##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793412
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02253936164313	33.0	31.0	34.0	30.0	34.0
2	32.204957827711205	34.0	31.0	34.0	30.0	34.0
3	32.27251667481712	34.0	31.0	34.0	30.0	34.0
4	35.825400926630806	37.0	35.0	37.0	35.0	37.0
5	35.7764893901277	37.0	35.0	37.0	35.0	37.0
6	35.40976440991565	37.0	35.0	37.0	33.0	37.0
7	35.77163314898186	37.0	35.0	37.0	35.0	37.0
8	35.86561206535823	37.0	35.0	37.0	35.0	37.0
9	37.60666337287563	39.0	37.0	39.0	35.0	39.0
10	37.11093731882049	39.0	37.0	39.0	33.0	39.0
11	37.00358956002682	39.0	37.0	39.0	33.0	39.0
12	36.622765977827406	39.0	35.0	39.0	33.0	39.0
13	36.38481016168144	39.0	35.0	39.0	32.0	39.0
14	37.1182802881731	39.0	36.0	41.0	31.0	41.0
15	37.23028136705772	39.0	35.0	41.0	31.0	41.0
16	37.39953895328026	39.0	35.0	41.0	32.0	41.0
17	37.449206717317104	39.0	35.0	41.0	33.0	41.0
18	37.43832334272736	39.0	36.0	41.0	33.0	41.0
19	37.490500521796996	39.0	36.0	41.0	33.0	41.0
20	37.39027768675039	39.0	35.0	41.0	32.0	41.0
21	37.209365373853686	39.0	35.0	41.0	32.0	41.0
22	37.38771029427334	39.0	35.0	41.0	32.0	41.0
23	37.38764979607064	39.0	35.0	41.0	33.0	41.0
24	37.337740039223	39.0	35.0	41.0	32.0	41.0
25	37.24390606645728	39.0	35.0	41.0	32.0	41.0
26	37.196335573447335	39.0	35.0	41.0	32.0	41.0
27	37.08993309907085	39.0	35.0	41.0	32.0	41.0
28	36.953333198892885	39.0	35.0	40.0	32.0	41.0
29	36.864417225854915	39.0	35.0	40.0	31.0	41.0
30	36.80721743558202	39.0	35.0	40.0	31.0	41.0
31	36.6943908083064	39.0	35.0	40.0	31.0	41.0
32	36.588392915660464	39.0	35.0	40.0	31.0	41.0
33	36.50292407979713	38.0	35.0	40.0	30.0	41.0
34	36.251300711358034	38.0	35.0	40.0	30.0	41.0
35	36.20938427954203	38.0	35.0	40.0	30.0	41.0
36	36.04896321205124	38.0	35.0	40.0	30.0	41.0
37	35.9552439337948	38.0	35.0	40.0	29.0	41.0
38	35.84387808603853	38.0	34.0	40.0	29.0	41.0
39	35.78677408458657	38.0	34.0	40.0	29.0	41.0
40	35.67602204151185	38.0	34.0	40.0	28.0	41.0
41	35.5979491109285	38.0	34.0	40.0	28.0	41.0
42	35.533476932539465	38.0	34.0	40.0	28.0	41.0
43	35.446968283817235	38.0	34.0	40.0	28.0	41.0
44	35.301349109920196	38.0	34.0	40.0	27.0	41.0
45	35.38529792844071	37.0	34.0	40.0	28.0	41.0
46	35.28546833171165	37.0	34.0	40.0	28.0	41.0
47	35.01226096908038	37.0	34.0	40.0	26.0	41.0
48	34.87762095859402	37.0	34.0	40.0	26.0	41.0
49	34.88865935982818	36.0	34.0	40.0	27.0	41.0
50	34.72161247876261	36.0	33.0	40.0	26.0	41.0
51	33.66197889621029	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	34.0
11	30.0
12	21.0
13	12.0
14	19.0
15	30.0
16	52.0
17	127.0
18	210.0
19	469.0
20	745.0
21	1140.0
22	1738.0
23	2612.0
24	3817.0
25	5588.0
26	7500.0
27	9419.0
28	11335.0
29	14048.0
30	17168.0
31	22039.0
32	29423.0
33	41199.0
34	66925.0
35	69193.0
36	70687.0
37	101430.0
38	155178.0
39	161164.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.10940091654777	4.7909535020897085	54.9026231012387	15.19702248012382
2	21.426446789309967	4.303942970360922	52.51609504267643	21.75351519765267
3	22.33291152642007	4.865567952085423	50.8875590487666	21.913961472727912
