SRR2934335.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319251
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1776	0.55630209459015	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1739	0.544712467619522	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1717	0.5378213380694188	TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1639	0.5133891514826892	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.2643687881948686	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	600	0.18793989682099665	No Hit
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	566	0.1772899693344735	No Hit
GCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	444	0.1390755236475375	TruSeq Adapter, Index 15 (95% over 21bp)
TTGTCTGGGCAGACTATAGGCAATAATCACACTATAAATAATCCACCTATA	405	0.12685943035417274	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	375	0.11746243551312291	No Hit
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	356	0.11151100544712468	No Hit
GGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	321	0.1005478447992332	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCATA	20	7.025624E-4	45.000004	32
AGGTAGC	20	7.025624E-4	45.000004	35
AGGTACA	20	7.025624E-4	45.000004	12
GGTACTC	20	7.025624E-4	45.000004	12
TCGTTCA	20	7.025624E-4	45.000004	16
CCCCTGA	20	7.025624E-4	45.000004	17
GCGACTC	40	6.7884685E-9	45.000004	13
ACGCCCC	20	7.025624E-4	45.000004	22
CTCGTGG	40	6.7884685E-9	45.000004	2
TCCGCAA	20	7.025624E-4	45.000004	19
CCTAGAT	20	7.025624E-4	45.000004	35
CTATACT	20	7.025624E-4	45.000004	17
GACCGTT	20	7.025624E-4	45.000004	12
GACCGTC	20	7.025624E-4	45.000004	31
GGTCCGC	20	7.025624E-4	45.000004	17
GGATCGT	20	7.025624E-4	45.000004	8
TTCGTGT	20	7.025624E-4	45.000004	23
TGTATCG	55	1.8189894E-12	45.000004	14
TGTATCC	20	7.025624E-4	45.000004	21
CCTAAGT	20	7.025624E-4	45.000004	43