##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934337.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2490101
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29497679009807	33.0	31.0	34.0	31.0	34.0
2	32.4925691769129	34.0	31.0	34.0	31.0	34.0
3	32.5489817481299	34.0	31.0	34.0	31.0	34.0
4	36.017959110895504	37.0	35.0	37.0	35.0	37.0
5	35.98133248410406	37.0	35.0	37.0	35.0	37.0
6	35.634231302264446	37.0	35.0	37.0	35.0	37.0
7	35.995421470856	37.0	35.0	37.0	35.0	37.0
8	36.086267183539945	37.0	35.0	37.0	35.0	37.0
9	37.8744223627877	39.0	38.0	39.0	35.0	39.0
10	37.35696865307873	39.0	37.0	39.0	35.0	39.0
11	37.22042198288342	39.0	37.0	39.0	34.0	39.0
12	37.04662903231636	39.0	37.0	39.0	33.0	39.0
13	37.02451587305093	39.0	37.0	39.0	33.0	39.0
14	38.210909918914936	40.0	37.0	41.0	33.0	41.0
15	38.23367405579131	40.0	37.0	41.0	33.0	41.0
16	38.24887143131945	40.0	37.0	41.0	34.0	41.0
17	38.15375922502742	40.0	37.0	41.0	33.0	41.0
18	38.11753057406105	40.0	37.0	41.0	33.0	41.0
19	38.163101014778114	40.0	37.0	41.0	34.0	41.0
20	38.12174044345993	40.0	37.0	41.0	34.0	41.0
21	37.98321313071237	40.0	36.0	41.0	33.0	41.0
22	38.0470896562027	40.0	37.0	41.0	34.0	41.0
23	38.000006827032315	40.0	36.0	41.0	34.0	41.0
24	37.96133369690627	40.0	36.0	41.0	34.0	41.0
25	37.903208343757946	40.0	36.0	41.0	33.0	41.0
26	37.85462075634683	40.0	36.0	41.0	33.0	41.0
27	37.78364532201706	40.0	36.0	41.0	33.0	41.0
28	37.7013325965493	40.0	36.0	41.0	33.0	41.0
29	37.62600673627295	40.0	36.0	41.0	33.0	41.0
30	37.576233654779465	40.0	36.0	41.0	33.0	41.0
31	37.51346993555683	40.0	36.0	41.0	33.0	41.0
32	37.388207145011386	39.0	35.0	41.0	33.0	41.0
33	37.327083519905415	40.0	35.0	41.0	32.0	41.0
34	37.13908391667647	39.0	35.0	41.0	31.0	41.0
35	37.083682147832555	39.0	35.0	41.0	31.0	41.0
36	36.976710181635205	39.0	35.0	41.0	31.0	41.0
37	36.98718766829137	39.0	35.0	41.0	31.0	41.0
38	36.9381559221895	39.0	35.0	41.0	31.0	41.0
39	36.88739533055085	39.0	35.0	41.0	31.0	41.0
40	36.77861018488808	39.0	35.0	41.0	31.0	41.0
41	36.66089809208542	39.0	35.0	41.0	31.0	41.0
42	36.594445365870705	39.0	35.0	41.0	31.0	41.0
43	36.55960581518581	39.0	35.0	41.0	30.0	41.0
44	36.44644253385706	39.0	35.0	41.0	30.0	41.0
45	36.46240453700472	39.0	35.0	40.0	30.0	41.0
46	36.35041751318521	39.0	35.0	40.0	30.0	41.0
47	36.17000756194226	38.0	35.0	40.0	30.0	41.0
48	36.09771772309637	38.0	35.0	40.0	30.0	41.0
49	36.098748604976265	38.0	35.0	40.0	30.0	41.0
50	36.01704147743405	38.0	35.0	40.0	30.0	41.0
51	35.10623304034656	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	28.0
10	49.0
11	39.0
12	39.0
13	38.0
14	36.0
15	71.0
16	130.0
17	225.0
18	421.0
19	717.0
20	1305.0
21	2222.0
22	3511.0
23	5302.0
24	8037.0
25	12114.0
26	17082.0
27	21536.0
28	25678.0
29	30225.0
30	38014.0
31	48842.0
32	65003.0
33	91040.0
34	161589.0
35	190603.0
36	193922.0
37	295351.0
38	529039.0
39	747635.0
40	246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.011315605270628	4.276533361498188	55.76500712220107	16.947143911030118
