FastQCFastQC Report
Sat 14 Jan 2017
SRR2934331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193558
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272361.2416357351845722No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC137970.628978125948801No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG87670.3996703073271826No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC85570.3900968198698188No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT34180.1558199053774735No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC26810.12222152320567772No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25440.11597596234063563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT147400.043.7177771
TAACGCC556.184564E-1140.9090912
TTTCGCG7400.038.006761
TTTGGGC52550.037.7640344
TTCTTCG6200.037.3790361
GTTTTTT180000.037.28752
GGGCGAT30400.037.1546067
TTTGGGA81250.037.084
TACGGCT13500.037.07
TCGTTTG8100.036.9444431
TTGGGAC34900.036.941265
TTGGGAT41850.036.9354825
TTTGTCG7250.036.9310381
CCGATGA11850.036.83544518
TGGGCGA21850.036.659046
TTGGGTA17250.036.1304325
CGTTCAT2750.036.017
CGATGAA12250.035.81632619
ACCACCG16000.035.7187514
TCGTTCA2900.035.68965516
Produced by FastQC (version 0.11.5)