##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934340.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988688
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16630726781351	33.0	31.0	34.0	30.0	34.0
2	32.3521545725244	34.0	31.0	34.0	30.0	34.0
3	32.43391949735407	34.0	31.0	34.0	30.0	34.0
4	35.93649766154743	37.0	35.0	37.0	35.0	37.0
5	35.89815695143463	37.0	35.0	37.0	35.0	37.0
6	35.525278955545126	37.0	35.0	37.0	33.0	37.0
7	35.90121959607075	37.0	35.0	37.0	35.0	37.0
8	35.98970757205509	37.0	35.0	37.0	35.0	37.0
9	37.77744040587122	39.0	38.0	39.0	35.0	39.0
10	37.24823503471267	39.0	37.0	39.0	34.0	39.0
11	37.142679996116065	39.0	37.0	39.0	34.0	39.0
12	36.958146553816775	39.0	37.0	39.0	33.0	39.0
13	36.88470781480103	39.0	37.0	39.0	33.0	39.0
14	38.04231264059036	40.0	37.0	41.0	33.0	41.0
15	38.09642677973233	40.0	37.0	41.0	33.0	41.0
16	38.11030274464745	40.0	37.0	41.0	33.0	41.0
17	38.05021098668134	40.0	37.0	41.0	33.0	41.0
18	38.04365077759617	40.0	37.0	41.0	33.0	41.0
19	38.08248507112456	40.0	37.0	41.0	34.0	41.0
20	38.04037370737786	40.0	37.0	41.0	34.0	41.0
21	37.87651008204813	40.0	36.0	41.0	33.0	41.0
22	37.95371037172495	40.0	36.0	41.0	33.0	41.0
23	37.90492046024631	40.0	36.0	41.0	33.0	41.0
24	37.863028579288915	40.0	36.0	41.0	33.0	41.0
25	37.789040627579176	40.0	36.0	41.0	33.0	41.0
26	37.69823948505494	40.0	36.0	41.0	33.0	41.0
27	37.62514059035813	39.0	36.0	41.0	33.0	41.0
28	37.469082258508244	39.0	36.0	41.0	33.0	41.0
29	37.388826404285275	39.0	36.0	41.0	32.0	41.0
30	37.36173292282297	39.0	36.0	41.0	32.0	41.0
31	37.294344626413995	39.0	35.0	41.0	32.0	41.0
32	37.16312021588206	39.0	35.0	41.0	32.0	41.0
33	37.140985831728514	39.0	35.0	41.0	32.0	41.0
34	37.01388911365365	39.0	35.0	41.0	31.0	41.0
35	36.94840030424158	39.0	35.0	41.0	31.0	41.0
36	36.78819607398896	39.0	35.0	41.0	31.0	41.0
37	36.762240464130244	39.0	35.0	41.0	31.0	41.0
38	36.68022672470992	39.0	35.0	41.0	30.0	41.0
39	36.65993720971631	39.0	35.0	41.0	30.0	41.0
40	36.59406910976971	39.0	35.0	41.0	30.0	41.0
41	36.56082100723383	39.0	35.0	41.0	30.0	41.0
42	36.49961868658262	39.0	35.0	40.0	30.0	41.0
43	36.40787488064991	39.0	35.0	40.0	30.0	41.0
44	36.23971161782079	39.0	35.0	40.0	30.0	41.0
45	36.2523849788811	38.0	35.0	40.0	30.0	41.0
46	36.14509329535708	38.0	35.0	40.0	30.0	41.0
47	35.95576562070137	38.0	35.0	40.0	29.0	41.0
48	35.872839561115335	38.0	35.0	40.0	29.0	41.0
49	35.896505267586946	38.0	35.0	40.0	29.0	41.0
50	35.81910875827359	38.0	35.0	40.0	29.0	41.0
51	34.9503240658327	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	17.0
11	13.0
12	18.0
13	8.0
14	18.0
15	34.0
16	46.0
17	70.0
18	161.0
19	302.0
20	585.0
21	951.0
22	1489.0
23	2278.0
24	3411.0
25	5080.0
26	6990.0
27	8916.0
28	10902.0
29	13061.0
30	16249.0
31	21238.0
32	27627.0
33	38641.0
34	65078.0
35	79997.0
36	81863.0
37	124865.0
38	212542.0
39	266134.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.99707895716343	5.037484019225478	52.3431052060913	16.622331817519783
