##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1253517
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16266871530262	33.0	31.0	34.0	30.0	34.0
2	32.349849264110496	34.0	31.0	34.0	30.0	34.0
3	32.41170083852074	34.0	31.0	34.0	30.0	34.0
4	35.910127265924594	37.0	35.0	37.0	35.0	37.0
5	35.87489359936882	37.0	35.0	37.0	35.0	37.0
6	35.51869180872696	37.0	35.0	37.0	33.0	37.0
7	35.88335698678199	37.0	35.0	37.0	35.0	37.0
8	35.97905333553514	37.0	35.0	37.0	35.0	37.0
9	37.77180843977385	39.0	38.0	39.0	35.0	39.0
10	37.19015059229352	39.0	37.0	39.0	34.0	39.0
11	37.083576050424526	39.0	37.0	39.0	33.0	39.0
12	36.98920078467225	39.0	37.0	39.0	33.0	39.0
13	36.949987914005156	39.0	37.0	39.0	33.0	39.0
14	38.1307848238197	40.0	37.0	41.0	33.0	41.0
15	38.159947571512795	40.0	37.0	41.0	33.0	41.0
16	38.187407111351504	40.0	37.0	41.0	33.0	41.0
17	38.11700998071825	40.0	37.0	41.0	33.0	41.0
18	38.092434326778175	40.0	37.0	41.0	33.0	41.0
19	38.11164667092668	40.0	37.0	41.0	34.0	41.0
20	38.06328514092748	40.0	37.0	41.0	34.0	41.0
21	37.90620789347093	40.0	37.0	41.0	33.0	41.0
22	37.98888726678617	40.0	37.0	41.0	33.0	41.0
23	37.94146070615716	40.0	37.0	41.0	33.0	41.0
24	37.90703037932473	40.0	37.0	41.0	33.0	41.0
25	37.82992332772511	40.0	36.0	41.0	33.0	41.0
26	37.785561743478546	40.0	36.0	41.0	33.0	41.0
27	37.71091337413055	40.0	36.0	41.0	33.0	41.0
28	37.56268004342981	40.0	36.0	41.0	33.0	41.0
29	37.48394716625303	39.0	36.0	41.0	32.0	41.0
30	37.47330111996886	39.0	36.0	41.0	32.0	41.0
31	37.42070031758644	39.0	36.0	41.0	32.0	41.0
32	37.30273542361213	39.0	36.0	41.0	32.0	41.0
33	37.26337815921124	39.0	36.0	41.0	32.0	41.0
34	37.14225654697942	39.0	35.0	41.0	31.0	41.0
35	37.108666256620374	39.0	35.0	41.0	31.0	41.0
36	36.9571717017001	39.0	35.0	41.0	31.0	41.0
37	36.8939112911911	39.0	35.0	41.0	31.0	41.0
38	36.81542492044384	39.0	35.0	41.0	31.0	41.0
39	36.804081635909206	39.0	35.0	41.0	31.0	41.0
40	36.70654087658963	39.0	35.0	41.0	30.0	41.0
41	36.61056451567869	39.0	35.0	41.0	30.0	41.0
42	36.55935260550914	39.0	35.0	41.0	30.0	41.0
43	36.508332156644066	39.0	35.0	40.0	30.0	41.0
44	36.356982793213014	39.0	35.0	40.0	30.0	41.0
45	36.39816292878358	39.0	35.0	40.0	30.0	41.0
46	36.291057879550095	39.0	35.0	40.0	30.0	41.0
47	36.06666443295145	38.0	35.0	40.0	29.0	41.0
48	35.96432118591132	38.0	35.0	40.0	29.0	41.0
49	35.98000745103577	38.0	35.0	40.0	29.0	41.0
50	35.89186823952128	38.0	35.0	40.0	29.0	41.0
51	35.010274292251324	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	16.0
10	29.0
11	18.0
12	22.0
13	15.0
14	24.0
15	23.0
16	69.0
17	126.0
18	192.0
19	418.0
20	677.0
21	1186.0
22	1912.0
23	2950.0
24	4404.0
25	6462.0
26	9335.0
27	11585.0
28	13526.0
29	16399.0
30	20671.0
31	26232.0
32	34060.0
33	45968.0
34	74092.0
35	95106.0
36	104440.0
37	159479.0
38	272677.0
39	351292.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.22433600820731	4.366354824067005	57.23448505285529	15.1748241148704
2	20.395415459064374	4.390287487126222	53.818576054413306	21.395720999396097
