##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	850990
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.182287688456974	33.0	31.0	34.0	30.0	34.0
2	32.387607374939776	34.0	31.0	34.0	30.0	34.0
3	32.439651464764566	34.0	31.0	34.0	30.0	34.0
4	35.925596070459115	37.0	35.0	37.0	35.0	37.0
5	35.89091881220696	37.0	35.0	37.0	35.0	37.0
6	35.52227758258029	37.0	35.0	37.0	33.0	37.0
7	35.863261612944925	37.0	35.0	37.0	35.0	37.0
8	35.9383071481451	37.0	35.0	37.0	35.0	37.0
9	37.7086052715073	39.0	38.0	39.0	35.0	39.0
10	37.22414951997086	39.0	37.0	39.0	34.0	39.0
11	37.163751630453945	39.0	37.0	39.0	34.0	39.0
12	36.9649314328018	39.0	37.0	39.0	33.0	39.0
13	36.86858835003937	39.0	37.0	39.0	33.0	39.0
14	38.02131047368359	40.0	37.0	41.0	33.0	41.0
15	38.086657892572184	40.0	37.0	41.0	33.0	41.0
16	38.12553143985241	40.0	37.0	41.0	33.0	41.0
17	38.09062268651805	40.0	37.0	41.0	33.0	41.0
18	38.04915686435798	40.0	37.0	41.0	33.0	41.0
19	38.070081904605225	40.0	37.0	41.0	34.0	41.0
20	37.99911749844299	40.0	37.0	41.0	34.0	41.0
21	37.86268581299428	40.0	36.0	41.0	33.0	41.0
22	37.923902748563435	40.0	36.0	41.0	33.0	41.0
23	37.87989165560112	40.0	36.0	41.0	33.0	41.0
24	37.83728010904946	40.0	36.0	41.0	33.0	41.0
25	37.7644978201859	40.0	36.0	41.0	33.0	41.0
26	37.68075535552709	40.0	36.0	41.0	33.0	41.0
27	37.58824663039519	39.0	36.0	41.0	33.0	41.0
28	37.466075982091446	39.0	36.0	41.0	33.0	41.0
29	37.38389287770714	39.0	36.0	41.0	32.0	41.0
30	37.338008672252315	39.0	36.0	41.0	32.0	41.0
31	37.320229379898706	39.0	35.0	41.0	32.0	41.0
32	37.22261601193904	39.0	35.0	41.0	32.0	41.0
33	37.16185736612651	39.0	35.0	41.0	32.0	41.0
34	37.066954958342635	39.0	35.0	41.0	31.0	41.0
35	36.97702675707118	39.0	35.0	41.0	31.0	41.0
36	36.78928777071411	39.0	35.0	41.0	31.0	41.0
37	36.803356091140905	39.0	35.0	41.0	31.0	41.0
38	36.75700066980811	39.0	35.0	41.0	31.0	41.0
39	36.678792935287134	39.0	35.0	41.0	31.0	41.0
40	36.540823041398845	39.0	35.0	41.0	30.0	41.0
41	36.45995957649326	39.0	35.0	40.0	30.0	41.0
42	36.42056075864581	39.0	35.0	40.0	30.0	41.0
43	36.37090330086135	39.0	35.0	40.0	30.0	41.0
44	36.217921479688364	38.0	35.0	40.0	30.0	41.0
45	36.22465833911092	38.0	35.0	40.0	30.0	41.0
46	36.11253011198721	38.0	35.0	40.0	30.0	41.0
47	35.940873570782266	38.0	35.0	40.0	29.0	41.0
48	35.89580488607387	38.0	35.0	40.0	29.0	41.0
49	35.89969682369946	38.0	35.0	40.0	29.0	41.0
50	35.81804134008625	38.0	35.0	40.0	29.0	41.0
51	34.93159966627105	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	19.0
10	18.0
11	17.0
12	29.0
13	18.0
14	14.0
15	34.0
16	52.0
17	90.0
18	169.0
19	293.0
20	512.0
21	815.0
22	1397.0
23	1960.0
24	3030.0
25	4292.0
26	6112.0
27	7791.0
28	9243.0
29	11111.0
30	14104.0
31	18051.0
32	23543.0
33	32919.0
34	55652.0
35	68585.0
36	72632.0
37	108187.0
38	182877.0
39	227328.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.169837483401682	4.620970869222905	54.94259627022644	17.26659537714897
2	20.55594072785814	4.650348417725238	53.181588502802626	21.612122351614
3	21.797083396984686	4.8680948072245265	50.422566657657555	22.912255138133233
4	19.252400145712638	4.928612557139332	50.159343823076654	25.659643474071377
5	18.00690959940775	6.224632486868236	49.50022914487832	26.2682287688457
6	21.7821596023455	6.93110377325233	53.22753498866027	18.0592016357419
7	82.99216207005958	2.7317594801349014	9.467796331331742	4.808282118473778
8	83.55433083820022	3.628009729844064	7.870832794744945	4.946826637210778
9	77.91854193351273	5.7290919987308895	10.491192610959	5.861173456797377
10	37.211483096158595	28.534295350121624	18.829010916697023	15.425210637022763
11	28.248863088872962	26.316995499359567	26.20442073349863	19.229720678268837
12	27.082221882748332	21.454188650865465	31.00706236265996	20.45652710372625
13	24.58889058625836	22.27593743757271	31.968883300626327	21.166288675542603
14	19.847941808951926	25.926274104278548	30.96511122339863	23.2606728633709
