##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1804234
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.223604587874966	33.0	31.0	34.0	30.0	34.0
2	32.4091514737002	34.0	31.0	34.0	30.0	34.0
3	32.47708667501001	34.0	31.0	34.0	30.0	34.0
4	35.96532766814061	37.0	35.0	37.0	35.0	37.0
5	35.925872697222204	37.0	35.0	37.0	35.0	37.0
6	35.55440868534791	37.0	35.0	37.0	33.0	37.0
7	35.90394150647865	37.0	35.0	37.0	35.0	37.0
8	35.997256453431206	37.0	35.0	37.0	35.0	37.0
9	37.78415770903331	39.0	38.0	39.0	35.0	39.0
10	37.25322879404778	39.0	37.0	39.0	34.0	39.0
11	37.1492411738167	39.0	37.0	39.0	34.0	39.0
12	37.033960672507	39.0	37.0	39.0	33.0	39.0
13	36.993069080839845	39.0	37.0	39.0	33.0	39.0
14	38.183636934011886	40.0	37.0	41.0	33.0	41.0
15	38.21775667679469	40.0	37.0	41.0	33.0	41.0
16	38.24152354960609	40.0	37.0	41.0	33.0	41.0
17	38.169969083832804	40.0	37.0	41.0	33.0	41.0
18	38.133300336874264	40.0	37.0	41.0	33.0	41.0
19	38.16943035105203	40.0	37.0	41.0	34.0	41.0
20	38.12626521836968	40.0	37.0	41.0	34.0	41.0
21	37.97402775914876	40.0	37.0	41.0	33.0	41.0
22	38.05456720137188	40.0	37.0	41.0	34.0	41.0
23	38.00065789692468	40.0	37.0	41.0	33.0	41.0
24	37.96032665385975	40.0	37.0	41.0	33.0	41.0
25	37.87639740743163	40.0	36.0	41.0	33.0	41.0
26	37.81985540678205	40.0	36.0	41.0	33.0	41.0
27	37.714682796133985	40.0	36.0	41.0	33.0	41.0
28	37.575035721530575	40.0	36.0	41.0	33.0	41.0
29	37.494775067978985	39.0	36.0	41.0	33.0	41.0
30	37.48913500133575	39.0	36.0	41.0	33.0	41.0
31	37.43573671707772	40.0	36.0	41.0	32.0	41.0
32	37.31652490752308	39.0	36.0	41.0	32.0	41.0
33	37.26219825144632	39.0	36.0	41.0	32.0	41.0
34	37.116673336163714	39.0	35.0	41.0	31.0	41.0
35	37.06434974620809	39.0	35.0	41.0	31.0	41.0
36	36.927866895314025	39.0	35.0	41.0	31.0	41.0
37	36.90561590126336	39.0	35.0	41.0	31.0	41.0
38	36.838006600030816	39.0	35.0	41.0	31.0	41.0
39	36.76187567688005	39.0	35.0	41.0	31.0	41.0
40	36.62445392338245	39.0	35.0	41.0	30.0	41.0
41	36.53909803273855	39.0	35.0	41.0	30.0	41.0
42	36.5080166984992	39.0	35.0	41.0	30.0	41.0
43	36.43795427865787	39.0	35.0	40.0	30.0	41.0
44	36.28446642730378	39.0	35.0	40.0	30.0	41.0
45	36.292540213741674	39.0	35.0	40.0	30.0	41.0
46	36.17451006909303	38.0	35.0	40.0	30.0	41.0
47	35.959306276236894	38.0	35.0	40.0	29.0	41.0
48	35.85956311653588	38.0	35.0	40.0	29.0	41.0
49	35.80451925858841	38.0	35.0	40.0	29.0	41.0
50	35.67571501257597	38.0	34.0	40.0	28.0	41.0
51	34.726730013956065	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	21.0
10	30.0
11	26.0
12	23.0
13	33.0
14	40.0
15	54.0
16	96.0
17	162.0
18	331.0
19	573.0
20	1031.0
21	1754.0
22	2737.0
23	4182.0
24	6427.0
25	9456.0
26	12852.0
27	16444.0
28	19624.0
29	23872.0
30	29774.0
31	38476.0
32	49443.0
33	67530.0
34	109856.0
35	134181.0
36	150354.0
37	226172.0
38	386329.0
39	512164.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.188381329694487	4.315571040120073	55.21329273253913	16.282754897646313
