##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547122
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01139416802834	33.0	31.0	34.0	30.0	34.0
2	32.196745881174586	34.0	31.0	34.0	30.0	34.0
3	32.27987176534667	34.0	31.0	34.0	30.0	34.0
4	35.81787243064618	37.0	35.0	37.0	35.0	37.0
5	35.77259185337091	37.0	35.0	37.0	35.0	37.0
6	35.361054755612095	37.0	35.0	37.0	33.0	37.0
7	35.74033762122525	37.0	35.0	37.0	35.0	37.0
8	35.823308146994634	37.0	35.0	37.0	35.0	37.0
9	37.57189255778419	39.0	37.0	39.0	35.0	39.0
10	37.11202071932768	39.0	37.0	39.0	33.0	39.0
11	37.02324892802702	39.0	37.0	39.0	33.0	39.0
12	36.79173749182084	39.0	35.0	39.0	33.0	39.0
13	36.60438622464459	39.0	35.0	39.0	33.0	39.0
14	37.6535672117005	39.0	36.0	41.0	33.0	41.0
15	37.73046231005151	39.0	36.0	41.0	33.0	41.0
16	37.799543794619844	40.0	36.0	41.0	33.0	41.0
17	37.751969396222414	39.0	36.0	41.0	33.0	41.0
18	37.72603916493944	39.0	36.0	41.0	33.0	41.0
19	37.778502783657025	39.0	36.0	41.0	33.0	41.0
20	37.686154093602525	39.0	36.0	41.0	33.0	41.0
21	37.52340976966746	39.0	36.0	41.0	32.0	41.0
22	37.58924700523832	39.0	36.0	41.0	33.0	41.0
23	37.5459988814195	39.0	35.0	41.0	33.0	41.0
24	37.52995675553167	39.0	35.0	41.0	33.0	41.0
25	37.45753963467015	39.0	35.0	41.0	33.0	41.0
26	37.41696184763179	39.0	35.0	41.0	33.0	41.0
27	37.324773999217726	39.0	35.0	41.0	32.0	41.0
28	37.19364602410431	39.0	35.0	41.0	32.0	41.0
29	37.11590102390326	39.0	35.0	41.0	32.0	41.0
30	37.0656471499958	39.0	35.0	41.0	32.0	41.0
31	36.94117399775553	39.0	35.0	40.0	31.0	41.0
32	36.834066259444874	39.0	35.0	40.0	31.0	41.0
33	36.792230983217635	39.0	35.0	40.0	31.0	41.0
34	36.64941457298372	39.0	35.0	40.0	30.0	41.0
35	36.63695848457931	39.0	35.0	40.0	30.0	41.0
36	36.47804694382606	39.0	35.0	40.0	30.0	41.0
37	36.41983871970054	39.0	35.0	40.0	30.0	41.0
38	36.32309978396043	38.0	35.0	40.0	30.0	41.0
39	36.23925925113594	38.0	35.0	40.0	30.0	41.0
40	36.06994966387752	38.0	35.0	40.0	30.0	41.0
41	36.0237917685635	38.0	35.0	40.0	30.0	41.0
42	36.022581800768386	38.0	35.0	40.0	30.0	41.0
43	36.01126439806844	38.0	35.0	40.0	30.0	41.0
44	35.84530506907052	38.0	35.0	40.0	29.0	41.0
45	35.85972232884073	38.0	35.0	40.0	29.0	41.0
46	35.681725465252725	38.0	34.0	40.0	29.0	41.0
47	35.45604636625835	38.0	34.0	40.0	28.0	41.0
48	35.403878842378845	38.0	34.0	40.0	28.0	41.0
49	35.457448247374444	38.0	34.0	40.0	28.0	41.0
50	35.300843321964756	37.0	34.0	40.0	28.0	41.0
51	34.38547709651595	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	13.0
11	13.0
12	11.0
13	10.0
14	11.0
15	20.0
16	27.0
17	52.0
18	123.0
19	221.0
20	376.0
21	614.0
22	956.0
23	1450.0
24	2193.0
25	3184.0
26	4407.0
27	5534.0
28	6642.0
29	8359.0
30	10529.0
31	13443.0
32	17403.0
33	23822.0
34	40149.0
35	50106.0
36	49797.0
37	73432.0
38	116045.0
39	118127.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.320860795215694	4.626938781478354	52.289982855743325	17.762217567562626
2	20.701781321167857	5.503160172685434	49.735525166233494	24.05953333991322
3	21.760960078373746	4.659107109566056	51.42948007939728	22.150452732662917
