##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1207070
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11431897073078	33.0	31.0	34.0	30.0	34.0
2	32.30085164903444	34.0	31.0	34.0	30.0	34.0
3	32.368628165723614	34.0	31.0	34.0	30.0	34.0
4	35.89236746833241	37.0	35.0	37.0	35.0	37.0
5	35.866358206234935	37.0	35.0	37.0	35.0	37.0
6	35.4992278823929	37.0	35.0	37.0	33.0	37.0
7	35.85518652605069	37.0	35.0	37.0	35.0	37.0
8	35.96209996106274	37.0	35.0	37.0	35.0	37.0
9	37.77387723992809	39.0	38.0	39.0	35.0	39.0
10	37.093959753783956	39.0	37.0	39.0	33.0	39.0
11	37.024578524857716	39.0	37.0	39.0	33.0	39.0
12	36.83987672628762	39.0	37.0	39.0	33.0	39.0
13	36.83444290720505	39.0	37.0	39.0	33.0	39.0
14	38.030669306668216	40.0	37.0	41.0	33.0	41.0
15	38.059493649912596	40.0	37.0	41.0	33.0	41.0
16	38.07746278177736	40.0	37.0	41.0	33.0	41.0
17	38.03319691484338	40.0	37.0	41.0	33.0	41.0
18	38.011752425294304	40.0	37.0	41.0	33.0	41.0
19	38.0470287555817	40.0	37.0	41.0	34.0	41.0
20	38.00373217791843	40.0	37.0	41.0	33.0	41.0
21	37.83118791785066	40.0	36.0	41.0	33.0	41.0
22	37.89769773086896	40.0	37.0	41.0	33.0	41.0
23	37.85343766310156	40.0	36.0	41.0	33.0	41.0
24	37.82212464894331	40.0	36.0	41.0	33.0	41.0
25	37.75190916848236	40.0	36.0	41.0	33.0	41.0
26	37.688969156718336	39.0	36.0	41.0	33.0	41.0
27	37.612544425758244	39.0	36.0	41.0	33.0	41.0
28	37.50248535710439	39.0	36.0	41.0	33.0	41.0
29	37.4214022384783	39.0	36.0	41.0	32.0	41.0
30	37.40915936938206	39.0	36.0	41.0	32.0	41.0
31	37.35910096349011	39.0	36.0	41.0	32.0	41.0
32	37.2199698443338	39.0	36.0	41.0	32.0	41.0
33	37.194322615921195	39.0	36.0	41.0	32.0	41.0
34	37.06959745499432	39.0	35.0	41.0	31.0	41.0
35	37.01005989710622	39.0	35.0	41.0	31.0	41.0
36	36.85545494461796	39.0	35.0	41.0	31.0	41.0
37	36.80635091585409	39.0	35.0	41.0	31.0	41.0
38	36.72656183982702	39.0	35.0	41.0	30.0	41.0
39	36.649943251012786	39.0	35.0	41.0	30.0	41.0
40	36.57740313320686	39.0	35.0	40.0	30.0	41.0
41	36.54817284830209	39.0	35.0	40.0	30.0	41.0
42	36.47966563662422	39.0	35.0	40.0	30.0	41.0
43	36.38449799928753	39.0	35.0	40.0	30.0	41.0
44	36.21980829612201	39.0	35.0	40.0	30.0	41.0
45	36.27012517915283	39.0	35.0	40.0	30.0	41.0
46	36.17944029758009	38.0	35.0	40.0	30.0	41.0
47	35.991783409412875	38.0	35.0	40.0	29.0	41.0
48	35.880918256604836	38.0	35.0	40.0	28.0	41.0
49	35.866437737662274	38.0	35.0	40.0	28.0	41.0
50	35.757859941842646	38.0	34.0	40.0	28.0	41.0
51	34.7590413149196	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	19.0
11	15.0
12	15.0
13	16.0
14	18.0
15	33.0
16	46.0
17	90.0
18	171.0
19	354.0
20	672.0
21	1206.0
22	1804.0
23	2747.0
24	4250.0
25	6544.0
26	9055.0
27	11096.0
28	13052.0
29	16032.0
30	20339.0
31	26070.0
32	34307.0
33	47308.0
34	74282.0
35	93416.0
36	107019.0
37	159841.0
38	266718.0
39	310402.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.540416048779274	4.5752110482407815	56.91782580960507	13.966547093374867
2	21.08684666175118	4.23521419635978	53.762333584630554	20.915605557258484
