##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613069
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.276945988135104	33.0	31.0	34.0	31.0	34.0
2	32.468754740494134	34.0	31.0	34.0	31.0	34.0
3	32.529840849888025	34.0	31.0	34.0	31.0	34.0
4	35.99732330292349	37.0	35.0	37.0	35.0	37.0
5	35.97351195379313	37.0	35.0	37.0	35.0	37.0
6	35.58366839621641	37.0	35.0	37.0	33.0	37.0
7	35.92366764589304	37.0	35.0	37.0	35.0	37.0
8	36.00440244083455	37.0	35.0	37.0	35.0	37.0
9	37.796820586263536	39.0	38.0	39.0	35.0	39.0
10	37.37063364808855	39.0	37.0	39.0	35.0	39.0
11	37.283017082905836	39.0	37.0	39.0	34.0	39.0
12	36.999047415543764	39.0	37.0	39.0	33.0	39.0
13	36.84383650127474	39.0	35.0	39.0	33.0	39.0
14	37.99084931712417	40.0	37.0	41.0	33.0	41.0
15	38.07599633972685	40.0	37.0	41.0	33.0	41.0
16	38.1298059435398	40.0	37.0	41.0	33.0	41.0
17	38.06897755391318	40.0	37.0	41.0	33.0	41.0
18	38.050963268408616	40.0	37.0	41.0	33.0	41.0
19	38.04347797719343	40.0	37.0	41.0	34.0	41.0
20	37.97566668678403	40.0	36.0	41.0	34.0	41.0
21	37.854571018922826	40.0	36.0	41.0	33.0	41.0
22	37.90267653396273	40.0	36.0	41.0	33.0	41.0
23	37.86249671733524	40.0	36.0	41.0	33.0	41.0
24	37.81752298680899	40.0	36.0	41.0	33.0	41.0
25	37.76588442736462	40.0	36.0	41.0	33.0	41.0
26	37.685081124636866	40.0	35.0	41.0	33.0	41.0
27	37.60319474643148	39.0	35.0	41.0	33.0	41.0
28	37.49174399618966	39.0	35.0	41.0	33.0	41.0
29	37.41362554622726	39.0	35.0	41.0	33.0	41.0
30	37.414268214507665	39.0	35.0	41.0	33.0	41.0
31	37.33252211415029	39.0	35.0	41.0	33.0	41.0
32	37.21688423325922	39.0	35.0	41.0	32.0	41.0
33	37.12133870738857	39.0	35.0	41.0	32.0	41.0
34	36.964630408648944	39.0	35.0	41.0	31.0	41.0
35	36.909825810797805	39.0	35.0	41.0	31.0	41.0
36	36.751045967093425	39.0	35.0	41.0	31.0	41.0
37	36.71619181527691	39.0	35.0	41.0	31.0	41.0
38	36.648494704511236	39.0	35.0	41.0	31.0	41.0
39	36.60141680626487	39.0	35.0	41.0	31.0	41.0
40	36.45513800241082	39.0	35.0	41.0	30.0	41.0
41	36.34378838271059	39.0	35.0	40.0	30.0	41.0
42	36.25317052403563	38.0	35.0	40.0	30.0	41.0
43	36.19317238353268	38.0	35.0	40.0	30.0	41.0
44	36.073866073802456	38.0	35.0	40.0	30.0	41.0
45	36.11716136356593	38.0	35.0	40.0	30.0	41.0
46	36.01694589026684	38.0	35.0	40.0	30.0	41.0
47	35.798309815045286	38.0	35.0	40.0	29.0	41.0
48	35.70447209041723	38.0	35.0	40.0	29.0	41.0
49	35.67264043688394	38.0	35.0	40.0	29.0	41.0
50	35.55863858717371	37.0	34.0	40.0	29.0	41.0
51	34.623841688292835	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	19.0
10	31.0
11	26.0
12	16.0
13	14.0
14	8.0
15	27.0
16	37.0
17	47.0
18	115.0
19	208.0
20	400.0
21	639.0
22	1021.0
23	1485.0
24	2225.0
25	3322.0
26	4608.0
27	5585.0
28	6495.0
29	7898.0
30	9835.0
31	12302.0
32	16929.0
33	23958.0
34	43910.0
35	53295.0
36	50256.0
37	75554.0
38	127525.0
39	165200.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.56308996214129	4.796523719189846	53.649099856622996	17.99128646204587
2	21.23203097856848	4.813487551972127	52.9025281004259	21.0519533690335
3	22.468922747684193	4.981331628250653	50.6241548667442	21.925590757320954
4	19.320989970133866	5.089476062237693	49.92602790224265	25.66350606538579
5	18.644883365493932	6.077619321805539	49.605346217146845	25.67215109555368
6	21.91939243380435	6.995623657369725	52.59195946948875	18.493024439337173
7	83.30954590755691	2.5737722833808268	9.345277611492344	4.7714041975699315
8	84.67285085365594	3.438275300170128	7.508779599033715	4.380094247140208
9	78.66667536606809	5.870464825329612	9.787805287822415	5.67505452077988
10	39.88898476354211	29.067201244884345	16.393423904976437	14.650390086597104
11	32.72763750899165	25.37234797388222	23.52736804503245	18.372646472093678
12	31.589266461034565	21.079193369751202	28.59449751985502	18.737042649359207
13	25.127677308753178	23.12839174709535	29.854225217716113	21.88970572643536