4	21.344051235373463	5.35712327415092	47.77709541930319	25.521730071172428
5	18.070375528675505	7.54840785053425	47.80378050964867	26.577436111141573
6	20.939022740814664	7.684209371949949	51.85516210278512	19.52160578445027
7	80.60926082299743	3.751631263228311	10.095920105570604	5.543187808203655
8	79.86189551873257	4.235252832092294	9.50829979419581	6.394551854979329
9	73.83051677687973	6.2351724112720746	12.351358563537932	7.5829522483102485
10	38.00194472165258	27.326263612137698	19.653934588629227	15.017857077580505
11	27.86398646005827	25.904825614762338	26.12141350558011	20.109774419599287
12	26.502498528664535	19.855717737542996	32.574453229809805	21.06733050398266
13	24.31486944410936	24.801049857253044	30.83297692288009	20.051103775757507
14	19.23318747920939	26.497380840105134	32.07182310343945	22.197608577246026
15	16.877040221376586	24.697782213107864	35.83551748970065	22.5896600758149
16	20.265315596886982	23.825764637503152	32.09960484133338	23.809314924276485
17	20.61551171402356	23.41525290520213	29.782388571470346	26.186846809303958
18	21.719470246124263	22.399757275342612	31.7395388962608	24.141233582272324
19	24.37500228468239	24.20630133681336	29.090952292176155	22.327744086328096
20	23.379794634469096	25.717664433161158	30.90078629629223	20.00175463607751
21	24.90267253007556	25.08325382638607	30.511110867411656	19.502962776126715
22	20.88108319533852	23.39697544606139	29.049645234518078	26.672296124082013
23	20.78366433811837	25.505097583354353	28.600933612612913	25.110304465914368
24	22.694024367508526	23.166679460888066	29.302970818208735	24.836325353394674
25	21.187961734311543	24.517749240571575	28.08075712546745	26.21353189964944
26	18.863251706200813	25.57564857563761	27.768760897935014	27.79233882022657
27	19.34431443078509	24.892802702139562	29.805600944579087	25.95728192249626
28	17.879741629837586	23.710433870325083	33.1719433691206	25.237881130716733
29	21.128925541286954	22.487854628400978	30.3961456494164	25.987074180895668
30	22.2142410650641	23.770018387123894	28.9414426764049	25.074297871407108
31	21.951045653437443	28.900135618746823	25.61074129718783	23.538077430627904
32	22.226669737279803	27.131243123105996	25.310625418096876	25.33146172151732
33	21.048504721067694	25.27882263919199	26.168021026388992	27.504651613351317
34	18.784841406487036	25.42559063609213	29.188919473170515	26.60064848425031
35	19.627249498283746	23.70166068993753	27.436111141573544	29.23497867020518
36	20.638541312540895	23.999948823114405	28.03652567434686	27.324984189997842
37	19.123705498956355	25.545856317238204	30.18887926276041	25.141558921045032
38	20.949440892524883	27.064347622650892	28.424190582721952	23.562020902102272
39	21.643436016098786	23.982402462339294	29.196413231418223	25.177748290143697
40	21.53962004817938	24.61772694207142	29.230043756237183	24.612609253512012
41	18.10035056166632	25.843778901232266	26.322100006945437	29.73377053015598
42	20.10374285808284	23.78719919871619	26.404531347670172	29.704526595530794
43	20.941581585094365	20.928421814513033	28.786998146665642	29.342998453726953
