##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934331.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2193558
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17584855289899	33.0	31.0	34.0	30.0	34.0
2	32.383472422429676	34.0	31.0	34.0	30.0	34.0
3	32.40290979313061	34.0	31.0	34.0	30.0	34.0
4	35.96008448374741	37.0	35.0	37.0	35.0	37.0
5	35.903122233376095	37.0	35.0	37.0	35.0	37.0
6	35.48160249238908	37.0	35.0	37.0	33.0	37.0
7	35.81399671219088	37.0	35.0	37.0	35.0	37.0
8	35.85870854565961	37.0	35.0	37.0	35.0	37.0
9	37.6869957393422	39.0	38.0	39.0	35.0	39.0
10	37.2736362567117	39.0	37.0	39.0	34.0	39.0
11	37.16740017815804	39.0	37.0	39.0	34.0	39.0
12	36.91225944333362	39.0	37.0	39.0	33.0	39.0
13	36.790453227131444	39.0	35.0	39.0	33.0	39.0
14	37.78302055382169	40.0	37.0	41.0	33.0	41.0
15	37.863074967700875	40.0	37.0	41.0	33.0	41.0
16	37.98011404302964	40.0	37.0	41.0	33.0	41.0
17	37.91392750955297	40.0	37.0	41.0	33.0	41.0
18	37.80435210739812	39.0	36.0	41.0	33.0	41.0
19	37.73990840451905	39.0	36.0	41.0	33.0	41.0
20	37.5054304467901	39.0	35.0	41.0	33.0	41.0
21	37.383866758936854	39.0	35.0	41.0	33.0	41.0
22	37.504477200967564	39.0	35.0	41.0	33.0	41.0
23	37.44162224112606	39.0	35.0	41.0	33.0	41.0
24	37.37564632437346	39.0	35.0	41.0	33.0	41.0
25	37.31175332496337	39.0	35.0	41.0	33.0	41.0
26	37.251628176688286	39.0	35.0	41.0	33.0	41.0
27	37.15333535744211	39.0	35.0	41.0	32.0	41.0
28	37.00115793610198	39.0	35.0	41.0	32.0	41.0
29	36.875417472435196	39.0	35.0	41.0	31.0	41.0
30	36.773853255760734	39.0	35.0	41.0	31.0	41.0
31	36.625816595686096	39.0	35.0	41.0	31.0	41.0
32	36.33954242376996	39.0	35.0	41.0	30.0	41.0
33	36.11676691475676	39.0	35.0	41.0	30.0	41.0
34	35.750611563496385	39.0	35.0	41.0	27.0	41.0
35	35.55141418644959	38.0	35.0	40.0	26.0	41.0
36	35.36836363570054	38.0	35.0	40.0	25.0	41.0
37	35.28368112445625	38.0	34.0	40.0	25.0	41.0
38	35.184495691474766	38.0	34.0	40.0	25.0	41.0
39	35.07668226689242	38.0	34.0	40.0	24.0	41.0
40	34.908961604844734	38.0	34.0	40.0	23.0	41.0
41	34.74821363282849	38.0	34.0	40.0	23.0	41.0
42	34.71391410667053	38.0	34.0	40.0	23.0	41.0
43	34.674968247933265	38.0	34.0	40.0	23.0	41.0
44	34.558255127058416	38.0	34.0	40.0	23.0	41.0
45	34.60473486454427	37.0	34.0	40.0	23.0	41.0
46	34.56347404536374	37.0	34.0	40.0	23.0	41.0
47	34.327074096057636	37.0	33.0	40.0	23.0	41.0
48	34.23219217362841	37.0	33.0	40.0	23.0	41.0
49	34.23327671299323	37.0	33.0	40.0	23.0	41.0
50	34.10091914597198	37.0	33.0	40.0	23.0	41.0
51	33.20867877667242	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	37.0
10	45.0
11	57.0
12	43.0
13	51.0
14	85.0
15	118.0
16	200.0
17	416.0
18	911.0
19	1744.0
20	3080.0
21	5094.0
22	7843.0
23	11667.0
24	17157.0
25	26195.0
26	35708.0
27	40202.0
28	40904.0
29	41696.0
30	46109.0
31	55392.0
32	69607.0
33	95888.0
34	154246.0
35	166241.0
36	179030.0
37	258513.0
38	411349.0
39	523759.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.727979839147174	4.1966521970241955	51.55286525361992	20.522502710208713
