##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478554
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23662443170828	33.0	31.0	34.0	30.0	34.0
2	32.436629301330896	34.0	31.0	34.0	31.0	34.0
3	32.512913968647744	34.0	31.0	34.0	31.0	34.0
4	35.99979845173054	37.0	35.0	37.0	35.0	37.0
5	35.923342671285596	37.0	35.0	37.0	35.0	37.0
6	35.44447548077378	37.0	35.0	37.0	33.0	37.0
7	35.85920838873656	37.0	35.0	37.0	35.0	37.0
8	35.958737388015585	37.0	35.0	37.0	35.0	37.0
9	37.76417026364948	39.0	38.0	39.0	35.0	39.0
10	37.30511770283669	39.0	37.0	39.0	34.0	39.0
11	37.20137309831092	39.0	37.0	39.0	34.0	39.0
12	37.011712795068696	39.0	37.0	39.0	33.0	39.0
13	36.897634445546124	39.0	37.0	39.0	33.0	39.0
14	37.98020971841407	40.0	37.0	41.0	33.0	41.0
15	38.071248665926305	40.0	37.0	41.0	33.0	41.0
16	38.126974057085505	40.0	37.0	41.0	33.0	41.0
17	38.09702858333209	40.0	37.0	41.0	33.0	41.0
18	38.02600243210596	40.0	37.0	41.0	33.0	41.0
19	38.04708519269502	40.0	37.0	41.0	34.0	41.0
20	37.97509120397361	40.0	36.0	41.0	33.0	41.0
21	37.79954469028524	40.0	36.0	41.0	33.0	41.0
22	37.885204733814255	40.0	36.0	41.0	33.0	41.0
23	37.82062001117308	40.0	36.0	41.0	33.0	41.0
24	37.76171786759225	40.0	36.0	41.0	33.0	41.0
25	37.687418247828624	40.0	36.0	41.0	33.0	41.0
26	37.64222612092625	40.0	36.0	41.0	33.0	41.0
27	37.587896011914346	39.0	36.0	41.0	33.0	41.0
28	37.46261550136146	39.0	35.0	41.0	33.0	41.0
29	37.368505309917666	39.0	35.0	41.0	32.0	41.0
30	37.33591874223058	39.0	35.0	41.0	32.0	41.0
31	37.26430823629032	39.0	35.0	41.0	32.0	41.0
32	37.14318651872032	39.0	35.0	41.0	32.0	41.0
33	37.0788540695842	39.0	35.0	41.0	32.0	41.0
34	36.87345203489355	39.0	35.0	41.0	31.0	41.0
35	36.71589810044138	39.0	35.0	41.0	31.0	41.0
36	36.44783619671652	39.0	35.0	41.0	30.0	41.0
37	36.36591561755607	39.0	35.0	41.0	30.0	41.0
38	36.30377314592501	39.0	35.0	40.0	30.0	41.0
39	36.23744279884265	39.0	35.0	40.0	30.0	41.0
40	36.16243911280887	38.0	35.0	40.0	30.0	41.0
41	36.05143200721786	38.0	35.0	40.0	30.0	41.0
42	36.018006782302166	38.0	35.0	40.0	30.0	41.0
43	35.96651458113806	38.0	35.0	40.0	30.0	41.0
44	35.821885436717224	38.0	35.0	40.0	29.0	41.0
45	35.841152910208216	38.0	35.0	40.0	29.0	41.0
46	35.74133105723565	38.0	35.0	40.0	29.0	41.0
47	35.55156389283043	38.0	34.0	40.0	29.0	41.0
48	35.46323773091818	37.0	34.0	40.0	28.0	41.0
49	35.429254528410866	37.0	34.0	40.0	28.0	41.0
50	35.33046205955278	37.0	34.0	40.0	28.0	41.0
51	34.343754776626355	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	31.0
10	50.0
11	52.0
12	38.0
13	34.0
14	43.0
15	67.0
16	107.0
17	183.0
18	357.0
19	636.0
20	1045.0
21	1729.0
22	2684.0
23	4017.0
24	5897.0
25	8797.0
26	12133.0
27	14967.0
28	17002.0
29	20509.0
30	25728.0
31	32382.0
32	42589.0
33	60734.0
34	103762.0
35	119522.0
36	131796.0
37	191996.0
38	303909.0
39	375637.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.610427485232197	4.631687445977624	52.51969153646062	17.23819353232956
2	19.991018251616104	4.554111652330588	50.92685150491629	24.528018591137016
3	20.677837941664627	4.432776888771056	52.76012915321321	22.12925601635111