2	19.40905523279336	4.772385221627045	53.292747560403285	22.525811985176315
3	21.39997653455893	5.1192084863981355	51.59817859628113	21.882636382761802
4	19.8455933519978	4.92157283187416	50.235564707976636	24.99726910815141
5	17.854874338517305	5.647383198744194	50.932953570792804	25.56478889194569
6	20.654341915750976	6.848166458984027	54.32876701244478	18.168724612820224
7	84.78690952049584	2.0923688767336106	8.715995339277912	4.404726263492629
8	85.81766947712524	2.6277248232000385	6.97985613257165	4.57474956710307
9	80.73274885504831	5.24391921415047	9.12532568413898	4.898006246662243
10	36.51910410564304	35.49067046429207	15.65893385982231	12.331291570242584
11	25.839799815513082	24.54363762885764	30.63180700079297	18.98475555483631
12	25.35855598530578	22.27244590811257	32.8596078843882	19.509390222193453
13	23.96974576408331	23.313320278996002	32.88701794701665	19.829916009904032
14	19.253192109138574	27.25541323450876	32.4807219264318	21.010672729920866
15	18.384262780573852	26.024489019791886	34.986568057870635	20.60468014176363
16	21.347988445293158	26.03976178531549	32.04114948295114	20.57110028644021
17	20.1126138883045	25.046728593853672	31.074514912692376	23.76614260514945
18	21.77087210525464	24.74066641852637	31.801943585843055	21.686517890375935
19	22.43478225688994	26.475996472092305	30.036169145372455	21.0530521256453
20	25.32790931011603	26.48327885035522	29.587594873205703	18.601216966323047
21	24.13946563526613	27.128578479763082	30.591855064489504	18.140100820481283
22	22.98015147346787	24.318086190992506	29.72100399721651	22.980758338323113
23	21.71554625928503	25.667854773194378	29.655159160422702	22.961439807097893
24	21.765916042270163	24.39182027090447	31.42862055572638	22.413643131098993
25	20.910438884663314	26.693658666839287	28.476324179114137	23.919578269383262
26	19.43100351172463	28.836093894130403	28.544191898758758	23.18871069538621
27	19.51343598789507	28.04767530302785	29.4076594436263	23.03122926545078
28	16.98321411810399	28.97385221627045	31.535529914391596	22.50740375123396
29	19.604061139611282	27.559452526985257	30.673377243377082	22.163109090026378
30	20.294976777304875	25.900789733464958	31.292480539866972	22.511752949363196
31	21.342830094023597	28.33795899211885	27.55702506756429	22.76218584629327
32	20.577472367420256	28.684681112747402	27.500687780169276	23.23715873966307
33	20.45215477481268	26.62558865890958	28.032503681646787	24.889752884630944
34	19.376992539608047	26.1584038645154	29.521952324696972	24.942651271179585
35	18.515649021733854	27.009430679850468	28.392273396663054	26.082646901752625
36	19.52092065444306	28.04413525803894	28.285970902853073	24.14897318466493
37	19.430396646869387	28.826687488874143	27.72482320003884	24.01809266421763
38	18.54811629148933	30.765519557231404	27.25966128849546	23.42670286278381
39	19.818689010082046	26.975142815529267	28.985989513375298	24.220178661013385
40	20.908517145955045	24.474859611930153	29.777745861181685	24.838877380933116
