5279486042615	26.325115819177658	22.32408968196816
39	22.308081537509054	25.548089576930494	26.830357097768857	25.313471787791592
40	24.703748329749597	24.50508574204567	27.991932299183635	22.7992336290211
41	21.589886488621087	25.87239337414316	27.54562321170847	24.992096925527285
42	20.357663356296857	27.12885596810895	28.7996618595101	23.713818816084096
43	20.803568452744663	25.745237198281828	29.04892432339078	24.40227002558273
44	22.454730280440472	24.524073320331556	27.91689096585165	25.10430543337632
45	21.7174971543882	24.87074812068424	26.24376212604318	27.16799259888438
46	21.235838852019096	25.439920979355048	27.701462750512313	25.622777418113547
47	19.840150850249614	26.7523363306101	29.643144625757863	23.764368193382428
48	20.35124999873753	28.53570422200923	27.956886077560217	23.156159701693024
49	23.18135864202456	25.864717544623332	27.98410497302855	22.969818840323555
50	21.541710558608493	25.98677333376426	26.58260960024078	25.888906507386473
51	20.91193904583379	26.482571322090937	25.985662358439022	26.619827273636254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	354.0
1	1419.5
2	2485.0
3	6226.0
4	9967.0
5	6819.5
6	3672.0
7	3663.5
8	3655.0
9	3771.0
10	3887.0
11	3920.0
12	3953.0
13	3994.5
14	4036.0
15	4119.0
16	4202.0
17	4082.5
18	3963.0
19	4177.5
20	4392.0
21	5281.5
22	6171.0
23	7525.0
24	8879.0
25	10572.0
26	14887.5
27	17510.0
28	20990.0
29	24470.0
30	29571.5
31	34673.0
32	39678.5
33	44684.0
34	50929.5
35	57175.0
36	62092.5
37	67010.0
38	70620.0
39	74230.0
40	81437.0
41	88644.0
42	96484.5
43	104325.0
44	114190.0
45	124055.0
46	139489.5
47	154924.0
48	172781.5
49	190639.0
50	189366.5
51	188094.0
52	169312.0
53	150530.0
54	138681.0
55	126832.0
56	121408.5
57	115985.0
58	106321.5
59	96658.0
60	84977.0
61	73296.0
62	63266.5
63	53237.0
64	47359.5
65	41482.0
66	35479.0
67	29476.0
68	24674.5
69	19873.0
70	15837.0
71	11801.0
72	10178.0
73	8555.0
74	7075.5
75	4107.0
76	2618.0
77	1939.0
78	1260.0
79	840.5
80	421.0
81	283.5
82	146.0
83	103.0
84	60.0
85	68.5
86	77.0
87	46.0
88	15.0
89	10.0
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1980242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.553743103201043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4675864589695	18.039042443257486
2	10.534635518856449	5.173294684317165
3	4.0346840327565765	2.971997857286764
4	2.0454221383577753	2.0089107889134783
5	1.1736826230685817	1.4409150805758548
6	0.8174324574962947	1.2042615939349397
7	0.5904465705378766	1.0148371386407167
8	0.4453301527310685	0.8747617733014353
9	0.35679032239458663	0.7884484126006462
>10	4.572012199762103	28.446231802458595
>50	1.80769508559449	30.031377720597675
>100	0.14698530314197494	5.7063759241501835
>500	0.005212240237742064	0.8826013257207915
>1k	0.0018764064855871427	1.1129653397422266
>5k	2.0848960950968251E-4	0.3039781145020625