4	19.266661887222245	4.823023034667655	48.00791854744568	27.90239653066442
5	18.047700658886995	8.949284233108834	48.20723490653706	24.79578020146711
6	21.562891852445023	9.178156496144206	51.00172195266456	18.257229698746208
7	82.037653004219	2.4409659708066123	8.165816060826996	7.355564964147404
8	83.57368077188929	2.7499841060928447	9.795651697536918	3.8806834244809454
9	78.30278772368138	5.037827499029457	11.611006429254529	5.048378348034634
10	42.74865848660245	27.5555035527955	15.411949783369428	14.28388817723262
11	28.905200621688486	26.74978391049634	25.13212233033085	19.212893137484325
12	26.202695336118936	20.79234170682978	29.67277488681509	23.33218807023619
13	25.979504299470968	20.50307259660452	30.212220859028484	23.305202244896027
14	21.45176976965332	22.812761657673647	31.114047914381217	24.621420658291818
15	19.956660358701814	22.110048060469893	35.42853355372885	22.50475802709945
16	25.879812303101545	21.802856033665325	31.09274331542845	21.22458834780468
17	24.152110778503864	21.944210356875704	28.19274777924918	25.710931085371246
18	24.140139622901835	24.818775641606596	29.695364525069763	21.345720210421803
19	24.544859369356818	24.762910248797134	29.512618409608304	21.17961197223774
20	26.16177697940014	26.520844013813495	28.92129742978613	18.39608157700023
21	26.51387774812418	26.95038530888963	27.80128422769814	18.734452715288043
22	27.272389104489925	22.441385299420922	28.23312506678823	22.053100529300927
23	23.845392187231578	25.64742309039778	28.05917132549775	22.44801339687289
24	23.887933751489633	26.856983241734827	27.574305706791908	21.68077729998363
25	24.0579647412269	26.958163178348578	25.756042728233126	23.2278293521914
26	22.05654984532185	25.647355456750308	28.207221379807567	24.08887331812027
27	21.275584117996367	23.684965175434918	30.564118726810115	24.475331979758604
28	21.786894492862622	24.311658552883426	28.93766477247365	24.963782181780307
29	20.7172683581391	25.735211564812648	29.12277806559652	24.42474201145173
30	22.463569135790777	24.70095782771546	29.168363143990682	23.66710989250308
31	24.93713452467749	24.501032765796854	27.027487667004387	23.534345042521274
32	23.940890897457923	25.968141846696163	26.007234094933292	24.083733160912622
33	23.310342402103675	23.57587210206729	26.568661002574135	26.545124493254896
34	22.636914174254034	24.530453402445904	29.327234581895556	23.50539784140451
35	24.280344174105238	25.056846080697763	27.677649920124665	22.985159825072333
36	22.73133074612087	25.718506053887786	28.17029340828945	23.379869791701893
37	22.923004503048247	27.41739564466364	26.453142732696943	23.206457119591168
38	23.985393837492577	26.32998186065575	25.26184366617655	24.422780635675124
39	22.468574025703493	24.479051830369404	27.812849581415357	25.23952456251175
40	23.27767535037611	22.97156546193105	30.4602334442976	23.290525743395236
41	22.119922573000377	25.13212233033085	28.333358132337406	24.414596964331366
42	22.46931799582565	22.705224158197808	28.303328792861134	26.522129053115407
43	22.411491227239587	22.92516877976726	29.375727907130887	25.287612085862264
44	22.994155100185722	23.2269501147743	28.599226000538362	25.17966878450161
