##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1227500
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08652301425662	33.0	31.0	34.0	30.0	34.0
2	32.28905010183299	34.0	31.0	34.0	30.0	34.0
3	32.347708350305496	34.0	31.0	34.0	30.0	34.0
4	35.86495397148676	37.0	35.0	37.0	35.0	37.0
5	35.82994297352342	37.0	35.0	37.0	35.0	37.0
6	35.47361710794297	37.0	35.0	37.0	33.0	37.0
7	35.83508024439919	37.0	35.0	37.0	35.0	37.0
8	35.92651568228106	37.0	35.0	37.0	35.0	37.0
9	37.684773116089616	39.0	38.0	39.0	35.0	39.0
10	37.15311771894093	39.0	37.0	39.0	34.0	39.0
11	37.0435266802444	39.0	37.0	39.0	33.0	39.0
12	36.899319755600814	39.0	37.0	39.0	33.0	39.0
13	36.841487576374746	39.0	37.0	39.0	33.0	39.0
14	37.963217107942974	40.0	37.0	41.0	33.0	41.0
15	37.99585743380855	40.0	37.0	41.0	33.0	41.0
16	38.02292301425662	40.0	37.0	41.0	33.0	41.0
17	37.95307535641548	40.0	37.0	41.0	33.0	41.0
18	37.91552993890021	40.0	37.0	41.0	33.0	41.0
19	37.965489205702646	40.0	37.0	41.0	33.0	41.0
20	37.91105824847251	40.0	36.0	41.0	33.0	41.0
21	37.753472912423625	39.0	36.0	41.0	33.0	41.0
22	37.82735641547862	39.0	36.0	41.0	33.0	41.0
23	37.78729124236253	39.0	36.0	41.0	33.0	41.0
24	37.746622403258655	39.0	36.0	41.0	33.0	41.0
25	37.67658737270876	39.0	36.0	41.0	33.0	41.0
26	37.58415967413442	39.0	36.0	41.0	33.0	41.0
27	37.513046843177186	39.0	36.0	41.0	33.0	41.0
28	37.386476578411404	39.0	36.0	41.0	32.0	41.0
29	37.30923747454175	39.0	36.0	41.0	32.0	41.0
30	37.31121466395112	39.0	36.0	41.0	32.0	41.0
31	37.259524236252545	39.0	35.0	41.0	32.0	41.0
32	37.14070549898167	39.0	35.0	41.0	32.0	41.0
33	37.08983217922607	39.0	35.0	41.0	31.0	41.0
34	36.93367739307536	39.0	35.0	41.0	31.0	41.0
35	36.8654916496945	39.0	35.0	41.0	31.0	41.0
36	36.711849287169045	39.0	35.0	41.0	31.0	41.0
37	36.70340936863544	39.0	35.0	41.0	31.0	41.0
38	36.64270468431772	39.0	35.0	41.0	30.0	41.0
39	36.603873727087574	39.0	35.0	41.0	30.0	41.0
40	36.46375967413442	39.0	35.0	40.0	30.0	41.0
41	36.3694199592668	39.0	35.0	40.0	30.0	41.0
42	36.287340122199595	39.0	35.0	40.0	30.0	41.0
43	36.20930264765784	39.0	35.0	40.0	30.0	41.0
44	36.04854745417515	38.0	35.0	40.0	29.0	41.0
45	36.09382484725051	38.0	35.0	40.0	30.0	41.0
46	36.00121466395112	38.0	35.0	40.0	29.0	41.0
47	35.791903869653765	38.0	35.0	40.0	28.0	41.0
48	35.72598778004073	38.0	34.0	40.0	28.0	41.0
49	35.74669735234216	38.0	34.0	40.0	28.0	41.0
50	35.64037067209776	38.0	34.0	40.0	28.0	41.0
51	34.68636252545825	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	17.0
10	25.0
11	17.0
12	24.0
13	17.0
14	28.0
15	34.0
16	61.0
17	106.0
18	235.0
19	418.0
20	712.0
21	1213.0
22	1896.0
23	2949.0
24	4364.0
25	6612.0
26	9313.0
27	12074.0
28	14577.0
29	17590.0
30	21726.0
31	27954.0
32	36376.0
33	50047.0
34	80485.0
35	97186.0
36	108169.0
37	157799.0
38	264080.0
39	311291.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.402769857433807	4.6490427698574335	55.505906313645625	17.442281059063134
2	21.193075356415477	4.574501018329939	52.19845213849287	22.03397148676171
