##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011322
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17528670198009	33.0	31.0	34.0	30.0	34.0
2	32.35899821112681	34.0	31.0	34.0	30.0	34.0
3	32.43243299680508	34.0	31.0	34.0	30.0	34.0
4	35.93240465723539	37.0	35.0	37.0	35.0	37.0
5	35.89510332010489	37.0	35.0	37.0	35.0	37.0
6	35.56014004719284	37.0	35.0	37.0	33.0	37.0
7	35.92660548634182	37.0	35.0	37.0	35.0	37.0
8	36.011453660826064	37.0	35.0	37.0	35.0	37.0
9	37.780921702243596	39.0	38.0	39.0	35.0	39.0
10	37.2095089697224	39.0	37.0	39.0	34.0	39.0
11	37.11559113856459	39.0	37.0	39.0	33.0	39.0
12	37.06325391956136	39.0	37.0	39.0	33.0	39.0
13	37.068105455019136	39.0	37.0	39.0	33.0	39.0
14	38.23333111257173	40.0	38.0	41.0	33.0	41.0
15	38.26392342946579	40.0	38.0	41.0	33.0	41.0
16	38.28972983937928	40.0	38.0	41.0	34.0	41.0
17	38.23408882317202	40.0	37.0	41.0	33.0	41.0
18	38.19845952065358	40.0	37.0	41.0	33.0	41.0
19	38.242898451864	40.0	37.0	41.0	34.0	41.0
20	38.19101118567788	40.0	37.0	41.0	34.0	41.0
21	38.046988498112185	40.0	37.0	41.0	33.0	41.0
22	38.122268836118735	40.0	37.0	41.0	34.0	41.0
23	38.079722689852744	40.0	37.0	41.0	34.0	41.0
24	38.039846926548805	40.0	37.0	41.0	33.0	41.0
25	37.96272352214116	40.0	37.0	41.0	33.0	41.0
26	37.898803374099224	40.0	37.0	41.0	33.0	41.0
27	37.81962162199787	40.0	37.0	41.0	33.0	41.0
28	37.68729422737881	40.0	36.0	41.0	33.0	41.0
29	37.65171066592023	40.0	36.0	41.0	33.0	41.0
30	37.628398635325425	40.0	36.0	41.0	33.0	41.0
31	37.56947868118581	40.0	36.0	41.0	33.0	41.0
32	37.48661974562005	40.0	36.0	41.0	32.0	41.0
33	37.44462149770151	40.0	36.0	41.0	32.0	41.0
34	37.30851300786249	40.0	36.0	41.0	32.0	41.0
35	37.279735417799834	40.0	36.0	41.0	32.0	41.0
36	37.1341754328745	39.0	36.0	41.0	31.0	41.0
37	37.07639204463532	39.0	36.0	41.0	31.0	41.0
38	37.04061159774516	39.0	35.0	41.0	31.0	41.0
39	36.99779249667632	39.0	35.0	41.0	31.0	41.0
40	36.88212528874044	39.0	35.0	41.0	31.0	41.0
41	36.80465335734407	39.0	35.0	41.0	31.0	41.0
42	36.745851733337574	39.0	35.0	41.0	30.0	41.0
43	36.682526716259254	39.0	35.0	41.0	30.0	41.0
44	36.5170295954601	39.0	35.0	41.0	30.0	41.0
45	36.57656208205349	39.0	35.0	41.0	30.0	41.0
46	36.48466978435079	39.0	35.0	40.0	30.0	41.0
47	36.27688505371094	39.0	35.0	40.0	29.0	41.0
48	36.190977874253846	39.0	35.0	40.0	29.0	41.0
49	36.18465665865535	39.0	35.0	40.0	29.0	41.0
50	36.07809490474424	38.0	35.0	40.0	29.0	41.0
51	35.16131728286172	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	22.0
10	33.0
11	33.0
12	24.0
13	29.0
14	27.0
15	44.0
16	79.0
17	136.0
18	289.0
19	522.0
20	999.0
21	1644.0
22	2686.0
23	4163.0
24	6369.0
25	9514.0
26	13392.0
27	17278.0
28	20981.0
29	25464.0
30	31932.0
31	40958.0
32	53240.0
33	71687.0
34	110214.0
35	138829.0
36	165545.0
37	251920.0
38	444101.0
39	598951.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.114090135741566	4.227468301942702	55.51831084232162	16.140130719994115
2	19.81781136983536	3.9389018764772623	54.96365077297419	21.27963598071318
