##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1320757
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22423352668205	33.0	31.0	34.0	30.0	34.0
2	32.41257627254673	34.0	31.0	34.0	30.0	34.0
3	32.48257022298576	34.0	31.0	34.0	30.0	34.0
4	35.958345100574896	37.0	35.0	37.0	35.0	37.0
5	35.92057812300067	37.0	35.0	37.0	35.0	37.0
6	35.52356413783913	37.0	35.0	37.0	33.0	37.0
7	35.861154625718434	37.0	35.0	37.0	35.0	37.0
8	35.94103230192988	37.0	35.0	37.0	35.0	37.0
9	37.72353809216987	39.0	38.0	39.0	35.0	39.0
10	37.28867460100533	39.0	37.0	39.0	34.0	39.0
11	37.183024583628935	39.0	37.0	39.0	34.0	39.0
12	36.98083220456147	39.0	37.0	39.0	33.0	39.0
13	36.88749330876156	39.0	37.0	39.0	33.0	39.0
14	38.07846787864838	40.0	37.0	41.0	33.0	41.0
15	38.11927477953931	40.0	37.0	41.0	33.0	41.0
16	38.1629989468161	40.0	37.0	41.0	33.0	41.0
17	38.0806643462802	40.0	37.0	41.0	33.0	41.0
18	38.03293035736324	40.0	37.0	41.0	33.0	41.0
19	38.06829946765378	40.0	37.0	41.0	34.0	41.0
20	38.0101244967848	40.0	36.0	41.0	34.0	41.0
21	37.85943818582828	40.0	36.0	41.0	33.0	41.0
22	37.92402008847956	40.0	36.0	41.0	33.0	41.0
23	37.87195600704747	40.0	36.0	41.0	33.0	41.0
24	37.830785678213324	40.0	36.0	41.0	33.0	41.0
25	37.759901329313415	40.0	36.0	41.0	33.0	41.0
26	37.69163517588777	40.0	36.0	41.0	33.0	41.0
27	37.59752929569936	40.0	36.0	41.0	33.0	41.0
28	37.456027111724566	39.0	35.0	41.0	33.0	41.0
29	37.375514950895585	39.0	35.0	41.0	32.0	41.0
30	37.30144682178478	39.0	35.0	41.0	32.0	41.0
31	37.23111367193208	39.0	35.0	41.0	32.0	41.0
32	37.10939029662534	39.0	35.0	41.0	32.0	41.0
33	37.032757729090214	39.0	35.0	41.0	31.0	41.0
34	36.88510452717646	39.0	35.0	41.0	31.0	41.0
35	36.83540272737529	39.0	35.0	41.0	31.0	41.0
36	36.66893228655991	39.0	35.0	41.0	31.0	41.0
37	36.63197847900863	39.0	35.0	41.0	31.0	41.0
38	36.51554979454964	39.0	35.0	41.0	30.0	41.0
39	36.41500442549235	39.0	35.0	41.0	30.0	41.0
40	36.28175129868704	39.0	35.0	40.0	30.0	41.0
41	36.22945174623341	39.0	35.0	40.0	30.0	41.0
42	36.17600966718329	38.0	35.0	40.0	30.0	41.0
43	36.11490077281438	38.0	35.0	40.0	30.0	41.0
44	35.95523779166039	38.0	35.0	40.0	29.0	41.0
45	35.95442007878815	38.0	35.0	40.0	29.0	41.0
46	35.81458663478596	38.0	35.0	40.0	29.0	41.0
47	35.59588932710559	38.0	34.0	40.0	28.0	41.0
48	35.50552978329852	38.0	34.0	40.0	28.0	41.0
49	35.48110591123121	37.0	34.0	40.0	28.0	41.0
50	35.35905620791713	37.0	34.0	40.0	28.0	41.0
51	34.395052988551264	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	25.0
10	27.0
11	39.0
12	26.0
13	28.0
14	32.0
15	57.0
16	92.0
17	175.0
18	307.0
19	551.0
20	942.0
21	1617.0
22	2481.0
23	3665.0
24	5270.0
25	7682.0
26	10298.0
27	13074.0
28	15246.0
29	18042.0
30	22949.0
31	28374.0
32	37573.0
33	52164.0
34	89690.0
35	106778.0
36	112935.0
37	169047.0
38	276663.0
39	344785.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.65816421945899	4.555342125765754	52.621261897533	17.165231757242246
2	21.113346361215577	5.677501614604353	52.203925476071674	21.005226548108396
