SRR2934339.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1915330
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	5616	0.2932131799742081	TruSeq Adapter, Index 10 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5066	0.2644975017359933	RNA PCR Primer, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4971	0.25953752094939253	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	4576	0.23891444294194733	Illumina PCR Primer Index 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	4517	0.23583403382184792	Illumina PCR Primer Index 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2169	0.11324419290670536	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTT	20	7.034466E-4	45.0	45
GCTACGA	355	0.0	43.09859	10
CGATCAT	65	0.0	41.538464	10
TACGAAT	375	0.0	40.8	12
CGAATAT	375	0.0	40.2	14
TTTGGGC	4280	0.0	39.58528	4
TCTAGCG	40	3.459827E-7	39.375	1
CTACGAA	395	0.0	39.3038	11
CGTTTTT	2985	0.0	38.74372	1
CGAACGA	140	0.0	38.571426	22
ACGCATA	35	6.2504696E-6	38.571426	30
TGGGCGA	2170	0.0	38.36406	6
TTTGGGA	10815	0.0	38.34258	4
TTGGGAC	4350	0.0	38.22414	5
TTTCGCG	675	0.0	38.0	1
CGTTTGG	1300	0.0	37.903847	2
GGCCGAT	475	0.0	37.894737	8
GGTACGC	60	1.5643309E-10	37.499996	8
GGTACGA	30	1.14020615E-4	37.499996	8
ATGCGAC	30	1.14020615E-4	37.499996	16