3	21.311274408277896	4.664186832578061	52.76421417150621	21.260324587637832
4	18.468688642746486	4.836836306096581	51.98944551683001	24.705029534326925
5	17.130158151557076	5.932878789134019	53.43202962545669	23.50493343385222
6	20.214900544293204	6.866958834201828	56.70483070575857	16.213309915746393
7	85.17815868176659	2.160023859428202	8.646640211421044	4.015177247384162
8	87.8304489383383	2.1064229912101204	6.38024306792481	3.68288500252678
9	83.32051993670623	4.248138053302625	8.190742873238504	4.240599136752633
10	38.29661908588566	22.96213144225273	21.834524923989495	16.90672454787212
11	28.732716412469866	27.84991756898937	22.75543257640402	20.661933442136746
12	22.411541998392803	22.5726759839943	33.52465060021374	21.491131417399156
13	24.04756973497809	23.76490178697176	32.43904661701475	19.7484818610354
14	22.879120514965994	25.93072481297688	31.235388171357087	19.954766500700043
15	20.66151921595268	27.076722973812622	32.46456294995319	19.797194860281508
16	21.85299941179882	25.64084932936781	30.755217178788307	21.750934080045067
17	21.43471381112943	25.6717506026991	29.292004606195164	23.601530979976307
18	21.80064122213293	25.643666067419453	31.31342838443504	21.242264326012574
19	23.140911463295417	28.170777171166545	28.555758986637063	20.132552378900975
20	24.01567431880504	27.218885400183918	29.06583710969538	19.699603171315665
21	23.621165301101012	27.460462110730944	29.450239008508206	19.46813357965984
22	22.990298822769184	25.23590181182533	28.849196815429096	22.924602549976388
23	21.054702709867694	26.860082679546338	28.807608506548917	23.277606104037048
24	21.255105337718607	26.548501743892235	29.775655098710104	22.420737819679058
25	20.78976364253937	27.195937269586683	28.46346939282726	23.550829695046684
26	20.709403762830654	28.910088064486732	28.312608216590583	22.067899956092024
27	21.430405858815146	27.02908696264508	29.333012998417658	22.207494180122115
28	19.25323303536663	26.71692611033329	30.884124367269507	23.14571648703058
29	20.32525039972827	27.4184595756667	29.660831600487132	22.595458424117908
30	21.42220418037065	27.782730081933938	28.630651080716113	22.164414656979297
31	21.43554226349756	28.725508876867124	26.824624918190327	23.014323941444985
32	19.776897777262295	29.70068015939424	26.71468928893933	23.807732774404137
33	21.75847299659506	28.181132825768184	26.62438798081304	23.436006196823715
34	20.646938454273574	27.93624230574863	26.903907809820478	24.51291143015732
35	20.99455706794138	27.40553571872385	26.868947119885345	24.73096009344943
36	23.418028780435268	27.857207949828926	26.042565882674573	22.68219738706123
37	21.64472648645066	27.921661544069526	26.78949853778157	23.644113431698244
38	19.996603345290662	29.48230011515488	26.31926897363036	24.2018275659241
39	21.307463527384492	28.689388353616607	27.726312475664212	22.276835643334685
40	22.220086656117708	27.292783351421214	27.425335730322185	23.0617942621389