3	21.52154786150713	4.698085539714867	50.99706720977597	22.78329938900204
4	19.70354378818737	5.041384928716904	50.014582484725054	25.24048879837067
5	17.857189409368633	5.87877800407332	50.43845213849287	25.82558044806517
6	21.57539714867617	6.403910386965377	53.913075356415476	18.107617107942975
7	82.74077393075356	2.353808553971487	9.469246435845214	5.436171079429735
8	83.93906313645621	2.79877800407332	8.008553971486762	5.253604887983706
9	78.33246435845214	5.544032586558044	10.6189816700611	5.504521384928717
10	37.02981670061099	30.971405295315684	17.731731160896132	14.267046843177189
11	26.31103869653768	25.843584521384926	29.29262729124236	18.55274949083503
12	25.286924643584523	21.487902240325866	32.39128309572302	20.8338900203666
13	24.270631364562117	22.00195519348269	33.168961303462325	20.55845213849287
14	19.75397148676171	25.57401221995927	32.2887983706721	22.383217922606924
15	17.709164969450104	25.7132382892057	35.20448065173116	21.373116089613035
16	20.427372708757638	24.32244399185336	33.261832993890025	21.988350305498983
17	19.986720977596743	24.270875763747455	30.788513238289205	24.953890020366597
18	19.724806517311606	24.618655804480653	32.61002036659878	23.04651731160896
19	22.515030549898167	26.167820773930757	30.621425661914458	20.69572301425662
20	24.1118533604888	26.615804480651732	30.26663951120163	19.00570264765784
21	24.04855397148676	26.322688391038696	30.618329938900203	19.01042769857434
22	21.454908350305498	24.389816700610996	30.418900203665988	23.736374745417514
23	20.936700610997963	25.068431771894094	29.60651731160896	24.38835030549898
24	21.87682281059063	24.518207739307535	30.120977596741344	23.48399185336049
25	21.40496945010183	25.65254582484725	28.49010183299389	24.452382892057027
26	19.1640733197556	28.79340122199593	28.293604887983708	23.74892057026477
27	19.71600814663951	27.072097759674136	30.279592668024442	22.93230142566191
28	17.85816700610998	26.390142566191443	32.26908350305499	23.482606924643584
29	18.983951120162935	25.417433808553973	31.971079429735234	23.627535641547862
30	20.6508350305499	24.421670061099796	32.12268839103869	22.80480651731161
31	21.741588594704684	27.183706720977597	28.66126272912424	22.413441955193484
32	21.666232179226068	27.070061099796334	27.833238289205703	23.430468431771896
33	21.924643584521387	25.42183299389002	28.360977596741343	24.29254582484725
34	20.63918533604888	24.8420366598778	30.58761710794297	23.931160896130347
35	19.934663951120164	24.84570264765784	29.079348268839105	26.14028513238289
36	20.282362525458247	27.062810590631365	28.661670061099798	23.99315682281059
37	19.96448065173116	26.399429735234214	30.490264765784115	23.14582484725051
38	19.61254582484725	25.634704684317715	31.246680244399183	23.506069246435846
39	21.265417515274947	23.323095723014255	32.40790224032587	23.00358452138493
40	22.940448065173115	22.97588594704684	30.78134419551935	23.302321792260692