4	20.328152334474396	5.033324426653492	49.47820300171916	25.160320237152956
5	18.01107117109396	6.434361970829783	49.4396353974984	26.114931460577857
6	21.699066815223365	6.6871940429436405	53.32387208663343	18.28986705519957
7	81.81033309302103	2.6663322460461902	10.139120658623767	5.384214002309014
8	82.50618846198444	3.1283872691615455	8.53049865643575	5.834925612418265
9	76.68197607296084	5.655447611077221	11.417775380256412	6.244800935705535
10	42.367143426114055	24.33300731524101	18.632186052139367	14.667663206505575
11	31.291561004875145	23.28122085373047	25.14696021739021	20.280257924004175
12	29.30154824983741	19.321361411221407	31.201695966282333	20.175394372658843
13	28.302823753610983	21.471190251722938	31.314121792965068	18.911864201701007
14	21.195293239829997	24.230538484419192	34.039313748720716	20.534854527030095
15	18.09967583046387	24.20646524126179	37.752895091075004	19.940963837199334
16	22.793580132390233	23.181020705509876	32.20571405524495	21.81968510685495
17	24.951853513685197	21.79586393954213	28.905411060079757	24.346871486692915
18	24.019551002505636	22.33669266408877	32.32570215726508	21.318054176140517
19	25.872560536014078	24.380019460255202	29.35410606343237	20.39331394029836
20	24.514123809571824	28.030707879386753	29.135052154492243	18.320116156549183
21	23.98589887725419	25.299214027516598	32.600590865779694	18.11429622944952
22	22.139443315704828	22.322828492636866	31.9197340095688	23.617994182089507
23	21.521101268949803	23.712774699651632	31.218459009946915	23.547665021451657
24	22.499281583842947	24.262804192525447	29.68760240581186	23.550311817819747
25	23.98728529439938	23.834149218816957	27.078365338563064	25.100200148220598
26	21.269403538136554	24.510090596058543	28.01243238065469	26.208073485150212
27	20.796761329548836	26.059222698925655	30.97381940278191	22.170196568743602
28	18.392840037710545	24.99836150701023	30.794719515207735	25.81407894007149
29	22.153685600923605	25.827438959834236	29.26499725237329	22.753878186868864
30	24.709230513276832	24.846107696883838	28.511290477078745	21.93337131276058
31	23.87725418824016	26.055189485412374	27.288218479176013	22.77933784717146
32	26.705167050662205	25.38365943545094	26.60610124374222	21.30507227014464
33	26.119468825780302	23.87775833992932	27.593230251117955	22.409542583172424
34	20.78806471291082	24.303262365580554	31.031418733268467	23.87725418824016
35	22.947346397584106	25.999480723760165	27.62423558000131	23.42893729865442
36	27.49113953406301	24.558363120295635	27.516599194365597	20.433898151275756
37	23.36617041335397	27.96403381849531	27.9185341285486	20.751261639602124
38	22.403996914591662	28.358028363574032	27.571551728484067	21.66642299335024
39	20.689377019757703	24.597939027894714	30.15255630113989	24.560127651207694
40	24.09340922496761	22.862018724193735	29.323983000005043	23.720589050833617
41	22.39744294263258	21.715451745121072	28.130025762151316	27.757079550095032
42	23.7512162659501	21.96677136216745	29.824731665263442	24.45728070661901
