SRR2934345.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547122
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3285	0.600414532773312	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3143	0.5744605407934611	Illumina PCR Primer Index 2 (95% over 24bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3053	0.558010827566795	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2869	0.5243803027478332	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2743	0.5013507042305008	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1434	0.2620987640782129	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	944	0.17253921428858646	Illumina PCR Primer Index 2 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	766	0.1400053370180691	Illumina PCR Primer Index 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	726	0.13269435336177307	Illumina PCR Primer Index 2 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	626	0.11441689422103296	Illumina PCR Primer Index 2 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	25	3.888116E-5	45.000004	24
TCCGATA	25	3.888116E-5	45.000004	14
CGCATGG	25	3.888116E-5	45.000004	2
GTGATAG	25	3.888116E-5	45.000004	18
CGTCTAT	25	3.888116E-5	45.000004	21
CGCGAGG	25	3.888116E-5	45.000004	18
ACTTAGT	25	3.888116E-5	45.000004	42
AACGGGG	25	3.888116E-5	45.000004	4
CCCGTAG	25	3.888116E-5	45.000004	4
CTTATCG	25	3.888116E-5	45.000004	1
AGCGCTA	25	3.888116E-5	45.000004	19
CCGACTC	25	3.888116E-5	45.000004	27
GGCATAA	25	3.888116E-5	45.000004	8
CGCAATC	25	3.888116E-5	45.000004	18
CAATCGG	25	3.888116E-5	45.000004	20
TGTCGGC	25	3.888116E-5	45.000004	27
CGATAAC	25	3.888116E-5	45.000004	16
TAGTACT	25	3.888116E-5	45.000004	22
CTTACGG	25	3.888116E-5	45.000004	2
TAATAAG	20	7.0300436E-4	45.0	39