15	17.60290955240367	25.765402648679775	35.075970340427034	21.555717458489525
16	20.14383247746742	24.51697434752465	33.135994547527	22.203198627480933
17	20.76358124067263	24.077368711735744	30.42256665765755	24.736483389934076
18	20.668045452943044	25.29935721923877	30.87004547644508	23.162551851373106
19	23.224362213422015	26.40583320603062	29.276724755872575	21.093079824674792
20	24.630959235713696	26.123808740408233	29.648291989330072	19.596940034548
21	25.16198780244186	25.436961656423694	28.818905040012222	20.582145501122223
22	22.44444705578209	25.0981797670948	28.61890268980834	23.838470487314776
23	22.101317289274842	25.561404951879574	28.997873065488434	23.33940469335715
24	22.549148638644404	24.974794063384998	29.783663732828824	22.692393565141778
25	22.461015993137405	25.403588761325047	28.389170260520103	23.74622498501745
26	20.703298511145842	28.860151118109496	27.441098015252823	22.99545235549184
27	20.684849410686375	28.144279016204653	29.30845250825509	21.862419064853878
28	19.511745143891233	27.740513989588596	30.565576563766903	22.182164302753264
29	21.553014724027307	27.163303916614765	28.727834639654994	22.555846719702934
30	22.720125970927977	27.474118379769447	28.800220919164737	21.00553473013784
31	24.056099366620053	29.049577550852533	24.915686435798307	21.978636646729104
32	23.189344175607236	28.50292012832113	25.525211812124702	22.782523883946933
33	22.920245831325868	27.66319228193046	27.25872219415034	22.15783969259333
34	22.330814698175068	26.23133056792677	27.81196018754627	23.625894546351898
35	21.48286113820374	26.405715695836612	27.393623896873052	24.717799269086594
36	21.204126958013607	30.095653297923597	27.08903747400087	21.611182270061928
37	22.933289462860902	28.194338358852633	27.740866520170627	21.13150565811584
38	23.2480992726119	27.113832124936838	26.88022185924629	22.757846743204972
39	23.56866708186935	25.468571898612204	28.17013125888671	22.792629760631737
40	23.167017238745462	26.044489359451934	28.716201130448066	22.07229227135454
41	20.07908436056828	26.27551440087428	28.560735143773723	25.084666094783724
42	20.701065817459664	26.22063714027192	28.275772923301094	24.80252411896732
43	21.69167675295832	25.73402742687928	28.643579830550298	23.9307159896121
44	22.464306278569666	25.627680701300836	27.640982855262692	24.267030164866803
45	23.27947449441239	25.45223798164491	26.13649984136124	25.131787682581464
46	23.29862865603591	27.02934229544413	26.94673262905557	22.72529641946439
47	20.64031304715684	26.126393964676435	29.95111575929212	23.282177228874605
48	20.266160589431134	24.506633450451826	31.013407913136465	24.213798046980575
49	21.593790761348547	23.989353576422754	30.83408735707823	23.582768305150474
50	21.202716835685496	23.182176053772665	29.776965651770293	25.838141458771545
51	20.151940680854064	23.743522250555234	27.894452343740824	28.21008472484988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	948.5
2	1649.0
3	3158.5
4	4668.0
5	3263.5
6	1859.0
7	1791.5
8	1724.0
9	1801.5
10	1879.0
11	1859.5
12	1840.0
13	1876.5
14	1913.0
15	1790.0
16	1667.0
17	1805.5
18	1944.0
19	2108.5
20	2273.0
21	2834.5
22	3396.0
23	3498.5
24	3601.0
25	4239.5
26	5716.5
27	6555.0
28	9251.5
29	11948.0
30	14041.5
31	16135.0
32	18814.5
33	21494.0
34	22410.0
35	23326.0
36	25469.0
37	27612.0
38	31068.5
39	34525.0
40	38086.0
41	41647.0
42	46470.0
43	51293.0
44	55744.0
45	60195.0
46	67554.0
47	74913.0
48	83180.5
49	91448.0
50	87236.0
51	83024.0
52	72391.0
53	61758.0
54	54171.5
55	46585.0
56	41620.5
57	36656.0
58	36007.5
59	35359.0
60	31846.5
61	28334.0
62	25370.5
63	22407.0
64	18619.5
65	14832.0
66	12681.5
67	10531.0
68	8051.0
69	5571.0
70	5031.0
71	4491.0
72	3723.0
73	2955.0
74	2435.5
75	1401.0
76	886.0
77	696.5
78	507.0
79	351.5
80	196.0
81	168.5
82	141.0
83	136.0
84	131.0
85	81.5
86	32.0
87	34.0
88	36.0