45	22.851786272263304	23.096214274216564	27.642818591678086	26.409180861842042
46	22.426438263330255	23.273346796938092	29.033095849052522	25.267119090679135
47	21.357691366023833	23.715941385975757	30.409643475990734	24.51672377200968
48	20.95080734284984	23.631737494876752	30.04624788813936	25.37120727413405
49	22.616421179070905	23.099190154705205	29.52472483250527	24.75966383371862
50	21.394281169304605	22.858279102420337	29.60689971418021	26.140540014094853
51	21.696671207138866	23.37046871470369	27.65289600515098	27.279964073006465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	356.0
1	1195.0
2	2034.0
3	5061.0
4	8088.0
5	5504.5
6	2921.0
7	2958.5
8	2996.0
9	3167.0
10	3338.0
11	3385.0
12	3432.0
13	3392.0
14	3352.0
15	3379.0
16	3406.0
17	3226.5
18	3047.0
19	3369.5
20	3692.0
21	3809.5
22	3927.0
23	4675.5
24	5424.0
25	7092.5
26	9838.5
27	10916.0
28	12752.0
29	14588.0
30	24249.0
31	33910.0
32	32469.5
33	31029.0
34	33392.0
35	35755.0
36	39709.0
37	43663.0
38	51086.5
39	58510.0
40	59903.5
41	61297.0
42	65612.0
43	69927.0
44	76949.0
45	83971.0
46	95461.0
47	106951.0
48	120200.0
49	133449.0
50	133667.0
51	133885.0
52	119405.5
53	104926.0
54	95543.5
55	86161.0
56	83766.5
57	81372.0
58	76597.5
59	71823.0
60	68317.5
61	64812.0
62	59788.5
63	54765.0
64	52372.0
65	49979.0
66	41930.5
67	33882.0
68	27561.0
69	21240.0
70	17726.0
71	14212.0
72	11889.0
73	9566.0
74	8003.0
75	4956.0
76	3472.0
77	2613.5
78	1755.0
79	1335.0
80	915.0
81	650.0
82	385.0
83	272.5
84	160.0
85	100.5
86	41.0
87	26.5
88	12.0
89	9.5
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1478554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.327876797310488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0402788481864	18.499551839080684
2	10.703494112378111	5.421935603581019
3	4.164773955998048	3.164546449384979
4	1.957109264342226	1.9827768930453946
5	1.2445985955096928	1.5761519944587583
6	0.7598968647063851	1.1547944500767504
7	0.5703819997516718	1.011259551197975
8	0.43670361541813124	0.8848620294600391
9	0.3421905276918452	0.7800263573927148
>10	5.370027354062684	35.734657983287846
>50	1.305161908401295	21.12134600524583
>100	0.09673614711871276	4.345885394824702
>500	0.004323403217405883	0.7277818149962874
>1k	0.003782977815230148	2.2556856159923484
>5k	2.702127010878677E-4	0.49679971742129486
>10k+	2.702127010878677E-4	0.8419383005533503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	12302	0.832029131164638	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	7259	0.490952646978061	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4819	0.32592654715350267	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG	3280	0.2218383636985866	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	3006	0.20330674429205833	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	2901	0.1962052113078048	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTCG	2835	0.19174139057484543	No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	2655	0.17956733403041078	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2185	0.1477795197199426	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	2040	0.13797264083692581	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1933	0.1307358405577341	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	1805	0.12207873368169171	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1794	0.12133476355953182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0290094240724382E-4	0.0	0.0	0.19383803364638694	0.0