41	21.767006055986812	26.271135891041947	26.54087998210543	25.420978070865814
42	22.71094468423538	25.91622689653458	26.51022724448458	24.862601174745457
43	22.44476293835486	25.84025781437696	27.660781893345042	24.054197353923136
44	20.91601978344255	27.002245105917634	26.831749608556255	25.249985502083554
45	21.729311473236844	27.31192060112504	25.953838634047738	25.00492929159038
46	22.277664095702818	27.540242073781968	26.95005260672538	23.23204122378984
47	20.819753618265718	28.029608887637004	27.040271069614853	24.110366424482425
48	20.685130108444415	26.86911281035897	27.450023610892494	24.995733470304124
49	22.02722294481679	25.88226034944121	28.42163254823664	23.668884157505367
50	22.046691575467868	25.210551169360517	26.82313370392769	25.91962355124392
51	20.65762548982246	25.39612449982188	25.90438002767031	28.04186998268535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	444.0
2	774.0
3	2494.5
4	4215.0
5	3038.5
6	1862.0
7	1930.5
8	1999.0
9	2159.0
10	2319.0
11	2350.0
12	2381.0
13	2320.0
14	2259.0
15	2218.5
16	2178.0
17	2237.5
18	2297.0
19	2443.5
20	2590.0
21	3204.0
22	3818.0
23	4686.5
24	5555.0
25	5787.0
26	8096.0
27	10173.0
28	11891.5
29	13610.0
30	16990.0
31	20370.0
32	23234.0
33	26098.0
34	30679.5
35	35261.0
36	40431.5
37	45602.0
38	49940.0
39	54278.0
40	58914.0
41	63550.0
42	69283.5
43	75017.0
44	81179.5
45	87342.0
46	93267.5
47	99193.0
48	110602.5
49	122012.0
50	131016.0
51	140020.0
52	128913.0
53	117806.0
54	99970.0
55	82134.0
56	69373.0
57	56612.0
58	47959.5
59	39307.0
60	32724.5
61	26142.0
62	22997.0
63	19852.0
64	16477.0
65	13102.0
66	10699.0
67	8296.0
68	6655.0
69	5014.0
70	4172.0
71	3330.0
72	2706.0
73	2082.0
74	1621.5
75	889.0
76	617.0
77	499.0
78	381.0
79	261.5
80	142.0
81	115.5
82	89.0
83	70.5
84	52.0
85	37.0
86	22.0
87	14.5
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	6.0
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1207070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.333674689511703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59285827973375	22.432673314294917
2	12.24531369937274	7.673813518543332
3	4.783906144875683	4.496920766660721
4	2.370381297092947	2.9709102581285283
5	1.4033779811169333	2.1986494563370846
6	0.8701148985420839	1.6358338304049085
7	0.6271024202521983	1.3754596263231487
8	0.467529833067014	1.1719542157570824
9	0.3987567400998474	1.1245062577084908
>10	4.404530900209217	32.92868286675883
>50	0.7543025278088196	15.175501262813107
>100	0.0764946408403952	4.450556440642227
>500	0.003198382193022782	0.6161679346434712
>1k	0.002132254795348521	1.7483702509841517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	4762	0.39450901770402713	No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	4636	0.38407051786557533	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	3032	0.2511867580173478	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2855	0.2365231511014274	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2132	0.1766260448855493	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1358	0.1125038315922026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6569047362621886E-4	0.0	0.0	0.4297182433495986	0.0