14	20.48545922237138	26.181229192798856	30.652993382474076	22.68031820235569
15	19.294891765853436	25.803783913393108	34.20185982328253	20.69946449747092
16	22.218053759038543	22.978000844929365	33.368837765406504	21.43510763062559
17	21.800808718105138	22.848325392410967	30.115533488073936	25.235332401409956
18	22.758612815196987	24.427103637600336	30.07100342702045	22.74328012018223
19	24.8120521507367	26.38822057549803	28.233363618124553	20.566363655640718
20	28.527294643832914	26.17030056975642	27.202647662824248	18.099757123586414
21	26.717057949431467	25.210702221120297	28.212648168476957	19.859591660971276
22	23.907260031089486	24.3192854311668	27.789531031580456	23.983923506163254
23	23.744146254336787	25.33303755368482	27.715477376934732	23.207338815043656
24	23.34696420794397	24.161880636600447	29.12021322232897	23.370941933126613
25	23.079131386516035	24.54568735329955	27.261042394901715	25.114138865282698
26	21.444242002123744	27.222873771141586	28.331558111729677	23.001326115005
27	20.957836719847194	26.660946810228538	29.679856590367482	22.701359879556787
28	20.214853466738656	28.131417507654117	29.864990726981794	21.78873829862544
29	21.709954344453884	25.941288827195635	29.836935157380328	22.511821670970154
30	22.497630772392668	24.336249263949085	28.845203394723924	24.32091656893433
31	24.388445672509945	27.453353537693147	26.18514392344092	21.97305686635599
32	25.24821838977342	26.36277482632461	26.361469916110586	22.02753686779139
33	24.967010238651767	25.324555637293685	26.7610986691547	22.947335454899857
34	23.1040877943592	24.843696223426726	29.266363166299385	22.785852815914684
35	22.420314842211887	24.490065555426877	28.80948147761508	24.280138124746152
36	22.258016634342955	26.797473041370544	28.309374638091306	22.63513568619519
37	23.24355007348276	27.915944208563797	28.026372235425377	20.814133482528067
38	23.076032224757736	27.674862046523312	26.166222725337608	23.082883003381347
39	25.068467007791945	24.471959926207326	26.931715679638017	23.52785738636271
40	24.866205924618598	23.434066964729908	29.112546874821593	22.587180235829898
41	20.9943742058398	25.189986771472704	29.663871440245714	24.15176758244178
42	21.67618979266608	25.21869479618118	28.778326746255317	24.326788664897425
43	22.10534213930243	25.3469022247088	28.74162614648596	23.806129489502815
44	22.02460081980984	25.161441860540982	27.959332473180016	24.854624846469157
45	23.229359174905273	23.844787454593202	26.54089507053855	26.384958299962975
46	22.410528015606726	25.54981576298916	28.27104290055442	23.768613320849692
47	21.730180452771222	25.823520680380184	29.798114078513184	22.648184788335406
48	22.77052012089993	23.486915828397784	30.358573015435457	23.38399103526683
49	22.094902857590252	23.704509606585884	30.014402946487262	24.1861845893366
50	21.679941409531388	22.658787183824334	29.567144970631364	26.09412643601291
51	20.480728922845554	24.763933586594657	27.08651065377633	27.66882683678346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	583.5
2	1025.0
3	2076.0
4	3127.0
5	2252.0
6	1377.0
7	1337.0
8	1297.0
9	1424.0
10	1551.0
11	1536.0
12	1521.0
13	1429.0
14	1337.0
15	1342.5
16	1348.0
17	1353.5
18	1359.0
19	1444.0
20	1529.0
21	1800.5
22	2072.0
23	2292.0
24	2512.0
25	2964.0
26	4112.0
27	4808.0
28	6120.0
29	7432.0
30	8551.0
31	9670.0
32	10543.5
33	11417.0
34	14022.0
35	16627.0
36	17425.0
37	18223.0
38	20596.0
39	22969.0
40	25816.5
41	28664.0
42	32337.5
43	36011.0
44	38941.5
45	41872.0
46	45389.0
47	48906.0
48	53997.5
49	59089.0
50	56479.5
51	53870.0
52	47996.0
53	42122.0
54	38906.0
55	35690.0
56	34427.0
57	33164.0
58	32529.0
59	31894.0
60	29700.5
61	27507.0
62	24170.5
63	20834.0
64	17464.0
65	14094.0
66	11278.0
67	8462.0
68	6927.0
69	5392.0
70	4560.0
71	3728.0
72	3351.0
73	2974.0
74	2661.0
75	1591.5