3	20.800498378678302	4.2227947588700365	52.786972946151835	22.18973391629983
4	18.95320590139222	4.467807740381699	52.23897516161012	24.340011196615958
5	17.841598709704364	6.029268312085286	51.37407138190703	24.755061596303328
6	20.66665606004409	6.064469040760256	55.11206062480299	18.156814274392662
7	85.40278483504878	1.9223674777086912	8.345158060221088	4.329689627021431
8	86.85461601871803	2.4270106924699277	6.519145119478631	4.199228169333404
9	81.31060068949677	5.573249832697102	8.589176670866227	4.526972806939914
10	33.84788711106427	39.51778979198755	15.024396889210182	11.609926207737995
11	22.533338769227402	25.821126602304357	32.827960913269976	18.817573715198264
12	22.7806885222754	22.86834231415954	34.80735556017386	19.5436136033912
13	23.142639517690355	22.580720541017303	34.55264746271357	19.723992478578765
14	18.89120687786441	26.272521257163202	33.33349906181109	21.502772803161303
15	16.70766789206303	28.20692062235684	35.12590226726501	19.95950921831512
16	20.001272794709152	26.513457318122114	33.533914509959125	19.951355377209616
17	19.863403274065515	26.686477848897393	30.746692971090656	22.703425905946435
18	19.522483222477554	26.208583210445667	32.98357995388108	21.2853536131957
19	21.343971775777323	27.10774306650054	31.683241171726856	19.865043985995282
20	23.617053858109244	27.94430727650769	30.49631038689976	17.942328478483304
21	23.858387667414764	26.640289322147325	30.909720074657365	18.591602935780546
22	21.144699854125797	24.48056551859921	31.436885789545382	22.937848837729614
23	20.400164667815496	25.94870438447946	31.22513451351897	22.425996434186075
24	20.676500331622684	25.78910786040226	31.393978686654844	22.140413121320208
25	20.39380069426974	27.14582747068843	29.134668640824295	23.325703194217535
26	19.758795458907127	28.475052726515198	28.993766289037758	22.772385525539917
27	20.652933742086052	28.02147045575	29.996539589384497	21.329056212779456
28	18.0856173203495	27.91432699488197	31.89146243117711	22.10859325359142
29	19.68660413399744	25.510485143602068	31.05887570463606	23.744035017764435
30	21.279337669453223	25.671821816695683	30.94129134966952	22.107549164181567
31	20.170415279104986	29.171858111232314	28.73950565846742	21.918220951195284
32	20.37575286304232	28.352794828475997	28.74512385386328	22.526328454618405
33	21.368731610353787	26.944268496043893	29.710508809628696	21.976491083973627
34	19.783455856396937	26.47393107617776	30.73898659687509	23.003626470550216
35	19.946880708310253	25.51391572309158	29.50119374222526	25.038009826372903
36	21.345861080423724	26.340188194630198	29.784291127924817	22.52965959702126
37	20.293518392380733	29.06590789540412	29.572887881701686	21.067685830513465
38	19.05502947812434	27.972547409116988	29.44834293066948	23.524080182089193
39	20.582035099302846	25.39613249395174	30.547172456722492	23.474659950022918
40	20.413340081796946	25.220824910183453	32.37949965246738	21.98633535555222
