##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557570
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.265727383038964	33.0	31.0	34.0	30.0	34.0
2	32.45610791168294	34.0	31.0	34.0	31.0	34.0
3	32.51945658943097	34.0	31.0	34.0	31.0	34.0
4	35.99644060941081	37.0	35.0	37.0	35.0	37.0
5	35.96106242416071	37.0	35.0	37.0	35.0	37.0
6	35.5888968072061	37.0	35.0	37.0	33.0	37.0
7	35.91581373549825	37.0	35.0	37.0	35.0	37.0
8	36.004223245183205	37.0	35.0	37.0	35.0	37.0
9	37.78422992225069	39.0	38.0	39.0	35.0	39.0
10	37.322407981663744	39.0	37.0	39.0	34.0	39.0
11	37.21151729938301	39.0	37.0	39.0	34.0	39.0
12	36.976851120655894	39.0	37.0	39.0	33.0	39.0
13	36.88992212228022	39.0	37.0	39.0	33.0	39.0
14	37.96249349948959	40.0	37.0	41.0	33.0	41.0
15	38.050411217473375	40.0	37.0	41.0	33.0	41.0
16	38.118727890239285	40.0	37.0	41.0	33.0	41.0
17	38.058170098294134	40.0	37.0	41.0	33.0	41.0
18	38.034112752556865	40.0	37.0	41.0	33.0	41.0
19	38.04740461102872	40.0	37.0	41.0	34.0	41.0
20	37.991387866997954	40.0	36.0	41.0	33.0	41.0
21	37.82154702517383	40.0	36.0	41.0	33.0	41.0
22	37.917520239860806	40.0	36.0	41.0	33.0	41.0
23	37.876688688148846	40.0	36.0	41.0	33.0	41.0
24	37.81758636850992	40.0	36.0	41.0	33.0	41.0
25	37.73903452172294	40.0	36.0	41.0	33.0	41.0
26	37.69296853431948	40.0	36.0	41.0	33.0	41.0
27	37.61199753462124	40.0	36.0	41.0	33.0	41.0
28	37.481446098730714	39.0	35.0	41.0	33.0	41.0
29	37.36976058860918	39.0	35.0	41.0	32.0	41.0
30	37.33822171716199	39.0	35.0	41.0	32.0	41.0
31	37.26193686319074	39.0	35.0	41.0	32.0	41.0
32	37.13369415178772	39.0	35.0	41.0	32.0	41.0
33	37.08056652349493	39.0	35.0	41.0	32.0	41.0
34	36.905689631926656	39.0	35.0	41.0	31.0	41.0
35	36.82643861913108	39.0	35.0	41.0	31.0	41.0
36	36.688523148237316	39.0	35.0	41.0	31.0	41.0
37	36.64352035542544	39.0	35.0	41.0	31.0	41.0
38	36.52092426022586	39.0	35.0	41.0	30.0	41.0
39	36.45190906347708	39.0	35.0	41.0	30.0	41.0
40	36.34293675404637	39.0	35.0	40.0	30.0	41.0
41	36.24867453790199	38.0	35.0	40.0	30.0	41.0
42	36.20338283351631	38.0	35.0	40.0	30.0	41.0
43	36.1215296904794	38.0	35.0	40.0	30.0	41.0
44	35.94583550017013	38.0	35.0	40.0	30.0	41.0
45	35.92638789909924	38.0	35.0	40.0	30.0	41.0
46	35.83593353749751	38.0	35.0	40.0	29.0	41.0
47	35.622767516066695	38.0	34.0	40.0	29.0	41.0
48	35.5413156391045	38.0	34.0	40.0	29.0	41.0
49	35.513197480691076	37.0	34.0	40.0	28.0	41.0
50	35.401439421663234	37.0	34.0	40.0	28.0	41.0
51	34.44900132899324	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	24.0
10	39.0
11	40.0
12	42.0
13	40.0
14	41.0
15	67.0
16	106.0
17	190.0
18	370.0
19	645.0
20	1086.0
21	1696.0
22	2624.0
23	4002.0
24	6007.0
25	8761.0
26	11997.0
27	14771.0
28	17696.0
29	21160.0
30	26078.0
31	33362.0
32	44176.0
33	63134.0
34	108908.0
35	123603.0
36	134435.0
37	201108.0
38	328511.0
39	402705.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.818788240656918	4.820650115243617	52.727517864365645	17.633043779733818