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5938	0.2998623400574273	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3913	0.19760211125710897	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	3168	0.1599804468342758	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3087	0.1558900376822631	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2897	0.14629525078248012	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2818	0.1423058393873072	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2045	0.10327020636871655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5149663525973087E-4	0.0	0.0	0.17109020008665607	0.0
2	1.5149663525973087E-4	0.0	0.0	0.6368918546319087	0.0
3	1.5149663525973087E-4	0.0	0.0	0.938420657677193	0.0
4	1.5149663525973087E-4	0.0	0.0	1.3115063714434902	0.0
5	1.5149663525973087E-4	0.0	0.0	1.9861713871334918	0.0
6	1.5149663525973087E-4	0.0	0.0	2.55589973346692	0.0
7	1.5149663525973087E-4	0.0	0.0	3.025842296042605	0.0
8	1.5149663525973087E-4	0.0	0.0	3.70984960424029	0.0
9	1.5149663525973087E-4	0.0	0.0	4.104447840213469	0.0
10	2.0199551367964117E-4	0.0	0.0	4.769770563395787	0.0
11	2.0199551367964117E-4	0.0	0.0	6.004518639641014	0.0
12	2.0199551367964117E-4	0.0	0.0	6.975763568291148	0.0
13	2.0199551367964117E-4	0.0	0.0	7.2976939182180764	0.0
14	2.0199551367964117E-4	0.0	0.0	7.446413115164712	0.0
15	2.0199551367964117E-4	0.0	0.0	7.621038236740762	0.0
16	2.0199551367964117E-4	0.0	0.0	8.023059807841667	0.0
17	2.0199551367964117E-4	0.0	0.0	8.576931506351244	0.0
18	2.0199551367964117E-4	0.0	0.0	9.206905014639625	0.0
19	2.524943920995515E-4	0.0	0.0	9.650032672774337	0.0
20	2.524943920995515E-4	0.0	0.0	10.165020234900583	0.0
21	3.0299327051946175E-4	0.0	0.0	10.865944667368938	0.0
22	3.0299327051946175E-4	0.0	0.0	11.61898394236664	0.0
23	3.534921489393721E-4	0.0	0.0	12.30632417654004	0.0
24	3.534921489393721E-4	0.0	0.0	12.82949255697031	0.0
25	4.544899057791926E-4	0.0	0.0	13.310140881771016	0.0
26	5.04988784199103E-4	0.0	0.0	13.736856404419258	0.0
27	5.04988784199103E-4	0.0	0.0	14.24765255963665	0.0
28	5.554876626190133E-4	0.0	0.0	14.714262196236621	0.0
29	6.059865410389235E-4	0.0	0.0	15.285101517895287	0.0
30	6.059865410389235E-4	0.0	0.0	16.02576856768011	0.0
31	6.564854194588338E-4	0.0	0.0	16.553027357262394	0.0
32	6.564854194588338E-4	0.0	0.0	17.06634845640078	0.0
33	6.564854194588338E-4	0.0	0.0	17.57537715087348	0.0
34	6.564854194588338E-4	0.0	0.0	18.148438423182622	0.0
35	6.564854194588338E-4	0.0	0.0	18.76937263223384	0.0
36	6.564854194588338E-4	0.0	0.0	19.300469336576036	0.0
37	6.564854194588338E-4	0.0	0.0	19.82484969008838	0.0
38	6.564854194588338E-4	0.0	0.0	20.48067862412776	0.0
39	6.564854194588338E-4	0.0	0.0	21.838037977176526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3355	0.0	40.640835	1
GCCGATG	170	0.0	39.705883	9
CCGTCGG	115	0.0	39.130436	2
ACACGCG	150	0.0	39.0	36
TTTGGGC	5160	0.0	38.197674	4
TTGGGCA	3550	0.0	38.028168	5
TTTTGCG	1640	0.0	37.728657	1
CATACGG	30	1.1402201E-4	37.499996	3
TTGTGCG	405	0.0	37.22222	1
TTTGGGA	8745	0.0	37.17839	4
TCGTTCA	245	0.0	36.734695	16
TTTCTCG	895	0.0	36.70391	1
GACCGAT	860	0.0	36.627907	9
TTGGGAC	2890	0.0	36.20242	5
TGGGCGA	1730	0.0	36.026012	6
CGCAATA	25	0.0021074752	36.000004	35
ATACGCT	25	0.0021074752	36.000004	15
CGCCCTA	25	0.0021074752	36.000004	33
CTTGCGG	1125	0.0	36.0	2
TCTGGGA	4295	0.0	35.989525	4
>>END_MODULE