FastQCFastQC Report
Sat 14 Jan 2017
SRR2934350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727340
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC56270.7736409382132153TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT52140.7168586905711222TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC43210.5940825473643688TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC37130.5104902796491325TruSeq Adapter, Index 23 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG19390.2665878406247422TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.2040311271207413No Hit
TTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT14330.19701927571699618No Hit
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG11670.16044765859158028TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG11670.16044765859158028TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC10000.13748728242637556No Hit
TTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG8230.11315203343690708TruSeq Adapter, Index 20 (95% over 22bp)
TTGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7920.10888992768168944No Hit
TTTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC7310.10050320345368054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG551.8189894E-1245.0000041
AACGATA1150.045.00000430
CTTCGGC207.0315774E-445.017
CGGGTAA453.8380676E-1045.06
AGCGTAG253.889387E-545.041
GAGTGCG453.8380676E-1045.032
AGTCCGG207.0315774E-445.030
CCTAGGT253.889387E-545.013
TCCGACA207.0315774E-445.015
TTGCTAG406.8084773E-945.01
GGCGTCA453.8380676E-1045.036
TTCACGG351.2111013E-745.02
TCCCTCC207.0315774E-445.016
TTCCGCG900.045.01
CACGAGC207.0315774E-445.041
CGACACA207.0315774E-445.017
TAGCCGA207.0315774E-445.045
TCCTACG253.889387E-545.020
TCGGCAG207.0315774E-445.019
AGTATCC207.0315774E-445.017
Produced by FastQC (version 0.11.5)