3	22.013057663143183	5.426887762094011	49.79848677690143	22.761567797861378
4	19.635784629572285	5.536900429072116	49.39038748233021	25.436927459025394
5	19.131982643287145	6.141023670516227	49.14499790650362	25.581995779693013
6	22.024339072213888	7.3863700892745605	51.824673274493335	18.76461756401821
7	83.15799196975674	2.7670495026715742	9.466616493420062	4.608342034151627
8	84.45823115077187	3.83893479269843	7.219041807084876	4.483792249444826
9	78.46765150591668	5.776990014060118	9.356376683977446	6.398981796045752
10	40.40599444106675	27.494383902564966	16.909469342202993	15.190152314165287
11	29.200375239351374	25.083190927627108	25.3564433124337	20.359990520587814
12	27.814806205834984	20.672841408374136	30.123785071742947	21.38856731404793
13	24.649878819495182	22.104141791412047	31.251623122194317	21.99435626689845
14	20.984329441373394	25.340164769143758	30.36440465581481	23.311101133668043
15	18.678757712433097	25.526345875887845	34.04744400370394	21.747452407975125
16	23.071465833609057	23.084261525776505	31.54274404754243	22.301528593072003
17	22.62020947078077	23.727377556961653	29.21627521186713	24.43613776039044
18	22.54994673509207	24.32657937834136	30.319051877067473	22.8044220094991
19	23.90432153681563	25.874025274899164	28.032105830217063	22.189547358068136
20	27.084846039051847	25.677244186477903	28.05535007575201	19.182559698718237
21	26.818786498954765	25.41868034770968	28.374409524234967	19.388123629100583
22	24.47755340308626	23.94990145802748	27.94624597863195	23.62629916025431
23	23.91954008193786	24.49845050982126	28.30081536573344	23.28119404250744
24	24.19483674892505	23.77083748183807	28.831571591140538	23.20275417809635
25	24.278273747555378	24.866724158948237	26.811366511780744	24.043635581715638
26	22.817217701666547	26.49185277836877	26.963779105467545	23.727150414497142
27	22.682900790985773	25.92376947462705	28.64311905975134	22.750210674635834
28	21.559529875669785	26.99065763043467	29.106565401508377	22.34324709238717
29	23.955882876259597	24.739448664667307	28.67226900936357	22.632399449709524
30	24.58961035224496	24.4561262972674	29.194772391893437	21.7594909585942
31	24.290085155709946	26.654486782958557	26.50820703581355	22.547221025517942
32	25.21697783922402	26.629955396791388	25.8398781910677	22.313188572916896
33	24.90253695418612	25.709498416438453	26.11615914206777	23.271805487307656
34	22.78466061508665	25.7306983797928	28.48411933459372	23.000521670526826
35	24.072406960553682	24.319007962857665	27.297375671679198	24.311209404909455
36	23.911060096596117	26.810836512696884	26.495941342729967	22.78216204797703
37	23.866085888622965	27.436992573198555	27.429951156798715	21.266970381379767
38	23.13923000218814	27.550336662989483	26.193236151691796	23.117197183130582
39	23.186324206496728	24.51215477184675	28.49456788796122	23.806953133695295
40	23.182689927064555	23.989045676078188	30.263931972346164	22.564332424511093
41	20.85447966582801	25.626137131962956	28.257885439940882	25.261497762268153
42	22.216728739654606	25.806942533713624	27.056831801762172	24.919496924869602