2	23.66757569209476	7.001501669889741	48.48898456297942	20.841938075036083
3	18.85530266352656	7.01235162234142	52.37016755426572	21.7621781598663
4	17.6833710346387	4.42728206867564	53.77806285495984	24.111284041725817
5	19.14743079508269	5.458939312295366	51.62211347956151	23.771516413060425
6	20.318313899153793	7.986659117287987	53.905299062071755	17.789727921486463
7	78.44807385991162	2.1356171115603053	15.196406933393144	4.219902095134936
8	80.35406403660173	4.256646051757008	11.84573191135133	3.5435580002899396
9	76.01932568001392	3.989910455980649	13.196414227478826	6.794349636526594
10	40.80457412113106	22.00999472090549	21.708293101891996	15.47713805607146
11	30.060522675944746	22.860120407119393	28.019865442354387	19.05949147458148
12	26.61370248700969	19.641559511989197	32.50768842218898	21.23704957881214
13	26.043806455083473	18.345400486333162	35.360177392163784	20.25061566641958
14	21.12572359609365	21.484774963780307	35.62075860314612	21.76874283697992
15	19.156457226113922	22.125605978962035	37.62471746814992	21.093219326774125
16	24.098473803747154	22.395259209011115	32.6386172601773	20.867649727064432
17	24.273349507968334	23.065585683168624	32.02554935862193	20.635515450241115
18	23.8256749992478	22.49828816926655	33.23545582109067	20.440581010394983
19	24.021657963910688	24.292040602527948	30.852250088668733	20.834051344892636
20	24.306127305500926	27.022308049297077	30.678194969086753	17.993369676115243
21	24.70912553942043	24.81370449288325	32.83847520785865	17.63869475983767
22	23.068229789228276	21.10748838188915	34.44135053643441	21.38293129244816
23	22.84621605628846	22.21258795071751	33.62044678098323	21.320749212010806
24	22.582990739246465	24.2144953541233	31.69836402775764	21.50414987887259
25	23.341803590331324	25.20598953845761	29.691761056694194	21.76044581451687
26	21.203360020569324	24.0285873453084	30.861550047913028	23.906502586209257
27	21.93609651534174	23.222454113362858	32.56918668209366	22.272262689201742
28	20.33477117997336	24.257211343397348	31.591733612696814	23.816283863932476
29	22.315161030617837	24.25976427338598	30.354884621240924	23.070190074755264
30	23.328036003606925	24.078916536512825	31.232910185187716	21.36013727469253
31	24.030547630835382	23.174176383756436	30.230429284295194	22.564846701112987
32	25.73412692985551	23.59864658240174	28.17677946058413	22.490447027158616
33	26.399484308142295	23.115003113662823	27.953033382294883	22.53247919589999
34	22.901423167292588	23.67509771795412	30.561945478533048	22.86153363622024
35	22.76857963181279	26.574405600398986	28.36692715670158	22.290087611086644
36	25.591117262456702	25.901662960359378	26.886182175260465	21.62103760192345
37	23.578679022847812	26.504017673569606	27.883466040104704	22.033837263477874
38	25.18415286944772	25.074012175652527	27.421066595914034	22.32076835898572
39	24.052749004129364	23.651300763417243	28.428744532854843	23.86720569959855
40	24.496958822151043	23.879742409364148	29.501294244328164	22.122004524156644