89	22.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	850990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.109637995087926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34536067816349	18.105822639456534
2	12.488086153151775	6.5211881742052595
3	5.209293850672036	4.080383299532534
4	2.716547443551231	2.837122813904168
5	1.579455907313812	2.061951098458339
6	1.0806917431000735	1.6929882117974093
7	0.7413477932913758	1.3549425757906568
8	0.5271265059522854	1.1010465798423787
9	0.41485269441344647	0.974848830517973
>10	4.344850741543156	27.908346213899566
>50	1.4039565526551245	24.61160840075731
>100	0.14025948098889227	6.08659276180457
>500	0.005446970135490962	0.9786997493424876
>1k	0.002723485067745481	1.6844586506908181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	3164	0.3718022538455211	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2701	0.31739503401920116	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	0.3059965452002961	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2577	0.3028237699620442	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2125	0.24970916226982692	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1042	0.12244562215772219	No Hit
GGTTGGGAGTGCCCAGTCACCACGTGTAGTCCTGTTCTGTATTGAAAGACT	923	0.10846190907061189	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	906	0.10646423577245327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33972197088097394	0.0
2	0.0	0.0	0.0	1.372519066028978	0.0
3	0.0	0.0	0.0	2.0073091340673805	0.0
4	0.0	0.0	0.0	2.7822888635589136	0.0
5	0.0	0.0	0.0	3.9437596211471346	0.0
6	0.0	0.0	0.0	4.733898165665871	0.0
7	0.0	0.0	0.0	5.4216853312024815	0.0
8	0.0	0.0	0.0	6.470463812735755	0.0
9	0.0	0.0	0.0	6.971997320767577	0.0
10	0.0	0.0	0.0	7.993865967872713	0.0
11	0.0	0.0	0.0	9.822442096851901	0.0
12	0.0	0.0	0.0	11.448195630970988	0.0
13	0.0	0.0	0.0	11.963007790925863	0.0
14	0.0	0.0	0.0	12.156899611041258	0.0
15	0.0	0.0	0.0	12.465128849927732	0.0
16	0.0	0.0	0.0	13.208380827036745	0.0
17	0.0	0.0	0.0	14.278781184267736	0.0
18	0.0	0.0	0.0	15.457643450569337	0.0
19	0.0	0.0	0.0	16.158709268029	0.0
20	1.1751019400933031E-4	0.0	0.0	16.962126464470792	0.0
21	1.1751019400933031E-4	0.0	0.0	17.984700172739984	0.0
22	1.1751019400933031E-4	0.0	0.0	19.032303552333165	0.0
23	1.1751019400933031E-4	0.0	0.0	19.96862477819951	0.0
24	1.1751019400933031E-4	0.0	0.0	20.709409041234327	0.0
25	1.1751019400933031E-4	0.0	0.0	21.360415516046018	0.0
26	1.1751019400933031E-4	0.0	0.0	21.919058978366373	0.0
27	1.1751019400933031E-4	0.0	0.0	22.5037897037568	0.0
28	2.3502038801866061E-4	0.0	0.0	23.12823887472238	0.0
29	2.3502038801866061E-4	0.0	0.0	23.76349898353682	0.0
30	2.3502038801866061E-4	0.0	0.0	24.489359451932454	0.0
31	2.3502038801866061E-4	0.0	0.0	25.146006416056593	0.0
32	2.3502038801866061E-4	0.0	0.0	25.778916320990845	0.0
33	2.3502038801866061E-4	0.0	0.0	26.405833206030625	0.0
34	2.3502038801866061E-4	0.0	0.0	27.02205666341555	0.0
35	2.3502038801866061E-4	0.0	0.0	27.66460240425857	0.0
36	2.3502038801866061E-4	0.0	0.0	28.28881655483613	0.0
37	2.3502038801866061E-4	0.0	0.0	28.904099930668984	0.0
38	2.3502038801866061E-4	0.0	0.0	29.558396690912936	0.0
39	2.3502038801866061E-4	0.0	0.0	30.191776636623228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	20	7.032252E-4	45.000004	30
ACACGGC	20	7.032252E-4	45.000004	29
TTCAACG	30	2.1648339E-6	45.000004	18
GCGCATT	20	7.032252E-4	45.000004	9
TACGATC	20	7.032252E-4	45.000004	12
CTCGGAC	20	7.032252E-4	45.000004	27
ATAAGCG	20	7.032252E-4	45.000004	33
CGCACGA	30	2.1648339E-6	45.000004	39
CGGTCAT	20	7.032252E-4	45.000004	30
CGACTCG	20	7.032252E-4	45.000004	30
CGGTACG	20	7.032252E-4	45.000004	22
CGGTACA	30	2.1648339E-6	45.000004	14
TAAGCGG	20	7.032252E-4	45.000004	34
GCGTCTA	20	7.032252E-4	45.000004	12
TATCCGA	40	6.8121153E-9	45.000004	25
CGTATGG	55	1.8189894E-12	45.0	2
CGAAACA	25	3.8899485E-5	45.0	42
AAGCGGT	25	3.8899485E-5	45.0	22
ATTCACG	25	3.8899485E-5	45.0	13
TGCGATG	35	1.2114288E-7	45.0	1
>>END_MODULE