43	23.001278812075196	25.089550916633414	26.738832351446934	25.170337919844453
44	22.70500932419817	24.058626984373355	28.01764442664321	25.21871926478527
45	23.703300455723497	23.31791540760337	27.097868873683804	25.88091526298933
46	23.5189364886955	25.02587531241553	27.947154548489994	23.50803365039898
47	21.473821452394347	25.731758377960517	29.710915785416997	23.083504384228135
48	21.725419588917568	24.6947773133135	29.4444019603909	24.135401137378036
49	21.986633423105083	24.130706859778144	29.428577702029973	24.454082015086804
50	21.48737428611016	23.612443469919146	29.391856336934048	25.508325907036642
51	20.879162480304856	23.93945290465998	27.847287578260044	27.334097036775123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	361.0
1	1205.5
2	2050.0
3	4323.5
4	6597.0
5	4633.5
6	2670.0
7	2656.0
8	2642.0
9	2665.0
10	2688.0
11	2732.0
12	2776.0
13	2630.0
14	2484.0
15	2630.0
16	2776.0
17	2681.0
18	2586.0
19	2760.5
20	2935.0
21	3356.0
22	3777.0
23	4718.5
24	5660.0
25	6408.5
26	9971.5
27	12786.0
28	15681.5
29	18577.0
30	19853.5
31	21130.0
32	24333.0
33	27536.0
34	29850.5
35	32165.0
36	35762.0
37	39359.0
38	42886.0
39	46413.0
40	53503.5
41	60594.0
42	65329.5
43	70065.0
44	76331.5
45	82598.0
46	93857.0
47	105116.0
48	115436.5
49	125757.0
50	119942.0
51	114127.0
52	104582.5
53	95038.0
54	86161.5
55	77285.0
56	72963.0
57	68641.0
58	67351.5
59	66062.0
60	63058.0
61	60054.0
62	54685.5
63	49317.0
64	41654.0
65	33991.0
66	27750.5
67	21510.0
68	19092.5
69	16675.0
70	13732.5
71	10790.0
72	9423.0
73	8056.0
74	6704.5
75	3807.0
76	2261.0
77	1698.0
78	1135.0
79	881.5
80	628.0
81	481.5
82	335.0
83	232.0
84	129.0
85	86.5
86	44.0
87	37.0
88	30.0
89	30.5
90	31.0
91	16.0
92	1.0
93	3.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1320757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.71798328502251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70058283676397	17.97011791370526
2	11.096849395528073	5.485834757501501
3	4.36606901475628	3.237612627840013
4	2.1743470014552124	2.14981891351235
5	1.225113290049883	1.514116491285597
6	0.8124934703758993	1.2049920011964845
7	0.5595586874934174	0.9681813599115997
8	0.43040842838167703	0.851106267077688
9	0.3339385097075588	0.742885785105909
>10	4.360272197881958	27.8331328834166
>50	1.7948011443511662	29.808986010725715
>100	0.13689400484845243	5.4897796928144915
>500	0.005574868975630665	0.8867398993327616
>1k	0.0030971494309059247	1.8566953965740713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3367	0.25492955933604744	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3192	0.24167958223957928	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3175	0.24039244160735093	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	3118	0.23607673478164415	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3085	0.23357816767202444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	2107	0.15952972424147668	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	1944	0.14718831700305204	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	1766	0.133711197442073	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1457	0.11031552359745207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5142830967392184E-4	0.0	0.0	0.25977526524561295	0.0