41	19.07317674643841	26.549652417663605	30.76409446125484	23.613076374643143
42	21.22768010293727	26.535432914272306	29.20695940282063	23.02992757996979
43	22.2099693634336	25.246032211649847	28.864945543279497	23.679052881637052
44	20.27731014725638	25.811878953245674	29.0590467364251	24.851764163072847
45	20.76847963677621	24.56523619788378	28.67337999584353	25.992904169496477
46	20.50362895647738	25.718706403052323	30.17821114669854	23.599453493771758
47	19.167393386041617	26.469804437081677	30.889285753350283	23.473516423526416
48	19.260367062061672	25.411495523839545	30.955709727234126	24.372427686864658
49	20.261002465045376	24.213427785307378	31.679313406804084	23.846256342843162
50	19.711214812943926	24.080082652106423	30.504066479658654	25.704636055290997
51	18.542232422257598	24.086198032935553	29.504624321714772	27.866945223092078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	390.0
1	1474.0
2	2558.0
3	5703.5
4	8849.0
5	6206.0
6	3563.0
7	3585.5
8	3608.0
9	3611.5
10	3615.0
11	3639.0
12	3663.0
13	3713.0
14	3763.0
15	3707.5
16	3652.0
17	4254.0
18	4856.0
19	6651.5
20	8447.0
21	9358.5
22	10270.0
23	12090.0
24	13910.0
25	15747.0
26	23299.0
27	29014.0
28	35171.0
29	41328.0
30	47460.5
31	53593.0
32	57035.5
33	60478.0
34	69923.0
35	79368.0
36	83632.0
37	87896.0
38	95597.0
39	103298.0
40	113221.5
41	123145.0
42	127304.0
43	131463.0
44	143680.0
45	155897.0
46	165831.0
47	175765.0
48	189656.5
49	203548.0
50	190596.5
51	177645.0
52	149888.5
53	122132.0
54	104066.5
55	86001.0
56	78969.5
57	71938.0
58	68515.0
59	65092.0
60	59706.0
61	54320.0
62	45519.5
63	36719.0
64	30789.5
65	24860.0
66	19866.5
67	14873.0
68	11998.5
69	9124.0
70	7527.0
71	5930.0
72	4952.5
73	3975.0
74	3222.0
75	1938.0
76	1407.0
77	956.5
78	506.0
79	454.0
80	402.0
81	318.0
82	234.0
83	142.0
84	50.0
85	73.5
86	97.0
87	59.5
88	22.0
89	13.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2011322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.86545156411726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.2372810353532	16.285135254162793
2	12.693901874721774	6.058914007016597
3	5.5547959322862575	3.977031398115999
4	2.9241049942991473	2.791403444393587
5	1.7409859827286347	2.077470832230866
6	1.1161270400427086	1.5982125488124495
7	0.7702481380163314	1.2867623741126186
8	0.5727243717619852	1.0934660603100117
9	0.4316089458033335	0.9270488151638249
>10	3.9780636725552716	23.24311099468306
>50	1.7416832087914285	29.15462419409251
>100	0.22875839596164776	8.352811393398822
>500	0.006970466377931985	1.0864384603215682
>1k	0.002112262538767268	1.1099799766991745
>5k	6.336787616301805E-4	0.9575902464861038
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6998	0.34793036619695905	RNA PCR Primer, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6161	0.30631594543290436	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	5837	0.2902071373952057	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4857	0.24148296493550014	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4570	0.22721374300087205	RNA PCR Primer, Index 38 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	2329	0.11579448740679016	RNA PCR Primer, Index 38 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9887417330492085E-4	0.0	0.0	0.3163590911848028	0.0