2	19.72355027119542	4.597685222648503	54.55223657241799	21.126527933738085
3	21.285210233262426	4.570249756960572	52.281466816388566	21.86307319338844
4	18.70095564100887	4.726659623973387	51.57773326519731	24.994651469820433
5	18.21299232804614	5.869305200988341	50.708389266580724	25.2093132043848
6	20.819694119498912	6.707888222924521	53.96223549716943	18.510182160407133
7	85.89224014179979	2.052893360839004	8.148665860415	3.9062006369462052
8	88.5221650850167	2.5641906759322795	5.647382767423738	3.266261471627294
9	83.30759757326378	5.098507178115478	7.36512004540431	4.2287752032164345
10	37.4629898339129	32.59848777930136	15.088730175797597	14.849792210988152
11	26.330730936231113	28.350757163427804	27.079691436920044	18.23882046342104
12	26.739768788305728	23.428502067913584	29.90698545754043	19.924743686240255
13	24.946764111528772	21.723069180605176	31.645895155506437	21.684271552359615
14	20.24903643318993	25.628161313887222	31.1174160336187	23.005386219304146
15	18.201075913656435	27.476923724971375	33.66808296484824	20.653917396523955
16	21.77927031637803	25.35103539784751	32.86048262032531	20.00921166544916
17	21.66127010132832	25.068034412387753	30.19414333174078	23.076552154543148
18	21.480362303337593	25.717285008485597	30.654504903465956	22.14784778471085
19	23.48686478583155	26.599764775522466	29.297585568169094	20.61578487047689
20	26.667383499036156	26.420076331562313	28.463325710523137	18.449214458878394
21	26.171605235241106	25.76234568243365	28.865435414696762	19.200613667628478
22	23.44779003166995	24.185998046816543	29.39873652752359	22.967475393989915
23	22.7727113001972	25.575008563190803	29.595107951629334	22.057172184982658
24	23.022956002381065	24.74629122386564	29.812651795720512	22.418100978032783
25	22.634425468093387	26.07150735436756	28.357574460962383	22.936492716576677
26	22.4524091664385	27.497431042758315	27.82721088284557	22.222948907957615
27	22.419098631330524	27.250345576017303	29.47538955590018	20.855166236751995
28	20.518347398397324	27.653674634221503	30.077362470721646	21.750615496659524
29	22.727927752165183	26.358388102651876	29.148935226805396	21.76474891837755
30	23.598213978896307	25.05190568407424	29.555478945635656	21.7944013913938
31	22.58786831419871	27.220083425985763	27.267582807994973	22.92446545182055
32	23.10880961117017	28.194125595682156	26.845298337133656	21.851766456014023
33	23.58651926523943	26.919124681166632	27.344734663020432	22.149621390573508
34	22.329697810816114	26.500886248679496	28.771600579525714	22.397815360978676
35	22.638526931650773	25.608152822749155	28.33041612119049	23.422904124409584
36	22.91936633496542	26.85183850875219	27.36158391871564	22.867211237566746
37	22.148291186176515	28.085104260312132	27.74723234347651	22.01937221003484
38	21.45586437235968	27.710208321093603	27.19514209354219	23.63878521300452
39	22.373317430000764	26.291323630970265	28.142857301214807	23.192501637814164
40	22.94264491191276	25.206486520041192	29.42417668661604	22.426691881430013