2	1.5142830967392184E-4	0.0	0.0	1.0685538672140296	0.0
3	1.5142830967392184E-4	0.0	0.0	1.4932345616945433	0.0
4	1.5142830967392184E-4	0.0	0.0	2.009302241063269	0.0
5	1.5142830967392184E-4	0.0	0.0	2.9343020707064205	0.0
6	1.5142830967392184E-4	0.0	0.0	3.4807311261647675	0.0
7	1.5142830967392184E-4	0.0	0.0	3.983775970901536	0.0
8	1.5142830967392184E-4	0.0	0.0	4.719490413452285	0.0
9	1.5142830967392184E-4	0.0	0.0	5.066034100141056	0.0
10	1.5142830967392184E-4	0.0	0.0	5.8990412316573	0.0
11	1.5142830967392184E-4	0.0	0.0	7.167556181795743	0.0
12	1.5142830967392184E-4	0.0	0.0	8.384206935870868	0.0
13	1.5142830967392184E-4	0.0	0.0	8.754752009642955	0.0
14	1.5142830967392184E-4	0.0	0.0	8.899441759536387	0.0
15	1.5142830967392184E-4	0.0	0.0	9.140894199311456	0.0
16	1.5142830967392184E-4	0.0	0.0	9.6490118924223	0.0
17	1.5142830967392184E-4	0.0	0.0	10.338767842987014	0.0
18	1.5142830967392184E-4	0.0	0.0	11.138612174684669	0.0
19	1.5142830967392184E-4	0.0	0.0	11.687615511407474	0.0
20	1.5142830967392184E-4	0.0	0.0	12.229426003420766	0.0
21	1.5142830967392184E-4	0.0	0.0	12.951057613171841	0.0
22	1.5142830967392184E-4	0.0	0.0	13.705019167038296	0.0
23	1.5142830967392184E-4	0.0	0.0	14.412567943989696	0.0
24	2.2714246451088278E-4	0.0	0.0	15.009271198259786	0.0
25	3.028566193478437E-4	0.0	0.0	15.522310311435033	0.0
26	3.028566193478437E-4	0.0	0.0	15.977655238624516	0.0
27	3.028566193478437E-4	0.0	0.0	16.40786306640813	0.0
28	3.028566193478437E-4	0.0	0.0	16.86616084563625	0.0
29	3.028566193478437E-4	0.0	0.0	17.37268854149552	0.0
30	3.028566193478437E-4	0.0	0.0	17.917451885547454	0.0
31	3.028566193478437E-4	0.0	0.0	18.424055295561562	0.0
32	3.028566193478437E-4	0.0	0.0	18.9542815218848	0.0
33	3.028566193478437E-4	0.0	0.0	19.45702351000222	0.0
34	3.028566193478437E-4	0.0	0.0	19.963399777551814	0.0
35	3.028566193478437E-4	0.0	0.0	20.476363176572224	0.0
36	3.028566193478437E-4	0.0	0.0	21.008785113385734	0.0
37	3.028566193478437E-4	0.0	0.0	21.519477087761032	0.0
38	3.028566193478437E-4	0.0	0.0	22.039179046561934	0.0
39	3.028566193478437E-4	0.0	0.0	22.589242381452454	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	20	7.033668E-4	45.000004	43
ACTCGAT	20	7.033668E-4	45.000004	19
TCGTACT	20	7.033668E-4	45.000004	15
ACCGCGT	25	3.8911236E-5	45.0	23
ATTACGT	35	1.21212E-7	45.0	23
CTACGAT	25	3.8911236E-5	45.0	19
ACGGATC	25	3.8911236E-5	45.0	18
CGCGCAT	70	0.0	41.785713	36
GCCGATA	70	0.0	41.785713	9
CGTTCAT	260	0.0	41.53846	17
TCGTTCA	250	0.0	41.399998	16
CGTTTGG	860	0.0	40.552326	2
CGAACGA	95	0.0	40.263157	22
AAGTACG	45	1.9286745E-8	40.0	33
CGCGGAT	40	3.4587356E-7	39.375004	39
TTTGTCG	320	0.0	39.375004	1
CTTTGCG	570	0.0	39.07895	1
TTGGGAT	3325	0.0	39.04511	5
TGGGACG	300	0.0	39.0	6
CGGTAGT	255	0.0	38.82353	12
>>END_MODULE