3	20.660669141304027	4.3346041577417775	52.19626060117254	22.808466099781654
4	18.873617190672324	4.530612668196762	52.323263266473454	24.272506874657463
5	17.899478028618677	5.515601304170586	52.23279780010961	24.352122867101127
6	20.869441738723925	6.445145937390557	54.12291975298301	18.56249257090251
7	84.3058370967446	2.0550180013513977	9.311800318623522	4.327344583280482
8	86.32934375840136	2.5189925625260767	6.809799946869488	4.341863732203073
9	81.47978846716877	4.740262796595499	8.914119234122872	4.865829502112855
10	36.44761100168566	33.399866136637954	16.66120204193481	13.491320819741576
11	24.97054288055128	26.830988331231246	28.83997584396542	19.358492944252053
12	23.938486673894328	23.113527778243135	32.62947371276177	20.31851183510076
13	23.024019618401663	23.927557424430624	33.24797350175546	19.800449455412252
14	19.401013308953928	26.881725576916786	31.481423865811152	22.235837248318134
15	17.44994284082306	28.049479983119497	33.775688722211186	20.72488845384626
16	20.165023689347652	25.423747743349313	32.68986379921453	21.721364768088506
17	19.823823689666753	26.589747087594347	29.58045243901758	24.005976783721323
18	20.387597455798364	25.81249396697452	31.66083906321175	22.139069514015368
19	22.35167133752474	27.822917439492244	29.315438083408523	20.509973139574495
20	24.470031120439533	27.450924080008487	29.565295085746747	18.51374971380524
21	23.865970704824903	27.64230560893869	29.570799598250364	18.920924087986045
22	22.47404702130087	24.93408545715774	28.904274932051184	23.68759258949021
23	20.64575111466378	27.114031959678247	28.876752369533083	23.36346455612489
24	21.458105474437122	25.88636612028397	30.507763357018693	22.147765048260215
25	21.191256281326858	26.655322584376602	28.377038364856638	23.776382769439905
26	19.789360654861483	28.421951995864436	28.329093263194675	23.45959408607941
27	20.460113424867792	27.70859908561272	28.839497190704233	22.991790298815253
28	18.592567950813592	26.553050337570212	31.991030037885405	22.86335167373079
29	19.36367835458155	24.638118190658762	31.948908550901184	24.049294903858502
30	21.221730538955594	25.253426957911223	30.789530576769202	22.735311926363984
31	20.661227570108743	27.53428952299809	29.224414188239965	22.580068718653198
32	20.84790234197063	28.05985080377849	28.415171074664325	22.677075779586556
33	20.871755229486315	26.51810864950376	28.275484097942034	24.334652023067896
34	18.20342284947073	26.22230093409184	30.668351526146036	24.905924690291396
35	19.1758069495667	25.309269838382725	29.306822324707205	26.20810088734337
36	20.482370801512864	26.419506077699783	28.307872968615506	24.79025015217185
37	19.541817143285652	27.426113885970434	30.74964280500384	22.282426165740073
38	18.931454459732098	27.98151122003132	29.613080636321648	23.473953683914935
39	19.32091866324908	24.96408106152529	30.359221294964488	25.35577898026114
40	20.800914546831038	24.716537549949464	30.361215683552757	24.121332219666748