2	1.9887417330492085E-4	0.0	0.0	1.3042168285336708	0.0
3	1.9887417330492085E-4	0.0	0.0	1.8593740833143575	0.0
4	1.9887417330492085E-4	0.0	0.0	2.519238590340085	0.0
5	1.9887417330492085E-4	0.0	0.0	3.5523899206591487	0.0
6	1.9887417330492085E-4	0.0	0.0	4.1937591295675185	0.0
7	1.9887417330492085E-4	0.0	0.0	4.768455771875414	0.0
8	2.485927166311511E-4	0.0	0.0	5.662643773597663	0.0
9	2.485927166311511E-4	0.0	0.0	6.082516871987678	0.0
10	2.485927166311511E-4	0.0	0.0	6.934742423142589	0.0
11	2.485927166311511E-4	0.0	0.0	8.539358690453344	0.0
12	2.485927166311511E-4	0.0	0.0	9.991040718492613	0.0
13	2.485927166311511E-4	0.0	0.0	10.41906765798813	0.0
14	2.485927166311511E-4	0.0	0.0	10.576973751592236	0.0
15	2.485927166311511E-4	0.0	0.0	10.81025315687891	0.0
16	2.485927166311511E-4	0.0	0.0	11.406925395336998	0.0
17	2.485927166311511E-4	0.0	0.0	12.347003612549358	0.0
18	2.485927166311511E-4	0.0	0.0	13.383883833617888	0.0
19	2.485927166311511E-4	0.0	0.0	14.090086023023662	0.0
20	2.485927166311511E-4	0.0	0.0	14.806828543614598	0.0
21	2.485927166311511E-4	0.0	0.0	15.7657500887476	0.0
22	2.9831125995738125E-4	0.0	0.0	16.906243754107994	0.0
23	2.9831125995738125E-4	0.0	0.0	17.921247816112984	0.0
24	2.9831125995738125E-4	0.0	0.0	18.69352594959932	0.0
25	2.9831125995738125E-4	0.0	0.0	19.339966449926962	0.0
26	2.9831125995738125E-4	0.0	0.0	19.931467959879125	0.0
27	2.9831125995738125E-4	0.0	0.0	20.51894226782186	0.0
28	2.9831125995738125E-4	0.0	0.0	21.166973761535946	0.0
29	2.9831125995738125E-4	0.0	0.0	21.857166579990672	0.0
30	3.480298032836115E-4	0.0	0.0	22.65485088911671	0.0
31	3.480298032836115E-4	0.0	0.0	23.376117797150332	0.0
32	3.480298032836115E-4	0.0	0.0	24.022061112044714	0.0
33	3.977483466098417E-4	0.0	0.0	24.70012260592784	0.0
34	3.977483466098417E-4	0.0	0.0	25.337216020110155	0.0
35	3.977483466098417E-4	0.0	0.0	26.03591070947367	0.0
36	3.977483466098417E-4	0.0	0.0	26.704774272841444	0.0
37	3.977483466098417E-4	0.0	0.0	27.35449619702862	0.0
38	4.4746688993607187E-4	0.0	0.0	28.01212336960467	0.0
39	4.4746688993607187E-4	0.0	0.0	28.715541320584173	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	20	7.03455E-4	45.0	1
TCCGTCG	20	7.03455E-4	45.0	1
CGTAACA	20	7.03455E-4	45.0	22
CCGCGTA	20	7.03455E-4	45.0	26
TCGACGT	60	0.0	44.999996	26
CGAATAT	255	0.0	43.235294	14
TCGTTTG	570	0.0	42.236843	1
CAACCCG	75	0.0	42.0	23
CGTTCAT	265	0.0	41.603775	17
TGTTTCG	475	0.0	41.210526	1
CGGGTAC	230	0.0	41.086956	6
TCGTTCA	270	0.0	40.833336	16
CGCGACC	565	0.0	40.61947	10
TTCGTTG	875	0.0	39.85714	1
GCGCGAC	575	0.0	39.52174	9
GCCGATT	205	0.0	39.512196	9
TACGAAT	285	0.0	39.473686	12
GGGCGTA	40	3.4599543E-7	39.375	7
CGAGGTA	40	3.4599543E-7	39.375	13
CGCGTAC	40	3.4599543E-7	39.375	18
>>END_MODULE