SRR2934349.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011322
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6998	0.34793036619695905	RNA PCR Primer, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6161	0.30631594543290436	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	5837	0.2902071373952057	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4857	0.24148296493550014	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4570	0.22721374300087205	RNA PCR Primer, Index 38 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	2329	0.11579448740679016	RNA PCR Primer, Index 38 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	20	7.03455E-4	45.0	1
TCCGTCG	20	7.03455E-4	45.0	1
CGTAACA	20	7.03455E-4	45.0	22
CCGCGTA	20	7.03455E-4	45.0	26
TCGACGT	60	0.0	44.999996	26
CGAATAT	255	0.0	43.235294	14
TCGTTTG	570	0.0	42.236843	1
CAACCCG	75	0.0	42.0	23
CGTTCAT	265	0.0	41.603775	17
TGTTTCG	475	0.0	41.210526	1
CGGGTAC	230	0.0	41.086956	6
TCGTTCA	270	0.0	40.833336	16
CGCGACC	565	0.0	40.61947	10
TTCGTTG	875	0.0	39.85714	1
GCGCGAC	575	0.0	39.52174	9
GCCGATT	205	0.0	39.512196	9
TACGAAT	285	0.0	39.473686	12
GGGCGTA	40	3.4599543E-7	39.375	7
CGAGGTA	40	3.4599543E-7	39.375	13
CGCGTAC	40	3.4599543E-7	39.375	18