2	20.75546333261181	4.1011991079879895	54.06383114580493	21.079506413595272
3	21.015051196718527	4.38929987177227	52.49935645180658	22.09629247970263
4	18.926862805966504	4.797797358420401	51.63654004395806	24.638799791655035
5	18.16384154698946	5.644750955884922	51.5219663780706	24.669441119055012
6	21.54607383395292	6.261513087220156	53.72067237433341	18.471740704493513
7	85.69327107615314	1.8007703301994578	8.489334368364979	4.016624225282428
8	87.97952372213015	2.250551282859611	6.14047382013822	3.6294511748720235
9	82.52139170258556	5.201756876528302	8.07802575076272	4.19882567012342
10	38.0549624292348	35.46611161555294	14.266489592189233	12.212436363023025
11	26.60141094678489	25.075328269817167	30.016734260979778	18.306526522418167
12	26.206808478852867	21.89951331291381	32.27318891884305	19.62048928939027
13	24.975573279959328	21.64554771071535	32.75320960876687	20.62566940055845
14	19.55266071536857	24.57526823209179	32.92488939203671	22.947181660502928
15	18.55294223005412	26.318410377731666	34.72409352070458	20.404553871509627
16	23.514949795209112	25.23885577332004	32.021030472257955	19.2251639592129
17	21.647555661396865	24.812808797715434	31.59060616416764	21.949029376720063
18	21.921118862246953	25.272790139837703	31.728833489083375	21.07725750883197
19	23.264839458319162	26.949710072001093	29.739355953834806	20.04609451584494
20	25.54221696228386	27.68538304269586	28.960672679541915	17.811727315478368
21	25.78919489611064	26.648477310759684	29.47792077510109	18.084407018028585
22	22.225765139646946	24.87722385557855	30.9272997360348	21.969711268739704
23	22.521696911089148	25.33021752932913	30.77300880566692	21.3750767539148
24	23.852486304772377	24.148056645091906	30.492538254472407	21.506918795663307
25	22.826825096652705	25.86019603220914	27.96581343487674	23.34716543626142
26	20.514388773788696	26.656991021649322	29.594823663779103	23.233796540782883
27	19.354757096198107	26.99424641811718	31.241423540651564	22.409572945033155
28	18.544026929028178	27.301864462525817	30.99528894611102	23.15881966233498
29	20.004288982655723	26.51470763635692	30.545548152464498	22.93545522852286
30	22.250342455988733	25.348931629680884	31.095726639200578	21.304999275129806
31	22.398127626148497	26.944971308392713	27.958263540314228	22.698637525144562
32	22.39150138889949	27.159059010056218	28.40864687817884	22.040792722865458
33	23.27664620832649	27.10926183315456	27.52197601623388	22.092115942285073
34	20.620569205827394	25.840799228625666	30.727789756319122	22.81084180922782
35	19.232272104625476	26.56458513128584	31.262346386752988	22.940796377335698
36	19.0507533630162	30.236926132715098	28.933766140409567	21.778554363859136
37	20.51599513433391	29.43888621385237	27.878788852339724	22.166329799473996
38	20.580771623319695	28.471897324646672	27.309012766952023	23.63831828508161
39	21.20950114071678	27.078459869700065	27.6888768768817	24.023162112701453
40	22.99404722940957	25.725060951342936	29.258491924624746	22.022399894622747