41	17.91399717754127	26.823888307857015	29.575506355318677	25.686608159283043
42	19.50097206499792	27.121690411857198	27.46352861588634	25.91380890725854
43	20.9405217480098	24.924273065303463	28.929404228263355	25.20580095842338
44	19.596303839517134	24.470669324787778	29.95619524904728	25.97683158664781
45	20.74818291255723	24.150131190881336	28.46630719806752	26.635378698493916
46	20.536538395570222	25.13073217196097	29.54926020149707	24.78346923097174
47	18.3529222180473	25.778669136517497	31.011785241045793	24.85662340438941
48	18.523721656746577	24.700343114612725	31.282543435789062	25.493391792851632
49	19.362481721428587	23.56625398777998	31.99605589712784	25.07520839366359
50	19.226065541991055	23.07108718908479	30.47673067058524	27.226116598338912
51	17.505945272381627	23.766410826498564	29.331233641027605	29.396410260092203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	902.0
2	1549.0
3	3736.0
4	5923.0
5	4149.5
6	2376.0
7	2351.5
8	2327.0
9	2451.0
10	2575.0
11	2588.0
12	2601.0
13	2671.5
14	2742.0
15	2904.0
16	3066.0
17	3193.5
18	3321.0
19	3929.0
20	4537.0
21	4572.0
22	4607.0
23	6257.5
24	7908.0
25	9402.5
26	12753.5
27	14610.0
28	17299.5
29	19989.0
30	23506.0
31	27023.0
32	29920.0
33	32817.0
34	36615.0
35	40413.0
36	44454.0
37	48495.0
38	51708.5
39	54922.0
40	61598.0
41	68274.0
42	75482.5
43	82691.0
44	89169.0
45	95647.0
46	105848.0
47	116049.0
48	125674.5
49	135300.0
50	125846.5
51	116393.0
52	99879.0
53	83365.0
54	71502.5
55	59640.0
56	55271.0
57	50902.0
58	46614.0
59	42326.0
60	38225.0
61	34124.0
62	30317.5
63	26511.0
64	21691.5
65	16872.0
66	14062.0
67	11252.0
68	9420.5
69	7589.0
70	6542.0
71	5495.0
72	4552.5
73	3610.0
74	2915.0
75	1665.5
76	1111.0
77	859.5
78	608.0
79	422.5
80	237.0
81	199.5
82	162.0
83	139.0
84	116.0
85	86.5
86	57.0
87	33.0
88	9.0
89	5.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1253517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.58221121979127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59913084648221	20.750436922274183
2	11.886844199221613	6.795045832778055
3	4.702942708710896	4.032615055648563
4	2.3426804637037657	2.6783595133623836
5	1.3149226221452104	1.8791698061918094
6	0.8679159630203971	1.4884174425646524
7	0.56787354840153	1.1361757194581423
8	0.4279959665150811	0.9786456892922207
9	0.31670615044384237	0.8146945877933606
>10	3.5084437899914565	25.10719275346999
>50	1.33648146314545	25.785989100455463
>100	0.12072831515007919	5.760784941431112
>500	0.004513208042169028	0.7622943979262399
>1k	0.0028207550263556424	2.0301782373538493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4699	0.3748652790508625	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	3881	0.30960888444273194	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3500	0.27921440235752687	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3454	0.27554472735511365	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3265	0.2604671496278072	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1895	0.1511746549907181	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1255	0.10011830713105606	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5955108706144393E-4	0.0	0.0	0.33473818065490935	0.0