2	20.944098820598754	4.434985265509736	52.10122177494431	22.519694138947205
3	22.099038887497834	4.803315420815758	50.87251295286889	22.225132738817514
4	19.477519469429946	5.018458239437072	49.2230204742002	26.281001816932786
5	18.400457122312318	5.86349249150921	50.285958255487714	25.450092130690756
6	22.21293425014606	6.700950840090654	52.51686922578118	18.5692456839821
7	84.77147094512605	2.4686530942429554	8.524753301617263	4.2351226590137205
8	86.17166483689337	3.042752492664856	6.945947854671059	3.8396348157707196
9	80.82570927791367	5.127731016904537	9.048453681054463	4.998106024127327
10	41.08354680688508	25.964226326906655	17.048094146651515	15.904132719556745
11	30.599395211772183	25.856430208594155	23.932214924529877	19.61195965510378
12	27.464640433495767	22.644439736255833	29.037089825818423	20.853830004429977
13	25.027960220086413	23.015723209871787	30.425534646917956	21.53078192312384
14	20.943328389735292	25.06230859608236	31.50664175606875	22.4877212581136
15	19.231045795694577	24.83933306368253	33.75225511533992	22.177366025282975
16	23.791226076516626	23.662885135178517	30.75027125586651	21.79561753243835
17	23.249805787219834	24.398903420071008	29.117086230474392	23.234204562234765
18	24.613853630976458	23.33956098281297	29.947353890996876	22.099231495213697
19	24.83130774218815	25.645396354577965	28.161109934063962	21.362185969169925
20	26.93041083225794	26.555275204324687	27.558632998837933	18.95568096457944
21	25.433656272270266	26.43097902501974	29.260771586509755	18.874593116200238
22	24.66174874965491	22.791848841464592	29.47899612858491	23.06740628029559
23	23.512073293656144	24.808772639431936	28.661183767021708	23.017970299890216
24	22.548777904042836	25.21793563050136	29.623066700052004	22.6102197654038
25	23.77312095122531	26.35425695153348	26.663135525209142	23.209486572032077
26	22.1917474014009	26.448506327163468	27.29784215155659	24.061904119879042
27	22.55506975609443	25.769178913307268	29.26237665080863	22.413374679789673
28	21.12091270376291	26.210250582638338	29.196183799122995	23.472652914475752
29	21.960874952650602	25.269426093209297	29.60412694132527	23.165572012814835
30	24.93255519816124	23.21577842408367	30.145226217762282	21.70644015999281
31	25.450477346122486	25.243038836135778	27.259320608383568	22.047163209358168
32	24.472607972675387	24.948734246293906	27.924330848693803	22.65432693233691
33	25.205672939257944	24.089639630963617	26.92360535963071	23.78108207014773
34	22.358032062764437	24.410716693310732	30.017398896999815	23.21385234692502
35	22.312640844392227	24.68852122216016	29.53048659129285	23.468351342154765
36	24.632857592275144	26.219945171003552	27.279544418549406	21.867652818171894
37	24.485641094782256	25.70780125451825	27.539757442683154	22.26680020801633
38	23.903516374865976	25.761924022676347	27.941151922546016	22.393407679911657
39	23.5785229556296	23.09841612254987	28.282902213062656	25.040158708757872
