SRR2934330.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1253517
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4699	0.3748652790508625	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	3881	0.30960888444273194	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3500	0.27921440235752687	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3454	0.27554472735511365	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3265	0.2604671496278072	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1895	0.1511746549907181	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1255	0.10011830713105606	TruSeq Adapter, Index 23 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	25	3.8910075E-5	45.000004	1
GTTCGCT	25	3.8910075E-5	45.000004	32
TTACACG	140	0.0	45.000004	34
TAATGCG	25	3.8910075E-5	45.000004	24
TCGCAAG	20	7.0335314E-4	45.0	14
ACCGGCC	20	7.0335314E-4	45.0	27
AGATTCG	20	7.0335314E-4	45.0	33
GGACGTA	20	7.0335314E-4	45.0	8
GCGATAT	45	3.8562575E-10	45.0	9
CGTTTCA	20	7.0335314E-4	45.0	17
CGATAAG	20	7.0335314E-4	45.0	20
ATACCGG	20	7.0335314E-4	45.0	11
ACGGTAC	20	7.0335314E-4	45.0	12
AAGATCG	30	2.1656597E-6	44.999996	28
GGCGATA	60	0.0	44.999996	8
CGAACGA	110	0.0	42.954544	22
TCGTTCA	240	0.0	42.187496	16
TCGTTTG	350	0.0	41.785713	1
AACGACA	60	3.6379788E-12	41.249996	10
TAACGAA	115	0.0	41.086956	19