##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727340
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87189759947205	31.0	31.0	34.0	30.0	34.0
2	32.05655539362609	33.0	31.0	34.0	30.0	34.0
3	32.12397778205516	33.0	31.0	34.0	30.0	34.0
4	35.70923364588776	37.0	35.0	37.0	35.0	37.0
5	35.698366651084775	37.0	35.0	37.0	35.0	37.0
6	35.38686034041851	37.0	35.0	37.0	33.0	37.0
7	35.79227596447328	37.0	35.0	37.0	35.0	37.0
8	35.895515164847254	37.0	35.0	37.0	35.0	37.0
9	37.69828828333379	39.0	38.0	39.0	35.0	39.0
10	36.93644925344405	39.0	37.0	39.0	33.0	39.0
11	36.79804355597107	39.0	37.0	39.0	32.0	39.0
12	36.727207358319355	39.0	35.0	39.0	32.0	39.0
13	36.64962190997333	39.0	35.0	39.0	32.0	39.0
14	37.700904666318365	39.0	37.0	41.0	33.0	41.0
15	37.72334396568318	39.0	37.0	41.0	33.0	41.0
16	37.76755162647455	39.0	37.0	41.0	33.0	41.0
17	37.725710121813734	39.0	36.0	41.0	33.0	41.0
18	37.70625292160475	39.0	36.0	41.0	33.0	41.0
19	37.743777325597385	39.0	37.0	41.0	33.0	41.0
20	37.72093106387659	39.0	36.0	41.0	33.0	41.0
21	37.547698462892185	39.0	36.0	41.0	32.0	41.0
22	37.65941650397338	39.0	36.0	41.0	33.0	41.0
23	37.60653064591525	39.0	36.0	41.0	33.0	41.0
24	37.56300354717189	39.0	36.0	41.0	33.0	41.0
25	37.490595869882036	39.0	36.0	41.0	32.0	41.0
26	37.43507988011109	39.0	36.0	40.0	32.0	41.0
27	37.35483130310446	39.0	36.0	40.0	32.0	41.0
28	37.22924079522644	39.0	36.0	40.0	32.0	41.0
29	37.20273049742899	39.0	36.0	40.0	32.0	41.0
30	37.17987048697996	39.0	36.0	40.0	31.0	41.0
31	37.10216267495257	39.0	35.0	40.0	31.0	41.0
32	37.00883218302307	39.0	35.0	40.0	31.0	41.0
33	36.96603651662221	39.0	35.0	40.0	31.0	41.0
34	36.86617812852311	39.0	35.0	40.0	31.0	41.0
35	36.82654466961806	39.0	35.0	40.0	31.0	41.0
36	36.58310693760827	39.0	35.0	40.0	30.0	41.0
37	36.48325129925482	39.0	35.0	40.0	30.0	41.0
38	36.41274644595375	39.0	35.0	40.0	30.0	41.0
39	36.34982951576979	39.0	35.0	40.0	30.0	41.0
40	36.2791308054005	39.0	35.0	40.0	30.0	41.0
41	36.229580388814036	39.0	35.0	40.0	29.0	41.0
42	36.10600819424203	38.0	35.0	40.0	29.0	41.0
43	35.9577116616713	38.0	35.0	40.0	28.0	41.0
44	35.77627794429015	38.0	35.0	40.0	28.0	41.0
45	35.81313416009019	38.0	35.0	40.0	28.0	41.0
46	35.628903951384494	38.0	34.0	40.0	28.0	41.0
47	35.31308328979569	38.0	34.0	40.0	27.0	41.0
48	35.19169989275992	38.0	34.0	40.0	26.0	41.0
49	35.135365853658534	38.0	34.0	40.0	27.0	41.0
50	34.92227568949872	37.0	34.0	40.0	26.0	41.0
51	33.85001512360107	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	4.0
13	1.0
14	7.0
15	10.0
16	23.0
17	47.0
18	93.0
19	218.0
20	368.0
21	681.0
22	1149.0
23	1807.0
24	2905.0
25	4425.0
26	6002.0
27	7460.0
28	9037.0
29	11424.0
30	14260.0
31	18337.0
32	23865.0
33	31685.0
34	47383.0
35	61279.0
36	71681.0
37	106075.0
38	167998.0
39	139072.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.532598234663293	4.650919789919432	61.66222124453488	10.154260730882394
2	20.09899084334699	3.990705859707977	54.2394203536173	21.670882943327744
3	19.2827288475816	3.7705887205433495	55.04660818874254	21.90007424313251
4	17.59919707427063	4.4725987846124236	53.75079055187395	24.177413589242995
5	16.317403140209528	4.885610581021256	55.76195451920697	23.03503175956224
6	19.142904281353974	5.614705639728325	58.16921934721039	17.073170731707318
7	82.26867764731762	1.1348200291473038	11.138807160337668	5.457695163197404
8	84.38639425853108	1.294855225891605	8.123711056727252	6.195039458850057
9	79.99628784337449	3.454642945527539	11.13303269447576	5.416036516622212
10	36.17359144279154	26.956444028927322	20.12635081254984	16.743613715731296
11	24.79294415266588	28.15492066983804	27.058872054334977	19.993263123161107
12	21.959743723705557	24.89908433469904	32.810377540077546	20.33079440151786
13	22.95391426293068	26.796408832183022	33.19025490142162	17.05942200346468
14	20.399125580883766	28.941622899881757	30.232628481865426	20.426623037369044