40	25.63223482732718	23.353557143499167	28.676335574003094	22.337872455170555
41	21.65507810242878	23.70480941466515	28.64872846806243	25.991384014843632
42	23.17423936002876	23.75758392881219	28.482508009270852	24.585668701888196
43	23.04294510038072	23.26219688360716	29.653498719158687	24.041359296853432
44	23.680476639894195	23.37243269965395	28.725193731260873	24.221896929190983
45	24.199747041866498	22.572982273669883	27.853772222115218	25.373498462348405
46	23.707762732975084	23.44491740339118	28.678197448589792	24.169122415043947
47	21.69115994786751	25.30383867177719	30.43709110986986	22.567910270485434
48	22.35591337788992	24.74591832148796	30.27164108194174	22.62652721868038
49	24.615779708135108	22.981310631303888	29.918013315613422	22.48489634494758
50	22.778237896210122	23.311440256296667	29.070089947803307	24.8402318996899
51	22.093196453449927	23.162490289360992	27.54438002786392	27.19993322932517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	437.0
1	1507.5
2	2578.0
3	5462.5
4	8347.0
5	5845.0
6	3343.0
7	3337.0
8	3331.0
9	3480.0
10	3629.0
11	3669.0
12	3709.0
13	3613.0
14	3517.0
15	3579.0
16	3641.0
17	3857.5
18	4074.0
19	4080.5
20	4087.0
21	4526.5
22	4966.0
23	5586.5
24	6207.0
25	7382.5
26	11396.5
27	14235.0
28	17509.5
29	20784.0
30	24199.5
31	27615.0
32	30027.5
33	32440.0
34	36832.0
35	41224.0
36	46545.5
37	51867.0
38	53115.0
39	54363.0
40	60286.0
41	66209.0
42	70730.0
43	75251.0
44	81943.0
45	88635.0
46	94031.0
47	99427.0
48	108643.0
49	117859.0
50	129951.5
51	142044.0
52	142037.5
53	142031.0
54	128002.0
55	113973.0
56	104573.0
57	95173.0
58	87439.5
59	79706.0
60	72500.0
61	65294.0
62	56550.0
63	47806.0
64	42447.5
65	37089.0
66	32755.5
67	28422.0
68	23865.5
69	19309.0
70	16470.5
71	13632.0
72	11427.5
73	9223.0
74	7893.5
75	5045.0
76	3526.0
77	2507.0
78	1488.0
79	1303.5
80	1119.0
81	742.5
82	366.0
83	333.0
84	300.0
85	188.0
86	76.0
87	77.0
88	78.0
89	47.0
90	16.0
91	9.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1557570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.270409176600495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74496267037024	18.130245630401838
2	11.4416686005044	5.782712943956162
3	4.441389634774398	3.3670720015038382
4	2.275742509868021	2.300357776197947
5	1.2977138906676373	1.6396880505664697
6	0.8570760270947322	1.2995197140083408
7	0.5960600302585964	1.0543876600916122
8	0.4491691889513361	0.9080551355457637
9	0.35099392895208187	0.7982784182809576
>10	4.895225482653113	32.233218515198665
>50	1.529975877386529	25.400352699765406
>100	0.11281052672331315	4.893818678266228
>500	0.005408723846672643	0.9525991471278972
>1k	0.0018029079488908813	1.2396936290888945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4564	0.2930205384027684	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	3545	0.22759811758059026	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	3345	0.21475760318958376	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2701	0.17341114685054282	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2099	0.13476119853361326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2840514391006505E-4	0.0	0.0	0.23279852590894792	0.0