41	18.074357127830012	25.619609017202592	27.246209117537585	29.059824737429807
42	19.78035538005923	25.770617222015442	26.623565776058776	27.825461621866555
43	20.305192335701456	25.58471428802615	27.115328597090286	26.994764779182105
44	18.901109348955384	26.329640897836327	28.61256533911608	26.15668441409221
45	20.47875568430081	25.7419934296765	27.958466169307204	25.820784716715483
46	20.48371174728529	25.692938520544402	29.24947000469309	24.57387972747722
47	17.448982894502613	26.792476494101276	29.49120450536469	26.267336106031426
48	17.533640541808943	25.965926561260982	30.627862379233896	25.872570517696182
49	19.72705241694043	24.473443593934586	30.689863738611166	25.109640250513817
50	19.435656142281488	24.413363973265582	29.13790801547101	27.013071868981925
51	17.74189633129966	23.713446506885894	28.656664185263704	29.88799297655074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	183.0
1	641.5
2	1100.0
3	2556.0
4	4012.0
5	2841.0
6	1670.0
7	1692.5
8	1715.0
9	1825.0
10	1935.0
11	2000.0
12	2065.0
13	1992.5
14	1920.0
15	1818.5
16	1717.0
17	2319.0
18	2921.0
19	2944.0
20	2967.0
21	3266.5
22	3566.0
23	4252.0
24	4938.0
25	6556.5
26	9790.0
27	11405.0
28	14649.5
29	17894.0
30	19147.5
31	20401.0
32	23987.0
33	27573.0
34	30626.0
35	33679.0
36	36151.0
37	38623.0
38	41154.0
39	43685.0
40	47752.0
41	51819.0
42	55869.0
43	59919.0
44	66490.5
45	73062.0
46	81042.5
47	89023.0
48	96978.5
49	104934.0
50	98911.5
51	92889.0
52	81041.0
53	69193.0
54	59200.0
55	49207.0
56	44714.5
57	40222.0
58	37427.5
59	34633.0
60	32389.5
61	30146.0
62	26342.5
63	22539.0
64	18841.5
65	15144.0
66	12231.0
67	9318.0
68	7239.5
69	5161.0
70	4442.5
71	3724.0
72	3106.0
73	2488.0
74	1856.5
75	1095.5
76	966.0
77	749.5
78	533.0
79	402.5
80	272.0
81	163.5
82	55.0
83	57.0
84	59.0
85	34.5
86	10.0
87	6.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	988688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.437323450957745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.91247486803249	17.78386236475097
2	11.967697570165123	6.088523881110627
3	5.262786866513603	4.016136353308767
4	2.8092619621911195	2.8584042076291096
5	1.6415934670526717	2.0878871998198982
6	1.0679844575402835	1.6299999652228707
7	0.7304602727319558	1.30066679578903
8	0.5288417347186702	1.0761854608323524
9	0.4231576991406259	0.968759933742285
>10	3.8857650051493957	24.27003576554438
>50	1.563090557810616	27.49056918787161
>100	0.19885114909121054	7.510517108546326
>500	0.00562407290358979	0.9425699993092503
>1k	0.0024103169586813388	1.9758817765225138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	4882	0.49378570388231674	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3846	0.3890003722104446	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3659	0.3700864175553865	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3570	0.3610845888692894	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2023	0.20461460035926402	No Hit
TCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1356	0.13715145728480574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.011441425404172E-4	0.0	0.0	0.5384914148851812	0.0
2	1.011441425404172E-4	0.0	0.0	1.8094687100480638	0.0
3	1.011441425404172E-4	0.0	0.0	2.4870333209263187	0.0
4	1.011441425404172E-4	0.0	0.0	3.2314542100237893	0.0
5	1.011441425404172E-4	0.0	0.0	4.379743660285145	0.0
6	1.011441425404172E-4	0.0	0.0	5.038090884080721	0.0
7	1.011441425404172E-4	0.0	0.0	5.6341333160713996	0.0
8	1.011441425404172E-4	0.0	0.0	6.552724418623469	0.0
9	1.011441425404172E-4	0.0	0.0	6.991184276536178	0.0
10	1.011441425404172E-4	0.0	0.0	7.888029388442057	0.0
11	1.011441425404172E-4	0.0	0.0	9.645307720939265	0.0
12	1.011441425404172E-4	0.0	0.0	11.243385173077856	0.0
13	1.011441425404172E-4	0.0	0.0	11.737373165245254	0.0
14	1.011441425404172E-4	0.0	0.0	11.912757108410338	0.0
15	1.011441425404172E-4	0.0	0.0	12.190195491398702	0.0
16	1.011441425404172E-4	0.0	0.0	12.902755975595941	0.0
17	1.011441425404172E-4	0.0	0.0	13.93027931966404	0.0
18	1.011441425404172E-4	0.0	0.0	15.098494166005858	0.0
19	1.011441425404172E-4	0.0	0.0	15.850905442364022	0.0
20	1.011441425404172E-4	0.0	0.0	16.623039826517566	0.0
21	1.011441425404172E-4	0.0	0.0	17.658553557846357	0.0
22	1.011441425404172E-4	0.0	0.0	18.738368423607852	0.0
23	1.011441425404172E-4	0.0	0.0	19.707025876717427	0.0
24	1.011441425404172E-4	0.0	0.0	20.439107180424966	0.0
25	1.011441425404172E-4	0.0	0.0	21.058210476914862	0.0
26	1.011441425404172E-4	0.0	0.0	21.643025909083555	0.0
27	1.011441425404172E-4	0.0	0.0	22.219850953991553	0.0
28	1.011441425404172E-4	0.0	0.0	22.846236628744357	0.0
29	1.011441425404172E-4	0.0	0.0	23.478792096192127	0.0
30	1.011441425404172E-4	0.0	0.0	24.19782580551195	0.0
31	1.011441425404172E-4	0.0	0.0	24.867298884986972	0.0
32	1.011441425404172E-4	0.0	0.0	25.464453902545596	0.0
33	1.011441425404172E-4	0.0	0.0	26.06858786593951	0.0
34	2.022882850808344E-4	0.0	0.0	26.67980191931125	0.0
35	2.022882850808344E-4	0.0	0.0	27.341284611525577	0.0
36	2.022882850808344E-4	0.0	0.0	27.948756331623322	0.0
37	3.034324276212516E-4	0.0	0.0	28.589403330474326	0.0
38	3.034324276212516E-4	0.0	0.0	29.229241176185006	0.0
39	3.034324276212516E-4	0.0	0.0	29.871506481316654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	25	3.890408E-5	45.000004	10
TCTACGA	25	3.890408E-5	45.000004	31
GTTCGTA	25	3.890408E-5	45.000004	30
AACGCGC	25	3.890408E-5	45.000004	15
AGTTCGT	50	2.1827873E-11	45.000004	29
ACGTCGT	25	3.890408E-5	45.000004	10
GCCCGTT	25	3.890408E-5	45.000004	13
TCGAACC	25	3.890408E-5	45.000004	11
CGGACTA	25	3.890408E-5	45.000004	41
CGTTGGT	20	7.0328085E-4	45.0	13
ATTCGGA	20	7.0328085E-4	45.0	38
CGCGATT	20	7.0328085E-4	45.0	25
TTATCCG	20	7.0328085E-4	45.0	1
TCTAGCG	35	1.2117016E-7	45.0	1
ATCCGAT	35	1.2117016E-7	45.0	38
AACGCTC	20	7.0328085E-4	45.0	14
ACTCGAA	20	7.0328085E-4	45.0	45
TACTCGA	20	7.0328085E-4	45.0	44
CGTAATG	20	7.0328085E-4	45.0	21
CGAGAGT	20	7.0328085E-4	45.0	37
>>END_MODULE