76	835.0
77	702.0
78	569.0
79	339.5
80	110.0
81	87.5
82	65.0
83	76.5
84	88.0
85	48.0
86	8.0
87	8.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	613069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.80786599824408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27910684350466	18.653695038899347
2	11.47100941729462	5.920845478122709
3	4.370187647561992	3.383556515863842
4	2.1515651386375465	2.22109219137805
5	1.2974532886305128	1.6742250305978683
6	0.8031226005499708	1.2436128273092972
7	0.5644993584548987	1.0197966659369144
8	0.42254100647640586	0.8723905339125012
9	0.3275794844954939	0.7608714695670222
>10	4.6306030716317474	31.266024748154177
>50	1.5674971842118492	26.63559678739078
>100	0.1090932106228027	4.597601621342738
>500	0.002551887967784858	0.3999274580375845
>1k	0.003189859959731073	1.3507636334871733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1753	0.28593845064748014	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	1718	0.2802294684611357	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1689	0.27549916893530746	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1648	0.26881150408844684	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1396	0.22770683234676686	No Hit
GGGCTGGGCAGTTTCCTTTACCTGTAACTCCACTTTGTTTACAACTGAGGA	645	0.10520838600549041	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2999662354482122	0.0
2	0.0	0.0	0.0	1.1837166778943315	0.0
3	0.0	0.0	0.0	1.6931210026930084	0.0
4	0.0	0.0	0.0	2.2402046099215585	0.0
5	0.0	0.0	0.0	3.1394508611591845	0.0
6	0.0	0.0	0.0	3.7351423738600387	0.0
7	0.0	0.0	0.0	4.227419752099682	0.0
8	0.0	0.0	0.0	4.9669776158964165	0.0
9	0.0	0.0	0.0	5.357145769888871	0.0
10	0.0	0.0	0.0	6.128673933929133	0.0
11	0.0	0.0	0.0	7.467022472185024	0.0
12	0.0	0.0	0.0	8.644051485232493	0.0
13	0.0	0.0	0.0	9.053467064881767	0.0
14	0.0	0.0	0.0	9.194723595549604	0.0
15	0.0	0.0	0.0	9.447549949516286	0.0
16	0.0	0.0	0.0	10.033943976942236	0.0
17	0.0	0.0	0.0	10.807103278750027	0.0
18	0.0	0.0	0.0	11.726249410751482	0.0
19	0.0	0.0	0.0	12.274148586863795	0.0
20	0.0	0.0	0.0	12.885499022132908	0.0
21	0.0	0.0	0.0	13.655232934628891	0.0
22	0.0	0.0	0.0	14.46819199796434	0.0
23	0.0	0.0	0.0	15.199104831593182	0.0
24	0.0	0.0	0.0	15.816653590378897	0.0
25	0.004240958195570156	0.0	0.0	16.341390610192327	0.0
26	0.004240958195570156	0.0	0.0	16.78832235849472	0.0
27	0.004240958195570156	0.0	0.0	17.252217939579396	0.0
28	0.004240958195570156	0.0	0.0	17.73748142541867	0.0
29	0.004240958195570156	0.0	0.0	18.241829223138016	0.0
30	0.004240958195570156	0.0	0.0	18.80000456718575	0.0
31	0.004240958195570156	0.0	0.0	19.357038114796215	0.0
32	0.004240958195570156	0.0	0.0	19.885852979028463	0.0
33	0.004240958195570156	0.0	0.0	20.379109039928622	0.0
34	0.004240958195570156	0.0	0.0	20.88802402339704	0.0
35	0.004404071972322854	0.0	0.0	21.395470982874684	0.0
36	0.004404071972322854	0.0	0.0	21.93554069770287	0.0
37	0.004404071972322854	0.0	0.0	22.474957957424042	0.0
38	0.004404071972322854	0.0	0.0	23.016332582466248	0.0
39	0.004404071972322854	0.0	0.0	23.550530201331334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	85	0.0	45.000004	6
AAGTAGC	25	3.8886676E-5	45.000004	19
CCTGTCG	25	3.8886676E-5	45.000004	1
CCGTATG	25	3.8886676E-5	45.000004	35
TGCGCTC	25	3.8886676E-5	45.000004	13
CCCCGTA	25	3.8886676E-5	45.000004	22
CGTGTGA	25	3.8886676E-5	45.000004	12
GCTACGA	85	0.0	45.000004	10
GGCGAAG	25	3.8886676E-5	45.000004	42
TGTCGTG	25	3.8886676E-5	45.000004	1
CCCGCGA	25	3.8886676E-5	45.000004	28
CCCGACA	25	3.8886676E-5	45.000004	33
TTCTACG	20	7.0307084E-4	45.0	1
AACGTCC	20	7.0307084E-4	45.0	22
CGTATTA	20	7.0307084E-4	45.0	25
GTATTCG	20	7.0307084E-4	45.0	38
ACGCAGT	40	6.8066583E-9	45.0	19
GTTTACG	20	7.0307084E-4	45.0	1
TCGATAC	20	7.0307084E-4	45.0	25
CCTCGCG	20	7.0307084E-4	45.0	1
>>END_MODULE