44	20.71622051388904	21.94848680915774	29.250331735883407	28.084960941069813
45	22.870950171990888	21.62625520450649	27.316576558793106	28.186218064709518
46	21.505075650403384	24.652454114438825	29.05823564031423	24.784234594843564
47	17.871333998632846	25.293079057321766	30.978282723048974	25.85730422099641
48	18.5468688884746	23.38107405660895	30.571974806350322	27.500082248566134
49	20.925497421050515	20.341167052321055	32.312537240322996	26.42079828630543
50	20.872310014950962	21.661165151465305	30.115769426197446	27.350755407386284
51	19.019706756445544	22.017758379301146	27.236338513165254	31.72619635108806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	454.0
2	730.0
3	1697.0
4	2664.0
5	1816.5
6	969.0
7	1025.5
8	1082.0
9	1099.0
10	1116.0
11	1291.5
12	1467.0
13	1416.5
14	1366.0
15	1383.0
16	1400.0
17	1341.5
18	1283.0
19	1176.0
20	1069.0
21	1260.0
22	1451.0
23	1747.0
24	2043.0
25	2110.5
26	2736.0
27	3294.0
28	3951.0
29	4608.0
30	5671.5
31	6735.0
32	7852.0
33	8969.0
34	9867.5
35	10766.0
36	11756.0
37	12746.0
38	14715.0
39	16684.0
40	19174.0
41	21664.0
42	25496.5
43	29329.0
44	34946.5
45	40564.0
46	46679.0
47	52794.0
48	62625.0
49	72456.0
50	67319.5
51	62183.0
52	52483.5
53	42784.0
54	37191.5
55	31599.0
56	28708.5
57	25818.0
58	23598.0
59	21378.0
60	19712.5
61	18047.0
62	16191.0
63	14335.0
64	12692.0
65	11049.0
66	9326.0
67	7603.0
68	6117.0
69	4631.0
70	3967.5
71	3304.0
72	2657.5
73	2011.0
74	1797.0
75	1064.5
76	546.0
77	366.5
78	187.0
79	238.0
80	289.0
81	192.0
82	95.0
83	59.0
84	23.0
85	17.5
86	12.0
87	9.5
88	7.0
89	20.0
90	33.0
91	16.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	547122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.689982014325533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65858395660594	19.985035208584094
2	12.489772666983132	7.166627063575214
3	5.359435135813696	4.612862929603177
4	2.875290555431271	3.299681372851329
5	1.624460297327077	2.3302868356649866
6	1.0410983779522258	1.7921456243155707
7	0.7728873498829398	1.5521886917068886
8	0.5257634895622153	1.206731604746319
9	0.428091481208934	1.1053743206733262
>10	4.066854469884496	28.797296822119506
>50	1.0389679816445634	19.466743213930403
>100	0.1123729275573539	5.076198269248094
>500	0.0025685240584538034	0.5641039087042775
>1k	0.0038527860876807047	3.044724134276811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3285	0.600414532773312	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3143	0.5744605407934611	Illumina PCR Primer Index 2 (95% over 24bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3053	0.558010827566795	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2869	0.5243803027478332	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2743	0.5013507042305008	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1434	0.2620987640782129	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	944	0.17253921428858646	Illumina PCR Primer Index 2 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	766	0.1400053370180691	Illumina PCR Primer Index 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	726	0.13269435336177307	Illumina PCR Primer Index 2 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	626	0.11441689422103296	Illumina PCR Primer Index 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.827745914074009E-4	0.0	0.0	0.6590851766150877	0.0