15	17.0991558280859	29.12736821843979	33.95317183160558	19.820304121868727
16	18.533148183793	27.831963043418483	30.529738499188824	23.10515027359969
17	20.025572634531304	28.667335771441145	26.80892017488382	24.49817141914373
18	20.29697253004097	27.06780872769269	31.438116974179884	21.19710176808645
19	20.604531580828773	30.836610113564493	28.553908763439384	20.00494954216735
20	22.695713146533947	29.73451205763467	28.324717463634613	19.245057332196772
21	21.711854153490805	30.417273902164048	28.99964253306569	18.871229411279455
22	20.22190447383617	27.90373140484505	26.848928974069896	25.02543514724888
23	19.27626694530756	29.26678032282014	26.40841972117579	25.04853301069651
24	20.255863832595487	28.212252866609838	27.92724173013996	23.604641570654714
25	18.83699507795529	29.687353919762423	26.518684521681745	24.956966480600546
26	20.28074903071466	30.24197761707042	27.796903786399756	21.680369565815163
27	21.679819616685457	29.282453873016745	28.02100805675475	21.01671845354305
28	18.880853521049303	28.7103692908406	28.820359116781695	23.5884180713284
29	19.914070448483514	26.125333406659884	27.85561085599582	26.10498528886078
30	22.51794209035664	28.158082877333847	26.338301207138336	22.985673825171173
31	19.14606648884978	32.13792724173014	25.916490224654222	22.79951604476586
32	19.272142326834768	31.40704484835153	25.30467181785685	24.016141006956857
33	19.30087716886188	29.951604476585914	25.877306349162705	24.870212005389504
34	16.883575769241347	28.96774548354277	28.42728297632469	25.721395770891192
35	20.38042731047378	25.716446228723843	26.88412571837105	27.019000742431327
36	23.622102455522864	29.04405092528941	25.62432974949817	21.709516869689555
37	18.43223251849204	31.42973024995188	28.1432342508318	21.99480298072428
38	15.963648362526467	32.0386614238183	26.296367585998293	25.701322627656943
39	17.116204251106772	28.00437209558116	28.856518272059834	26.022905381252233
40	19.358759314763386	25.299997250254354	31.118459042538564	24.2227843924437
41	19.313938460692388	26.90529875986471	29.17218907251079	24.60857370693211
42	20.857920642340584	29.354084747160886	26.873401710341792	22.914592900156734
43	21.129045563285395	28.751203013721234	26.548656749250693	23.571094673742678
44	19.671405395000964	26.995215442571563	24.483047818076827	28.85033134435065
45	21.371985591332802	24.998212665328456	24.494459262518216	29.135342480820526
46	22.364506283168804	25.20650589820442	28.02815739544092	24.400830423185855
47	18.347402865234965	27.292875409024663	30.73583193554596	23.623889790194408
48	18.608491214562655	26.258558583331045	29.85811312453598	25.27483707757032
49	20.321445266312864	25.1845079330162	29.479335661451316	25.01471113921962
50	19.532268265185472	23.47127890670113	27.94786482250392	29.04858800560948
51	18.17238155470619	23.12989798443644	26.431655071905848	32.26606538895152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	188.5
2	309.0
3	1232.5
4	2156.0
5	1567.0
6	978.0
7	1021.5
8	1065.0
9	1347.5
10	1630.0
11	1719.0
12	1808.0
13	1986.5
14	2165.0
15	2161.0
16	2157.0
17	2163.0
18	2169.0
19	2215.0
20	2261.0
21	2523.0
22	2785.0
23	2922.0
24	3059.0
25	3572.0
26	4527.5
27	4970.0
28	5866.0
29	6762.0
30	7753.0
31	8744.0
32	10811.0
33	12878.0
34	16496.0
35	20114.0
36	22648.5
37	25183.0
38	27309.0
39	29435.0
40	34734.5
41	40034.0
42	44327.0
43	48620.0
44	56808.5
45	64997.0
46	76657.0
47	88317.0
48	101375.0
49	114433.0
50	105260.0
51	96087.0
52	77000.0
53	57913.0
54	45890.5
55	33868.0
56	27027.0
57	20186.0
58	15696.5
59	11207.0
60	9163.0
61	7119.0
62	5650.5
63	4182.0
64	3241.0
65	2300.0
66	1786.5
67	1273.0
68	1052.0
69	831.0
70	709.5
71	588.0
72	400.5
73	213.0
74	212.5
75	160.0
76	108.0
77	72.5
78	37.0
79	28.0
80	19.0
81	14.0
82	9.0
83	7.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	727340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.03242097303357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27822945274671	27.108836287593423