41	19.12219624826463	26.93163851586743	30.457961343736663	23.48820389213128
42	19.809437448521162	28.146207724104364	28.865295022169786	23.179059805204687
43	19.981639298968197	26.46097487611948	28.93774188275897	24.61964394215335
44	20.1532789232244	25.41575622836182	29.154520238335714	25.276444610078062
45	22.23050390325533	24.43977172010292	28.033642008898436	25.296082367743317
46	20.685064581717768	26.834855293018233	28.605305567926763	23.874774557337233
47	18.29676788210599	27.03918435436956	30.76525811603626	23.898789647488194
48	18.653701195252722	25.577556894278587	31.804452911749365	23.96428899871933
49	21.25279255741032	24.879874350478154	30.07580817003005	23.791524922081475
50	20.081153334744254	24.33913323194521	29.207369500273284	26.372343933037257
51	18.191189835271743	24.386119277892746	29.66751147845007	27.755179408385445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	414.0
1	1725.5
2	3037.0
3	8877.0
4	14717.0
5	10008.5
6	5300.0
7	5231.5
8	5163.0
9	5190.5
10	5218.0
11	5139.0
12	5060.0
13	5021.5
14	4983.0
15	5126.5
16	5270.0
17	5464.5
18	5659.0
19	6479.5
20	7300.0
21	7992.5
22	8685.0
23	12341.5
24	15998.0
25	18576.5
26	26177.0
27	31199.0
28	37747.0
29	44295.0
30	49319.0
31	54343.0
32	62147.0
33	69951.0
34	78597.5
35	87244.0
36	92389.0
37	97534.0
38	107320.5
39	117107.0
40	128097.0
41	139087.0
42	149651.5
43	160216.0
44	177879.0
45	195542.0
46	213340.5
47	231139.0
48	230170.5
49	229202.0
50	204165.0
51	179128.0
52	164887.5
53	150647.0
54	136089.0
55	121531.0
56	114718.5
57	107906.0
58	104740.5
59	101575.0
60	95116.0
61	88657.0
62	77545.0
63	66433.0
64	54687.0
65	42941.0
66	34106.5
67	25272.0
68	19736.5
69	14201.0
70	12366.5
71	10532.0
72	8951.5
73	7371.0
74	5988.0
75	3412.0
76	2219.0
77	1685.0
78	1151.0
79	868.0
80	585.0
81	460.0
82	335.0
83	233.0
84	131.0
85	78.5
86	26.0
87	22.5
88	19.0
89	13.5
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2490101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.23578524749215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73536602835352	16.900913552184356
2	11.970400836819719	5.802241280151261
3	5.083112161537887	3.6957964420784353
4	2.5446250172507097	2.4668394181393682
5	1.572495419388436	1.905533064348162
6	0.9961785923189718	1.448590225895498
7	0.6755217150209873	1.146025945068637
8	0.5039925232105635	0.9771723647098336
9	0.38502790434093465	0.8398308243508958
>10	4.753666277089554	29.554062546907794
>50	1.6030235598551057	26.121257396137025
>100	0.16820532176904057	6.6078093730218015
>500	0.0058692501313214246	0.8970838388808461
>1k	0.0020123143307387743	0.8561576654674898
>5k	5.030785826846936E-4	0.7806860626585499
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7542	0.3028792808002567	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	6559	0.26340297040160215	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	5108	0.20513224162393412	TruSeq Adapter, Index 15 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	4170	0.1674630868386463	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	3742	0.1502750290048476	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2047704089111246E-4	0.0	0.0	0.22717954010700772	0.0