41	20.079269097024	26.069234921856033	29.659456589333754	24.19203939178621
42	22.208538360323548	26.54910615807041	27.719298051139706	23.523057430466338
43	22.538706176693267	25.652049567849843	27.759259608232632	24.04998464722425
44	22.163921087841157	25.73380171308156	28.096688123602593	24.00558907547469
45	23.107257706040347	24.862019006403827	27.38103815802163	24.649685129534195
46	22.48361354458457	25.98254993531881	27.779933201569197	23.75390331852742
47	21.1831724709766	26.249810168747512	29.16123961747756	23.40577774279833
48	21.111175157989486	24.73016249555213	29.671095877807424	24.48756646865096
49	21.50031536929245	24.781929616668346	30.134173283509792	23.58358173052941
50	21.147534078173898	24.759870393751587	28.756580354876366	25.336015173198156
51	19.8141150205572	25.019038550431926	28.203326176094674	26.963520252916197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	338.0
1	1336.5
2	2335.0
3	5383.0
4	8431.0
5	5977.5
6	3524.0
7	3409.0
8	3294.0
9	3403.5
10	3513.0
11	3491.5
12	3470.0
13	3427.0
14	3384.0
15	3177.0
16	2970.0
17	3282.5
18	3595.0
19	4630.5
20	5666.0
21	6388.0
22	7110.0
23	8410.5
24	9711.0
25	11595.5
26	17465.5
27	21451.0
28	25796.0
29	30141.0
30	34816.5
31	39492.0
32	44793.5
33	50095.0
34	55216.0
35	60337.0
36	63717.0
37	67097.0
38	74156.5
39	81216.0
40	87148.5
41	93081.0
42	96783.5
43	100486.0
44	104691.5
45	108897.0
46	115771.5
47	122646.0
48	134722.0
49	146798.0
50	153378.5
51	159959.0
52	152982.5
53	146006.0
54	129789.0
55	113572.0
56	105110.0
57	96648.0
58	89249.5
59	81851.0
60	76508.5
61	71166.0
62	62525.0
63	53884.0
64	43895.0
65	33906.0
66	27208.0
67	20510.0
68	16968.0
69	13426.0
70	10918.0
71	8410.0
72	6718.5
73	5027.0
74	4484.0
75	2877.5
76	1814.0
77	1348.5
78	883.0
79	635.0
80	387.0
81	271.0
82	155.0
83	126.0
84	97.0
85	59.5
86	22.0
87	15.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1804234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.551267678248124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91316204427633	16.93646128893985
2	11.407895952791296	5.373408224595824
3	4.449824090781881	3.143969948493636
4	2.1979239639531647	2.0705558244598863
5	1.2679054714845206	1.4930390574823669
6	0.8128141297206755	1.1485681885028354
7	0.5735324822776773	0.9455191908604171
8	0.4263258859977822	0.8032412047440051
9	0.32053712696362197	0.6794150110143178
>10	4.4880032092422235	27.672712632318287
>50	1.9701748988343384	31.520204823292353
>100	0.1642607110738184	6.100722523865023
>500	0.005491273433163796	0.8817814769727311
>1k	0.002148759169498877	1.230400604458443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4434	0.24575526234401965	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	3709	0.20557200451826094	No Hit
TCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	3518	0.19498579452554382	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	3449	0.19116145688419572	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	2221	0.12309933190484161	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7712591603971546E-4	0.0	0.0	0.23311832057260864	0.0