2	1.827745914074009E-4	0.0	0.0	2.489207160377393	0.0
3	1.827745914074009E-4	0.0	0.0	3.3979624288549903	0.0
4	1.827745914074009E-4	0.0	0.0	4.484009050997766	0.0
5	1.827745914074009E-4	0.0	0.0	6.097360369350894	0.0
6	1.827745914074009E-4	0.0	0.0	7.131316232942561	0.0
7	1.827745914074009E-4	0.0	0.0	8.061821677797639	0.0
8	1.827745914074009E-4	0.0	0.0	9.516890199991957	0.0
9	1.827745914074009E-4	0.0	0.0	10.201015495629859	0.0
10	1.827745914074009E-4	0.0	0.0	11.478609889567592	0.0
11	1.827745914074009E-4	0.0	0.0	13.775355405192991	0.0
12	1.827745914074009E-4	0.0	0.0	15.79446631647055	0.0
13	1.827745914074009E-4	0.0	0.0	16.5118565877446	0.0
14	1.827745914074009E-4	0.0	0.0	16.753301822993773	0.0
15	1.827745914074009E-4	0.0	0.0	17.097649153205317	0.0
16	1.827745914074009E-4	0.0	0.0	18.087373565676394	0.0
17	1.827745914074009E-4	0.0	0.0	19.38726645976583	0.0
18	1.827745914074009E-4	0.0	0.0	21.00975650768933	0.0
19	3.655491828148018E-4	0.0	0.0	21.910835243327814	0.0
20	3.655491828148018E-4	0.0	0.0	22.930351914198297	0.0
21	3.655491828148018E-4	0.0	0.0	24.109248028776033	0.0
22	5.483237742222027E-4	0.0	0.0	25.2618246021911	0.0
23	5.483237742222027E-4	0.0	0.0	26.31405792492351	0.0
24	5.483237742222027E-4	0.0	0.0	27.113148438556667	0.0
25	5.483237742222027E-4	0.0	0.0	27.827797090959603	0.0
26	5.483237742222027E-4	0.0	0.0	28.496386546327876	0.0
27	5.483237742222027E-4	0.0	0.0	29.151450681932	0.0
28	5.483237742222027E-4	0.0	0.0	29.891505002540566	0.0
29	5.483237742222027E-4	0.0	0.0	30.623334466535802	0.0
30	5.483237742222027E-4	0.0	0.0	31.393546594726587	0.0
31	5.483237742222027E-4	0.0	0.0	32.10362588234434	0.0
32	5.483237742222027E-4	0.0	0.0	32.75558284989454	0.0
33	5.483237742222027E-4	0.0	0.0	33.50897971567584	0.0
34	5.483237742222027E-4	0.0	0.0	34.18159021205508	0.0
35	5.483237742222027E-4	0.0	0.0	34.86260833963906	0.0
36	5.483237742222027E-4	0.0	0.0	35.526811204813555	0.0
37	5.483237742222027E-4	0.0	0.0	36.17438158216997	0.0
38	5.483237742222027E-4	0.0	0.0	36.813361553730246	0.0
39	5.483237742222027E-4	0.0	0.0	37.47628499676489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	25	3.888116E-5	45.000004	24
TCCGATA	25	3.888116E-5	45.000004	14
CGCATGG	25	3.888116E-5	45.000004	2
GTGATAG	25	3.888116E-5	45.000004	18
CGTCTAT	25	3.888116E-5	45.000004	21
CGCGAGG	25	3.888116E-5	45.000004	18
ACTTAGT	25	3.888116E-5	45.000004	42
AACGGGG	25	3.888116E-5	45.000004	4
CCCGTAG	25	3.888116E-5	45.000004	4
CTTATCG	25	3.888116E-5	45.000004	1
AGCGCTA	25	3.888116E-5	45.000004	19
CCGACTC	25	3.888116E-5	45.000004	27
GGCATAA	25	3.888116E-5	45.000004	8
CGCAATC	25	3.888116E-5	45.000004	18
CAATCGG	25	3.888116E-5	45.000004	20
TGTCGGC	25	3.888116E-5	45.000004	27
CGATAAC	25	3.888116E-5	45.000004	16
TAGTACT	25	3.888116E-5	45.000004	22
CTTACGG	25	3.888116E-5	45.000004	2
TAATAAG	20	7.0300436E-4	45.0	39
>>END_MODULE