2	2.0290094240724382E-4	0.0	0.0	0.7311197291407686	0.0
3	2.0290094240724382E-4	0.0	0.0	1.1021579191561486	0.0
4	2.0290094240724382E-4	0.0	0.0	1.5165492771992095	0.0
5	2.705345898763251E-4	0.0	0.0	2.3347135106326857	0.0
6	2.705345898763251E-4	0.0	0.0	2.8747681856733	0.0
7	2.705345898763251E-4	0.0	0.0	3.3582811314297616	0.0
8	2.705345898763251E-4	0.0	0.0	4.226967699522642	0.0
9	2.705345898763251E-4	0.0	0.0	4.658605637670318	0.0
10	2.705345898763251E-4	0.0	0.0	5.392768880947195	0.0
11	2.705345898763251E-4	0.0	0.0	6.708784393400579	0.0
12	2.705345898763251E-4	0.0	0.0	7.843609364284294	0.0
13	2.705345898763251E-4	0.0	0.0	8.480041986968349	0.0
14	2.705345898763251E-4	0.0	0.0	8.627348071156009	0.0
15	2.705345898763251E-4	0.0	0.0	9.062300058029669	0.0
16	2.705345898763251E-4	0.0	0.0	9.538238035269595	0.0
17	2.705345898763251E-4	0.0	0.0	10.193269911007647	0.0
18	2.705345898763251E-4	0.0	0.0	10.872176464302285	0.0
19	2.705345898763251E-4	0.0	0.0	11.345679630233322	0.0
20	2.705345898763251E-4	0.0	0.0	11.946401687053703	0.0
21	2.705345898763251E-4	0.0	0.0	12.67386919923114	0.0
22	2.705345898763251E-4	0.0	0.0	13.442863770954595	0.0
23	2.705345898763251E-4	0.0	0.0	14.177229915173879	0.0
24	2.705345898763251E-4	0.0	0.0	14.738792090109662	0.0
25	2.705345898763251E-4	0.0	0.0	15.439409044241874	0.0
26	2.705345898763251E-4	0.0	0.0	15.870911715094612	0.0
27	2.705345898763251E-4	0.0	0.0	16.345767553975033	0.0
28	3.3816823734540637E-4	0.0	0.0	16.79958932849257	0.0
29	3.3816823734540637E-4	0.0	0.0	17.31326688102024	0.0
30	3.3816823734540637E-4	0.0	0.0	17.859273317038134	0.0
31	4.0580188481448764E-4	0.0	0.0	18.338254808414167	0.0
32	4.0580188481448764E-4	0.0	0.0	18.9012372899468	0.0
33	4.0580188481448764E-4	0.0	0.0	19.379204276610796	0.0
34	4.0580188481448764E-4	0.0	0.0	20.032207142924776	0.0
35	4.0580188481448764E-4	0.0	0.0	20.5464257646322	0.0
36	4.0580188481448764E-4	0.0	0.0	21.016411980894848	0.0
37	4.0580188481448764E-4	0.0	0.0	21.52122952560407	0.0
38	4.0580188481448764E-4	0.0	0.0	22.064260081133323	0.0
39	4.0580188481448764E-4	0.0	0.0	22.826220753519994	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	35	1.2122473E-7	45.0	1
TCAATCG	25	3.89135E-5	45.0	32
CGAATCG	20	7.0339424E-4	45.0	11
CGACTTA	20	7.0339424E-4	45.0	20
CTCAACT	4200	0.0	42.696426	2
TATGCGG	90	0.0	42.5	2
ACTTGAC	4080	0.0	42.463234	6
CTTGACC	4340	0.0	42.35599	7
TTGACCC	3845	0.0	42.249672	8
GCATTAG	3480	0.0	42.155174	16
TCAACTT	4135	0.0	42.06167	3
CCATACG	75	0.0	42.0	2
TGCATTA	3495	0.0	41.97425	15
CATTAGA	3450	0.0	41.869568	17
GCTCAAC	4525	0.0	41.618786	1
CCTGCAT	3570	0.0	41.596638	13
TACGCGG	120	0.0	41.250004	2
CGTTTTT	2400	0.0	40.96875	1
CAACTTG	4255	0.0	40.7168	4
TGACCCT	3675	0.0	40.591835	9
>>END_MODULE