2	1.5955108706144393E-4	0.0	0.0	1.3829090471050651	0.0
3	2.3932663059216588E-4	0.0	0.0	2.0231077839391087	0.0
4	2.3932663059216588E-4	0.0	0.0	2.775630486064409	0.0
5	2.3932663059216588E-4	0.0	0.0	3.9287859678009953	0.0
6	2.3932663059216588E-4	0.0	0.0	4.741140327574337	0.0
7	2.3932663059216588E-4	0.0	0.0	5.413009955190077	0.0
8	2.3932663059216588E-4	0.0	0.0	6.435891974340994	0.0
9	2.3932663059216588E-4	0.0	0.0	6.917018277374778	0.0
10	2.3932663059216588E-4	0.0	0.0	7.918600226402993	0.0
11	2.3932663059216588E-4	0.0	0.0	9.739556783035251	0.0
12	3.1910217412288786E-4	0.0	0.0	11.194263819317968	0.0
13	3.988777176536098E-4	0.0	0.0	11.676108102243528	0.0
14	3.988777176536098E-4	0.0	0.0	11.843477192570981	0.0
15	3.988777176536098E-4	0.0	0.0	12.089185866645606	0.0
16	3.988777176536098E-4	0.0	0.0	12.758343125781302	0.0
17	3.988777176536098E-4	0.0	0.0	13.787288086240554	0.0
18	3.988777176536098E-4	0.0	0.0	14.878378195110237	0.0
19	3.988777176536098E-4	0.0	0.0	15.623960424948365	0.0
20	3.988777176536098E-4	0.0	0.0	16.32606498356225	0.0
21	3.988777176536098E-4	0.0	0.0	17.385324650563174	0.0
22	3.988777176536098E-4	0.0	0.0	18.547414993175202	0.0
23	3.988777176536098E-4	0.0	0.0	19.72514134231925	0.0
24	3.988777176536098E-4	0.0	0.0	20.586158783646333	0.0
25	3.988777176536098E-4	0.0	0.0	21.31953535532426	0.0
26	3.988777176536098E-4	0.0	0.0	21.97066334162201	0.0
27	3.988777176536098E-4	0.0	0.0	22.591237294747497	0.0
28	3.988777176536098E-4	0.0	0.0	23.287917116401292	0.0
29	3.988777176536098E-4	0.0	0.0	24.055118518536247	0.0
30	3.988777176536098E-4	0.0	0.0	24.836998620680852	0.0
31	4.7865326118433176E-4	0.0	0.0	25.58282017714957	0.0
32	4.7865326118433176E-4	0.0	0.0	26.258997684115972	0.0
33	4.7865326118433176E-4	0.0	0.0	26.93477631336472	0.0
34	5.584288047150537E-4	0.0	0.0	27.57234245726225	0.0
35	5.584288047150537E-4	0.0	0.0	28.274766118050255	0.0
36	5.584288047150537E-4	0.0	0.0	28.98165720927598	0.0
37	6.382043482457757E-4	0.0	0.0	29.632944746660794	0.0
38	7.179798917764977E-4	0.0	0.0	30.260698498704045	0.0
39	7.179798917764977E-4	0.0	0.0	30.898902846949824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	25	3.8910075E-5	45.000004	1
GTTCGCT	25	3.8910075E-5	45.000004	32
TTACACG	140	0.0	45.000004	34
TAATGCG	25	3.8910075E-5	45.000004	24
TCGCAAG	20	7.0335314E-4	45.0	14
ACCGGCC	20	7.0335314E-4	45.0	27
AGATTCG	20	7.0335314E-4	45.0	33
GGACGTA	20	7.0335314E-4	45.0	8
GCGATAT	45	3.8562575E-10	45.0	9
CGTTTCA	20	7.0335314E-4	45.0	17
CGATAAG	20	7.0335314E-4	45.0	20
ATACCGG	20	7.0335314E-4	45.0	11
ACGGTAC	20	7.0335314E-4	45.0	12
AAGATCG	30	2.1656597E-6	44.999996	28
GGCGATA	60	0.0	44.999996	8
CGAACGA	110	0.0	42.954544	22
TCGTTCA	240	0.0	42.187496	16
TCGTTTG	350	0.0	41.785713	1
AACGACA	60	3.6379788E-12	41.249996	10
TAACGAA	115	0.0	41.086956	19
>>END_MODULE