2	1.6569047362621886E-4	0.0	0.0	1.415079490004722	0.0
3	1.6569047362621886E-4	0.0	0.0	1.957798636367402	0.0
4	1.6569047362621886E-4	0.0	0.0	2.5971153288541675	0.0
5	2.485357104393283E-4	0.0	0.0	3.629284134308698	0.0
6	2.485357104393283E-4	0.0	0.0	4.297679504916865	0.0
7	2.485357104393283E-4	0.0	0.0	4.866246365165235	0.0
8	2.485357104393283E-4	0.0	0.0	5.7433288873056245	0.0
9	2.485357104393283E-4	0.0	0.0	6.107930774520119	0.0
10	2.485357104393283E-4	0.0	0.0	7.040850986272544	0.0
11	2.485357104393283E-4	0.0	0.0	8.839586767958776	0.0
12	2.485357104393283E-4	0.0	0.0	10.3279842925431	0.0
13	2.485357104393283E-4	0.0	0.0	10.83392015376076	0.0
14	2.485357104393283E-4	0.0	0.0	11.004249960648513	0.0
15	2.485357104393283E-4	0.0	0.0	11.253945504403225	0.0
16	2.485357104393283E-4	0.0	0.0	11.948519969844334	0.0
17	2.485357104393283E-4	0.0	0.0	13.000240251186758	0.0
18	2.485357104393283E-4	0.0	0.0	14.197933839793881	0.0
19	2.485357104393283E-4	0.0	0.0	14.9823125419404	0.0
20	2.485357104393283E-4	0.0	0.0	15.786408410448441	0.0
21	2.485357104393283E-4	0.0	0.0	16.91442915489574	0.0
22	2.485357104393283E-4	0.0	0.0	18.126537814708342	0.0
23	3.313809472524377E-4	0.0	0.0	19.390838973713205	0.0
24	3.313809472524377E-4	0.0	0.0	20.307107292866196	0.0
25	3.313809472524377E-4	0.0	0.0	21.14061322044289	0.0
26	3.313809472524377E-4	0.0	0.0	21.877355911421873	0.0
27	3.313809472524377E-4	0.0	0.0	22.593801519381643	0.0
28	3.313809472524377E-4	0.0	0.0	23.396406173627046	0.0
29	3.313809472524377E-4	0.0	0.0	24.18774387566587	0.0
30	3.313809472524377E-4	0.0	0.0	25.01528494619202	0.0
31	4.1422618406554716E-4	0.0	0.0	25.837358231088505	0.0
32	4.1422618406554716E-4	0.0	0.0	26.62430513557623	0.0
33	4.1422618406554716E-4	0.0	0.0	27.438839503922722	0.0
34	4.1422618406554716E-4	0.0	0.0	28.16530938553688	0.0
35	4.1422618406554716E-4	0.0	0.0	28.914975933458706	0.0
36	4.1422618406554716E-4	0.0	0.0	29.67740064784975	0.0
37	4.1422618406554716E-4	0.0	0.0	30.437919921794098	0.0
38	4.1422618406554716E-4	0.0	0.0	31.226440885781273	0.0
39	4.1422618406554716E-4	0.0	0.0	31.977515802728924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAT	30	2.1655924E-6	45.000004	9
CGCGGAC	25	3.8909224E-5	45.0	4
AAGCGAT	20	7.033428E-4	45.0	30
ATCGTCC	35	1.2119926E-7	45.0	31
TACGCTA	75	0.0	45.0	44
CCCAACG	20	7.033428E-4	45.0	24
CTACGAA	20	7.033428E-4	45.0	11
AACGATC	25	3.8909224E-5	45.0	20
GTCCGAT	50	2.1827873E-11	45.0	9
TCGTACC	20	7.033428E-4	45.0	37
TTACGTT	20	7.033428E-4	45.0	1
TTCGCGC	25	3.8909224E-5	45.0	1
TCGACAA	20	7.033428E-4	45.0	19
TGCGTTA	20	7.033428E-4	45.0	12
TCGCAAT	110	0.0	42.954544	21
CAATCGG	105	0.0	42.857143	25
TGTACGC	90	0.0	42.5	42
CGTTCGG	70	0.0	41.785713	2
CGGTCTA	70	0.0	41.785713	31
CGCAATC	115	0.0	41.08696	22
>>END_MODULE