41	20.326272912423626	24.424114052953158	28.472830957230144	26.776782077393076
42	21.813523421588595	24.753319755600813	27.109735234215886	26.3234215885947
43	21.829816700610998	23.487494908350307	29.436171079429734	25.24651731160896
44	20.515926680244398	22.927006109979633	30.792993890020366	25.7640733197556
45	21.19674134419552	22.620529531568227	29.93327902240326	26.249450101832995
46	21.209450101832992	24.077148676171078	30.37621181262729	24.337189409368634
47	18.948268839103868	24.352993890020365	31.814826883910385	24.883910386965375
48	18.511853360488796	22.97702647657841	32.9383299389002	25.572790224032588
49	21.352830957230143	21.465661914460284	33.56798370672098	23.613523421588596
50	20.550794297352343	21.077800407331974	30.91576374745417	27.45564154786151
51	19.421914460285134	21.38191446028513	29.545661914460286	29.650509164969453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	1196.0
2	2033.0
3	4315.0
4	6597.0
5	4544.5
6	2492.0
7	2618.5
8	2745.0
9	2741.0
10	2737.0
11	2748.5
12	2760.0
13	2763.5
14	2767.0
15	2705.0
16	2643.0
17	2786.5
18	2930.0
19	3334.0
20	3738.0
21	3782.5
22	3827.0
23	5301.5
24	6776.0
25	8061.0
26	11341.5
27	13337.0
28	15527.5
29	17718.0
30	20769.5
31	23821.0
32	26750.0
33	29679.0
34	32748.0
35	35817.0
36	38040.0
37	40263.0
38	44818.5
39	49374.0
40	54293.5
41	59213.0
42	65790.0
43	72367.0
44	80458.0
45	88549.0
46	99940.5
47	111332.0
48	125433.5
49	139535.0
50	133346.0
51	127157.0
52	108473.5
53	89790.0
54	76000.5
55	62211.0
56	56228.5
57	50246.0
58	46272.5
59	42299.0
60	39764.0
61	37229.0
62	32908.5
63	28588.0
64	24422.0
65	20256.0
66	16245.5
67	12235.0
68	10125.0
69	8015.0
70	7190.0
71	6365.0
72	5353.0
73	4341.0
74	3756.5
75	2343.0
76	1514.0
77	1104.0
78	694.0
79	508.0
80	322.0
81	237.5
82	153.0
83	106.0
84	59.0
85	52.5
86	46.0
87	43.5
88	41.0
89	23.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1227500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.365560287640218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4601078209415	18.734492078723243
2	12.455734551472776	6.817163095903431
3	5.499515954338731	4.514920062038873
4	2.9790576546856684	3.2609432739862694
5	1.756827032835053	2.403827804100186
6	1.1471092547499977	1.8834772480422663
7	0.8024799685504452	1.5372219771293585
8	0.5736692492914647	1.2559024341320706
9	0.45887241929573885	1.1301570769115576
>10	4.704587088789958	31.251417765851386
>50	1.039356796857683	18.764199922252295
>100	0.11548325221019051	5.4326988566856205
>500	0.003899434489599784	0.7244802384946022
>1k	0.0032995214911998175	2.2890981657488068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	4742	0.38631364562118126	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	4480	0.364969450101833	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	4324	0.3522606924643584	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3667	0.29873727087576374	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3015	0.2456211812627291	No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1843	0.15014256619144603	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1385	0.11283095723014257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.702647657841141E-4	0.0	0.0	0.37784114052953155	0.0