2	1.2840514391006505E-4	0.0	0.0	0.8348902457032429	0.0
3	1.2840514391006505E-4	0.0	0.0	1.1696424558767824	0.0
4	1.2840514391006505E-4	0.0	0.0	1.5724493923226563	0.0
5	1.2840514391006505E-4	0.0	0.0	2.2682126645993437	0.0
6	1.2840514391006505E-4	0.0	0.0	2.7784305039259873	0.0
7	1.2840514391006505E-4	0.0	0.0	3.2234185301463176	0.0
8	1.2840514391006505E-4	0.0	0.0	3.8845124135672875	0.0
9	1.2840514391006505E-4	0.0	0.0	4.2203560674640626	0.0
10	1.2840514391006505E-4	0.0	0.0	4.886457751497525	0.0
11	1.2840514391006505E-4	0.0	0.0	6.27965356292173	0.0
12	1.9260771586509755E-4	0.0	0.0	7.47298676784992	0.0
13	1.9260771586509755E-4	0.0	0.0	7.873289803989548	0.0
14	1.9260771586509755E-4	0.0	0.0	8.01851602175183	0.0
15	1.9260771586509755E-4	0.0	0.0	8.239950692424738	0.0
16	1.9260771586509755E-4	0.0	0.0	8.755689952939514	0.0
17	1.9260771586509755E-4	0.0	0.0	9.44567499374025	0.0
18	2.568102878201301E-4	0.0	0.0	10.225222622418254	0.0
19	2.568102878201301E-4	0.0	0.0	10.761956123962326	0.0
20	2.568102878201301E-4	0.0	0.0	11.356664547981792	0.0
21	2.568102878201301E-4	0.0	0.0	12.161700597725945	0.0
22	2.568102878201301E-4	0.0	0.0	12.988822332222629	0.0
23	2.568102878201301E-4	0.0	0.0	13.740185031812375	0.0
24	2.568102878201301E-4	0.0	0.0	14.348375995942398	0.0
25	3.210128597751626E-4	0.0	0.0	14.889924690383097	0.0
26	3.210128597751626E-4	0.0	0.0	15.35141277759587	0.0
27	4.494180036852276E-4	0.0	0.0	15.874920549317206	0.0
28	4.494180036852276E-4	0.0	0.0	16.37351772311999	0.0
29	4.494180036852276E-4	0.0	0.0	16.960714446220717	0.0
30	4.494180036852276E-4	0.0	0.0	17.624504837663796	0.0
31	4.494180036852276E-4	0.0	0.0	18.165668316672765	0.0
32	4.494180036852276E-4	0.0	0.0	18.701438779637513	0.0
33	4.494180036852276E-4	0.0	0.0	19.23990575062437	0.0
34	4.494180036852276E-4	0.0	0.0	19.781005027061383	0.0
35	5.136205756402602E-4	0.0	0.0	20.395295235527136	0.0
36	5.136205756402602E-4	0.0	0.0	20.91167652176146	0.0
37	5.136205756402602E-4	0.0	0.0	21.469982087482425	0.0
38	5.136205756402602E-4	0.0	0.0	22.06533253722144	0.0
39	5.136205756402602E-4	0.0	0.0	22.906322027260412	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCCGT	30	2.1659998E-6	45.000004	18
CACGTTG	30	2.1659998E-6	45.000004	32
CGATTAC	20	7.0340605E-4	45.0	12
CCGTCGA	20	7.0340605E-4	45.0	2
CGTAAGA	20	7.0340605E-4	45.0	35
ATCGGAT	20	7.0340605E-4	45.0	24
TAGGACG	20	7.0340605E-4	45.0	1
CGTCATA	40	6.8175723E-9	45.0	38
TACGGGA	85	0.0	42.352943	4
CGTTGAT	110	0.0	40.90909	25
CGTTCAT	265	0.0	40.75472	17
CGTTTTT	2525	0.0	40.09901	1
GCGCGAC	695	0.0	39.820145	9
TCGTTCA	275	0.0	39.272728	16
TCGTTGA	115	0.0	39.130432	24
TTTGGGA	6805	0.0	39.048496	4
AACACGT	415	0.0	39.036144	41
TTTCGCG	480	0.0	38.437504	1
TACGAAT	220	0.0	37.840908	12
TGGGCGA	1600	0.0	37.828125	6
>>END_MODULE