41	22.06232978567241	24.56479381899179	29.435328356943373	23.93754803839242
42	22.070307691886878	24.719200495268417	30.17713687078254	23.033354942062164
43	22.93880535641182	23.357896166866798	29.810426713129996	23.89287176359139
44	23.1096237254725	23.267495092447977	29.39730793532699	24.225573246752536
45	22.327834504489967	23.036637280618976	28.73869758629587	25.896830628595186
46	22.937939183737107	23.721415162033555	29.24714094635291	24.093504707876427
47	21.051323922139282	24.3189831315151	31.351347901445966	23.278345044899655
48	21.48358967485701	25.28189361758385	29.74938433358042	23.485132373978715
49	22.97481990446571	23.150379429219562	30.70919483323441	23.16560583308032
50	21.75137379545013	23.32698747879017	29.978600976131016	24.943037749628687
51	20.982121284233195	23.939781852132473	29.36029045049185	25.717806413142487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	1484.0
2	2568.0
3	23719.0
4	44870.0
5	29965.0
6	15060.0
7	14686.0
8	14312.0
9	14218.0
10	14124.0
11	13735.0
12	13346.0
13	12761.0
14	12176.0
15	11556.5
16	10937.0
17	10523.5
18	10110.0
19	9760.0
20	9410.0
21	9453.5
22	9497.0
23	10400.5
24	11304.0
25	12697.0
26	16684.5
27	19279.0
28	22649.5
29	26020.0
30	29611.0
31	33202.0
32	38693.5
33	44185.0
34	50081.5
35	55978.0
36	57863.0
37	59748.0
38	65178.0
39	70608.0
40	75257.5
41	79907.0
42	84661.5
43	89416.0
44	94746.5
45	100077.0
46	112288.5
47	124500.0
48	159760.5
49	195021.0
50	188873.5
51	182726.0
52	180031.0
53	177336.0
54	164311.0
55	151286.0
56	142753.5
57	134221.0
58	123219.5
59	112218.0
60	103166.0
61	94114.0
62	85694.5
63	77275.0
64	69063.5
65	60852.0
66	52114.0
67	43376.0
68	36474.0
69	29572.0
70	24401.5
71	19231.0
72	16295.0
73	13359.0
74	10769.0
75	6388.5
76	4598.0
77	3654.0
78	2710.0
79	1882.5
80	1055.0
81	865.0
82	675.0
83	518.5
84	362.0
85	264.5
86	167.0
87	100.0
88	33.0
89	39.0
90	45.0
91	32.5
92	20.0
93	11.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2193558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.672924177703877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97778401936846	21.025274563579448
2	8.09896311019782	4.482439841330501
3	2.970916895197804	2.466418739372048
4	1.5827874433705555	1.7520142763926063
5	1.0143697458872143	1.4035288533046817
6	0.7622920156055539	1.2656909489472934
7	0.5913239883128539	1.1454564725127343
8	0.5031590275034801	1.113910529394482
9	0.467194260788096	1.1635768219542655
>10	7.602516619288668	50.140011836435974
>50	0.3582013479977082	6.054935407266434
>100	0.0628078184276131	3.0847264999658557
>500	0.003841854027096283	0.7225282627877453
>1k	0.0031737055006447554	1.481989548540586
>5k	3.3407426322576374E-4	0.8007855943020216
>10k+	3.3407426322576374E-4	1.8967118039133486