2	13.401437978303521	10.193782616696785
3	5.278430999998811	6.022545296071959
4	2.6107223108299165	3.9716835987669743
5	1.5046882130091697	2.861346777516317
6	0.9763900960436969	2.228068749998073
7	0.6842117342282582	1.8215560097600934
8	0.5267701509183208	1.6027475308603198
9	0.3901965455956574	1.3356107355885778
>10	2.9055671199287083	22.415399250097
>50	0.3404271173188153	8.534545392847033
>100	0.0945630850896301	6.994290699926134
>500	0.005091858427903139	1.3041328359881232
>1k	0.0025459292139515693	2.1058674475056414
>5k	7.274083468433055E-4	1.499586770783543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	5627	0.7736409382132153	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	5214	0.7168586905711222	TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4321	0.5940825473643688	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3713	0.5104902796491325	TruSeq Adapter, Index 23 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1939	0.2665878406247422	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1484	0.2040311271207413	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	1433	0.19701927571699618	No Hit
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1167	0.16044765859158028	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1167	0.16044765859158028	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	1000	0.13748728242637556	No Hit
TTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	823	0.11315203343690708	TruSeq Adapter, Index 20 (95% over 22bp)
TTGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	792	0.10888992768168944	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	731	0.10050320345368054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7937140814474661	0.0
2	0.0	0.0	0.0	2.937140814474661	0.0
3	0.0	0.0	0.0	4.151703467429263	0.0
4	0.0	0.0	0.0	5.609481122996123	0.0
5	0.0	0.0	0.0	7.765006736876839	0.0
6	0.0	0.0	0.0	9.164489784694915	0.0
7	0.0	0.0	0.0	10.300272224819205	0.0
8	0.0	0.0	0.0	12.210383039568839	0.0
9	0.0	0.0	0.0	12.974949817141914	0.0
10	0.0	0.0	0.0	14.834877773805923	0.0
11	0.0	0.0	0.0	18.441856628261885	0.0
12	0.0	0.0	0.0	21.01080650039871	0.0
13	0.0	0.0	0.0	21.85869057112217	0.0
14	0.0	0.0	0.0	22.096406082437376	0.0
15	0.0	0.0	0.0	22.487144939093135	0.0
16	0.0	0.0	0.0	23.662248741991366	0.0
17	0.0	0.0	0.0	25.529326037341544	0.0
18	0.0	0.0	0.0	27.53897764456788	0.0
19	0.0	0.0	0.0	28.850881293480352	0.0
20	0.0	0.0	0.0	30.087441911623173	0.0
21	0.0	0.0	0.0	32.03467429262793	0.0
22	0.0	0.0	0.0	34.06756125058432	0.0
23	0.0	0.0	0.0	36.26914510407787	0.0
24	0.0	0.0	0.0	37.75524513982457	0.0
25	0.0	0.0	0.0	39.06426155580609	0.0
26	0.0	0.0	0.0	40.16897187010201	0.0
27	0.0	0.0	0.0	41.25168421920972	0.0
28	0.0	0.0	0.0	42.44012428850331	0.0
29	0.0	0.0	0.0	43.735117001677345	0.0
30	0.0	0.0	0.0	45.03602166799571	0.0
31	0.0	0.0	0.0	46.321665245964745	0.0
32	0.0	0.0	0.0	47.43558720818324	0.0
33	0.0	0.0	0.0	48.510187807627794	0.0
34	0.0	0.0	0.0	49.51906948607254	0.0
35	0.0	0.0	0.0	50.60095691148569	0.0
36	0.0	0.0	0.0	51.65548436769599	0.0
37	1.3748728242637555E-4	0.0	0.0	52.66134132592735	0.0
38	1.3748728242637555E-4	0.0	0.0	53.64038826408557	0.0
39	1.3748728242637555E-4	0.0	0.0	54.678554733687136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAG	55	1.8189894E-12	45.000004	1
AACGATA	115	0.0	45.000004	30
CTTCGGC	20	7.0315774E-4	45.0	17
CGGGTAA	45	3.8380676E-10	45.0	6
AGCGTAG	25	3.889387E-5	45.0	41
GAGTGCG	45	3.8380676E-10	45.0	32
AGTCCGG	20	7.0315774E-4	45.0	30
CCTAGGT	25	3.889387E-5	45.0	13
TCCGACA	20	7.0315774E-4	45.0	15
TTGCTAG	40	6.8084773E-9	45.0	1
GGCGTCA	45	3.8380676E-10	45.0	36
TTCACGG	35	1.2111013E-7	45.0	2
TCCCTCC	20	7.0315774E-4	45.0	16
TTCCGCG	90	0.0	45.0	1
CACGAGC	20	7.0315774E-4	45.0	41
CGACACA	20	7.0315774E-4	45.0	17
TAGCCGA	20	7.0315774E-4	45.0	45
TCCTACG	25	3.889387E-5	45.0	20
TCGGCAG	20	7.0315774E-4	45.0	19
AGTATCC	20	7.0315774E-4	45.0	17
>>END_MODULE