2	1.6063605452148327E-4	0.0	0.0	0.8858676816723499	0.0
3	1.6063605452148327E-4	0.0	0.0	1.2417970194783263	0.0
4	1.6063605452148327E-4	0.0	0.0	1.720773575047759	0.0
5	1.6063605452148327E-4	0.0	0.0	2.496926831481936	0.0
6	1.6063605452148327E-4	0.0	0.0	3.136017374395657	0.0
7	1.6063605452148327E-4	0.0	0.0	3.6640280856077725	0.0
8	1.6063605452148327E-4	0.0	0.0	4.504234968782391	0.0
9	1.6063605452148327E-4	0.0	0.0	4.949959861065876	0.0
10	2.007950681518541E-4	0.0	0.0	5.733422057980781	0.0
11	2.007950681518541E-4	0.0	0.0	7.257898374403287	0.0
12	2.007950681518541E-4	0.0	0.0	8.621738636304311	0.0
13	2.007950681518541E-4	0.0	0.0	9.044572890818484	0.0
14	2.007950681518541E-4	0.0	0.0	9.19669523445033	0.0
15	2.007950681518541E-4	0.0	0.0	9.41564217676311	0.0
16	2.007950681518541E-4	0.0	0.0	9.904457690671984	0.0
17	2.007950681518541E-4	0.0	0.0	10.608645994680536	0.0
18	2.007950681518541E-4	0.0	0.0	11.393433439045243	0.0
19	2.007950681518541E-4	0.0	0.0	12.006741895208267	0.0
20	2.007950681518541E-4	0.0	0.0	12.68824035651566	0.0
21	2.007950681518541E-4	0.0	0.0	13.567521959952629	0.0
22	2.007950681518541E-4	0.0	0.0	14.547923959710872	0.0
23	2.007950681518541E-4	0.0	0.0	15.429936376074705	0.0
24	2.007950681518541E-4	0.0	0.0	16.08561259161777	0.0
25	2.007950681518541E-4	0.0	0.0	16.67562881987518	0.0
26	2.007950681518541E-4	0.0	0.0	17.19018626152112	0.0
27	2.007950681518541E-4	0.0	0.0	17.723939711682377	0.0
28	2.409540817822249E-4	0.0	0.0	18.286969082780175	0.0
29	2.409540817822249E-4	0.0	0.0	18.901602786393003	0.0
30	2.409540817822249E-4	0.0	0.0	19.580852342937092	0.0
31	2.409540817822249E-4	0.0	0.0	20.173599384121367	0.0
32	2.409540817822249E-4	0.0	0.0	20.779036673612836	0.0
33	2.409540817822249E-4	0.0	0.0	21.38029742568675	0.0
34	2.8111309541259573E-4	0.0	0.0	21.97501225853891	0.0
35	3.2127210904296655E-4	0.0	0.0	22.616512342270454	0.0
36	3.2127210904296655E-4	0.0	0.0	23.19452102545238	0.0
37	3.2127210904296655E-4	0.0	0.0	23.820800843018013	0.0
38	3.2127210904296655E-4	0.0	0.0	24.45704009596398	0.0
39	3.2127210904296655E-4	0.0	0.0	25.11143122307087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACT	30	2.1665273E-6	45.000004	36
ACTACGT	20	7.034873E-4	45.000004	14
TACGGGT	195	0.0	42.692307	4
TATAGCG	60	3.6379788E-12	41.250004	34
CGTTTTT	4500	0.0	40.65	1
TCTAGCG	100	0.0	40.500004	1
TCTTGCG	765	0.0	40.294117	1
CGTACCG	40	3.460391E-7	39.375004	26
CGTTCAT	380	0.0	39.078945	17
TTTGGGA	17245	0.0	38.724266	4
TCGTTCA	390	0.0	38.653847	16
TCGTCTA	35	6.2512263E-6	38.571426	12
TTGGGAC	6720	0.0	38.4375	5
AACGACG	130	0.0	38.076927	11
TATTGCG	195	0.0	38.076923	1
TGGGATC	4530	0.0	37.847683	6
TTGGGAT	12090	0.0	37.797768	5
ACACGAC	485	0.0	37.577316	26
CACTACG	30	1.1403044E-4	37.500004	34
TGGGCGA	2910	0.0	37.500004	6
>>END_MODULE