43	22.239769501847718	22.038739015795073	31.033939491714268	24.687551990642945
44	21.719863072401225	23.27617933683887	29.90867292150862	25.095284669251285
45	22.7483325182881	22.31299753469824	27.054922285017113	27.883747661996544
46	23.306554476110772	23.272146123325584	29.10669362197698	24.31460577858666
47	19.626373183163352	24.861736399247807	31.673581947336316	23.838308470252528
48	20.782645082252348	25.2370773318276	30.880551340287266	23.099726245632787
49	22.985031736348834	21.94698340836791	32.20180687965395	22.866177975629306
50	21.611722535076353	22.77820350587085	29.152319349845985	26.45775460920682
51	20.940822674726373	22.836306988046566	26.726845573296092	29.496024763930972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	289.0
1	709.5
2	1130.0
3	2407.5
4	3685.0
5	2571.0
6	1457.0
7	1492.0
8	1527.0
9	1625.0
10	1723.0
11	1841.5
12	1960.0
13	1812.0
14	1664.0
15	1671.5
16	1679.0
17	1677.0
18	1675.0
19	1857.0
20	2039.0
21	2516.5
22	2994.0
23	2977.0
24	2960.0
25	3322.0
26	5327.5
27	6971.0
28	7365.5
29	7760.0
30	8961.0
31	10162.0
32	11839.5
33	13517.0
34	14946.5
35	16376.0
36	18497.5
37	20619.0
38	23822.5
39	27026.0
40	30294.5
41	33563.0
42	38259.0
43	42955.0
44	48510.5
45	54066.0
46	62757.0
47	71448.0
48	84014.5
49	96581.0
50	91537.5
51	86494.0
52	74557.5
53	62621.0
54	55864.0
55	49107.0
56	44585.5
57	40064.0
58	36642.0
59	33220.0
60	29728.5
61	26237.0
62	24503.5
63	22770.0
64	19356.5
65	15943.0
66	13330.5
67	10718.0
68	8274.0
69	5830.0
70	4941.0
71	4052.0
72	3412.5
73	2773.0
74	2437.5
75	1563.5
76	1025.0
77	807.0
78	589.0
79	353.0
80	117.0
81	90.5
82	64.0
83	51.0
84	38.0
85	79.5
86	121.0
87	67.5
88	14.0
89	8.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	793412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.458757289302234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0448965643335	18.95887056825011
2	12.266947770824649	6.736702830392422
3	5.375787643723532	4.428373444435032
4	2.9048000756085584	3.190488010003287
5	1.794454830940542	2.4636749834706104
6	1.1492655376072456	1.8934442074870081
7	0.7812807227963684	1.5017098419453268
8	0.6100498053721765	1.3400967632080527
9	0.446908097016059	1.104438686992911
>10	4.334563922631766	27.795053070652248
>50	1.1441985009054922	21.153780548803052
>100	0.14036119260805668	6.399150902991596
>500	0.0037059060754602432	0.7839303294731892
>1k	0.002779429556595182	2.250285811895212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	4018	0.5064203717614556	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	4003	0.5045298029271047	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3734	0.47062560183107893	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	2420	0.3050117719419419	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2246	0.28308117346347167	No Hit
TCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	1270	0.16006816130837445	No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	894	0.11267790252731241	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	893	0.11255186460502235	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	893	0.11255186460502235	No Hit
TGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	874	0.11015714408151124	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.781137668701759E-4	0.0	0.0	0.5568355406774791	0.0
2	3.781137668701759E-4	0.0	0.0	2.0219003493771206	0.0
3	3.781137668701759E-4	0.0	0.0	2.8158132218822	0.0
4	3.781137668701759E-4	0.0	0.0	3.7400493060352	0.0
5	3.781137668701759E-4	0.0	0.0	5.147262708403704	0.0
6	3.781137668701759E-4	0.0	0.0	6.028393823133505	0.0
7	3.781137668701759E-4	0.0	0.0	6.825079529928965	0.0
8	3.781137668701759E-4	0.0	0.0	8.08293799438375	0.0
9	3.781137668701759E-4	0.0	0.0	8.696868713858626	0.0
10	5.041516891602345E-4	0.0	0.0	9.86725686024411	0.0
11	5.041516891602345E-4	0.0	0.0	12.114513014675856	0.0
12	5.041516891602345E-4	0.0	0.0	14.140824691333128	0.0
13	5.041516891602345E-4	0.0	0.0	14.781601488255786	0.0
14	5.041516891602345E-4	0.0	0.0	15.015527872026135	0.0
15	5.041516891602345E-4	0.0	0.0	15.351418934929141	0.0
16	5.041516891602345E-4	0.0	0.0	16.19284810413757	0.0
17	5.041516891602345E-4	0.0	0.0	17.383528356011755	0.0
18	5.041516891602345E-4	0.0	0.0	18.89787399232681	0.0
19	5.041516891602345E-4	0.0	0.0	19.782156055113862	0.0
20	5.041516891602345E-4	0.0	0.0	20.754916739348534	0.0
21	5.041516891602345E-4	0.0	0.0	22.000675563263474	0.0
22	5.041516891602345E-4	0.0	0.0	23.247946842245895	0.0
23	5.041516891602345E-4	0.0	0.0	24.348005827993525	0.0
24	6.301896114502931E-4	0.0	0.0	25.183007063165164	0.0
25	6.301896114502931E-4	0.0	0.0	25.932932700791014	0.0
26	6.301896114502931E-4	0.0	0.0	26.584674796952907	0.0
27	7.562275337403518E-4	0.0	0.0	27.309392850120744	0.0
28	7.562275337403518E-4	0.0	0.0	27.961260984204927	0.0
29	7.562275337403518E-4	0.0	0.0	28.75920707022329	0.0
30	7.562275337403518E-4	0.0	0.0	29.652815939259803	0.0
31	7.562275337403518E-4	0.0	0.0	30.382575509319246	0.0
32	7.562275337403518E-4	0.0	0.0	31.092295049734563	0.0
33	7.562275337403518E-4	0.0	0.0	31.77037907165508	0.0
34	7.562275337403518E-4	0.0	0.0	32.54614248335039	0.0
35	7.562275337403518E-4	0.0	0.0	33.337282521565086	0.0
36	7.562275337403518E-4	0.0	0.0	33.999359727354765	0.0
37	7.562275337403518E-4	0.0	0.0	34.7305057145594	0.0
38	7.562275337403518E-4	0.0	0.0	35.60974626045485	0.0
39	7.562275337403518E-4	0.0	0.0	37.18837123713783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.0319645E-4	45.0	40
AACCGTT	20	7.0319645E-4	45.0	11
GTCGCAA	20	7.0319645E-4	45.0	31
CAAACGC	25	3.8897102E-5	45.0	13
CTCGTTA	20	7.0319645E-4	45.0	24
GATCGGT	20	7.0319645E-4	45.0	34
GCGAATT	20	7.0319645E-4	45.0	43
CGTGATA	20	7.0319645E-4	45.0	23
CGGTTGA	35	1.2112832E-7	45.0	31
GCGCCTA	25	3.8897102E-5	45.0	29
CACGAAC	25	3.8897102E-5	45.0	12
CCGCTCG	20	7.0319645E-4	45.0	15
TAGCCGT	25	3.8897102E-5	45.0	44
CCGTCGA	25	3.8897102E-5	45.0	13
CGTTAAT	20	7.0319645E-4	45.0	26
GTATGCG	25	3.8897102E-5	45.0	9
TACGACG	20	7.0319645E-4	45.0	35
AGCGCTA	20	7.0319645E-4	45.0	10
TACTCCG	20	7.0319645E-4	45.0	45
GGACCGT	20	7.0319645E-4	45.0	8
>>END_MODULE