2	5.702647657841141E-4	0.0	0.0	1.4992260692464359	0.0
3	5.702647657841141E-4	0.0	0.0	2.2327494908350305	0.0
4	5.702647657841141E-4	0.0	0.0	3.0725865580448066	0.0
5	5.702647657841141E-4	0.0	0.0	4.318044806517312	0.0
6	5.702647657841141E-4	0.0	0.0	5.143625254582485	0.0
7	5.702647657841141E-4	0.0	0.0	5.906720977596741	0.0
8	5.702647657841141E-4	0.0	0.0	7.081547861507128	0.0
9	5.702647657841141E-4	0.0	0.0	7.647657841140529	0.0
10	5.702647657841141E-4	0.0	0.0	8.737433808553972	0.0
11	5.702647657841141E-4	0.0	0.0	11.029816700610999	0.0
12	5.702647657841141E-4	0.0	0.0	13.094419551934827	0.0
13	5.702647657841141E-4	0.0	0.0	13.75682281059063	0.0
14	5.702647657841141E-4	0.0	0.0	13.978655804480653	0.0
15	5.702647657841141E-4	0.0	0.0	14.31918533604888	0.0
16	5.702647657841141E-4	0.0	0.0	15.157474541751528	0.0
17	5.702647657841141E-4	0.0	0.0	16.344439918533606	0.0
18	5.702647657841141E-4	0.0	0.0	17.684317718940935	0.0
19	5.702647657841141E-4	0.0	0.0	18.562281059063135	0.0
20	6.517311608961303E-4	0.0	0.0	19.556496945010185	0.0
21	6.517311608961303E-4	0.0	0.0	20.826395112016293	0.0
22	6.517311608961303E-4	0.0	0.0	22.0271283095723	0.0
23	6.517311608961303E-4	0.0	0.0	23.18908350305499	0.0
24	6.517311608961303E-4	0.0	0.0	24.09417515274949	0.0
25	6.517311608961303E-4	0.0	0.0	24.89458248472505	0.0
26	6.517311608961303E-4	0.0	0.0	25.59380855397149	0.0
27	6.517311608961303E-4	0.0	0.0	26.23160896130346	0.0
28	6.517311608961303E-4	0.0	0.0	26.92806517311609	0.0
29	6.517311608961303E-4	0.0	0.0	27.6989816700611	0.0
30	6.517311608961303E-4	0.0	0.0	28.53621181262729	0.0
31	6.517311608961303E-4	0.0	0.0	29.326680244399185	0.0
32	6.517311608961303E-4	0.0	0.0	30.04529531568228	0.0
33	6.517311608961303E-4	0.0	0.0	30.789816700611	0.0
34	6.517311608961303E-4	0.0	0.0	31.491812627291242	0.0
35	6.517311608961303E-4	0.0	0.0	32.267209775967416	0.0
36	6.517311608961303E-4	0.0	0.0	32.99470468431772	0.0
37	7.331975560081466E-4	0.0	0.0	33.723747454175154	0.0
38	7.331975560081466E-4	0.0	0.0	34.425661914460285	0.0
39	7.331975560081466E-4	0.0	0.0	35.165376782077395	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	70	0.0	45.000004	2
TCGCAAG	20	7.033474E-4	45.0	1
TTATCCG	20	7.033474E-4	45.0	1
ATCCGGT	20	7.033474E-4	45.0	16
TGCGCAT	20	7.033474E-4	45.0	13
CTACCGT	20	7.033474E-4	45.0	19
TATAGCG	20	7.033474E-4	45.0	1
ATACCGA	20	7.033474E-4	45.0	10
CGACAAA	30	2.1656233E-6	44.999996	10
ACGGGTA	30	2.1656233E-6	44.999996	5
CACTCGA	30	2.1656233E-6	44.999996	44
TGCGACA	30	2.1656233E-6	44.999996	30
CGGTAGT	50	1.0822987E-9	40.5	12
TTGGGTA	805	0.0	39.68944	5
CGAACTC	40	3.458481E-7	39.375	13
CGGTACA	40	3.458481E-7	39.375	20
TTGGGAT	4065	0.0	39.18819	5
TTTGGGC	2645	0.0	39.04537	4
CGTTTTT	1925	0.0	38.922077	1
CTTGGGA	3615	0.0	38.900414	4
>>END_MODULE