2	2.7712591603971546E-4	0.0	0.0	0.8319874251344338	0.0
3	2.7712591603971546E-4	0.0	0.0	1.155005392870326	0.0
4	2.7712591603971546E-4	0.0	0.0	1.5593875295554789	0.0
5	2.7712591603971546E-4	0.0	0.0	2.2368495439061675	0.0
6	2.7712591603971546E-4	0.0	0.0	2.6946061320205694	0.0
7	2.7712591603971546E-4	0.0	0.0	3.1044199366601006	0.0
8	2.7712591603971546E-4	0.0	0.0	3.7065037018479865	0.0
9	2.7712591603971546E-4	0.0	0.0	4.004192360857849	0.0
10	2.7712591603971546E-4	0.0	0.0	4.640750590001075	0.0
11	2.7712591603971546E-4	0.0	0.0	5.762002046297764	0.0
12	2.7712591603971546E-4	0.0	0.0	6.766638917124941	0.0
13	2.7712591603971546E-4	0.0	0.0	7.0787381237688685	0.0
14	2.7712591603971546E-4	0.0	0.0	7.19374537892535	0.0
15	2.7712591603971546E-4	0.0	0.0	7.3757616805802355	0.0
16	2.7712591603971546E-4	0.0	0.0	7.806470779289161	0.0
17	3.325510992476586E-4	0.0	0.0	8.441920504768229	0.0
18	3.325510992476586E-4	0.0	0.0	9.129747028378802	0.0
19	3.325510992476586E-4	0.0	0.0	9.625913268456308	0.0
20	3.325510992476586E-4	0.0	0.0	10.129949884549344	0.0
21	3.325510992476586E-4	0.0	0.0	10.84083328437442	0.0
22	3.325510992476586E-4	0.0	0.0	11.62393569791945	0.0
23	3.325510992476586E-4	0.0	0.0	12.354883014065804	0.0
24	3.325510992476586E-4	0.0	0.0	12.89361579484701	0.0
25	3.325510992476586E-4	0.0	0.0	13.373597881427797	0.0
26	3.8797628245560166E-4	0.0	0.0	13.817775299656253	0.0
27	3.8797628245560166E-4	0.0	0.0	14.252696712288982	0.0
28	3.8797628245560166E-4	0.0	0.0	14.722757691075548	0.0
29	3.8797628245560166E-4	0.0	0.0	15.227958236015949	0.0
30	3.8797628245560166E-4	0.0	0.0	15.783872823591619	0.0
31	3.8797628245560166E-4	0.0	0.0	16.324490060601896	0.0
32	3.8797628245560166E-4	0.0	0.0	16.83578737569517	0.0
33	3.8797628245560166E-4	0.0	0.0	17.343260353147098	0.0
34	4.4340146566354473E-4	0.0	0.0	17.82739932846848	0.0
35	4.4340146566354473E-4	0.0	0.0	18.352386663814116	0.0
36	4.4340146566354473E-4	0.0	0.0	18.87720772361013	0.0
37	4.4340146566354473E-4	0.0	0.0	19.415552528108883	0.0
38	4.4340146566354473E-4	0.0	0.0	19.941703792301887	0.0
39	4.4340146566354473E-4	0.0	0.0	20.496288175480565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCG	45	3.8562575E-10	45.000004	20
AACCGCG	20	7.034358E-4	45.0	32
GAACGTA	20	7.034358E-4	45.0	9
GTCCGAT	20	7.034358E-4	45.0	36
TATCGGA	50	1.0822987E-9	40.5	22
TTTGGGA	11935	0.0	39.627148	4
TAGCCGT	40	3.4596815E-7	39.375	44
ACGTAAG	40	3.4596815E-7	39.375	20
TTTGGGC	4380	0.0	39.34932	4
TTGGGAT	7130	0.0	39.28822	5
CGTTCAT	275	0.0	39.272728	17
CGTTTGG	1315	0.0	39.01141	2
CGTTTTT	2405	0.0	38.91892	1
TGGGATC	2315	0.0	38.682507	6
TGGGCGA	1955	0.0	38.670074	6
CGACGTG	35	6.2502695E-6	38.571426	21
CCAATCG	35	6.2502695E-6	38.571426	24
ACGATAG	35	6.2502695E-6	38.571426	26
GCGATCG	35	6.2502695E-6	38.571426	9
TTGGGAC	4165	0.0	38.463387	5
>>END_MODULE