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27236	1.2416357351845722	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	13797	0.628978125948801	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	8767	0.3996703073271826	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC	8557	0.3900968198698188	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	3418	0.1558199053774735	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	2681	0.12222152320567772	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2544	0.11597596234063563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.19116248578793E-4	0.0	0.0	0.10170690722561246	0.0
2	3.19116248578793E-4	0.0	0.0	0.3825747940104615	0.0
3	3.19116248578793E-4	0.0	0.0	0.5889974188054293	0.0
4	3.19116248578793E-4	0.0	0.0	1.0186190654635072	0.0
5	3.19116248578793E-4	0.0	0.0	2.2485386755216865	0.0
6	3.19116248578793E-4	0.0	0.0	3.098801125842125	0.0
7	3.19116248578793E-4	0.0	0.0	3.84370962609605	0.0
8	3.19116248578793E-4	0.0	0.0	4.828912661529807	0.0
9	3.19116248578793E-4	0.0	0.0	5.513599366873363	0.0
10	3.19116248578793E-4	0.0	0.0	6.791568766360406	0.0
11	3.19116248578793E-4	0.0	0.0	7.779826200173417	0.0
12	3.19116248578793E-4	0.0	0.0	8.898647767690665	0.0
13	3.19116248578793E-4	0.0	0.0	9.193465593341958	0.0
14	3.19116248578793E-4	0.0	0.0	9.396514703509093	0.0
15	3.19116248578793E-4	0.0	0.0	9.606082902754338	0.0
16	3.19116248578793E-4	0.0	0.0	9.847927431141551	0.0
17	3.19116248578793E-4	0.0	0.0	10.154506969954749	0.0
18	3.19116248578793E-4	0.0	0.0	10.502799561260746	0.0
19	3.19116248578793E-4	0.0	0.0	10.850955388460209	0.0
20	3.19116248578793E-4	0.0	0.0	11.157945219593008	0.0
21	3.19116248578793E-4	0.0	0.0	11.529533297045257	0.0
22	3.19116248578793E-4	0.0	0.0	11.947438818576941	0.0
23	3.19116248578793E-4	0.0	0.0	12.329375380090246	0.0
24	4.1029231960130525E-4	0.0	0.0	12.64890192098864	0.0
25	4.1029231960130525E-4	0.0	0.0	12.926077176897078	0.0
26	5.014683906238176E-4	0.0	0.0	13.187706912696177	0.0
27	5.014683906238176E-4	0.0	0.0	13.479151223719636	0.0
28	5.014683906238176E-4	0.0	0.0	13.749488274301386	0.0
29	5.014683906238176E-4	0.0	0.0	14.075442728206868	0.0
30	5.470564261350737E-4	0.0	0.0	14.47730126123859	0.0
31	5.926444616463298E-4	0.0	0.0	14.786023437720816	0.0
32	6.38232497157586E-4	0.0	0.0	15.105322038441654	0.0
33	6.38232497157586E-4	0.0	0.0	15.416460380805978	0.0
34	6.838205326688421E-4	0.0	0.0	15.751441265742688	0.0
35	6.838205326688421E-4	0.0	0.0	16.129548432273047	0.0
36	6.838205326688421E-4	0.0	0.0	16.470638113968267	0.0
37	6.838205326688421E-4	0.0	0.0	16.821620399369426	0.0
38	6.838205326688421E-4	0.0	0.0	17.243218551777524	0.0
39	6.838205326688421E-4	0.0	0.0	17.975909458514433	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14740	0.0	43.717777	1
TAACGCC	55	6.184564E-11	40.90909	12
TTTCGCG	740	0.0	38.00676	1
TTTGGGC	5255	0.0	37.764034	4
TTCTTCG	620	0.0	37.379036	1
GTTTTTT	18000	0.0	37.2875	2
GGGCGAT	3040	0.0	37.154606	7
TTTGGGA	8125	0.0	37.08	4
TACGGCT	1350	0.0	37.0	7
TCGTTTG	810	0.0	36.944443	1
TTGGGAC	3490	0.0	36.94126	5
TTGGGAT	4185	0.0	36.935482	5
TTTGTCG	725	0.0	36.931038	1
CCGATGA	1185	0.0	36.835445	18
TGGGCGA	2185	0.0	36.65904	6
TTGGGTA	1725	0.0	36.130432	5
CGTTCAT	275	0.0	36.0	17
CGATGAA	1225	0.0	35.816326	19
ACCACCG	1600	0.0	35.71875	14
TCGTTCA	290	0.0	35.689655	16
>>END_MODULE